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1.
Neural Plast ; 2022: 7432842, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36213614

RESUMO

The dentate gyrus (DG) is the gateway of sensory information arriving from the perforant pathway (PP) to the hippocampus. The adequate integration of incoming information into the DG is paramount in the execution of hippocampal-dependent cognitive functions. An abnormal DG granule cell layer (GCL) widening due to granule cell dispersion has been reported under hyperexcitation conditions in animal models as well as in patients with mesial temporal lobe epilepsy, but also in patients with no apparent relation to epilepsy. Strikingly, it is unclear whether the presence and severity of GCL widening along time affect synaptic processing arising from the PP and alter the performance in hippocampal-mediated behaviors. To evaluate the above, we injected excitotoxic kainic acid (KA) unilaterally into the DG of mice and analyzed the evolution of GCL widening at 10 and 30 days post injection (dpi), while analyzing if KA-induced GCL widening affected in vivo long-term potentiation (LTP) in the PP-DG pathway, as well as the performance in learning and memory through contextual fear conditioning. Our results show that at 10 dpi, when a subtle GCL widening was observed, LTP induction, as well as contextual fear memory, were impaired. However, at 30 dpi when a pronounced increase in GCL widening was found, LTP induction and contextual fear memory were already reestablished. These results highlight the plastic potential of the DG to recover some of its functions despite a major structural alteration such as abnormal GCL widening.


Assuntos
Giro Denteado , Potenciação de Longa Duração , Animais , Cognição , Giro Denteado/metabolismo , Medo , Ácido Caínico/metabolismo , Ácido Caínico/toxicidade , Potenciação de Longa Duração/fisiologia , Plásticos/metabolismo
2.
Mol Ther ; 30(2): 798-815, 2022 02 02.
Artigo em Inglês | MEDLINE | ID: mdl-34563674

RESUMO

Spontaneous recovery after a stroke accounts for a significant part of the neurological recovery in patients. However limited, the spontaneous recovery is mechanistically driven by axonal restorative processes for which several molecular cues have been previously described. We report the acceleration of spontaneous recovery in a preclinical model of ischemia/reperfusion in rats via a single intracerebroventricular administration of extracellular vesicles released from primary cortical astrocytes. We used magnetic resonance imaging and confocal and multiphoton microscopy to correlate the structural remodeling of the corpus callosum and striatocortical circuits with neurological performance during 21 days. We also evaluated the functionality of the corpus callosum by repetitive recordings of compound action potentials to show that the recovery facilitated by astrocytic extracellular vesicles was both anatomical and functional. Our data provide compelling evidence that astrocytes can hasten the basal recovery that naturally occurs post-stroke through the release of cellular mediators contained in extracellular vesicles.


Assuntos
Vesículas Extracelulares , Acidente Vascular Cerebral , Animais , Astrócitos , Axônios , Modelos Animais de Doenças , Humanos , Imageamento por Ressonância Magnética , Ratos , Recuperação de Função Fisiológica/fisiologia , Acidente Vascular Cerebral/patologia
3.
Neurobiol Learn Mem ; 167: 107125, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31770584

RESUMO

Brain-derived neurotrophic factor (BDNF) is an essential product of protein synthesis with a prominent impact on brain signaling and synaptic plasticity. Exogenous application of this neurotrophin is able to induce long-term potentiation (LTP) in several brain structures such as the hippocampus along with increases in gene transcription and translation of proteins involved in functional and structural plasticity. In this regard, our previous studies have demonstrated that acute intrahippocampal administration of BDNF induces long-lasting enhancement of synaptic transmission at the mossy fibers projection (MF) accompanied by a structural reorganization at the CA3 hippocampus area. Thus, considering the non-canonical molecular mechanisms underlying MF-CA3-LTP and the high expression of this neurotrophin in the CA3 area, we wonder whether transcriptional and translational inhibition interferes with the persistence of the MF functional and structural synaptic plasticity elicited by BDNF in adult rats in vivo. Our results show that BDNF is able to induce a lasting potentiation of synaptic efficacy at the MF projection accompanied by a structural reorganization at the CA3 area in an mRNA synthesis and protein translation-independent manner. The present findings support the idea that BDNF is an essential plasticity related product, which is necessary and sufficient to induce and maintain functional and structural synaptic plasticity at the MF-CA3 pathway.


Assuntos
Fator Neurotrófico Derivado do Encéfalo/metabolismo , Região CA3 Hipocampal/metabolismo , Potenciação de Longa Duração , Fibras Musgosas Hipocampais/metabolismo , Transmissão Sináptica , Animais , Fator Neurotrófico Derivado do Encéfalo/administração & dosagem , Região CA3 Hipocampal/fisiologia , Expressão Gênica , Masculino , RNA Mensageiro/metabolismo , Ratos Wistar
4.
Neurobiol Learn Mem ; 142(Pt A): 85-90, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28034786

RESUMO

The history of activity of a given neuron has been proposed to bidirectionally influence its future response to synaptic inputs. In particular, induction of synaptic plasticity expressions such as long-term potentiation (LTP) and long-term depression (LTD) modifies the performance of several behavioral tasks. Our previous studies in the insular cortex (IC), a neocortical region that has been related to acquisition and retention of conditioned taste aversion (CTA), have demonstrated that induction of LTP in the basolateral amygdaloid nucleus (Bla)-IC pathway before CTA training enhances the retention of this task. In addition, we reported that CTA training triggers a persistent impairment in the ability to induce in vivo LTP in the IC. The aim of the present study was to investigate whether LTD can be induced in the Bla-IC projection in vivo, as well as, whether the extinction of CTA is bidirectionally modified by previous synaptic plasticity induction in this pathway. Thus, rats received 900 train pulses (five 250µs pulses at 250Hz) delivered at 1Hz in the Bla-IC projection in order to induce LTD or 10 trains of 100Hz/1s with an intertrain interval of 20s in order to induce LTP. Seven days after surgery, rats were trained in the CTA task including the extinction trials. Our results show that the Bla-IC pathway is able to express in vivo LTD in an N-Methyl-D-aspartate (NMDA) receptor-dependent manner. Induction of LTD in the Bla-IC projection previous to CTA training facilitates the extinction of this task. Conversely, LTP induction enhances CTA retention. The present results show the bidirectional modulation of CTA extinction in response to IC-LTP and LTD, providing evidence of the homeostatic adaptation of taste learning.


Assuntos
Aprendizagem da Esquiva/fisiologia , Córtex Cerebral/fisiologia , Condicionamento Clássico/fisiologia , Extinção Psicológica/fisiologia , Potenciação de Longa Duração/fisiologia , Depressão Sináptica de Longo Prazo/fisiologia , Paladar/fisiologia , Animais , Masculino , Ratos , Ratos Wistar
5.
Behav Brain Res ; 297: 1-4, 2016 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-26433146

RESUMO

Nowadays, it is known that brain derived neurotrophic-factor (BDNF) is a protein critically involved in regulating long-term memory related mechanisms. Previous studies from our group in the insular cortex (IC), a brain structure of the temporal lobe implicated in acquisition, consolidation and retention of conditioned taste aversion (CTA), demonstrated that BDNF is essential for CTA consolidation. Recent studies show that BDNF-TrkB signaling is able to mediate the enhancement of memory. However, whether BDNF into neocortex is able to enhance aversive memories remains unexplored. In the present work, we administrated BDNF in a concentration capable of inducing in vivo neocortical LTP, into the IC immediately after CTA acquisition in two different conditions: a "strong-CTA" induced by 0.2M lithium chloride i.p. as unconditioned stimulus, and a "weak-CTA" induced by 0.1M lithium chloride i.p. Our results show that infusion of BDNF into the IC converts a weak CTA into a strong one, in a TrkB receptor-dependent manner. The present data suggest that BDNF into the adult insular cortex is sufficient to increase an aversive memory-trace.


Assuntos
Aprendizagem da Esquiva/efeitos dos fármacos , Fator Neurotrófico Derivado do Encéfalo/farmacologia , Memória/efeitos dos fármacos , Neocórtex/efeitos dos fármacos , Nootrópicos/farmacologia , Percepção Gustatória/efeitos dos fármacos , Animais , Aprendizagem da Esquiva/fisiologia , Cateteres de Demora , Condicionamento Psicológico/efeitos dos fármacos , Condicionamento Psicológico/fisiologia , Cloreto de Lítio , Masculino , Memória/fisiologia , Microinjeções , Neocórtex/fisiologia , Ratos Wistar , Percepção Gustatória/fisiologia , Privação de Água
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