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1.
Front Cell Dev Biol ; 11: 1116805, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36866275

RESUMO

Disruption of the epigenetic program of gene expression is a hallmark of cancer that initiates and propagates tumorigenesis. Altered DNA methylation, histone modifications and ncRNAs expression are a feature of cancer cells. The dynamic epigenetic changes during oncogenic transformation are related to tumor heterogeneity, unlimited self-renewal and multi-lineage differentiation. This stem cell-like state or the aberrant reprogramming of cancer stem cells is the major challenge in treatment and drug resistance. Given the reversible nature of epigenetic modifications, the ability to restore the cancer epigenome through the inhibition of the epigenetic modifiers is a promising therapy for cancer treatment, either as a monotherapy or in combination with other anticancer therapies, including immunotherapies. Herein, we highlighted the main epigenetic alterations, their potential as a biomarker for early diagnosis and the epigenetic therapies approved for cancer treatment.

2.
Toxicon ; 194: 37-43, 2021 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-33610630

RESUMO

Chemical compounds from skin secretions from toads of Bufonidae family have been long-studied. In the search for new molecules with pharmacological action, the 3ß-OH groups of bufadienolides are commonly derivatised using acetyl groups. This work described the isolation and/or structural elucidation of isolated and derivatised compounds from the venom of the Brazilian anuran Rhinella marina, and their evaluation in in vitro assays. In the methanolic extract of the R. marina venom, compound cholesterol (1) was isolated from the CRV-52 fraction by classic column chromatography, dehydrobufotenine (2) by Sephadex LH-20 from the CRV-28 fraction, and a mix of suberoyl arginine (3) and compound 2 was obtained from the CRV-6-33 fraction. The compounds marinobufagin (4), telocionbufagin (5) and bufalin (6) were isolated by classic column chromatography, followed by separation via HPLC in the CRV-70 fraction, and the compound marinobufotoxin (9) was isolated by classic column chromatography in the CRV-6 fraction, here being isolated for the first time in R. marina specimens. Compounds 4 and 5 were submitted for acetylation with acetic anhydride, in the presence of pyridine and 4-dimethyilaminopiridine (DMAP), in order to obtain the compounds 3-acetyl-marinobufagin (7) and 3-acetyl-telocinobufogin (8). The isolated and derivatised compounds were identified by 1H and 13C NMR, and their molecular mass confirmed by mass spectrometry. All compounds (except 1 and 3) were tested in cytotoxic assays by the MTT method and presented cytotoxic potential against human cancer cell lines, as well as against non-tumoral human embryonic kidney HEK-293 cells. With the exception of compound 2, all molecules presented IC50 values < 4 µM, and none caused hemolysis of human erythrocytes, demonstrating a promising cytotoxic potential of natural and chemically-modified bufadienolides. This study presents a detailed contribution of bioactive chemicals from Brazilian Amazon Rhinella species, and indicates promising areas for further studies and pharmaceutical investments.


Assuntos
Venenos de Anfíbios/toxicidade , Bufo marinus , Animais , Brasil , Linhagem Celular Tumoral , Células HEK293 , Humanos , Peçonhas
3.
Bioorg Med Chem Lett ; 30(2): 126851, 2020 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-31836446

RESUMO

Quinoxaline derivatives are reported as antineoplastic agents against a variety of human cancer cell lines, with some compounds being submitted to clinical trials. In this work, we report the synthesis, characterization and cytotoxicity potential of a new series of quinoxalinyl-hydrazones. The most cytotoxic compound was (E)-2-[2-(2-pyridin-2-ylmethylene)hydrazinyl]quinoxaline (PJOV56) that presented a time-dependent effect against HCT-116 cells. After 48 h of incubation, PJOV56 was able to induce autophagy and apoptosis of HCT-116 cells, mediated by upregulation of Beclin 1, upregulation of LC3A/B II and activation of caspase 7. Apoptosis was induced along with G0/G1 cell cycle arrest at the highest concentration of PJOV56 (6.0 µM). Thus, PJOV56 showed a dose-dependent mode of action related to induction of autophagy and apoptosis in HCT-116 cells.


Assuntos
Apoptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Neoplasias Colorretais/tratamento farmacológico , Hidrazonas/síntese química , Quinoxalinas/síntese química , Humanos , Hidrazonas/química , Quinoxalinas/química , Relação Estrutura-Atividade
4.
Clinics (Sao Paulo) ; 73(suppl 1): e813s, 2018 12 10.
Artigo em Inglês | MEDLINE | ID: mdl-30540125

RESUMO

Cell cycle control genes are frequently mutated in cancer cells, which usually display higher rates of proliferation than normal cells. Dysregulated mitosis leads to genomic instability, which contributes to tumor progression and aggressiveness. Many drugs that disrupt mitosis have been studied because they induce cell cycle arrest and tumor cell death. These antitumor compounds are referred to as antimitotics. Vinca alkaloids and taxanes are natural products that target microtubules and inhibit mitosis, and their derivatives are among the most commonly used drugs in cancer therapy worldwide. However, severe adverse effects such as neuropathies are frequently observed during treatment with microtubule-targeting agents. Many efforts have been directed at developing improved antimitotics with increased specificity and decreased likelihood of inducing side effects. These new drugs generally target specific components of mitotic regulation that are mainly or exclusively expressed during cell division, such as kinases, motor proteins and multiprotein complexes. Such small molecules are now in preclinical studies and clinical trials, and many are products or derivatives from natural sources. In this review, we focused on the most promising targets for the development of antimitotics and discussed the advantages and disadvantages of these targets. We also highlighted the novel natural antimitotic agents under investigation by our research group, including combretastatins, withanolides and pterocarpans, which show the potential to circumvent the main issues in antimitotic therapy.


Assuntos
Antimitóticos/química , Antineoplásicos/química , Produtos Biológicos/química , Desenvolvimento de Medicamentos/métodos , Antimitóticos/farmacologia , Antineoplásicos/farmacologia , Produtos Biológicos/farmacologia , Humanos , Mitose/efeitos dos fármacos , Neoplasias/tratamento farmacológico , Neoplasias/patologia
5.
Toxicol Lett ; 285: 121-131, 2018 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-29287997

RESUMO

Skin toad secretion present physiologically active molecules to protect them against microorganisms, predators and infections. This work detailed the antiproliferative action of marinobufagin on tumor and normal lines, investigate its mechanism on HL-60 leukemia cells and its toxic effects on Allium cepa meristematic cells. Initially, cytotoxic action was assessed by colorimetric assays. Next, HL-60 cells were analyzed by morphological and flow cytometry techniques and growing A. cepa roots were examined after 72 h exposure. Marinobufagin presented high antiproliferative action against all human tumor lines [IC50 values ranging from 0.15 (leukemia) to 7.35 (larynx) µM] and it failed against human erythrocytes and murine lines. Human normal peripheral blood mononuclear cells (PBMC) were up to 72.5-fold less sensitive [IC50: 10.88 µM] to marinobufagin than HL-60 line, but DNA strand breaks were no detected. Leukemia treaded cells exhibited cell viability reduction, DNA fragmentation, phosphatidylserine externalization, binucleation, nuclear condensation and cytoplasmic vacuoles. Marinobufagin also reduced the growth of A. cepa roots (EC50: 7.5 µM) and mitotic index, caused cell cycle arrest and chromosomal alterations (micronuclei, delays and C-metaphases) in meristematic cells. So, to find out partially targeted natural molecules on human leukemia cells, like marinobufagin, is an amazing and stimulating way to continue the battle against cancer.


Assuntos
Antineoplásicos/farmacologia , Bufanolídeos/farmacologia , Ciclo Celular/efeitos dos fármacos , Quebras de DNA , Cebolas/efeitos dos fármacos , Adolescente , Adulto , Animais , Antineoplásicos/isolamento & purificação , Antineoplásicos/toxicidade , Bufanolídeos/isolamento & purificação , Bufanolídeos/toxicidade , Bufonidae/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Ensaio Cometa , Relação Dose-Resposta a Droga , Eritrócitos/efeitos dos fármacos , Células HL-60 , Voluntários Saudáveis , Hemólise/efeitos dos fármacos , Humanos , Leucócitos Mononucleares/efeitos dos fármacos , Meristema/citologia , Meristema/efeitos dos fármacos , Meristema/genética , Micronúcleos com Defeito Cromossômico/induzido quimicamente , Cebolas/citologia , Cebolas/genética , Pele/metabolismo , Adulto Jovem
6.
Clinics ; Clinics;73(supl.1): e813s, 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-974953

RESUMO

Cell cycle control genes are frequently mutated in cancer cells, which usually display higher rates of proliferation than normal cells. Dysregulated mitosis leads to genomic instability, which contributes to tumor progression and aggressiveness. Many drugs that disrupt mitosis have been studied because they induce cell cycle arrest and tumor cell death. These antitumor compounds are referred to as antimitotics. Vinca alkaloids and taxanes are natural products that target microtubules and inhibit mitosis, and their derivatives are among the most commonly used drugs in cancer therapy worldwide. However, severe adverse effects such as neuropathies are frequently observed during treatment with microtubule-targeting agents. Many efforts have been directed at developing improved antimitotics with increased specificity and decreased likelihood of inducing side effects. These new drugs generally target specific components of mitotic regulation that are mainly or exclusively expressed during cell division, such as kinases, motor proteins and multiprotein complexes. Such small molecules are now in preclinical studies and clinical trials, and many are products or derivatives from natural sources. In this review, we focused on the most promising targets for the development of antimitotics and discussed the advantages and disadvantages of these targets. We also highlighted the novel natural antimitotic agents under investigation by our research group, including combretastatins, withanolides and pterocarpans, which show the potential to circumvent the main issues in antimitotic therapy.


Assuntos
Humanos , Produtos Biológicos/química , Antimitóticos/química , Desenvolvimento de Medicamentos/métodos , Antineoplásicos/química , Produtos Biológicos/farmacologia , Antimitóticos/farmacologia , Mitose/efeitos dos fármacos , Neoplasias/patologia , Neoplasias/tratamento farmacológico , Antineoplásicos/farmacologia
7.
Fortaleza; s.n; 2016. 118 p.
Tese em Português | LILACS | ID: biblio-972023

RESUMO

As quinoxalinas são compostos heterocíclicos de baixo peso molecular, em sua maioria,sintéticos. Apresentam múltiplas atividades biológicas, o que justifica o crescente interesse daindústria farmacêutica. Entre essas atividades, pode-se citar a antineoplásica, antiinflamatória,antibacteriana, antiviral, antifúngica, antiparasitária, antidiabética, entre outras.O presente estudo avaliou os efeitos citotóxicos de três quinoxalinas sintéticas sobre aviabilidade de células tumorais. Por meio do método colorimétrico do MTT, as quinoxalinastestadas apresentaram CI50 decresente em função do tempo (24h, 48h e 72h). Dentre elas, aPJOV 56 destacou-se pela sua atividade antiproliferativa, com valores compreendidos entre0,82 a 7,35 µM (72 h) em células da linhagem HL-60 e HEPG2, respectivamente. Natentativa de caracterizar a ação citotóxica da molécula, a linhagem de câncer colorretal HCT-116 foi selecionada para tratamento com três concentrações (1,5, 3 e 6 µM) por 48h...


Assuntos
Humanos , Quinoxalinas , Neoplasias Colorretais , Compostos Heterocíclicos , Envelhecimento
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