RESUMO
The objective of this study was to quantify argyrophillic, argentaffin and insulin immunoreactive endocrine cells in the different segments of the small intestine of Didelphis aurita and measure probable differences in the number of these cells between adult and post-pubertal animals. Biological material consisted of ten male and female opossums specimen, divided in two groups according to weigh. The utilized staining techniques were Grimelius, modified Masson-Fontana and direct immunoperoxidase. Results indicated a predominance of argyrophillic cells in the small intestine of opossums from class 1 and 2, with an average of 52.58 and 56.15 cells mm-2, respectively; of which, the average number of total endocrine cells, argyrophillic and argentaffin cells decreased distally in the intestinal segments of opossums from classes 1 and 2. No significant difference was observed for the insulin immunoreactive cells between the intestinal segments of animals from class 2. A greater number of insulin immunoreactive cells was encountered in the jejunum and ileum of animals from class 2 when compared to the same segment in animals from class 1.
The objective of this study was to quantify argyrophillic, argentaffin and insulin immunoreactive endocrine cells in the different segments of the small intestine of Didelphis aurita and measure probable differences in the number of these cells between adult and post-pubertal animals. Biological material consisted of ten male and female opossums specimen, divided in two groups according to weigh. The utilized staining techniques were Grimelius, modified Masson-Fontana and direct immunoperoxidase. Results indicated a predominance of argyrophillic cells in the small intestine of opossums from class 1 and 2, with an average of 52.58 and 56.15 cells mm-2, respectively; of which, the average number of total endocrine cells, argyrophillic and argentaffin cells decreased distally in the intestinal segments of opossums from classes 1 and 2. No significant difference was observed for the insulin immunoreactive cells between the intestinal segments of animals from class 2. A greater number of insulin immunoreactive cells was encountered in the jejunum and ileum of animals from class 2 when compared to the same segment in animals from class 1.
RESUMO
The objective of this study was to quantify argyrophillic, argentaffin and insulin immunoreactive endocrine cells in the different segments of the small intestine of Didelphis aurita and measure probable differences in the number of these cells between adult and post-pubertal animals. Biological material consisted of ten male and female opossums specimen, divided in two groups according to weigh. The utilized staining techniques were Grimelius, modified Masson-Fontana and direct immunoperoxidase. Results indicated a predominance of argyrophillic cells in the small intestine of opossums from class 1 and 2, with an average of 52.58 and 56.15 cells mm-2, respectively; of which, the average number of total endocrine cells, argyrophillic and argentaffin cells decreased distally in the intestinal segments of opossums from classes 1 and 2. No significant difference was observed for the insulin immunoreactive cells between the intestinal segments of animals from class 2. A greater number of insulin immunoreactive cells was encountered in the jejunum and ileum of animals from class 2 when compared to the same segment in animals from class 1.
The objective of this study was to quantify argyrophillic, argentaffin and insulin immunoreactive endocrine cells in the different segments of the small intestine of Didelphis aurita and measure probable differences in the number of these cells between adult and post-pubertal animals. Biological material consisted of ten male and female opossums specimen, divided in two groups according to weigh. The utilized staining techniques were Grimelius, modified Masson-Fontana and direct immunoperoxidase. Results indicated a predominance of argyrophillic cells in the small intestine of opossums from class 1 and 2, with an average of 52.58 and 56.15 cells mm-2, respectively; of which, the average number of total endocrine cells, argyrophillic and argentaffin cells decreased distally in the intestinal segments of opossums from classes 1 and 2. No significant difference was observed for the insulin immunoreactive cells between the intestinal segments of animals from class 2. A greater number of insulin immunoreactive cells was encountered in the jejunum and ileum of animals from class 2 when compared to the same segment in animals from class 1.
RESUMO
No presente trabalho foram analisadas diferenças histológicas entre o duodeno, jejuno e íleo em relação às camadas mucosa, submucosa e muscular de gambás da espécie Didelphis aurita Wied-Neuwied, 1826. Verificou-se também a existência de possível relação entre a morfologia da parede intestinal e o número de células endócrinas. Foram capturados dez espécimes de gambás da espécie D. auritaacima de 800g. As técnicas de coloração utilizadas visaram a identificação das células argirófilas (Grimelius), argentafins (Masson Fontana modificada), imunorreativas à insulina (imunoperoxidase direta) e das camadas da parede intestinal (HE). Entre as regiões inicial, média e final de cada segmento, a morfometria das camadas foi similar (P>0,05). As diferenças nos números de células enteroendócrinas não foram acompanhadas de alterações nas espessuras das camadas mucosa, submucosa e muscular do intestino delgado de D. aurita (P>0,05), embora fisiologicamente, os resultados aqui encontrados possam ser importantes.
RESUMO
No presente trabalho foram analisadas diferenças histológicas entre o duodeno, jejuno e íleo em relação às camadas mucosa, submucosa e muscular de gambás da espécie Didelphis aurita Wied-Neuwied, 1826. Verificou-se também a existência de possível relação entre a morfologia da parede intestinal e o número de células endócrinas. Foram capturados dez espécimes de gambás da espécie D. auritaacima de 800g. As técnicas de coloração utilizadas visaram a identificação das células argirófilas (Grimelius), argentafins (Masson Fontana modificada), imunorreativas à insulina (imunoperoxidase direta) e das camadas da parede intestinal (HE). Entre as regiões inicial, média e final de cada segmento, a morfometria das camadas foi similar (P>0,05). As diferenças nos números de células enteroendócrinas não foram acompanhadas de alterações nas espessuras das camadas mucosa, submucosa e muscular do intestino delgado de D. aurita (P>0,05), embora fisiologicamente, os resultados aqui encontrados possam ser importantes.