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1.
Viruses ; 15(4)2023 04 21.
Artigo em Inglês | MEDLINE | ID: mdl-37113004

RESUMO

Human bocavirus (HBoV) is an emerging virus detected around the world that may be associated with cases of acute gastroenteritis (AGE). However, its contribution to AGE has not been elucidated. This study aimed to describe the frequency, clinical features, and HBoV species circulation in children up to 5 years with or without AGE symptoms in Acre, Northern Brazil. A total of 480 stool samples were collected between January and December 2012. Fecal samples were used for extraction, nested PCR amplification, and sequencing for genotyping. Statistical analysis was applied to verify the association between epidemiological and clinical characteristics. Overall, HBoV-positivity was 10% (48/480), with HBoV-positive rates of 8.4% (19/226) and 11.4% (29/254) recorded in diarrheic and non-diarrheic children, respectively. The most affected children were in the age group ranging between 7 and 24 months (50%). HBoV infection was more frequent in children who live in urban areas (85.4%), use water from public networks (56.2%), and live with adequate sewage facilities (50%). Co-detection with other enteric viruses was 16.7% (8/48) and the most prevalent coinfection was RVA+ HBoV (50%, 4/8). HBoV-1 was the most frequent species detected in diarrheic and non-diarrheic children, responsible for 43.8% (21/48) of cases, followed by HBoV-3 (29.2%, 14/48) and HBoV-2 (25%, 12/48). In this study, HBoV infection was not always associated with AGE, as most HBoV cases belonged to the non-diarrheal group. Future studies are warranted in order to determine the role of HBoV in causing acute diarrhea disease.


Assuntos
Bocavirus , Gastroenterite , Bocavirus Humano , Infecções por Parvoviridae , Infecções Respiratórias , Humanos , Criança , Lactente , Pré-Escolar , Bocavirus Humano/genética , Brasil/epidemiologia , Infecções por Parvoviridae/epidemiologia , Gastroenterite/epidemiologia , Diarreia/epidemiologia , Fezes , Doença Aguda
2.
Viruses ; 14(10)2022 09 22.
Artigo em Inglês | MEDLINE | ID: mdl-36298656

RESUMO

The rapid and disorderly urbanization in the Amazon has resulted in the insertion of forest fragments into cities, causing the circulation of arboviruses, which can involve hematophagous arthropods and free-ranging birds in the transmission cycles in urban environments. This study aimed to evaluate the circulation of arboviruses in free-ranging birds and hematophagous arthropods captured in an Environmental Protection Area in the Belem metropolitan area, Brazil. Birds were captured using mist nets, and hematophagous arthropods were collected using a human protected attraction technique and light traps. The birds' sera were subjected to a hemagglutination inhibition test to detect antibodies against 29 arbovirus antigens. Arthropod macerates were inoculated into C6/36 and VERO cell cultures to attempt viral isolation and were tested using indirect immunofluorescence, subsequent genetic sequencing and submitted for phylogenetic analysis. Four bird sera were positive for arbovirus, and one batch of Psorophora ferox was positive for Flavivirus on viral isolation and indirect immunofluorescence. In addition, the Ilheus virus was detected in the sequencing and phylogenetic analysis. The presence of antibodies in sera from free-ranging birds and the isolation of Ilheus virus in Psorophora ferox indicate the circulation of arboviruses in forest remnants in the urban center of Belem.


Assuntos
Infecções por Arbovirus , Arbovírus , Artrópodes , Culicidae , Animais , Humanos , Conservação dos Recursos Naturais , Nematóceros , Filogenia , Aves , Florestas , Ecossistema , Infecções por Arbovirus/veterinária
3.
J Med Virol ; 94(2): 610-615, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34427937

RESUMO

Rotaviruses belonging to species A (RVA) remain among the most common causes of severe gastroenteritis in children aged <5 years, leading to substantial morbidity and mortality worldwide. Genome reassortment events between two human strains or human and animal strains represent one of the mechanisms which appear to generate the broad genetic variability of circulating. According to a nucleotide, sequence-based classification system, RVA strains are currently classified into three genotype constellations including Wa-like (genogroup I), DS-1-like (genogroup II), and AU-like (genogroup III). The present study reports the detection of an unusual RVA G4P[6] strain (coded as strain HSE005), which might have originated from a natural reassortment event between human and animal RVA strains. Molecular characterization of this isolate showed that it belonged to genogroup II, genotype G4P[6]. In addition, two genes (VP3 and NSP4) of this strain denoted evidence of reassortment events involving strains of distinct zoonotic evolutionary origins. Therefore, we propose that a new G4P[6] strain was identified, highlighting a possible first zoonotic transmission including a reassortment event that involved the VP3 gene.


Assuntos
Gastroenterite/virologia , Genótipo , Rotavirus/genética , Brasil , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Lactente , RNA Viral , Rotavirus/classificação , Rotavirus/isolamento & purificação
4.
PLoS Negl Trop Dis ; 15(9): e0008792, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34506499

RESUMO

The present study reports the occurrence of rotavirus A (RVA), rotavirus D (RVD), rotavirus F (RVF), rotavirus G (RVG), and picobirnavirus (PBV) in fecal specimens of wild (n = 22), and exotic birds (n = 1) from different cities of Pará state. These animals were hospitalized at Veterinary Hospital of the Federal University of Pará, Brazil, in a period from January 2018 to June 2019. The animals exhibited different clinical signs, such as diarrhea, malnutrition, dehydration, and fractures. The results showed 39.1% (9/23) of positivity for RVA by RT-qPCR. Among these, one sample (1/9) for the NSP3 gene of T2 genotype was characterized. About 88.9% (8/9) for the VP7 gene belonging to G1, G3 equine like and G6 genotypes, and 55.5% (5/9) for the VP4 gene of P[2] genotype were obtained. In the current study, approximately 4.5% of the samples (1/23) revealed coinfection for the RVA, RVD and RVF groups. Furthermore, picobirnavirus (PBV) was detected in one of the 23 samples tested, and was classified in the Genogroup I. The findings represent the first report of RVA, RVD, RVF, RVG, and PBV genotypes in wild birds in Brazil, and due to wide distribution it can implies potential impacts of RVs, and PBVs on avian health, and other animals contributing to construction of new knowledge, and care perspectives.


Assuntos
Doenças das Aves/virologia , Picobirnavirus/isolamento & purificação , Infecções por Vírus de RNA/veterinária , Infecções por Rotavirus/veterinária , Rotavirus/isolamento & purificação , Animais , Animais Selvagens , Doenças das Aves/epidemiologia , Aves , Brasil/epidemiologia , Fezes/virologia , Genótipo , Filogenia , Picobirnavirus/genética , Infecções por Vírus de RNA/epidemiologia , Infecções por Vírus de RNA/virologia , Rotavirus/genética , Infecções por Rotavirus/epidemiologia , Infecções por Rotavirus/virologia
5.
Artigo em Inglês | MEDLINE | ID: mdl-30863824

RESUMO

We identified a strain of Alphacoronavirus 1, FCoV-SB22, from a pool of fecal samples from domestic cats from a rural settlement in the municipality of Santa Bárbara, Pará, Brazil. The nucleotide identity with feline coronavirus was 91.5%. The present study reports the first complete genome sequence of a feline coronavirus from Brazil.

7.
Artigo em Inglês | MEDLINE | ID: mdl-30637382

RESUMO

A proposed new strain of canine Kobuvirus was identified in fecal samples of domestic dogs from a rural community located in the municipality of Peixe-Boi, Pará, Brazil. The nucleotide identity was 92.3% similar to other representatives of the family Picornaviridae, genus Kobuvirus, and species Aichivirus A, which suggests that this is possibly a new strain within this species.

8.
PLoS One ; 13(12): e0209005, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30562373

RESUMO

Acute gastroenteritis is one of the main causes of mortality in humans and young animals. Domestic and mainly wild animals such as bats, small rodents and birds are highly diversified animals in relation to their habitats and ecological niches and are widely distributed geographically in environments of forest fragmentation in some areas of the Amazon, being considered important sources for viruses that affect humans and other animals. Due to the anthropical activities, these animals changed their natural habitat and adapted to urbanized environments, thus representing risks to human and animal health. Although the knowledge of the global diversity of enteric viruses is scarce, there are reports demonstrating the detection of rotavirus in domestic animals and animals of productive systems, such as bovines and pigs. The present study investigated the prevalence of Rotavirus A in 648 fecal samples of different animal species from the northeastern mesoregion of the state of Pará, Brazil, which is characterized as an urbanized area with forest fragments. The fecal specimens were collected from October 2014 to April 2016 and subjected to a Qualitative Real-Time Polymerase Chain Reaction (RT-qPCR), using the NSP3 gene as a target. It was observed that 27.5% (178/648) of the samples presented positive results for RVA, with 178 samples distributed in birds (23.6%), canines (21.35%), chiropterans (17.98%), bovines (14.6%), horses (8.43%), small rodents (6.74%), pigs (3.93%) and felines (3.37%), demonstrating the circulation of RVA in domestic animals and suggesting that such proximity could cause transmissions between different species and the occurrence of rearrangements in the genome of RVA as already described in the literature, associated to the traces of environmental degradation in the studied areas.


Assuntos
Animais Domésticos/virologia , Animais Selvagens/virologia , Florestas , Infecções por Rotavirus/veterinária , Rotavirus , Animais , Brasil , Cidades , Fezes/virologia , Infecções por Rotavirus/epidemiologia , Urbanização
9.
Genome Announc ; 6(25)2018 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-29930038

RESUMO

Our results show the first full-genome characterization of avian nephritis virus 2 recovered from stools of broiler chickens at a commercial farm located in Benevides, Pará, Brazil. Nucleotide analyses of whole-genome sequences showed the isolate to be a strain of Avastrovirus 2 in the family Astroviridae.

10.
Genome Announc ; 6(12)2018 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-29567730

RESUMO

A new strain of avian picornavirus was identified in fecal samples from broiler chickens in a commercial farm in the municipality of Benevides, Pará, Brazil. Genomic analysis showed it to have a nucleotide identity of 78.4% with the family Picornaviridae, genus Avisivirus, and species Avisivirus A, suggesting that this is a possible new strain within this species.

11.
Mem Inst Oswaldo Cruz ; 112(6): 391-395, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28591398

RESUMO

BACKGROUND: Norovirus (NoV) is a major cause of acute gastroenteritis (AGE) worldwide, especially in children under five years. Studies involving the detection and molecular characterisation of NoV have been performed in Brazil, demonstrating its importance as an etiological agent of AGE. OBJECTIVES: The objectives of this study were to investigate the frequency of human NoV and to genotype the strains isolated from 0-14-year-old patients of AGE in Manaus, Brazil, over a period of two years. METHODS: A total of 426 faecal samples were collected between January 2010 and December 2011. All samples were tested for the presence of NoV antigens using a commercial enzyme immunoassay kit. RNA was extracted from all faecal suspensions and reverse transcription-polymerase chain reaction (RT-PCR) for the NoV-polymerase partial region was performed as a trial test. Positive samples were then subjected to PCR with specific primers for partial capsid genes, which were then sequenced. FINDINGS: NoV was detected in 150 (35.2%) faecal samples, for at least one of the two techniques used. NoV was detected in children from all age groups, with the highest positivity observed among the group of 1-2 years old. Clinically, fever was verified in 43% of the positive cases and 46.3% of the negative cases, and vomiting was observed in 75.8% and 70.8% cases in these groups, respectively. Monthly distribution showed that the highest positivity was observed in January 2010 (81.2%), followed by February and April 2010 and March 2011, when the positivity rate reached almost 50%. Phylogenetic analyses performed with 65 positive strains demonstrated that 58 (89.2%) cases of NoV belonged to genotype GII.4, five (7.7%) to GII.6, and one (1.5%) each to GII.7 and GII.3. MAIN CONCLUSIONS: This research revealed a high circulation of NoV GII.4 in Manaus and contributed to the understanding of the importance of this virus in the aetiology of AGE cases, especially in a region with such few studies available.


Assuntos
Infecções por Caliciviridae/virologia , Gastroenterite/virologia , Norovirus/genética , Norovirus/isolamento & purificação , Doença Aguda , Adolescente , Brasil/epidemiologia , Infecções por Caliciviridae/epidemiologia , Criança , Pré-Escolar , Fezes/virologia , Feminino , Gastroenterite/epidemiologia , Variação Genética , Genótipo , Humanos , Lactente , Recém-Nascido , Masculino , Prevalência , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa
12.
J Water Health ; 15(1): 163-174, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28151449

RESUMO

This study investigated the presence of norovirus (NoV) GI and GII in environmental samples from the northern region of Brazil. Water samples were collected monthly (November 2008/October 2010) from different sources and sewage and concentrated by the adsorption-elution method. The NoV investigation used molecular methods followed by sequencing reactions. The general positivity for NoV was 33.9% (57/168). Considering the results obtained only in the semi-nested RT-PCR (reverse transcription polymerase chain reaction) and only in the TaqMan® real-time PCR, the rates were 26.8% (45/168) and 27.4% (46/168), respectively, being for NoV GI 22.2% (10/45) and 19.6% (9/46); for GII 17.8% (8/45) and 15.2% (7/46); and for GI + GII 60% (27/45) and 65.2% (30/46), respectively. Different GI (GI.1, GI.4, GI.7 and GI.8) and GII (GII.4, GII.6, GII.9, GII.12 and GII.14) genotypes were detected. These results demonstrated the NoV was disseminated in the waters of Belém city due to a lack of sanitation that allowed the discharge of contaminated effluents into these aquatic ecosystems.


Assuntos
Monitoramento Ambiental/métodos , Água Doce/microbiologia , Norovirus/isolamento & purificação , Esgotos/microbiologia , Brasil , Genótipo , Norovirus/genética , Fases de Leitura Aberta , Filogenia , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de RNA
13.
J Gen Virol ; 97(12): 3131-3138, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27902376

RESUMO

Genotype G3P[8] of rotavirus A (RVA) is detected worldwide, usually associated with Wa-like constellation and exhibiting a long RNA migration pattern. More recently, a novel inter-genogroup, G3P[8] reassortant variant with a short electropherotype, has emerged in Asia, Oceania and Europe, denoting an overall potential of unusual rotavirus strains. During a RVA surveillance in Brazil, G3P[8] strains were found displaying a short electropherotype pattern, which had not been detected before in this region. This study aims to characterize the complete genome of 10 G3P[8] strains detected in the northern region of Brazil. All G3P[8] samples were subjected to partial sequencing, and the whole-genome phylogenetic analysis demonstrated that all strains possessed I2-R2-C2-M2-A2-N1-T2-E2-H2 genotype background, representing reassortants with an equine-like G3 VP7 and amino acid changes in VP4 and VP7 antigenic regions as compared to vaccine strains. Phylogenetic analysis demonstrated high nucleotide identity in almost all RNA segments of G3P[8] DS-1 samples detected in Asia, Oceania and Europe as well as G3P[4] strains in Japan. This study reports a novel, equine-like G3P[8] strain circulating in Brazil and isolated from children hospitalized for severe gastroenteritis, and highlights the complex dynamics of RVA molecular epidemiology. Our findings point to a novel RVA strain emerging in this region, and studies should be done to detect whether this may represent a challenge to current vaccine strategies.


Assuntos
Gastroenterite/virologia , Infecções por Rotavirus/virologia , Rotavirus/isolamento & purificação , Doença Aguda/epidemiologia , Sequência de Aminoácidos , Brasil/epidemiologia , Gastroenterite/epidemiologia , Genoma Viral , Genótipo , Humanos , Filogenia , Rotavirus/química , Rotavirus/classificação , Rotavirus/genética , Infecções por Rotavirus/epidemiologia , Alinhamento de Sequência , Proteínas Virais/química , Proteínas Virais/genética
14.
Infect Genet Evol ; 39: 365-371, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26861619

RESUMO

Norovirus (NoV) is responsible for outbreaks and sporadic cases of nonbacterial acute gastroenteritis in humans worldwide. The virus consists of small round particles containing a single-stranded RNA genome that is divided into three Open Reading Frames. NoV evolves via mechanisms of antigenic drift and recombination, which lead to the emergence of new strains that are capable of causing global epidemics. Recombination usually occurs in the ORF1/ORF2 overlapping region and generates strains with different genotypes in the polymerase and capsid region. The primary objective of this study was to analyze recombination in positive-NoV samples. Specimens were collected during 2011, 2012 and 2014, from children under two years of age presenting gastrointestinal symptoms such as vomiting and diarrhea. The partial polymerase (B region), capsid (D region) genes and the ORF1-ORF2 overlap regions were sequenced in each sample. The recombinant analyses were performed in the Simplot software v.3.5.1 and RDP4 Beta v. 4.6 program. These analyses showed that GII.Pg/GII.1, GII.P7/GII.6, and GII.P22/GII.5 were recombinant strains. To our knowledge, this is the first time that the GII.P22/GII.5 and GII.Pg/GII.1 strains were described in South America and the GII.P7/GII.6 was detected in Northern of Brazil.


Assuntos
Infecções por Caliciviridae/epidemiologia , Infecções por Caliciviridae/virologia , Gastroenterite/epidemiologia , Gastroenterite/virologia , Norovirus/classificação , Norovirus/genética , Recombinação Genética , Brasil/epidemiologia , Biologia Computacional/métodos , Genótipo , Humanos , Fases de Leitura Aberta , Filogenia
15.
Food Environ Virol ; 8(1): 101-4, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26538419

RESUMO

Noroviruses are the major cause of non-bacterial acute gastroenteritis outbreaks in humans, with few reports about the occurrence of the norovirus GIV strain. We investigated the presence of norovirus GIV in surface water (river, bay, and stream) and untreated sewage, and we determined a positivity rate of 9.4% (9/96). The strains genotyped were GIV.1. To our knowledge, this is the first report of GIV in Brazil.


Assuntos
Norovirus/isolamento & purificação , Rios/virologia , Esgotos/virologia , Brasil , Infecções por Caliciviridae/virologia , Gastroenterite/virologia , Genótipo , Humanos , Norovirus/classificação , Norovirus/genética
16.
J Med Virol ; 88(4): 728-33, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26369400

RESUMO

Group C rotavirus (RVC) is potentially an important pathogen associated with acute gastroenteritis (AG), especially in outbreaks. This study aims to detect and molecularly characterize RVC in hospitalized children with AG in Belém, Brazil. From May 2008 to April 2011, 279 stools were subjected to reverse-transcription polymerase chain reaction targeting VP7, VP6, VP4, and NSP4 genes. RVC positivity rate was 2.1% (6/279) and phylogenetic analysis of positive samples yields genotype G4-P[2]-I2-E2. No evidence of zoonotic transmission and VP7 gene demonstrated close relationship with Asian strains. RVC surveillance is worth to expand information on evolutionary and epidemiological features of this virus.


Assuntos
Gastroenterite/virologia , Variação Genética , Genótipo , Filogenia , Infecções por Rotavirus/virologia , Rotavirus/classificação , Rotavirus/genética , Brasil/epidemiologia , Criança Hospitalizada , Pré-Escolar , Fezes/virologia , Feminino , Gastroenterite/epidemiologia , Humanos , Lactente , Masculino , Epidemiologia Molecular , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Rotavirus/isolamento & purificação , Infecções por Rotavirus/epidemiologia , Proteínas não Estruturais Virais/genética , Proteínas Estruturais Virais/genética
17.
Rev Inst Med Trop Sao Paulo ; 57(1): 27-32, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25651323

RESUMO

INTRODUCTION: The objective of this study was to determine the prevalence and etiological profile of enteropathogens in children from a daycare center. METHODS: From October 2010 to February 2011 stool samples from 100 children enrolled in a government daycare center in the municipality of São José do Rio Preto, in the state of São Paulo, were collected and analyzed. RESULTS: A total of 246 bacteria were isolated in 99% of the fecal samples; 129 were in the diarrheal group and 117 in the non-diarrheal group. Seventy-three strains of Escherichia coli were isolated, 19 of Enterobacter, one of Alcaligenes and one of Proteus. There were 14 cases of mixed colonization with Enterobacter and E. coli. Norovirus and Astrovirus were detected in children with clinical signs suggestive of diarrhea. These viruses were detected exclusively among children residing in urban areas. All fecal samples were negative for the presence of the rotavirus species A and C. The presence of Giardia lamblia, Entamoeba coli, Endolimax nana and hookworm was observed. A significant association was found between food consumption outside home and daycare center and the presence of intestinal parasites. CONCLUSIONS: For children of this daycare center, intestinal infection due to pathogens does not seem to have contributed to the occurrence of diarrhea or other intestinal symptoms. The observed differences may be due to the wide diversity of geographical, social and economic characteristics and the climate of Brazil, all of which have been reported as critical factors in the modulation of the frequency of different enteropathogens.


Assuntos
Fezes , Enteropatias/microbiologia , Enteropatias/parasitologia , Brasil/epidemiologia , Creches , Pré-Escolar , Diarreia/epidemiologia , Diarreia/microbiologia , Diarreia/parasitologia , Fezes/microbiologia , Fezes/parasitologia , Fezes/virologia , Humanos , Enteropatias/virologia , Enteropatias Parasitárias/etiologia , Fatores Socioeconômicos , População Urbana
18.
Rev. Inst. Med. Trop. Säo Paulo ; Rev. Inst. Med. Trop. Säo Paulo;57(1): 27-32, Jan-Feb/2015. tab
Artigo em Inglês | LILACS | ID: lil-736358

RESUMO

Introduction: The objective of this study was to determine the prevalence and etiological profile of enteropathogens in children from a daycare center. Methods: From October 2010 to February 2011 stool samples from 100 children enrolled in a government daycare center in the municipality of São José do Rio Preto, in the state of São Paulo, were collected and analyzed. Results: A total of 246 bacteria were isolated in 99% of the fecal samples; 129 were in the diarrheal group and 117 in the non-diarrheal group. Seventy-three strains of Escherichia coli were isolated, 19 of Enterobacter, one of Alcaligenes and one of Proteus. There were 14 cases of mixed colonization with Enterobacter and E. coli. Norovirus and Astrovirus were detected in children with clinical signs suggestive of diarrhea. These viruses were detected exclusively among children residing in urban areas. All fecal samples were negative for the presence of the rotavirus species A and C. The presence of Giardia lamblia, Entamoeba coli, Endolimax nana and hookworm was observed. A significant association was found between food consumption outside home and daycare center and the presence of intestinal parasites. Conclusions: For children of this daycare center, intestinal infection due to pathogens does not seem to have contributed to the occurrence of diarrhea or other intestinal symptoms. The observed differences may be due to the wide diversity of geographical, social and economic characteristics and the climate of Brazil, all of which have been reported as critical factors in the modulation of the frequency of different enteropathogens.


Introdução: O objetivo deste estudo foi determinar a prevalência e o perfil etiológico de enteropatógenos em crianças de uma creche. Métodos: No período de outubro de 2010 a fevereiro de 2011 foram coletadas e analisadas amostras de fezes de 100 crianças matriculadas em creche do governo no município de São José do Rio Preto, Estado de São Paulo. Resultados: Um total de 246 bactérias foram isoladas em 99% das amostras de fezes; 129 eram diarreicas e 117 não-diarreicas. Foram isoladas setenta e três cepas de Escherichia coli, 19 de Enterobacter, uma de Alcaligenes e uma de Proteus. Foram detectados 14 casos de colonização mista com Enterobacter e de E. coli. Norovírus e Astrovirus foram detectados em crianças com sinais clínicos sugestivos de diarréia. Estes vírus foram detectados exclusivamente entre as crianças residentes em áreas urbanas. Todas as amostras fecais foram negativas para a presença das espécies de rotavírus A e C. Foi observada a presença de Giardia lamblia, Entamoeba coli, Endolimax nana e ancilostomídeos. Foi encontrada associação significativa entre o consumo de alimentos fora do centro da casa e creche e a presença de parasitos intestinais. Conclusões: Para as crianças desta creche, a infecção intestinal por patógenos não parece ter contribuido para a ocorrência de diarreia ou outros sintomas intestinais. As diferenças observadas podem ser atribuídas à grande diversidade de características geográficas, sociais e econômicas e o clima do Brasil, as quais tem sido relatadas como fatores críticos para a modulação da frequência de diferentes enteropatógenos.


Assuntos
Pré-Escolar , Humanos , Fezes , Enteropatias/microbiologia , Enteropatias/parasitologia , Brasil/epidemiologia , Creches , Diarreia/epidemiologia , Diarreia/microbiologia , Diarreia/parasitologia , Fezes/microbiologia , Fezes/parasitologia , Fezes/virologia , Enteropatias Parasitárias/etiologia , Enteropatias/virologia , Fatores Socioeconômicos , População Urbana
19.
Avian Pathol ; 43(3): 238-43, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24875189

RESUMO

Rotaviruses infect humans and animals and are classified into eight groups (A to H). Group D rotavirus (RVD) has been described in birds, although relatively few reports are available. The present study focused on RVD, including epidemiological and molecular aspects of samples collected from broiler chickens in the state of Pará, Brazil. A total of 85 faecal samples were collected between 2008 and 2011 from 37 chicken farms located in eight different municipalities. The viral double-stranded RNA was extracted from faecal suspensions and analysed using polyacrylamide gel electrophoresis (PAGE), followed by reverse transcriptase-polymerase chain reaction (RT-PCR) and nucleotide sequencing of the VP6 and VP7 genes. Comparing the positive results, 16.5% (14/85) were obtained by PAGE and 35.3% (30/85) by RT-PCR. Samples from seven of eight municipalities were positive for RVD and infections were recorded in 17 (45.9%) of 37 chicken farms. The RVD infection rate was significantly higher in the 16-day to 30-day age group (62.2%; 23/37) compared with other ages. No consistent relationship was found between the infection rate and either the population density in poultry houses or the climatic conditions. The nucleotide sequences of the VP6 gene were 89.9 to 90.9% similar to the prototype strain 05V0049 and were 88.3 to 100% similar among themselves; VP7 gene nucleotide sequences were 84.3 to 85.4% similar to the prototype strain 05V0049 and 93.8 to 100% similar among themselves. Overall, this study provides new insights into the epidemiology and genome characterization of group D rotaviruses.


Assuntos
Antígenos Virais/genética , Proteínas do Capsídeo/genética , Galinhas/virologia , Genoma Viral/genética , Infecções por Rotavirus/epidemiologia , Rotavirus/isolamento & purificação , Sequência de Aminoácidos , Animais , Sequência de Bases , Brasil/epidemiologia , Fezes/virologia , Dados de Sequência Molecular , Filogenia , RNA de Cadeia Dupla/genética , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Rotavirus/genética , Infecções por Rotavirus/virologia , Alinhamento de Sequência/veterinária , Análise de Sequência de DNA/veterinária
20.
J Med Virol ; 86(6): 1065-72, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24136444

RESUMO

Rotavirus A (RVA) is the most common cause of severe acute gastroenteritis in infants and young children worldwide, causing 453,000 deaths annually. In Brazil, the most frequent genotype identified was G1 during almost three decades in the pre-vaccination period; however, after anti-rotavirus vaccine introduction, there was a predominance of G2 genotype. The aim of this study was to determine the G and P genotypes of rotaviruses isolated from children under 5 years of age with acute gastroenteritis in the Northern region of Brazil, and discuss the emergence of G3P[6] genotype. A total of 783 stool specimens were obtained between January 2011 and March 2012. RVA antigen was detected in 33% (272/783) of samples using a commercial enzyme-linked immunosorbent assay and type-specificity was determined by reverse-transcription polymerase chain reaction. The most common binary combination was G2P[4], representing 41% of cases, followed by G3P[6] (15%), G1P[8] (8%), G3P[8] (4%), G9P[8] (3%), and G12P[6] (2%). G3P[6] strains were analyzed further and phylogenetic analysis of VP7 gene showed that G3 strains clustered into lineage I and showed a high degree of amino acid identity with vaccine strain RV3 (95.1-95.6%). For VP4 sequences, G3P[6] clustered into lineage Ia. It was demonstrated by the first time the emergence of unusual genotype G3P[6] in the Amazon region of Brazil. This genotype shares neither VP7 nor VP4 specificity with the used vaccine and may represent a challenge to vaccination strategies. A continuous monitoring of circulating strains is therefore needed during the post-vaccine era in Brazil.


Assuntos
Infecções por Rotavirus/epidemiologia , Infecções por Rotavirus/virologia , Vacinas contra Rotavirus/administração & dosagem , Rotavirus/classificação , Rotavirus/genética , Antígenos Virais/análise , Brasil/epidemiologia , Proteínas do Capsídeo , Criança , Pré-Escolar , Análise por Conglomerados , Ensaio de Imunoadsorção Enzimática , Fezes/virologia , Feminino , Genótipo , Humanos , Lactente , Recém-Nascido , Masculino , Epidemiologia Molecular , Dados de Sequência Molecular , Filogenia , Prevalência , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Rotavirus/isolamento & purificação , Infecções por Rotavirus/prevenção & controle , Análise de Sequência de DNA
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