1.
Braz. j. biol
; Braz. j. biol;34(1): 5-7, 2003.
Artigo
em Inglês
| LILACS, Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP, SESSP-IBACERVO
| ID: biblio-1061606
RESUMO
Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinalattachment-and-effacement lesion (A/E). Through immunoblotting, immunofluorescence, flow citometry andimmunogold we observed that the obtained polyclonal antibody against conserved intimin region recognizesthe different intimin subtypes and suggests that it can be used as a tool for EPEC and EHEC detection.Besides, immuno-dot assay seems to be a possible alternative as a capture method.