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ABSTRACT: This study evaluated the effect of maternal protein supplementation during mid or late gestation on energy metabolism of the skeletal muscle of beef calves. Sixteen pregnant cows were divided into 3 groups: CTRL (not supplemented); MID (supplemented from 30 to 180 days of gestation); and LATE (supplemented from 181 to 281 days of gestation). The supplement contained 30% crude protein. Thirty days after birth, blood and muscle samples of the calves were collected for analyses of gene expression, proteins, and metabolites. No differences (P ≥ 0.15) in birth weight, performance at weaning, or muscle expression of the genes evaluated (P ≥ 0.21) were observed. Calves born to CTRL cows had a lower ratio (P = 0.03) of p-AMPK/AMPK protein in the skeletal muscle. Calves born to MID cows had lower (P = 0.04) glucose concentration than those born to LATE cows. Changes in p-AMPK/AMPK protein, indicated a possible metabolic inflexibility in the skeletal muscle of calves born to CTRL cows. These results indicated that lack of protein supplementation in pregnant cows alter the energy metabolism of their calves and reflect in a metabolic inflexibility.

RESUMO: O objetivo deste estudo foi avaliar o efeito da suplementação proteica materna sobre o metabolismo energético do músculo esquelético de bezerros de corte. Dezesseis matrizes gestantes foram divididas em três grupos: CONTROLE (não suplementado); MÉDIO (suplementados entre 30 e 180 dias de gestação); e FINAL (suplementado entre 181 e 281 dias de gestação). O suplemento continha 30% de proteína bruta e foi fornecido em quantidades totais iguais aos tratamentos. Trinta dias após o nascimento, amostras de sangue e músculo dos bezerros foram coletadas para análises de expressão gênica, abundância de proteínas e metabólitos. Não foram observadas diferenças (P ≥ 0,15) no peso ao nascimento ou parâmetros de desempenho ao desmame, bem como na expressão dos genes avaliados (P ≥ 0,21). Os bezerros nascidos de matrizes do tratamento CONTROLE apresentaram menor proporção (P = 0,03) de proteína p-AMPK/AMPK no músculo esquelético. Os bezerros nascidos de matrizes do tratamento MÉDIO apresentaram concentração de glicose menor (P = 0,04) do que aqueles nascidos de matrizes do tratamento FINAL. Os resultados observados indicam que a ausência de suplementação proteica em matrizes gestantes pode alterar o metabolismo energético da progênie e refletir em uma inflexibilidade metabólica, a qual pode ocasionar limitações quanto à eficiência energética do tecido muscular esquelético e consequentemente, limitar o desempenho da progênie ao longo da fase pós-natal.

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Cattle of Colombian creole breed Chino Santandereano in the raising phase were subjected to stabling for 100 days in order to determine the productive, nutritional, and metabolic capacity when exposed to different degrees of protein-energy supplementation. Sixteen whole males with an initial mean weight of 377.69 ± 16.55 kg were used in completely randomized delineation with four treatments and four repetitions per treatment, as follows: UNS, not supplemented; low, supplemented with amounts relative to 0.5% of body weight; medium, supplemented with amounts relative to 1.0% of the live weight; and high, supplemented with amounts relative to 1.5% of body weight. At the end of each experimental period, the animals were weighed, and samples of feed, feces, blood, and urine were collected to determine the performance, consumption, and digestibility of the nutritional components, and nitrogen balance. In the greater performance, consumption, and digestibility of DM, OM, C-NFCP, EE, and NFC, the concentration of ureic nitrogen in blood and urine (P <0.05) was observed in supplemented animals when contrasted with UNS. Bovine Chinese Santandereano presents high productive, nutritional, and metabolic potential as a response to high levels of protein-energy supplementation.

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The objective of this study was to evaluate the effects of strategic supplementation in the dry period and dry/rainy transition period on the performance and nutritional, metabolic, and reproductive responses of Nellore heifers grazing Urochloa decumbens. Forty-eight Nellore heifers with age and body weight (BW) of eight months and 235 ± 3.3 kg, respectively, were used. The experimental design was a completely randomized design, with four treatments, all with 12 replications. The evaluated strategies were as follows: low supplementation (LOHI; 0.2% of BW/heifer/day) in the first 90 days and high supplementation (0.6% of BW/heifer/day) in the 90 days thereafter; average supplementation (AVER) with 0.4% of BW/heifer/day for 180 days; high supplementation (HILO; 0.6% of BW/heifer/day) in the first 90 days and low supplementation (0.2% of BW/heifer/day) in the 90 days thereafter; only mineral mix (MM) ad libitum during the 180 days. Data were evaluated using orthogonal contrasts. Supplementation improved the performance of the animals during of dry period (P < 0.05) and dry/rainy transition period (P < 0.05). Supplemented animals had higher longissimus muscle area (LMA) and subcutaneous fat thickness (SFT) at the end of the experiment (P < 0.05). Multiple supplementation increased intake of dry matter (DM), organic matter (OM), and crude protein (CP) in kg/day throughout the experiment. The supplementation increased the digestibility of DM, OM, CP, apNDF, and TDN (P < 0.05). Serum urea nitrogen (SUN), glucose (GLUC), insulin (INS), and progesterone (PROG) were higher in supplemented heifers (P < 0.05). Supplementation reduced the concentrations of non-esterified fatty acids (NEFA) (P < 0.05) and increased conception rate (P < 0.05). In summary, the supplementation strategies adopted in this study improve the performance, metabolic status, and carcass traits of heifers under grazing, allowing an improvement in the conception rate of 15-month-old Nellore heifers.

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The objective of this study was to evaluate the effects of supplementation strategy on performance, nutritional and metabolic characteristics of Nellore suckling female calves, on grazing Urochloa decumbens during the rainy-dry transition period. Forty-four Nellore female calves, averaging 147.6 ± 1.34 kg (4 months), were used. A single supplement 20% of crude protein of dry matter was provided, consisting of daily supplement at 0.0, 0.2, 0.4, or 0.6% of body weight. A positive linear effect (P < 0.05) on final body weight and average weight gain of female calves was observed with increased supplementation. Supplementation increased consumption, in kilograms per day, of dry matter (DM), organic matter (OM), crude protein (CP), digested dry matter, and total digestible nutrients (TDN), all calculated as % of dry matter. There was no effect of supplementation on the consumption in kilograms per day of neutral detergent fiber corrected for ash and protein (apNDF) (P > 0.05). Supplementation increased, in a quadratic way, the total apparent digestibility coefficient of DM, OM, CP, apNDF, and TDN (P < 0.05). There was no effect of supplementation (P > 0.05) on non-esterified fatty acid concentrations. There was a positive linear effect (P < 0.05) of supplementation on insulin concentrations. In this study, it is concluded that linearly increasing the feeding level of a supplement with 20% crude protein (% of DM) in the range of 0.2-0.6% of body weight improves the performance, nutritional and metabolic characteristics of the animals, considering these pasture characteristics.

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Beef cows' milk yield is typically determined by measuring milk yield once daily and then doubling this value to estimate daily production. However, it is not known whether this is accurate. Thus, we aimed to determine the association between morning and afternoon milk yield in grazing Nellore cows. Eighty Nellore cows were used, with initial weight of 516.0 ± 1.0 kg. The experiment was a completely randomized factorial scheme, with 20 replications and four treatments (i.e., + or - pre-partum supplementation in combination with + or - post-partum supplementation): PRMM-1 kg of supplement/cow/day for 90 days pre-partum; MMPS-1 kg of supplement/cow/day for 90 days post-partum; PRPS-1 kg of supplement/cow/day for 90 days pre-partum and 90 days post-partum; and MM-only mineral mix ad libitum during pre- and post-partum. Milk was sampled on days 45, 135, and 225 post-partum (early, middle, and late lactation, respectively). No effects were observed of pre- and post-partum supplementation on milk yield (P > 0.05). The afternoon/morning proportion of 0.45 in the early third of lactation was higher than other stages, which had a proportion of 0.41 (P < 0.05). Post-partum supplementation increased milk protein in the morning and afternoon milking (P < 0.05). There was also no effect of pre- and post-partum supplementation on afternoon-morning proportion other milk components (P > 0.05). We conclude that estimating daily milk production of grazing beef cattle by multiplying a once daily milking amount times two is not accurate. Under the conditions of this study, proportion of total daily production represented by the ratio of afternoon/morning milking was 0.45 in early lactation (first third) and 0.41 in mid- and late lactation.

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The aim of this study was to evaluate the effect of different energy sources - corn, sorghum, and wheat bran - on performance, intake, digestibility, and nitrogen utilization efficiency of beef calves subjected to creep feeding. Thirty-four calves (12 males and 22 females) with initial age and weight of four months and 108±3.9 kg, respectively, were used in a randomized-block experimental design with four treatments and two blocks (males and females). Treatments were control (MM), a mineral mixture ad libitum; corn (C); corn + sorghum (C+S); and corn + sorghum + wheat bran (C+S+WB). Supplements contained approximately 20% crude protein (CP), and were fed in the amount of 0.5% of body weight. Supplemented animals had higher performance (P 0.05) when compared with those on treatment MM. Supplementation increased (P 0.05) the intakes of dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fiber corrected for ash and protein (NDFap), non-fibrous carbohydrates (NFC), digestible neutral detergent fiber (dNDF), and digestible organic matter (dOM). The digestibility of NFC was higher (P 0.05) in the supplemented treatments. The C+S and C+S+WB treatments provided increased digestibility (P 0.05) of OM, CP, and NDFap. The synthesis of nitrogen compounds was higher (P 0.05) in animals on treatment C as compared with those on the other supple...(AU)

O objetivo deste trabalho foi avaliar o efeito de diferentes fontes energéticas, milho, sorgo e farelo de trigo, sobre o desempenho, o consumo, a digestibilidade aparente total dos constituintes da dieta e a eficiência de utilização dos compostos nitrogenados em bezerros de corte submetidos ao creep feeding. Foram utilizados 34 bezerros lactentes (12 machos e 22 fêmeas) com idade e peso médio inicial de quatro meses e 108±3,9 kg, respectivamente. O experimento foi conduzido em blocos casualizados com quatro tratamentos e dois blocos (machos e fêmeas). Os tratamentos foram: controle (MM), fornecimento de mistura mineral ad libitum aos animais; milho (M); milho + sorgo (M+S) e milho + sorgo + farelo de trigo (M+S+FT). Os suplementos continham aproximadamente 20% de proteína bruta (PB) e foram fornecidos na quantidade de 5 g kg-1do peso corporal. Os animais suplementados apresentaram maior (P 0,05) ganho de peso quando comparados com os animais do tratamento MM. A suplementação aumentou (P 0,05) os consumos de matéria seca (MS), matéria orgânica (MO), PB, fibra em detergente neutro corrigida para cinzas e proteína (FDNcp), carboidrato não fibroso (CNF), fibra em detergente neutro digerida (FDNd) e matéria orgânica digerida (MOD). A digestibilidade aparente total do CNF foi maior (P 0,05) nos animais suplementados. Os tratamentos M+S e M+S+FT apresentaram maior...(AU)

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RESUMO

The aim of this study was to evaluate the effect of different energy sources - corn, sorghum, and wheat bran - on performance, intake, digestibility, and nitrogen utilization efficiency of beef calves subjected to creep feeding. Thirty-four calves (12 males and 22 females) with initial age and weight of four months and 108±3.9 kg, respectively, were used in a randomized-block experimental design with four treatments and two blocks (males and females). Treatments were control (MM), a mineral mixture ad libitum; corn (C); corn + sorghum (C+S); and corn + sorghum + wheat bran (C+S+WB). Supplements contained approximately 20% crude protein (CP), and were fed in the amount of 0.5% of body weight. Supplemented animals had higher performance (P 0.05) when compared with those on treatment MM. Supplementation increased (P 0.05) the intakes of dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fiber corrected for ash and protein (NDFap), non-fibrous carbohydrates (NFC), digestible neutral detergent fiber (dNDF), and digestible organic matter (dOM). The digestibility of NFC was higher (P 0.05) in the supplemented treatments. The C+S and C+S+WB treatments provided increased digestibility (P 0.05) of OM, CP, and NDFap. The synthesis of nitrogen compounds was higher (P 0.05) in animals on treatment C as compared with those on the other supple...

O objetivo deste trabalho foi avaliar o efeito de diferentes fontes energéticas, milho, sorgo e farelo de trigo, sobre o desempenho, o consumo, a digestibilidade aparente total dos constituintes da dieta e a eficiência de utilização dos compostos nitrogenados em bezerros de corte submetidos ao creep feeding. Foram utilizados 34 bezerros lactentes (12 machos e 22 fêmeas) com idade e peso médio inicial de quatro meses e 108±3,9 kg, respectivamente. O experimento foi conduzido em blocos casualizados com quatro tratamentos e dois blocos (machos e fêmeas). Os tratamentos foram: controle (MM), fornecimento de mistura mineral ad libitum aos animais; milho (M); milho + sorgo (M+S) e milho + sorgo + farelo de trigo (M+S+FT). Os suplementos continham aproximadamente 20% de proteína bruta (PB) e foram fornecidos na quantidade de 5 g kg-1do peso corporal. Os animais suplementados apresentaram maior (P 0,05) ganho de peso quando comparados com os animais do tratamento MM. A suplementação aumentou (P 0,05) os consumos de matéria seca (MS), matéria orgânica (MO), PB, fibra em detergente neutro corrigida para cinzas e proteína (FDNcp), carboidrato não fibroso (CNF), fibra em detergente neutro digerida (FDNd) e matéria orgânica digerida (MOD). A digestibilidade aparente total do CNF foi maior (P 0,05) nos animais suplementados. Os tratamentos M+S e M+S+FT apresentaram maior...

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