RESUMO
Physiological parameters of laboratory animals used for biomedical research is crucial for following several experimental procedures. With the intent to establish baseline biologic parameters for non-human primates held in closed colonies, hematological and morphometric data of captive monkeys were determined. Data of clinically healthy rhesus macaques (Macaca mulatta), cynomolgus monkeys (Macaca fascicularis), and squirrel monkeys (Saimiri sciureus) were collected over a period of five years. Animals were separated according to sex and divided into five age groups. Hematological data were compared with those in the literature by Student's t test. Discrepancies with significance levels of 0.1, 1 or 5% were found in the hematological studies. Growth curves showed that the sexual dimorphism of rhesus monkeys appeared at an age of four years. In earlier ages, the differences between sexes could not be distinguished (p < 0.05). Sexual dimorphism in both squirrel monkeys and cynomolgus monkeys occurred at an age of about 32 months. Data presented in this paper could be useful for comparative studies using primates under similar conditions.
Assuntos
Macaca fascicularis/anatomia & histologia , Macaca mulatta/anatomia & histologia , Saimiri/anatomia & histologia , Caracteres Sexuais , Fatores Etários , Animais , Biometria , Feminino , Macaca fascicularis/sangue , Macaca fascicularis/crescimento & desenvolvimento , Macaca mulatta/sangue , Macaca mulatta/crescimento & desenvolvimento , Masculino , Saimiri/sangue , Saimiri/crescimento & desenvolvimentoRESUMO
Physiological parameters of laboratory animals used for biomedical research is crucial for following several experimental procedures. With the intent to establish baseline biologic parameters for non-human primates held in closed colonies, hematological and morphometric data of captive monkeys were determined. Data of clinically healthy rhesus macaques (Macaca mulatta), cynomolgus monkeys (Macaca fascicularis), and squirrel monkeys (Saimiri sciureus) were collected over a period of five years. Animals were separated according to sex and divided into five age groups. Hematological data were compared with those in the literature by Student's t test. Discrepancies with significance levels of 0.1, 1 or 5 percent were found in the hematological studies. Growth curves showed that the sexual dimorphism of rhesus monkeys appeared at an age of four years. In earlier ages, the differences between sexes could not be distinguished (p < 0.05). Sexual dimorphism in both squirrel monkeys and cynomolgus monkeys occurred at an age of about 32 months. Data presented in this paper could be useful for comparative studies using primates under similar conditions.
Assuntos
Animais , Masculino , Feminino , Macaca fascicularis , Macaca mulatta , Saimiri , Caracteres Sexuais , BiometriaRESUMO
Trypanosoma evansi (Trypanosomatidae, Kinetoplastida) is a salivarian trypanosomatid that infects eight mammal orders spread over America, Europe and Asia. In Brazil, T. evansi is the etiological agent of "Mal de Cadeiras", a horse disease very often described in the region known as Pantanal do Mato Grosso. Few data concerning the genetic diversity and biology of subpopulations of T. evansi that circulate in Brazil are available. The factors that modulate the interaction of this parasite with its hosts also remain to be elucidated. Here we evaluated the course of experimental infection of six T. evansi isolates derived from domestic and wild animals in Swiss-Webster mice and three Mus musculus lineages. The follow-up included biological, immunological as well as biochemical and hematological parameters. The same isolates as well as three others were characterized by pulsed-field electrophoresis. Our results showed that T. evansi isolates displayed significant differences regarding behavior and morbidity patterns in the distinct mouse lineages. Nevertheless, these differences could not be correlated with pulsed-field electrophoresis profiles. Indeed, concerning this molecular marker, only microheterogeneity was observed. Moreover, we observed that the outcome of the infection is defined by both host genetic background and peculiarities (virulence factors) of the distinct T. evansi isolates. Anemia and hypoglycemia were the only features that could be observed in all mouse lineages, independently of the inoculated T. evansi subpopulation. In addition, our data also show that Mus musculus is a suitable model host for the study of the different pathogenetic features of T. evansi infection.
Assuntos
Trypanosoma/patogenicidade , Tripanossomíase/etiologia , Animais , Anticorpos Antiprotozoários/biossíntese , Brasil , Doenças do Cão/etiologia , Cães , Doenças dos Cavalos/etiologia , Cavalos , Cariotipagem , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C3H , Camundongos Endogâmicos C57BL , Especificidade da Espécie , Trypanosoma/genética , Trypanosoma/imunologia , Trypanosoma/isolamento & purificação , Tripanossomíase/parasitologia , Tripanossomíase/veterináriaRESUMO
Para verificar a incidência de sífilis na populaçäo carcerária do Instituto Penal de Campo Grande, foram realizadas reaçöes de VDRL de 112 detentos. Os resultados positivos foram confirmados através da reaçäo de FTA-ABS. Foi encontrado um resultado de 10,7% de positividade, sendo estes indivíduos encaminhados para tratamento específico. Além do levantamento, foram realizados ciclos de palestras educativas sobre doenças sexualmente transmitidas