RESUMO

Macro domains consist of 130-190 amino acid residues and appear to be highly conserved in all kingdoms of life. Intense research on this field has shown that macro domains bind ADP-ribose and other similar molecules, but their exact function still remains intangible. Macro domains are highly conserved in the Alphavirus genus and the Venezuelan equine encephalitis virus (VEEV) is a member of this genus that causes fatal encephalitis to equines and humans. In this study we report the high yield recombinant expression and preliminary solution NMR study of the macro domain of VEEV. An almost complete sequence-specific assignment of its (1)H, (15)N and (13)C resonances was obtained and its secondary structure predicted by TALOS+. The protein shows a unique mixed α/ß-fold.

Assuntos

Espectroscopia de Ressonância Magnética Nuclear de Carbono-13 , Vírus da Encefalite Equina Venezuelana/metabolismo , Ressonância Magnética Nuclear Biomolecular , Espectroscopia de Prótons por Ressonância Magnética , Proteínas não Estruturais Virais/química , Sequência de Aminoácidos , Dados de Sequência Molecular , Isótopos de Nitrogênio , Estrutura Terciária de Proteína , Alinhamento de Sequência