RESUMO
In order to establish whether tissues damaged by Chronic Periapical Pathology (CPP) of endodontic origin produced heat shock protein (HSP) capable of attracting reactive lymphocytes, paraffin sections of samples from the oral cavity of 10 patients with CPP were incubated with commercially available anti-HSP monoclonal antibodies. Antibodies were evidenced employing the alkaline phosphatase immunoenzymatic method (DAKO). HSP was found in the lamina propria infiltrated by lymphocytes, in six of the ten samples. These results suggest that HSP may be one of the lymphocyte recruiting factors in the damaged area and opens new possibilities for further research.
Assuntos
Proteínas de Choque Térmico/biossíntese , Periodontite Periapical/imunologia , Periodontite Periapical/metabolismo , Adolescente , Adulto , Quimiotaxia de Leucócito , Doença Crônica , Feminino , Proteínas de Choque Térmico/fisiologia , Humanos , Técnicas Imunoenzimáticas , Ativação Linfocitária , Linfócitos/fisiologia , Masculino , Pessoa de Meia-IdadeRESUMO
A comparative study of the apical morphology and contour was performed with the scanning electron microscope at 45x, 70x and 300x, while the chemical composition of eight gutta-percha point brands was surveyed with an EDAX 9100 electron microprobe. They were classified according to their apical morphology as conical (25%) and truncate (75%), based on their contour as regular (50%) and irregular (50%) and according to their surface as without defects (50%) and with defects (50%). The analysis of inorganic compounds revealed the presence of varying proportions of Zn, Ba, Si, Mg. Ca, P, Cl and Al, possibly implying that the presence of Ba and Al could interfere with postendodontic repair or at least irritate the periapical area if inadvertently overfilled.
Assuntos
Guta-Percha/química , Microanálise por Sonda Eletrônica , Microscopia Eletrônica de VarreduraRESUMO
A comparative study of the apical morphology and contour was performed with the scanning electron microscope at 45x, 70x and 300x, while the chemical composition of eight gutta-percha point brands was surveyed with an EDAX 9100 electron microprobe. They were classified according to their apical morphology as conical (25
) and truncate (75
), based on their contour as regular (50
) and irregular (50
) and according to their surface as without defects (50
) and with defects (50
). The analysis of inorganic compounds revealed the presence of varying proportions of Zn, Ba, Si, Mg. Ca, P, Cl and Al, possibly implying that the presence of Ba and Al could interfere with postendodontic repair or at least irritate the periapical area if inadvertently overfilled.
RESUMO
A comparative study of the apical morphology and contour was performed with the scanning electron microscope at 45x, 70x and 300x, while the chemical composition of eight gutta-percha point brands was surveyed with an EDAX 9100 electron microprobe. They were classified according to their apical morphology as conical (25
) and truncate (75
) and irregular (50
) and according to their surface as without defects (50
) and with defects (50
). The analysis of inorganic compounds revealed the presence of varying proportions of Zn, Ba, Si, Mg. Ca, P, Cl and Al, possibly implying that the presence of Ba and Al could interfere with postendodontic repair or at least irritate the periapical area if inadvertently overfilled.