RESUMO
Evidence of cerebellar abiotrophy (CA) was found in a six-month-old Arabian filly with signs of incoordination, head tremor, wobbling, loss of balance and falling over, consistent with a cerebellar lesion. Normal hematology profile blood test and cerebrospinal fluid analysis excluded infectious encephalitis, and serological testing for Sarcocystis neurona was negative. The filly was euthanized. Postmortem X-ray radiography of the cervical cephalic region identified not abnormalities, discounting spinal trauma. The histopathological analysis of serial transverse cerebellar sections by electron microscopy revealed morphological characteristics of apoptotic cells with pyknotic nuclei and degenerate mitochondria, cytoplasmic condensation and areas with absence of Purkinje cells, matching with CA histopathological characteristics. The indirect DNA test for CA was positive in the filly, and DNA test confirmed the CA carrier state in the parents and the recessive inheritance of the disease. To our knowledge this is the first report of a CA case in Argentina.
RESUMO
The prognostic significance of angiogenesis in some canine tumours has been investigated, but little is known about its relevance in canine melanocytic tumours (MTs). The aim of this study was to evaluate the prognostic significance of angiogenesis in canine MTs. A total of 36 cutaneous melanocytomas (benign MTs), 40 cutaneous melanomas (malignant MTs) and 43 oral melanomas were studied. Survival data were available for a subset of 59 cases. Microvessel density (MVD) and endothelial area (EA) were determined by immunolabelling using an antibody specific for von Willebrand factor (vWF). Mean MVD (expressed as the number of microvessels per mm(2)) was 129 ± 14 in melanocytomas, 191 ± 16 in cutaneous melanomas and 208 ± 16 in oral melanomas. Mean EA (expressed as the percentage of the total area) was 1.5 ± 0.14 in melanocytomas, 2.6 ± 0.2 in cutaneous melanomas and 2.4 ± 0.3 in oral melanomas. The differences in MVD and EA between melanocytomas and melanomas were significant (P = 0.001 and P = 0.003, respectively). MVD and EA were significantly correlated between cutaneous and oral MTs (r = 0.54; P <0.001 and r = 0.63; P <0.001, respectively). MVD and EA were not related to survival in cutaneous and oral MTs. In conclusion, tumour vascularization was higher in melanomas than in melanocytomas, but it seemed to have no prognostic significance in these tumours.
Assuntos
Doenças do Cão/diagnóstico , Melanoma/veterinária , Neoplasias Bucais/veterinária , Neovascularização Patológica/veterinária , Neoplasias Cutâneas/veterinária , Animais , Argentina/epidemiologia , Doenças do Cão/metabolismo , Doenças do Cão/mortalidade , Cães , Melanócitos/patologia , Melanoma/irrigação sanguínea , Melanoma/diagnóstico , Melanoma/mortalidade , Microvasos/patologia , Neoplasias Bucais/irrigação sanguínea , Neoplasias Bucais/diagnóstico , Neoplasias Bucais/mortalidade , Neovascularização Patológica/diagnóstico , Prognóstico , Neoplasias Cutâneas/irrigação sanguínea , Neoplasias Cutâneas/diagnóstico , Neoplasias Cutâneas/mortalidade , Taxa de SobrevidaRESUMO
This report describes an alternative technique to inoculate rabbits and to reproduce infection by Bovine herpesvirus 1 and 5. First, the nostrils are anaesthetized by aspersion with local anaesthetic. A few seconds later, and after proving the insensitivity of the zone, the rabbits are put on their back legs with their nostrils upwards and the inoculum is introduced slowly into each nostril by using disposable droppers. Clinical signs, viral isolation from nasal swabs, histological lesions found, positive polymerase chain reaction and antibodies production confirm the infection. This very simple and bloodless technique, where the animals are exposed to minor distress, may be useful for evaluating the virulence of BoHV-1 and BoHV-5 strains, to study the establishment of latent virus infection and to test the potential of experimental vaccines or properties of antiviral drugs. It may be also suitable for experimental infection with other respiratory viruses in this animal model.
Assuntos
Encefalite Viral/virologia , Infecções por Herpesviridae/virologia , Herpesvirus Bovino 1/crescimento & desenvolvimento , Herpesvirus Bovino 5/crescimento & desenvolvimento , Meningoencefalite/virologia , Virologia/métodos , Administração Intranasal , Animais , Anticorpos Antivirais/sangue , Modelos Animais de Doenças , Encefalite Viral/fisiopatologia , Feminino , Infecções por Herpesviridae/fisiopatologia , Herpesvirus Bovino 1/isolamento & purificação , Herpesvirus Bovino 5/isolamento & purificação , Fígado/patologia , Pulmão/patologia , Masculino , Meningoencefalite/fisiopatologia , Nariz/virologia , CoelhosRESUMO
A 10-year-old male Briard dog was presented because of listlessness, abnormal gait, fever, inappetence, and seizures. A non-pigmented growth was observed in the ventral quadrant of the left iris. Thoracic radiographs revealed multiple pulmonary metastases and the owner opted for killing. On necropsy, lung masses and nodules in left iris, right adrenal medulla, and brain were detected. Histologically the primary tumour was diagnosed as pulmonary adenocarcinoma with predominant solid pattern. Metastases to regional lymph nodes, uvea, adrenal medulla, and brain were recognized. The metastatic behaviour resembled that occurring in humans. To the authors' knowledge, this is the first report of a pulmonary adenocarcinoma with metastasis to the uvea in a dog.
Assuntos
Adenocarcinoma/veterinária , Neoplasias Encefálicas/veterinária , Doenças do Cão/patologia , Neoplasias Pulmonares/veterinária , Neoplasias Uveais/veterinária , Adenocarcinoma/patologia , Animais , Neoplasias Encefálicas/secundário , Doenças do Cão/diagnóstico , Cães , Evolução Fatal , Neoplasias Pulmonares/patologia , Masculino , Metástase Neoplásica , Neoplasias Uveais/secundárioRESUMO
Lymphangiosarcoma is a rare tumour in domestic animals arising from lymphatic endothelial cells. Occasionally, microscopic differentiation with haemangiosarcoma may be difficult. The aim of the present study was to describe a lymphangiosarcoma in a 1-year-old female Doberman Pinscher dog and to characterize its lectinhistochemical binding pattern as compared with that of haemangiosarcoma. The dog was presented because of a cutaneous painful swelling located in the left axilla. Histological diagnosis confirmed lymphangiosarcoma. The dog was killed. Necropsy revealed mediastinal lymph nodes' involvement. Twenty lectins were tested in tissue sections of this case as well as in four haemangiosarcomas from other dogs. Staining intensity was issued upon optical density determinations. Percentage of lectinhistochemical staining area was also conducted. RCA-I showed the most intense and wide distributed labelling pattern for lymphangiosarcoma. PHA-E was the counterpart for haemangiosarcoma. Should similar results be obtained in further studies, such differences could aid in the differential diagnosis between lymphangiosarcoma and haemangiosarcoma when histological pictures were not conclusive.
Assuntos
Doenças do Cão/patologia , Lectinas , Linfangiossarcoma/veterinária , Neoplasias Cutâneas/veterinária , Animais , Axila , Diagnóstico Diferencial , Doenças do Cão/diagnóstico , Cães , Feminino , Imuno-Histoquímica/veterinária , Linfangiossarcoma/diagnóstico , Linfangiossarcoma/patologia , Neoplasias Cutâneas/diagnóstico , Neoplasias Cutâneas/patologiaRESUMO
Peripheral neuroblastoma (PNB) is a rare neoplasia derived from neuroepithelial cells. PNB typically presents as a greyish mass, composed of round cells with features of neuronal differentiation. Necropsy, performed on a 1-day-old piglet, revealed a mass craniodorsally located in the abdominal cavity. Histologically, the predominant population consisted of small round to ovoid cells with scanty cytoplasm and dark round nuclei, besides, there were larger neurone-like cells. Neurone-specific enolase and S-100 protein were immunohistochemically detected, while glial fibrillary acidic protein was negative. Histological and immunohistochemical findings substantiated the diagnosis of a grade II peripheral neuroblastoma. This seems to be the first description of a PNB in a newborn piglet.
Assuntos
Neoplasias Abdominais/veterinária , Neuroblastoma/veterinária , Doenças dos Suínos/diagnóstico , Neoplasias Abdominais/diagnóstico , Animais , Animais Recém-Nascidos , Autopsia , Diagnóstico Diferencial , Imuno-Histoquímica/veterinária , Masculino , Neuroblastoma/diagnóstico , Suínos , Doenças dos Suínos/patologiaRESUMO
In the present study, we compared the utility of immunohistochemistry with serological and histological results for the characterization of Mycobacterium avium ssp. paratuberculosis (M. paratuberculosis) in tissues of affected red deer. Bacterial isolation was considered the standard reference. Samples were taken from seven clinically affected animals with typical macroscopic lesions. The enzyme linked immunosorbent assay (ELISA) and the gel diffusion tests (GD) were used for serological determinations. Samples from intestine and mesenteric lymph nodes were processed for bacterial isolation and histology. M. paratuberculosis was isolated from all the animals. Histologically, lymph nodes displayed necrosis and mineralization at the cortical and medullar areas. Ziehl-Neelsen stained bacteria were numerous inside macrophages and Langhans-type giant cells. Giant and epithelioid cells and lymphocytes were prominent at the ileal mucous membrane. The immunostaining of M. paratuberculosis was very clear inside epithelioid and giant cells. Image analysis was carried out to determine the immunostained area. There was total agreement among the methods employed. Immunohistochemistry can be very useful when the microorganism cannot be recovered from tissues or faeces.
Assuntos
Cervos , Imuno-Histoquímica/veterinária , Mycobacterium avium subsp. paratuberculosis/imunologia , Paratuberculose/microbiologia , Animais , Anticorpos Antibacterianos/sangue , DNA Bacteriano/análise , Ensaio de Imunoadsorção Enzimática/veterinária , Fezes/microbiologia , Imunodifusão/veterinária , Imuno-Histoquímica/métodos , Linfonodos/microbiologia , Mycobacterium avium subsp. paratuberculosis/genética , Mycobacterium avium subsp. paratuberculosis/isolamento & purificação , Paratuberculose/patologiaRESUMO
Solanum glaucophyllum (Sg) (synonym S. malacoxylon) is a plant toxic to cattle due to its high levels of 1,25-dihydroxyvitamin D3 as glycoside derivatives. Sg causes a disease characterized by wasting and calcification of soft tissues. The effects of vitamin D are not only important in calcium homeostasis, but also in immune regulation, cell growth and cell differentiation. Skin samples in Sg-intoxicated and control heifers were studied histologically. Cellular differentiation and proliferation were analysed by immunohistochemical expression of cytokeratins, involucrin and proliferating cell nuclear antigen (PCNA). The results were obtained by image processing and analysis and were statistically evaluated. Sg-intoxicated cattle showed atrophy of epidermis and severe involution of hair follicles and of sebaceous and sweat glands. As judged by PCNA expression, cellular proliferation was reduced, even though the reduction was not statistically significant. The analysed markers of differentiation, e.g. involucrin and cytokeratins 10 and 11, changed in relation to Sg-poisoning. The possible pathogenesis of the skin lesions is discussed.
Assuntos
Doenças dos Bovinos/patologia , Intoxicação por Plantas/veterinária , Dermatopatias/veterinária , Solanaceae/intoxicação , Vitamina D/toxicidade , Animais , Anticorpos Monoclonais , Argentina , Peso Corporal , Bovinos , Doenças dos Bovinos/etiologia , Diferenciação Celular/efeitos dos fármacos , Divisão Celular/efeitos dos fármacos , Feminino , Processamento de Imagem Assistida por Computador , Imuno-Histoquímica , Queratinas/análise , Antígeno Nuclear de Célula em Proliferação/análise , Precursores de Proteínas/análise , Dermatopatias/etiologia , Dermatopatias/patologia , Alcaloides de Solanáceas/toxicidadeRESUMO
La estructura de las vísceras y glándulas anexas del aparato digestivo de los camélidos sudamericanos, muestran diferencias morfológicas con relación a otros rumiantes. Los estudios macroscópicos realizados, nos permitieron observar que en los camélidos sudamericanos la ampolla duodenal y el conducto hepatopancreático (CHP) presentan características particulares que le confieren una marcada diferencia con los bovinos domésticos, al presentar una desembocadura común de ambos conductos. Los estudios se realizaron mediante técnicas histológicas de rutina y por medio de histoquímica de lectinas. El conducto biliar se une al pancreático a unos 3 cm del duodeno, formando el CHP que desemboca sobre el duodeno en el pliegue duodenal, bien visible, con dirección caudal a favor del tránsito intestinal, recorriendo 2 cm por dentro de la mucosa intestinal. Presenta un orificio terminal en forma de bisel, ubicado a unos 40 cm del píloro. No se observa conducto pancreático accesorio. El CHP se halla tapizado por epitelio cilíndroco simple, sin células caliciformes. Sobre el pliegue duodenal se observa mayor desarrollo glandular, aumentando su espesor. El epitelio cilíndrico es más alto, más acidófilo y se invagina formando glándulas rodeadas de tejido conectivo. Las glándulas son tubulares con células acidófilas y basófilas, ricas en mucopolisacáridos. Para caracterizar los glicoconjugados, se utilizó una batería de siete lectinas (con A, UEA-1, SBA, DBA, PNA, RCA-1, WGA). En las células epiteliales de la mucosa, los glucoconjugados más destacados presentes son la N-acetilglucosamina y N-acetilgalactosamina dado que dieron marcada reactividad con las lectinas WGA y SBA. La N-acetilglucosamina y N-acetilgalactosamina son aminoazúcares que suelen encontrarse como carbohidratos estructurales. En las glándulas de la mucosa del CHP se observó que las células presentan N-acetilgalactosamina y en menor proporción N-glucosamina
Assuntos
Animais , Masculino , Feminino , Camelídeos Americanos/anatomia & histologia , Sistema Digestório/anatomia & histologia , Epitélio/anatomia & histologia , Fígado/anatomia & histologia , Técnicas Histológicas , Lectinas , Pâncreas/anatomia & histologiaRESUMO
Histochemical and lectinhistochemical examinations were carried out on nasal mucosa of pigs with or without respiratory diseases. As the results, both acid and neutral mucins coexisted in nasal mucosa of normal pigs while acid sialomucins were mainly observed in nasal mucosa of pigs infected with Bordetella bronchiseptica and/or Pasteurella multocida. Lectinhistochemistry revealed that the nasal epithelial cells of normal pigs were rich in N-acetylgalactosamine, fucose and N-acetyl-glucosamine residues which showed a tendency to disappear in porcine cytomegalovirus infection and to increase in atrophic rhinitis, respectively.
Assuntos
Infecções por Bordetella/veterinária , Bordetella bronchiseptica , Lectinas/metabolismo , Mucosa Nasal/metabolismo , Infecções por Pasteurella/veterinária , Pasteurella multocida , Rinite Atrófica/veterinária , Doenças dos Suínos/metabolismo , Animais , Infecções por Bordetella/metabolismo , Infecções por Bordetella/microbiologia , Infecções por Citomegalovirus/metabolismo , Infecções por Citomegalovirus/veterinária , Infecções por Citomegalovirus/virologia , Células Epiteliais/metabolismo , Glicoconjugados/metabolismo , Histocitoquímica , Infecções por Pasteurella/metabolismo , Infecções por Pasteurella/microbiologia , Rinite Atrófica/metabolismo , Rinite Atrófica/microbiologia , Suínos , Doenças dos Suínos/microbiologiaRESUMO
This study was carried out to investigate the effect of infection with Campylobacter fetus subsp. venerealis (Cfv) on the pattern of lectin binding in the uterus and oviduct of heifers. Cfv persistence was demonstrated by bacterial isolation and immunofluorescence. Infected animals showed variations in the lectin binding pattern when compared with control animals. Cfv-infected heifers showed an increased expression of galactose and N-acetyl-galactosamine in the endometrial glands (PNA and SBA binding, respectively). The oviductal epithelium of infected heifers was strongly positive for Con A, which indicated the presence of alpha-D-mannose and alpha-D-glucose. The results of this study showed that Cfv-infection modifies the lectin binding pattern in the reproductive system of heifers. Modifications in glycoconjugates may be involved in failures of fertility and/or implantation.
Assuntos
Infecções por Campylobacter/veterinária , Campylobacter fetus , Doenças dos Bovinos/microbiologia , Tubas Uterinas/metabolismo , Lectinas/metabolismo , Útero/metabolismo , Animais , Infecções por Campylobacter/metabolismo , Infecções por Campylobacter/patologia , Bovinos , Doenças dos Bovinos/metabolismo , Doenças dos Bovinos/patologia , Tubas Uterinas/patologia , Feminino , Útero/patologiaRESUMO
Histochemical studies on tissue sections showed alterations of lectin-binding reactivities in the epididymis, seminal vesicle and ampulla of Brucella ovis-infected rams. These modifications in the carbohydrate composition of organs participating in maturation, transport, and storage of spermatozoa, could be involved in the impaired fertility observed in this disease.
Assuntos
Brucella/isolamento & purificação , Brucelose/veterinária , Epididimo/microbiologia , Glândulas Seminais/microbiologia , Doenças dos Ovinos/microbiologia , Doenças dos Ovinos/patologia , Ducto Deferente/microbiologia , Animais , Brucelose/diagnóstico , Brucelose/patologia , Carboidratos/análise , Epididimo/química , Histocitoquímica/métodos , Lectinas/análise , Masculino , Glândulas Seminais/química , Ovinos , Doenças dos Ovinos/diagnóstico , Ducto Deferente/químicaRESUMO
Immunocharacterization of intermediate filament proteins and lectin-binding studies were carried out in canine transmissible venereal tumour (TVT), a unique neoplasia sharing some epidemiological features with Kaposi's sarcoma in humans. Neoplastic cells express vimentin, but neither cytokeratin nor desmin. Regarding lectins, TVT cells express receptors for Triticum vulgaris (WGA), Concanavalia ensiformis (Con A) and Ricinus communis I (RCA-I). They appear to be negative for Ulex europaeus-I (UEA-I), Arachis hypogaea (PNA), Glycine maximus (SBA) and Dolichos biflorus (DBA).
Assuntos
Doenças do Cão/patologia , Proteínas de Filamentos Intermediários/análise , Neoplasias Penianas/veterinária , Neoplasias Vaginais/veterinária , Animais , Cães , Feminino , Histocitoquímica/métodos , Lectinas , Masculino , Neoplasias Penianas/patologia , Neoplasias Vaginais/patologiaRESUMO
Double immunoenzymatic labelling made possible the simultaneous staining of two antigens with a mixture of polyclonal and monoclonal commercial antibodies. Immunocharacterization of intermediate filament proteins was found to be an accurate indicator of histogenesis in urinary bladder tumours of cattle.
Assuntos
Doenças dos Bovinos/metabolismo , Técnicas Imunoenzimáticas/veterinária , Filamentos Intermediários/química , Neoplasias da Bexiga Urinária/veterinária , Animais , Anticorpos Monoclonais , Bovinos , Desmina/análise , Queratinas/análise , Neoplasias da Bexiga Urinária/química , Neoplasias da Bexiga Urinária/patologia , Vimentina/análiseRESUMO
The peroxidase-antiperoxidase (PAP), streptavidin-biotin (SB), and avidin-biotin-complex (ABC) techniques have been evaluated for the visualization of Mycobacterium paratuberculosis (Mp) in formalin-fixed, paraffin-embedded bovine tissues. The used immunoperoxidase techniques were comparatively better than the Ziehl-Neelsen stain, specially for the demonstration of small number of mycobacteria in tissue sections.