RESUMO

The potential of chitosan as a blood lead chelator and an antioxidant had been proven, yet its ability was still not optimal. Chitosan had a relatively large molecular size, which reduced the effectivity of its distribution towards the tissues. Gamma Co-60 irradiation technique was presumably applicable to solve the issue. The antioxidant activity of chitosan could also be optimized by the addition of vitamin E. This study aimed to analyze the antioxidant activity of the combination of Gamma Co-60 irradiated chitosan and vitamin E in lead acetate-induced rats. Twenty-four rats, which were distributed in six groups, were treated using the combination of gamma Co-60 irradiated chitosan at a dose of 150 kGy and vitamin E 1000 IU. All groups, except for the naïve group, were induced with lead acetate. The positive control group was induced with only lead acetate, while treatment group 1 had an additional treatment of irradiated chitosan. The treatment groups 2-4 were treated using the combination of irradiated chitosan and vitamin E in increasing doses respectively for forty days. Blood serum was collected for measurement of Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx), and Malondialdehyde (MDA). The results showed that the providedtreatment increased the enzymatic activity of SOD, CAT, and GPx, and reduced the MDA level in lead acetate-induced rats. However, as the vitamin E dosage was increased, it posed several side effects. It was concluded that the combination Gamma Co-60 irradiated chitosan and vitamin E increased the activity of various endogenous antioxidant enzymes and decreased lipid peroxidation, dependent on the amount of vitamin E.(AU)

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RESUMO

This article discusses the research results on the synergetic effect of chitosan and vitamin C in overcoming free radical effect due to blood lead (Pb2+) accumulation. Blood lead level and enzymatic activities of superoxide dismutase (SOD), catalase oxidase (CAT), and Glutathione peroxidase (GPx) were used as the main parameters. Thirty adult male albino rats were divided into six groups. Group 1 was normal control group; group 2 was the negative control group treated with lead acetate at 175 mg kg-1 body weight (BW). Group 3 was treated with 64 mg kg-1 BW of chitosan day-1. Group 4, 5, and 6 were treated with chitosan and vitamin C combination at the dose of 100, 200, and 300 mg kg-1 BW, respectively. All groups were inducted using 175 mg kg-1 BW of Pb-acetate, excluding control group. Results showed that chitosan and vitamin C treatment at the dose of 300 mg kg-1 BW decreased blood Pb2+ level in rats exposed to Pb-acetate. The combination also significantly increased enzymatic activities from SOD, CAT, and GPx compared to the other groups. In conclusion, the combination of chitosan and vitamin C could elevate the several antioxidative enzymes activities in Pb-acetate induced rats.

Este artigo discute os resultados da pesquisa sobre o efeito sinérgico da quitosana e da vitamina C na superação do efeito dos radicais livres devido ao acúmulo de chumbo no sangue (Pb2+). O nível de chumbo no sangue e as atividades enzimáticas de superóxido dismutase (SOD), catalase oxidase (CAT) e glutationa peroxidase (GPx) foram utilizados como parâmetros principais. Trinta ratos albinos adultos foram divididos em seis grupos. Grupo 1 foi grupo controle normal; o grupo 2 foi o grupo controle negativo tratado com acetato de chumbo a 175 mg kg-1 de peso corporal (PC). O grupo 3 foi tratado com 64 mg kg-1 PC de quitosana dia-1. Os grupos 4, 5 e 6 foram tratados com combinação de quitosana e vitamina C nas doses de 100, 200 e 300 mg kg-1 PC, respectivamente. Todos os grupos foram induzidos usando 175 mg kg-1 de PC de Pb-acetato, excluindo o grupo controle. Os resultados mostraram que o tratamento com quitosana e vitamina C na dose de 300 mg kg-1 PC diminuiu o nível de Pb2+ no sangue em ratos expostos ao acetato de Pb. A combinação também aumentou significativamente as atividades enzimáticas de SOD, CAT e GPx em comparação com os outros grupos. Em conclusão, a combinação de quitosana e vitamina C pode elevar as várias atividades das enzimas antioxidativas em ratos induzidos com acetato de Pb.

Assuntos

RESUMO

This article discusses the research results on the synergetic effect of chitosan and vitamin C in overcoming free radical effect due to blood lead (Pb2+) accumulation. Blood lead level and enzymatic activities of superoxide dismutase (SOD), catalase oxidase (CAT), and Glutathione peroxidase (GPx) were used as the main parameters. Thirty adult male albino rats were divided into six groups. Group 1 was normal control group; group 2 was the negative control group treated with lead acetate at 175 mg kg-1 body weight (BW). Group 3 was treated with 64 mg kg-1 BW of chitosan day-1. Group 4, 5, and 6 were treated with chitosan and vitamin C combination at the dose of 100, 200, and 300 mg kg-1 BW, respectively. All groups were inducted using 175 mg kg-1 BW of Pb-acetate, excluding control group. Results showed that chitosan and vitamin C treatment at the dose of 300 mg kg-1 BW decreased blood Pb2+ level in rats exposed to Pb-acetate. The combination also significantly increased enzymatic activities from SOD, CAT, and GPx compared to the other groups. In conclusion, the combination of chitosan and vitamin C could elevate the several antioxidative enzymes activities in Pb-acetate induced rats.(AU)

Este artigo discute os resultados da pesquisa sobre o efeito sinérgico da quitosana e da vitamina C na superação do efeito dos radicais livres devido ao acúmulo de chumbo no sangue (Pb2+). O nível de chumbo no sangue e as atividades enzimáticas de superóxido dismutase (SOD), catalase oxidase (CAT) e glutationa peroxidase (GPx) foram utilizados como parâmetros principais. Trinta ratos albinos adultos foram divididos em seis grupos. Grupo 1 foi grupo controle normal; o grupo 2 foi o grupo controle negativo tratado com acetato de chumbo a 175 mg kg-1 de peso corporal (PC). O grupo 3 foi tratado com 64 mg kg-1 PC de quitosana dia-1. Os grupos 4, 5 e 6 foram tratados com combinação de quitosana e vitamina C nas doses de 100, 200 e 300 mg kg-1 PC, respectivamente. Todos os grupos foram induzidos usando 175 mg kg-1 de PC de Pb-acetato, excluindo o grupo controle. Os resultados mostraram que o tratamento com quitosana e vitamina C na dose de 300 mg kg-1 PC diminuiu o nível de Pb2+ no sangue em ratos expostos ao acetato de Pb. A combinação também aumentou significativamente as atividades enzimáticas de SOD, CAT e GPx em comparação com os outros grupos. Em conclusão, a combinação de quitosana e vitamina C pode elevar as várias atividades das enzimas antioxidativas em ratos induzidos com acetato de Pb.(AU)

Assuntos