RESUMO
Listeria monocytogenes is a foodborne pathogen that causes listeriosis and can be a problem in areas where meat products are sold at unregulated storage temperatures. In this work, the prevalence of L. monocytogenes was determined in the five most widely traded meat products in the province of Quevedo (Ecuador): bacon, "chorizo paisa", grilled hamburger meat, mortadella, and salami. A total of 1000 samples of these products were analyzed in two seasons of the year (dry season/rainy season). All L. monocytogenes isolates were confirmed by PCR with primers designed for the iap gene. Furthermore, the positive samples were quantified for L. monocytogenes. Of the 1000 meat products analyzed, 163 were positive for L. monocytogenes (16.3%). The prevalence of L. monocytogenes in the two seasons in different meat products was as follows: 22.5% in mortadella, 19% in hamburger meat, 15% in bacon, 14.5% in chorizo paisa and 10.5% in salami. In addition, the concentration of L. monocytogenes in most of the positive samples was in the range of 4-6 log CFU/g or even higher. The results show the need for improvements in the hygienic measures and meat storage temperatures in Quevedo (Ecuador) to avoid risks of foodborne listeriosis.
RESUMO
BACKGROUND: Listeria monocytogenes is a widespread common contaminant in food production facilities during preparation, storage, and distribution, and minimally processed ready-to-eat products are considered at high risk of contamination by this bacterium. Increased antibiotic resistance has led researchers to search for plant-based natural alternatives to control pathogenic microorganisms. Among these products, essential oils and plant extracts have previously shown antimicrobial activity and are possible alternatives to manage food pathogens. In this study, commercial essential oils (cinnamon, clove, oregano, ginger, and thyme) and plant extracts (pomegranate, acorn, olive, strawberry tree, and dog rose) were tested against L. monocytogenes in a dry-cured ham-based model. RESULTS: Essential oils and plant extracts were screened by agar diffusion and minimum inhibitory concentration for anti-L. monocytogenes activity. Cinnamon, pomegranate, and strawberry trees returned the strongest results and were therefore evaluated in a dry-cured ham-based medium assay with water activity of 0.93 or 0.95. The 10% essential oil of cinnamon was capable of completely inhibiting bacterial growth, while strawberry tree and pomegranate extract also showed antilisterial activity (P > 0.05). Water activity influenced the bacterial count of L. monocytogenes in a dry-cured ham-based medium. CONCLUSIONS: There was a reduction in L. monocytogenes with the application of cinnamon essential oil but, because of the negative sensory impact of this particular compound in meat products, we suggest the use of pomegranate or strawberry tree for the biocontrol of Listeria in ready-to-eat products. © 2021 Society of Chemical Industry.
Assuntos
Anti-Infecciosos , Conservação de Alimentos , Listeria monocytogenes , Óleos Voláteis , Carne de Porco , Animais , Anti-Infecciosos/farmacologia , Contagem de Colônia Microbiana , Contaminação de Alimentos , Microbiologia de Alimentos , Listeria monocytogenes/efeitos dos fármacos , Produtos da Carne , Óleos Voláteis/farmacologia , Extratos Vegetais/farmacologiaRESUMO
Atlantia is described as a new genus pertaining to the family Dendrophylliidae (Anthozoa, Scleractinia) based on specimens from Cape Verde, eastern Atlantic. This taxon was first recognized as Enallopsammia micranthus and later described as a new species, Tubastraea caboverdiana, which then changed the status of the genus Tubastraea as native to the Atlantic Ocean. Here, based on morphological and molecular analyses, we compare fresh material of T. caboverdiana to other dendrophylliid genera and describe it as a new genus named Atlantia in order to better accommodate this species. Evolutionary reconstruction based on two mitochondrial and one nuclear marker for 67 dendrophylliids and one poritid species recovered A. caboverdiana as an isolated clade not related to Tubastraea and more closely related to Dendrophyllia cornigera and Leptopsammia pruvoti. Atlantia differs from Tubastraea by having a phaceloid to dendroid growth form with new corallites budding at an acute angle from the theca of a parent corallite. The genus also has normally arranged septa (not Portualès Plan), poorly developed columella, and a shallow-water distribution all supporting the classification as a new genus. Our results corroborate the monophyly of the genus Tubastraea and reiterate the Atlantic non-indigenous status for the genus. In the light of the results presented herein, we recommend an extensive review of shallow-water dendrophylliids from the Eastern Atlantic.
RESUMO
BACKGROUND: Although cross-reactivity with other plant-based foods and latex has been described, allergies to potatoes and tomatoes are uncommon. OBJECTIVE: To study the different sensitization patterns in patients who are allergic to potatoes and/or tomatoes. METHODS: Skin prick tests were carried out with fresh foods and extracts, specific IgE determination and allergen detection by SDS-PAGE and IgE-Immunoblotting with both raw and heated potato and tomato extracts. RESULTS: In 10 patients, two thermostable allergens to potato extract were detected; the first one with a molecular weight that is compatible with Sola t 1 (43 kDa, patatin) and the second one with a molecular weight of 14-22 kDa, which could correspond to the allergens Sola t 4 (16 kDa) and Sola t 2 and Sola t 3 (21 kDa); in two patients who are allergic to potatoes and two patients who are allergic to tomatoes, a thermostable allergen that is compatible to Sola I 2 (50 kDa) was detected. The patient had presented oral allergy syndrome with some types of potatoes and showed higher IgE reactivity to two thermostable potato allergens. CONCLUSIONS: The allergen sensitization patterns were similar in all the patients that had been studied, regardless of the symptoms. A new allergen involved in the allergy to solanaceae plants has been detected.
Antecedentes: Aunque se ha descrito reactividad cruzada con alimentos vegetales y látex, la alergia a la papa y al tomate es infrecuente. Objetivo: Estudiar los diferentes patrones de sensibilización en pacientes alérgicos a la patata o tomate. Métodos: Se realizaron pruebas de punción cutánea con extractos y alimentos frescos, determinación de IgE específica y detección de alérgenos mediante SDS-PAGE e IgE-Immunoblotting con extractos de patata y tomate crudos y calientes. Resultados: En 10 pacientes se detectaron alérgenos termoestables a extracto de patata, uno de peso molecular compatible con Sola t 1 (43 kDa, patatina) y otro de 14-22 kDa que podría corresponder a los alérgenos Sola t 4 (16 kDa), Sola t 2 y Sola t 3 (21 kDa); en dos pacientes alérgicos a la patata y dos alérgicos al tomate se detectó un alérgeno termoestable de aproximadamente 42 kDa. En un paciente alérgico al tomate se detectó un alérgeno termoestable compatible con Sola l 2 (50 kDa); había presentado síndrome de alergia oral con algunos tipos de patatas y mostró mayor reactividad IgE a dos alérgenos termoestables de la patata. Conclusiones: Los patrones de sensibilización a los alérgenos fueron similares en los pacientes, independientemente de los síntomas. Se ha detectado un nuevo alérgeno implicado en la alergia a las solanáceas.
Assuntos
Hipersensibilidade Alimentar/imunologia , Solanum lycopersicum/efeitos adversos , Solanum tuberosum/efeitos adversos , Adolescente , Adulto , Alérgenos/análise , Feminino , Hipersensibilidade Alimentar/diagnóstico , Humanos , Solanum lycopersicum/química , Solanum lycopersicum/imunologia , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Testes Cutâneos , Solanum tuberosum/química , Solanum tuberosum/imunologia , Adulto JovemRESUMO
To increase understanding of drug-resistant Vibrio cholerae, we studied selected molecular mechanisms of antimicrobial drug resistance in the 2010 Haiti V. cholerae outbreak strain. Most resistance resulted from acquired genes located on an integrating conjugative element showing high homology to an integrating conjugative element identified in a V. cholerae isolate from India.
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Cólera/epidemiologia , Surtos de Doenças , Farmacorresistência Bacteriana Múltipla , Vibrio cholerae O1/efeitos dos fármacos , Antibacterianos/farmacologia , Farmacorresistência Bacteriana Múltipla/genética , Ordem dos Genes , Genoma Bacteriano , Haiti/epidemiologia , Humanos , Testes de Sensibilidade Microbiana , Filogenia , Vibrio cholerae O1/genética , Vibrio cholerae O1/isolamento & purificaçãoRESUMO
La Fibrosis Quística (FQ), enfermedad congénita y hereditaria cuyas manifestaciones clínicas a nivel del sistema respiratorio y digestivo llevan al paciente a una desnutrición progresiva yrepetidos eventos de manifestaciones de estos dos sistemas con el consecuente ingreso prolongado. Se realizó un estudioexperimental preclínico con el fin de evaluar los resultados del tratamiento homeopático en pacientes portadores de FQ de la provincia de Camagüey atendidos en el Hospital PediátricoProvincial en el período de enero del 2004 a diciembre del 2006. Se constató como resultado global una mejor calidad de vida con disminución de síntomas, aumento de peso, ganancia en calidad de la actividad física y una recuperación psicosocial.(AU)
Assuntos
Humanos , Masculino , Feminino , Lactente , Pré-Escolar , Criança , Adolescente , Fibrose Cística/diagnóstico , Fibrose Cística/terapia , Sintomatologia , Terapêutica Homeopática , Medicamento HomeopáticoRESUMO
La Fibrosis Quística (FQ), enfermedad congénita y hereditaria cuyas manifestaciones clínicas a nivel del sistema respiratorio y digestivo llevan al paciente a una desnutrición progresiva yrepetidos eventos de manifestaciones de estos dos sistemas con el consecuente ingreso prolongado. Se realizó un estudioexperimental preclínico con el fin de evaluar los resultados del tratamiento homeopático en pacientes portadores de FQ de la provincia de Camagüey atendidos en el Hospital PediátricoProvincial en el período de enero del 2004 a diciembre del 2006. Se constató como resultado global una mejor calidad de vida con disminución de síntomas, aumento de peso, ganancia en calidad de la actividad física y una recuperación psicosocial.
Assuntos
Humanos , Masculino , Feminino , Lactente , Pré-Escolar , Criança , Adolescente , Relatos de Casos , Fibrose Cística/diagnóstico , Fibrose Cística/terapia , Terapêutica Homeopática , Sinais e Sintomas , Medicamento HomeopáticoRESUMO
Se presenta el caso de un paciente de 8 anos de edad con fractura desplazada del 1/3 superior de humero izquierdo. Se inicia tratamiento homeopatico con Calcarea carbonica 30c. A los 15 dias se formo un buen callo oseo y en los siguientes 20 dias el humero remodela totalmente.(AU)
Assuntos
Relatos de Casos , Humanos , Masculino , Criança , Consolidação da Fratura , Calo Ósseo , Calcarea Carbonica/uso terapêuticoRESUMO
Se presenta el caso de un paciente de 8 anos de edad con fractura desplazada del 1/3 superior de humero izquierdo. Se inicia tratamiento homeopatico con Calcarea carbonica 30c. A los 15 dias se formo un buen callo oseo y en los siguientes 20 dias el humero remodela totalmente.