RESUMO
OBJECTIVE: The objective of this study is to investigate techniques for approaching fractured instruments in the root canal and to present possible outcomes in relation to dentin thickness, fracture resistance, technique success, and clinical time. MATERIALS AND METHODS: The bibliographic research was carried out in PubMed, Cochrane, Lilacs, Web of Science, Scopus, grey literature, and manual search. Following the inclusion and exclusion criteria, the titles and abstracts of 506 articles were read. Of these, 60 articles were selected and read in full. Fourteen articles were included in this systematic review. RESULTS: The most studied technique was the ultrasonic technique and obtained good general results. The more apically the instrument is located, the more difficult it is to remove, the more dentin is removed, and the lower the fracture resistance of the tooth. When removal is not possible, the bypass technique can be performed (bypassing), and on weakened teeth or with little dental remnants, the GentleWave System technique can be used. CONCLUSION: This systematic review shows the evidence from laboratory studies that the curvature and the root third in which the fracture of instruments occurred is relevant to fracture resistance and success. The ultrasonic technique got the best results. Well-conducted clinical studies should be performed for clinical practice. CLINICAL RELEVANCE: The approach of fractured instruments in the root canal is efficient with the use of ultrasound, bypass technique, and Gentle Wave System.
Assuntos
Cavidade Pulpar , Tratamento do Canal Radicular , Cavidade Pulpar/diagnóstico por imagem , Falha de Equipamento , Humanos , Preparo de Canal Radicular , UltrassonografiaRESUMO
STATEMENT OF PROBLEM: Reasons for failures of bilayer yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) restorations include the core-veneer interface bond strength. The influence of the veneering method on the bond strength of veneered Y-TZP is unclear. PURPOSE: The purpose of this systematic review and meta-analysis was to evaluate the effect of the veneering method on the bond strength of bilayer Y-TZP. MATERIAL AND METHODS: This review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. Searches were performed on PubMed/MEDLINE, Web of Science (Core Collection), and Scopus for in vitro studies evaluating the effect of the veneering technique on the bond strength of bilayer Y-TZP. Statistical analyses were performed with random-effect models (α=.05). RESULTS: From 619 identified studies, 140 were selected for full-text analysis and 16 were included. Manual searching yielded no additional articles. The meta-analyses comprised 15 studies, and 1 study was included only in the descriptive analysis. Meta-analyses compared the hand-layered method (control) with pressed, fused, and cemented veneering methods. The fused and cemented techniques were analyzed by using subgroups assessing the veneering ceramic type (predominantly glass-ceramics and particle-filled glass-ceramics). Similar bond strength results (P=.540) were found for pressed and hand-layered veneered Y-TZP specimens. Fused veneers achieved higher bond strength values (P<.001) than the hand-layered veneers on Y-TZP frameworks, irrespective of the veneering ceramic type (predominantly glass-ceramics: P=.002; particle-filled glass-ceramics: P<.001). Global and subgroup analyses indicated that lower core-veneer bond strength values (P<.001) were found for cemented in relation to hand-layered veneers. CONCLUSIONS: Core-veneer interfacial adhesion was equivalent for pressed and hand-layered veneering techniques. Improved bond strength, regardless of the veneer ceramic material type, was achieved by the fused veneering method, which seems to be a promising choice for the veneering of Y-TZP. In contrast, the cemented method may be unsuitable for veneering Y-TZP structures because of its lower bond strength than the hand-layered veneering technique.
Assuntos
Porcelana Dentária , Facetas Dentárias , Teste de Materiais , Ítrio , ZircônioRESUMO
Sodium deoxycholate (NaDOC) inhibits the intestinal Ca2+ absorption and ursodeoxycholic acid (UDCA) stimulates it. The aim of this study was to determine whether NaDOC and UDCA produce differential effects on the redox state of duodenal mitochondria altering the Krebs cycle and the electron transport chain (ETC) functioning, which could lead to perturbations in the mitochondrial dynamics and biogenesis. Rat intestinal mitochondria were isolated from untreated and treated animals with either NaDOC, UDCA, or both. Krebs cycle enzymes, ETC components, ATP synthase, and mitochondrial dynamics and biogenesis markers were determined. NaDOC decreased isocitrate dehydrogenase (ICDH) and malate dehydrogenase activities affecting the ETC and ATP synthesis. NaDOC also induced oxidative stress and increased the superoxide dismutase activity and impaired the mitochondrial biogenesis and functionality. UDCA increased the activities of ICDH and complex II of ETC. The combination of both bile acids conserved the functional activities of Krebs cycle enzymes, ETC components, oxidative phosphorylation, and mitochondrial biogenesis. In conclusion, the inhibitory effect of NaDOC on intestinal Ca2+ absorption is mediated by mitochondrial dysfunction, which is avoided by UDCA. The stimulatory effect of UDCA alone is associated with amelioration of mitochondrial functioning. This knowledge could improve treatment of diseases that affect the intestinal Ca2+ absorption.
Assuntos
Colagogos e Coleréticos/farmacologia , Ácido Desoxicólico/farmacologia , Duodeno/efeitos dos fármacos , Mitocôndrias/metabolismo , Ácido Ursodesoxicólico/farmacologia , Animais , Cálcio/farmacocinética , Colagogos e Coleréticos/farmacocinética , Ciclo do Ácido Cítrico/efeitos dos fármacos , Ácido Desoxicólico/farmacocinética , Transporte de Elétrons , Absorção Intestinal/efeitos dos fármacos , Masculino , ATPases Mitocondriais Próton-Translocadoras/metabolismo , Biogênese de Organelas , Fosforilação Oxidativa/efeitos dos fármacos , Estresse Oxidativo , Ratos , Ratos Wistar , Superóxido Dismutase/metabolismo , Ácido Ursodesoxicólico/farmacocinéticaRESUMO
Aim: To evaluate the retention of Y-TZP crowns cemented in aged composite cores ground with burs of different grit sizes. Methods: Sixty composite resin simplified full-crown preparations were scanned, while 60 Y-TZP crowns with occlusal retentions were milled. The composite preparations were stored for 120 days (wet environment-37°C) and randomly distributed into three groups (n=20) according to the type of composite core surface treatment. The groups were defined as: CTRL (control: No treatment), EFB (extra-fine diamond bur [25µm]), and CB (coarse diamond bur [107µm]). The grinding was performed with an adapted surveyor standardizing the speed and pressure of the grinding. The intaglio surfaces on the crowns were air-abraded with silica-coated alumina particles (30 µm) and then a silane was applied. The crowns were cemented with self-adhesive resin cement, thermocycled (12,000 cycles; 5/55°C), stored (120 days) and submitted to a retention test (0.5mm/min). The retentive strength data (MPa) were analyzed using one-way analysis of variance and Tukey test, as well as Weibull analysis. Failures were classified as 50C (above 50% of cement in the crown), 50S (above 50% of cement in the substrate) and COE (composite core cohesive failure). Results: No statistical difference was observed among the retention values (p=0.975). However, a higher Weibull modulus was observed in the CTRL group. The predominant type of failure was 50S (above 50% of cement in the substrate composite). Conclusion: The retention of zirconia crowns was not affected by grinding using diamond burs with different grit sizes (coarse/extra-fine) or when no grinding was performed
Assuntos
Propriedades de Superfície , Zircônio , Colagem Dentária , Retenção em Prótese Dentária , Resinas CompostasRESUMO
Aim: This study evaluated the effect of surface treatments of yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) ceramics on their bond strength to a resin cement. Methods: Seventy zirconia blocks (6 × 6 × 2 mm3, IPS e.max ZirCAD) were assigned into 7 groups (n=10) as-sintered (AS), no treatment; tribochemical silica coating + silanization (TBS; Cojet-sand; ProSil); airabrasion with 45 µm alumina particles + universal primer (AAP; Monobond®Plus); fusion sputtering (FS); SiO2 nanofilm + silanization (SN; ProSil); FS+SN+ silanization (FSSN; ProSil); FS+SN+Universal Primer (FSSNP; Monobond®Plus). Afterwards, a resin cement (RelyX™ ARC) was applied inside cylinders (Ø = 0.96 mm × 1 mm height) placed on the zirconia surfaces. Microshear bond strength tests (µSBS) were carried out (1 mm/min). Failure and phase transformation analysis were performed. Bond strength data (MPa) were subjected to Kruskal-Wallis/Mann Whitney tests. Results: TBS (27 ± 1.2) and AAP (24.7 ± 0.8) showed higher bond strengths than the other groups, followed by FSSNP (15.5 ± 4.2) and FSSN (13.3 ± 3.6). FS (3.4 ± 0.44) and SN (9.5 ± 2.7) showed the lowest values (p < 0.001). Most of the specimens exhibited an adhesive failure. Conclusion: Air-abrasion by silica-coated alumina particles followed by silanization or by alumina particles followed by universal primer resulted in the highest resin bond strength to zirconia. Fusion sputtering and silica nanofilm deposition induced low strengths. However, when these methods are applied in combination and with a primer (FSSN and FSSNP), higher bond strengths may be achieved. Low bond strengths are obtained when no zirconia treatment is performed
Assuntos
Ítrio , Cimentos de Resina , Cimentos Dentários , Resistência à FlexãoRESUMO
BACKGROUND: Bile acids (BAs) are among the main components of bile. Lately, they are also considered important signaling molecules, not only by regulating their own synthesis, but also having a role in several metabolic diseases. OBJECTIVE: In this review we focus on the effect of sodium deoxycholate (NaDOC), ursodeoxycholic (UDCA) and litocholic (LCA) acids and their combination upon the intestinal Ca2+ absorption. To make clear the actions of those BAs on this physiological process, an overview of current information about the mechanisms by which the intestinal Ca2+ occurs is described. METHODS: The PubMed database was searched until 2017, using the keywords bile acids, NaDOC, UDCA and LCA and redox state, apoptosis, autophagy and intestinal Ca2+ absorption. RESULTS: The modulation of redox state, apoptosis and autophagy are mechanisms that are involved in the action of BAs on intestinal Ca2+ absorption. Although the mechanisms are still not completely understood, we provide the latest knowledge regarding the effect of BAs on intestinal Ca2+ absorption. CONCLUSION: The response of the intestine to absorb Ca2+ is affected by BAs, but it is different according to the type and dose of BA. When there is a single administration, NaDOC has an inhibitory effect, UDCA is an stimulator whereas LCA does not have any influence. However, the combination of BAs modifies the response. Either UDCA or LCA protects the intestine against the oxidative injury caused by NaDOC by blocking the oxidative/nitrosative stress, apoptosis and autophagy.
Assuntos
Ácidos e Sais Biliares/metabolismo , Cálcio/metabolismo , Absorção Intestinal/efeitos dos fármacos , Mucosa Intestinal/metabolismo , Animais , Apoptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Ácido Desoxicólico/metabolismo , Humanos , Ácido Litocólico/metabolismo , Ácido Ursodesoxicólico/metabolismoRESUMO
Objective: This study aimed to evaluate the fracture load and displacement of roots restored with posts of different elastic modulus. Material and method: Thirty-six replicas of epoxy resin mixed with glass microfibers were made from an endodontically-treated human premolar root prepared to a length of 12 mm with a custom drill, leaving the apical 4 mm unprepared. Replicas were randomly restored with (n = 12): FP-LM (fiber post with low elastic modulus- 50 GPa), FP-HM (fiber post with high elastic modulus - 67 GPa) and MP (metallic post - 208 GPa), using self-curing adhesive and dual resin cement. Cores were built up with composite resin and metallic crowns were cemented in all the roots with self-adhesive resin cement with self-curing mode. Specimens were subjected to a fracture load test (45° inclination/0.5 mm/min) and displacement was registered at 100 N. Result: One-way ANOVA showed that elastic modulus of the post did not affect the fracture load means (p = 0.203) (FP-LM: 237.4 ± 65.11 N; FP-HM: 236.7 ± 92.85 N; MP: 295.8 ± 108.7 N) but was statistically significant for the displacement (p < 0.00): Tukey's test showed that FP-LM displacement mean (0.81 ± 0.15 mm) was significantly higher than those for FP-HM (0.46 ± 0.26 mm; p = 0.00) and MP (0.62 ± 0.07 mm; p = 0.04). Conclusion: Posts with different elastic modulus exhibit similar fracture loads, but a lower displacement is achieved when fiber posts with a high elastic modulus and metallic posts are used.
Objetivo: Este trabalho avaliou a carga para fratura e deslocamento de raízes restauradas com pinos de diferentes módulos de elasticidade. Material e método: Trinta e seis réplicas de microfibras de vidro embutidas em resina epóxi foram fabricadas a partir de uma raiz de um pré-molar tratado endodonticamente preparado em 12 mm de comprimento com brocas customizadas, deixando 4 mm apicais sem preparo. As raízes foram randomicamente restauradas com (n = 12): FP-LM (pino de fibra com um baixo módulo de elasticidade - 50 GPa), FP-HM (pino de fibra com um alto módulo de elasticidade - 67 GPa) e MP (pinos metálicos - 208 GPa), usando adesivo autopolimerizável e cimento resinoso dual. Núcleos foram confeccionados com resina composta e coroas metálicas foram cimentadas em todas as raízes com cimento resinoso autoadesivo com modo de polimerização químico. Os espécimes foram submetidos ao teste de fratura em 45° (inclinação de 45° / 0,5 mm/min) e o deslocamento foi registrado aos 100 N. Resultado: Anova 1 fator mostrou que o módulo de elasticidade dos retentores não afetou as médias de fratura (p = 0,203) (FP-LM: 237,4 ± 65,11 N; FP-HM: 236,7 ± 92,85 N; MP: 295,8 ± 108,7 N) mas foi estatisticamente significante para o deslocamento (p < 0,00): o teste de Tukey mostrou que a média de deslocamento do grupo FP-LM (0,81 ± 0,15 mm) foi significativamente maior do que o grupo FP-HM (0,46 ± 0,26 mm; p = 0,00) e MP (0,62 ± 0,07 mm; p = 0,47). Conclusão: Pinos com diferentes módulos de elasticidade mostram resistência similar, porém um menor deslocamento é obtido quando pinos de fibra com alto módulo de elasticidade (FP-HM/MP) são usados.
Assuntos
Raiz Dentária , Dente Pré-Molar , Técnica para Retentor Intrarradicular , Prótese Dentária , Resinas Compostas , Cimentos de Resina , Força Compressiva , Coroas , Pinos Dentários , Análise de Variância , Materiais Dentários , Resinas EpóxiRESUMO
The aim of this systematic review was to compare the clinical performance and failure modes of teeth restored with intra-radicular retainers. A search was performed on PubMed/Medline, Central and ClinicalTrials databases for randomized clinical trials comparing clinical behavior and failures of at least two types of retainers. From 341 detected papers, 16 were selected for full-text analysis, of which 9 met the eligibility criteria. A manual search added 2 more studies, totalizing 11 studies that were included in this review. Evaluated retainers were fiber (prefabricated and customized) and metal (prefabricated and cast) posts, and follow-up ranged from 6 months to 10 years. Most studies showed good clinical behavior for evaluated intra-radicular retainers. Reported survival rates varied from 71 to 100% for fiber posts and 50 to 97.1% for metal posts. Studies found no difference in the survival among different metal posts and most studies found no difference between fiber and metal posts. Two studies also showed that remaining dentine height, number of walls and ferrule increased the longevity of the restored teeth. Failures of fiber posts were mainly due to post loss of retention, while metal post failures were mostly related to root fracture, post fracture and crown and/or post loss of retention. In conclusion, metal and fiber posts present similar clinical behavior at short to medium term follow-up. Remaining dental structure and ferrule increase the survival of restored pulpless teeth. Studies with longer follow-up are needed.
Assuntos
Falha de Restauração Dentária , Restauração Dentária Permanente/métodos , Técnica para Retentor Intrarradicular , Dente não Vital/terapia , Viés , Vidro/química , Ouro/química , Humanos , Ensaios Clínicos Controlados Aleatórios como Assunto , Titânio , Resultado do TratamentoRESUMO
LCA and 1,25(OH)2D3 are vitamin D receptor ligands with different binding affinity. The secosteroid stimulates intestinal Ca2+ absorption. Whether LCA alters this process remains unknown. The aim of our work was to determine the effect of LCA on intestinal Ca2+ absorption in the absence or presence of NaDOC, bile acid that inhibits the cation transport. The data show that LCA by itself did not alter intestinal Ca2+ absorption, but prevented the inhibitory effect of NaDOC. The concomitant administration of LCA avoided the reduction of intestinal alkaline phosphatase activity caused by NaDOC. In addition, LCA blocked a decrease caused by NaDOC on gene and protein expression of molecules involved in the transcellular pathway of intestinal Ca2+ absorption. The oxidative stress and apoptosis triggered by NaDOC were abrogated by LCA co-treatment. In conclusion, LCA placed in the intestinal lumen protects intestinal Ca2+ absorption against the inhibitory effects caused by NaDOC. LCA avoids the reduction of the transcellular Ca2+ movement, apparently by blocking the oxidative stress and apoptosis triggered by NaDOC, normalizing the gene and protein expression of molecules involved in Ca2+ movement. Therefore, LCA might become a possible treatment to improve intestinal calcium absorption under oxidant conditions.
Assuntos
Cálcio/metabolismo , Ácido Desoxicólico/antagonistas & inibidores , Duodeno/efeitos dos fármacos , Enterócitos/efeitos dos fármacos , Absorção Intestinal/efeitos dos fármacos , Ácido Litocólico/farmacologia , Fosfatase Alcalina/genética , Fosfatase Alcalina/metabolismo , Animais , Animais Recém-Nascidos , Apoptose/efeitos dos fármacos , Calcitriol/metabolismo , Galinhas , Ácido Desoxicólico/farmacologia , Duodeno/metabolismo , Enterócitos/citologia , Enterócitos/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Absorção Intestinal/fisiologia , Transporte de Íons/efeitos dos fármacos , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Estresse Oxidativo , ATPases Transportadoras de Cálcio da Membrana Plasmática/genética , ATPases Transportadoras de Cálcio da Membrana Plasmática/metabolismo , Carbonilação Proteica/efeitos dos fármacos , Receptores de Calcitriol/genética , Receptores de Calcitriol/metabolismo , Trocador de Sódio e Cálcio/genética , Trocador de Sódio e Cálcio/metabolismoRESUMO
Abstract The aim of this systematic review was to compare the clinical performance and failure modes of teeth restored with intra-radicular retainers. A search was performed on PubMed/Medline, Central and ClinicalTrials databases for randomized clinical trials comparing clinical behavior and failures of at least two types of retainers. From 341 detected papers, 16 were selected for full-text analysis, of which 9 met the eligibility criteria. A manual search added 2 more studies, totalizing 11 studies that were included in this review. Evaluated retainers were fiber (prefabricated and customized) and metal (prefabricated and cast) posts, and follow-up ranged from 6 months to 10 years. Most studies showed good clinical behavior for evaluated intra-radicular retainers. Reported survival rates varied from 71 to 100% for fiber posts and 50 to 97.1% for metal posts. Studies found no difference in the survival among different metal posts and most studies found no difference between fiber and metal posts. Two studies also showed that remaining dentine height, number of walls and ferrule increased the longevity of the restored teeth. Failures of fiber posts were mainly due to post loss of retention, while metal post failures were mostly related to root fracture, post fracture and crown and/or post loss of retention. In conclusion, metal and fiber posts present similar clinical behavior at short to medium term follow-up. Remaining dental structure and ferrule increase the survival of restored pulpless teeth. Studies with longer follow-up are needed.
Assuntos
Humanos , Técnica para Retentor Intrarradicular , Dente não Vital/terapia , Falha de Restauração Dentária , Restauração Dentária Permanente/métodos , Titânio , Viés , Ensaios Clínicos Controlados Aleatórios como Assunto , Resultado do Tratamento , Vidro/química , Ouro/químicaRESUMO
We have previously demonstrated that melatonin (MEL) blocks the inhibition of the intestinal Ca(2+) absorption caused by menadione (MEN). The purpose of this study were to determine whether MEL not only restores but also prevents the intestinal Ca(2+) absorption inhibited either by MEN or BSO, two drugs that deplete glutathione (GSH) in different ways, and to analyze the mechanisms by which MEN and MEL alter the movement of Ca(2+) across the duodenum. To know this, chicks were divided into four groups: 1) controls, 2) MEN treated, 3) MEL treated, and 4) treated sequentially with MEN and MEL or with MEN and MEL at the same time. In a set of experiments, chicks treated with BSO or sequentially with BSO and MEL or with BSO and MEL at the same time were used. MEL not only restored but also prevented the inhibition of the chick intestinal Ca(2+) absorption produced by either MEN or BSO. MEN altered the protein expression of molecules involved in the transcellular as well as in the paracellular pathway of the intestinal Ca(2+) absorption. MEL restored partially both pathways through normalization of the O2(-) levels. The nitrergic system was not altered by any treatment. In conclusion, MEL prevents or restores the inhibition of the intestinal Ca(2+) absorption caused by different GSH depleting drugs. It might become one drug for the treatment of intestinal Ca(2+) absorption under oxidant conditions having the advantage of low or null side effects.
Assuntos
Cálcio/metabolismo , Glutationa/metabolismo , Absorção Intestinal/efeitos dos fármacos , Melatonina/farmacologia , Animais , Galinhas , Óxido Nítrico/metabolismo , Superóxidos/metabolismo , Vitamina K 3/farmacologiaRESUMO
The aim of this work was to study the effect of sodium deoxycholate (NaDOC) and ursodeoxycholic acid (UDCA) on Ca(2+) uptake by enterocytes and the underlying mechanisms. Rats were divided into four groups: a) controls, b) treated with NaDOC, c) treated with UDCA d) treated with NaDOC and UDCA. Ca(2+) uptake was studied in enterocytes with different degrees of maturation. Apoptosis, autophagy and NO content and iNOS protein expression were evaluated. NaDOC decreased and UDCA increased Ca(2+) uptake only in mature enterocytes. The enhancement of protein expression of Fas, FasL, caspase-8 and caspase-3 activity by NaDOC indicates triggering of the apoptotic extrinsic pathway, which was blocked by UDCA. NO content and iNOS protein expression were enhanced by NaDOC, and avoided by UDCA. The increment of acidic vesicular organelles and LC3 II produced by NaDOC was also prevented by UDCA. In conclusion, the inhibitory effects of NaDOC on intestinal Ca(2+) absorption occur by decreasing the Ca(2+) uptake by mature enterocytes. NaDOC triggers apoptosis and autophagy, in part as a result of nitrosative stress. In contrast, UDCA increases the Ca(2+) uptake by mature enterocytes, and in combination with NaDOC acts as an antiapoptotic and antiautophagic agent normalizing the transcellular Ca(2+) pathway.
Assuntos
Cálcio/metabolismo , Ácido Desoxicólico/administração & dosagem , Enterócitos/citologia , Enterócitos/fisiologia , Absorção Intestinal/fisiologia , Ácido Ursodesoxicólico/administração & dosagem , Animais , Apoptose/efeitos dos fármacos , Apoptose/fisiologia , Autofagia/efeitos dos fármacos , Autofagia/fisiologia , Células Cultivadas , Relação Dose-Resposta a Droga , Enterócitos/efeitos dos fármacos , Masculino , Ratos , Ratos WistarRESUMO
The intestine is the only gate for the entry of Ca to the body in humans and mammals. The entrance of Ca occurs via paracellular and intracellular pathways. All steps of the latter pathway are regulated by calcitriol and by other hormones. Dietary and pharmacological compounds also modulate the intestinal Ca absorption process. Among them, dietary Ca and P are known to alter the lipid and protein composition of the brush-border and basolateral membranes and, consequently, Ca transport. Ca intakes are below the requirements recommended by health professionals in most countries, triggering important health problems. Chronic low Ca intake has been related to illness conditions such as osteoporosis, hypertension, renal lithiasis and incidences of human cancer. Carbohydrates, mainly lactose, and prebiotics have been described as positive modulators of intestinal Ca absorption. Apparently, high meat proteins increase intestinal Ca absorption while the effect of dietary lipids remains unclear. Pharmacological compounds such as menadione, dl-butionine-S,R-sulfoximine and ursodeoxycholic acid also modify intestinal Ca absorption as a consequence of altering the redox state of the epithelial cells. The paracellular pathway of intestinal Ca absorption is poorly known and is under present study in some laboratories. Another field that needs to be explored more intensively is the influence of the gene × diet interaction on intestinal Ca absorption. Health professionals should be aware of this knowledge in order to develop nutritional or medical strategies to stimulate the efficiency of intestinal Ca absorption and to prevent diseases.
RESUMO
OBJECTIVE: Considering that periodontal ligament simulation may influence the stress distribution over teeth restored with intraradicular retainers, this study aimed to assess the combined effect of mechanical cycling and periodontal ligament simulation on both the bond strength between fiber posts and root dentin and the fracture resistance of teeth restored using glass fiber posts. MATERIAL AND METHODS: Ninety roots were randomly distributed into 3 groups (n=10) (C-MC: control; P-MC: polyether; AS-MC: addition silicone) to test bond strength and 6 groups (n=10) (C: control; P: polyether; AS: addition silicone, without mechanical cycling, and C-MC, P-MC and AS-MC with mechanical cycling) to test fracture strength, according to the material used to simulate the periodontal ligament. For the bond strength test, fiber posts were cemented, cores were built, mechanical cycling was applied (2×10(6) cycles, 88 N, 2.2 Hz, and 45º incline), and the teeth cut into 3 slices (2 mm), which were then subjected to the push-out test at 1 mm/min. For the fracture strength test, fiber posts were cemented, cores were built, and half of the groups received mechanical cycling, followed by the compressive strength (45° to the long axis and 1 mm/min) performed on all groups. RESULTS: Periodontal ligament simulation did not affect the bond strength (p=0.244) between post and dentin. Simulation of periodontal ligament (p=0.153) and application of mechanical cycling (p=0.97) did not affect fracture resistance. CONCLUSIONS: The materials used to simulate the periodontal ligament did not affect fracture or bond strength, therefore periodontal ligament simulation using the tested materials could be considered optional in the conditions of the study.
Assuntos
Colagem Dentária/métodos , Ligamento Periodontal , Técnica para Retentor Intrarradicular , Fraturas dos Dentes , Animais , Bovinos , Resinas Compostas/química , Força Compressiva , Falha de Restauração Dentária , Análise do Estresse Dentário , Vidro/química , Teste de Materiais , Microscopia Eletrônica de Varredura , Distribuição Aleatória , Reprodutibilidade dos Testes , Estresse Mecânico , Propriedades de Superfície , Resistência à TraçãoRESUMO
Objective: Considering that periodontal ligament simulation may influence the stress distribution over teeth restored with intraradicular retainers, this study aimed to assess the combined effect of mechanical cycling and periodontal ligament simulation on both the bond strength between fiber posts and root dentin and the fracture resistance of teeth restored using glass fiber posts. Material and Methods: Ninety roots were randomly distributed into 3 groups (n=10) (C-MC: control; P-MC: polyether; AS-MC: addition silicone) to test bond strength and 6 groups (n=10) (C: control; P: polyether; AS: addition silicone, without mechanical cycling, and C-MC, P-MC and AS-MC with mechanical cycling) to test fracture strength, according to the material used to simulate the periodontal ligament. For the bond strength test, fiber posts were cemented, cores were built, mechanical cycling was applied (2×106 cycles, 88 N, 2.2 Hz, and 45º incline), and the teeth cut into 3 slices (2 mm), which were then subjected to the push-out test at 1 mm/min. For the fracture strength test, fiber posts were cemented, cores were built, and half of the groups received mechanical cycling, followed by the compressive strength (45° to the long axis and 1 mm/min) performed on all groups. Results: Periodontal ligament simulation did not affect the bond strength (p=0.244) between post and dentin. Simulation of periodontal ligament (p=0.153) and application of mechanical cycling (p=0.97) did not affect fracture resistance. Conclusions: The materials used to simulate the periodontal ligament did not affect fracture or bond strength, therefore periodontal ligament simulation using the tested materials could be considered optional in the conditions of the study. .
Assuntos
Animais , Bovinos , Colagem Dentária/métodos , Ligamento Periodontal , Técnica para Retentor Intrarradicular , Fraturas dos Dentes , Resinas Compostas/química , Força Compressiva , Falha de Restauração Dentária , Análise do Estresse Dentário , Vidro/química , Teste de Materiais , Microscopia Eletrônica de Varredura , Distribuição Aleatória , Reprodutibilidade dos Testes , Estresse Mecânico , Propriedades de Superfície , Resistência à TraçãoRESUMO
The aim of this study was to investigate the effect of ursodeoxycholic acid (UDCA) on intestinal Ca(2+) absorption and to find out whether the inhibition of this process caused by NaDOC could be prevented by UDCA. Chicks were employed and divided into four groups: (a) controls, (b) treated with 10mM NaDOC, (c) treated with 60 µg UDCA/100g of b.w., and (d) treated with 10mM NaDOC and 60 µg UDCA/100g of b.w. UDCA enhanced intestinal Ca(2+) absorption, which was time and dose-dependent. UDCA avoided the inhibition of intestinal Ca(2+) absorption caused by NaDOC. Both bile acids altered protein and gene expression of molecules involved in the transcellular pathway of intestinal Ca(2+) absorption, but in the opposite way. UDCA aborted the oxidative stress produced by NaDOC in the intestine. UDCA and UDCA plus NaDOC increased vitamin D receptor protein expression. In conclusion, UDCA is a beneficial bile acid for intestinal Ca(2+) absorption. Contrarily, NaDOC inhibits the intestinal cation absorption through triggering oxidative stress. The use of UDCA in patients with cholestasis would be benefited because of the protective effect on the intestinal Ca(2+) absorption, avoiding the inhibition caused by hydrophobic bile acids and neutralizing the oxidative stress.
Assuntos
Cálcio/metabolismo , Ácido Desoxicólico/farmacologia , Absorção Intestinal/efeitos dos fármacos , Ácido Ursodesoxicólico/farmacologia , Fosfatase Alcalina/metabolismo , Animais , Galinhas , Relação Dose-Resposta a Droga , Interações Medicamentosas , Regulação da Expressão Gênica/efeitos dos fármacos , Masculino , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Ratos , Fatores de TempoRESUMO
Introduction: The occurrence of canine impaction is not a rare phenomenon, but transmigration of the tooth across the midline is a less common event. The finding is normally asymptomatic and the etiologic factors involved in the transmigration process are still unclear. Objective: To present a clinical case of surgical treatment of a transmigrated mandibular canine. Case report: A 17-year-old male patient presented to the dental clinic to remove an unerupted canine. Clinical and radiological examinations led to the diagnosis of a transmigrated canine in the mental region. Surgical removal was the treatment of choice and further radiographs were needed to complement the clinical exam and to determine the location of the tooth precisely for the surgical procedure. Postoperative period was uneventful. Canine transmigration is a rare finding and symptoms are usually absent. Conclusion: An early detection is important to plan the treatment and mainly to avoid future complications.
RESUMO
Quercetin (QT) could be considered as a potential therapeutic agent for different diseases due to its antioxidant, anti-inflammatory, antiviral and anticancer properties. This study was designed to investigate the ability of QT to protect the chick intestine against menadione (MEN) induced injury in vivo and in vitro. Four-week old chicks (Gallus gallus) were treated i.p. with 2.5µmol of MEN/kg b.w. or with i.l. 50µM QT or both. QT protected the intestinal Ca(2+) absorption against the inhibition caused by MEN, but QT alone did not modify. Glutathione (GSH) depletion provoked by MEN in chick enterocytes was abolished by QT treatment, whereas QT alone did not modify the intestinal GSH content. The enhancement of GSH peroxidase activity produced by MEN was blocked by QT treatment. In contrast, superoxide dismutase activity remained high after simultaneous treatment of enterocytes with MEN and QT. The flavonol also avoided changes in the mitochondrial membrane permeability (swelling) produced by MEN. The FasL/Fas/caspase-3 pathway was activated by MEN, effect that was abrogated by QT. In conclusion, QT may be useful in preventing inhibition of chick intestinal Ca(2+) absorption caused by MEN or other substances that deplete GSH, by blocking the oxidative stress and the FasL/Fas/caspase-3 pathway activation.
Assuntos
Cálcio/metabolismo , Absorção Intestinal , Quercetina/farmacologia , Vitamina K 3/efeitos adversos , Animais , Antioxidantes/metabolismo , Apoptose , Peso Corporal , Cálcio/sangue , Caspase 3/metabolismo , Permeabilidade da Membrana Celular , Galinhas/metabolismo , Enterócitos/efeitos dos fármacos , Enterócitos/enzimologia , Enterócitos/metabolismo , Ativação Enzimática , Ensaios Enzimáticos , Proteína Ligante Fas/metabolismo , Glutationa/metabolismo , Glutationa Peroxidase/metabolismo , Mucosa Intestinal/metabolismo , Intestinos/efeitos dos fármacos , Intestinos/enzimologia , Mitocôndrias/metabolismo , Estresse Oxidativo , Transdução de SinaisRESUMO
Calcitriol or 1,25(OH)(2)D(3) is a negative growth regulator of breast cancer cells. The aim of this study was to determine whether L-buthionine-S,R-sulfoximine, a glutathione-depleting drug, modifies the antiproliferative effects of 1,25(OH)(2)D(3) on MCF-7 cells. For comparison, we included studies in MCF-7 cells selected for vitamin D resistance and in human mammary epithelial cells transformed with SV40 and ras. Our data indicate that L-buthionine-S,R-sulfoximine enhances the growth inhibition of 1,25(OH)(2)D(3) in all transformed breast cell lines. This effect is mediated by ROS leading to apoptosis. In conclusion, BSO alters redox state and sensitizes breast cancer cells to 1,25(OH)(2)D(3)-mediated apoptosis.
Assuntos
Apoptose/efeitos dos fármacos , Neoplasias da Mama/metabolismo , Butionina Sulfoximina/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Vitamina D/análogos & derivados , Caspases/metabolismo , Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Ativação Enzimática/efeitos dos fármacos , Feminino , Humanos , Oxirredução/efeitos dos fármacos , Vitamina D/farmacologiaRESUMO
High concentrations of sodium deoxycholate (NaDOC) produce toxic effects. This study explores the effect of a single high concentration of NaDOC on the intestinal Ca(2+) absorption and the underlying mechanisms. Chicks were divided into two groups: 1) controls and 2) treated with different concentrations of NaDOC in the duodenal loop for variable times. Intestinal Ca(2+) absorption was measured as well as the gene and protein expressions of molecules involved in the Ca(2+) transcellular pathway. NaDOC inhibited the intestinal Ca(2+) absorption, which was concentration dependent. Ca(2+)-ATPase mRNA decreased by the bile salt and the same occurred with the protein expression of Ca(2+)-ATPase, calbindin D(28k) and Na(+)/Ca(2+) exchanger. NaDOC produced oxidative stress as judged by ROS generation, mitochondrial swelling and glutathione depletion. Furthermore, the antioxidant quercetin blocked the inhibitory effect of NaDOC on the intestinal Ca(2+) absorption. Apoptosis was also triggered by the bile salt, as indicated by the TUNEL staining and the cytochrome c release from the mitochondria. As a compensatory mechanism, enzyme activities of the antioxidant system were all increased. In conclusion, a single high concentration of NaDOC inhibits intestinal Ca(2+) absorption through downregulation of proteins involved in the transcellular pathway, as a consequence of oxidative stress and mitochondria mediated apoptosis.