RESUMO
Mimosa scabrella Benth., popularly known as ''bracatinga'', is a pioneer and endemic species of Brazil, occurring in Mixed Ombrophilous Forest associated with Brazilian Atlantic Rainforest biomes. It is a fast-growing tree of the Fabaceae family that facilitates the dynamics of ecological succession. SSR development, when there is no genome sequence, is time and labor intensive and there are no molecular markers for M. scabrella. We developed and validated the first microsatellite markers for this tetraploid species, evaluating mother trees and progenies. Using Illumina sequencing, we identified 290 SSR loci and 211 primer pairs. After 31 SSR loci PCR/agarose electrophoresis selection, a subset of 11 primer pairs was synthetized with fluorescence in the forward primer for PCR and capillary electrophoresis validation with leaf DNA of 33 adult and 411 progeny individuals. Polymorphic locus percentage was 36, 4 in 11 loci, 3 chloroplast SSRs, and 1 nuclear SSR. Allele number of polymorphic loci ranged from 2 to 11 alleles considering all sampling. All 11 primer pairs were also tested for cross-species amplification for five Fabaceae-Mimosoideae species, ranging from 2 loci transferred to Calliandra tweedii Benth. and all 11 loci transferred to Mimosa taimbensis Burkart. The assessed and validated SSR markers for M. scabrella are suitable and useful for analysis and population genetic studies.
Assuntos
Primers do DNA/síntese química , Repetições de Microssatélites , Mimosa/genética , Núcleo Celular/genética , Cloroplastos/genética , Marcadores Genéticos , Análise de Sequência de DNA/métodos , Especificidade da Espécie , TetraploidiaRESUMO
In this work, we analyse morpho-physiological modifications presented during the allomorphic growth of the aroid vine Rhodospatha oblongata Poepp throughout its ascent into the forest canopy. We test the hypothesis that morphological modifications in the root, shoot and leaf are followed by a gradual improvement of the xylem vascular system in order to increase water acquisition and transport as body size increases. The characterisation of these structural modifications was based on 30-35 specimens divided into six size classes. The dimensions of shoots, leaves and roots were quantified and qualified. The transition from the terrestrial to the epiphytic phase was followed by a simultaneous increase of leaf number and lamina area, together with increased length and diameter of the petiole. Furthermore, as the plant grows, the shoot internodes become shorter and thicker. However, occurrence of aerial roots is the most important characteristic in the ascending phase. In taller individuals, the increase in number of roots with wider xylem vessels guarantees an increased theoretical xylem hydraulic conductance for this growth phase. Along an acropetal direction of the same shoot, the diameter of xylem vessels increased, while the number of vessels per stele area decreased, in contrast with such allometric models as that of West, Brown and Enquist, showing that xylem vessel number and diameter taper in a reverse manner along the same direction. Such structural changes of R. oblongata allow improved foraging for light and water, facilitating the survival of bigger-sized plants of this vine in the canopy.
Assuntos
Araceae/fisiologia , Floresta Úmida , Araceae/crescimento & desenvolvimento , Brasil , Clima , Estruturas VegetaisRESUMO
In this study, the chemical composition and the in vitro schistosomicidal properties of the essential oil obtained from Bidens sulphurea flowers (Bs-EO) were investigated. Its major constituents were identified as being 2,6-di-tert-butyl-4-methylphenol (44.98%), germacrene D (33.70%) and ß-caryophyllene (10.23%). Bs-EO at 100 µg mL(-1) caused death of all the adult worms and promoted separation of the couple pairs into individual male and female within 48 h, besides leading to a significant decrease in the motility of the parasites. This oil was also responsible for a remarkable reduction in the number of eggs and the percentage of developed eggs produced by adult worms. These results suggest that the Bs-EO can be considered a promising source for the development of new schistosomicidal agents.
Assuntos
Asteraceae/química , Flores/química , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Esquistossomicidas/química , Esquistossomicidas/farmacologia , Animais , Sesquiterpenos Policíclicos , Sesquiterpenos/química , Sesquiterpenos/farmacologia , Sesquiterpenos de Germacrano/química , Sesquiterpenos de Germacrano/farmacologiaRESUMO
O objetivo deste trabalho foi levantar e identificar as espécies vegetais que ocorrem em áreas úmidas (banhados) do Planalto Catarinense e associá-las aos seus respectivos potenciais bioativos, validados em testes laboratoriais ou relatados em estudos etnobotânicos. O estudo foi realizado em 12 áreas de banhados localizadas em fazendas com reflorestamentos comerciais de espécies exóticas de propriedade da Empresa Klabin S.A. A amostragem da vegetação foi realizada por meio de transecção no sentido transversal na maior largura dos banhados e na área de transição destes com a área mais drenada (em faixa limitada de três metros), onde foram coletados os espécimes férteis a cada metro da transecção. Após a identificação das espécies foi realizada uma revisão bibliográfica sobre o potencial bioativo das mesmas junto à bases de dados científicos utilizando-se os trabalhos que atestam o potencial e também os trabalhos que valorizam o conhecimento de populações locais. Nas áreas amostradas foram identificadas 235 espécies classificadas em 40 famílias botânicas. Destas, 28 espécies, classificadas em 11 famílias, possuem potencial bioativo. Do total de espécies identificadas, 18 apresentaram potencial validado cientificamente e 10 são citadas quanto ao seu potencial em trabalhos de etnobotânica. Ainda é pouco conhecida a diversidade de banhados no Planalto Catarinense sendo necessário mais estudo para o conhecimento da flora local. O estudo mostrou um grande número de espécies com potencial validado e que poderiam ser utilizadas pela população, enquanto outras que merecem pesquisas complementares.
Our objective was to survey and identify the species that occur in wetlands of the uplands of the state of Santa Catarina and associate them to their bioactive potential, validated by laboratory tests or reported in ethnobotanical studies. The study was conducted in 12 wetland areas, located in farms with commercial reforestation of exotic species (these areas belong to the Klabin S.A. Company). The sampling of the vegetation was performed by transection in the transverse direction, in the widest portion of the wetlands and in the transition area to the drier portion (a limited strip of approximately 3 meters). Fertile specimens were collected at every meter of the transection. After the identification of the species, a literature review on the bioactive potential of these species was carried out on the scientific databases, using the studies that attest the potential value of the species and also studies that value the expertise from local populations. In the sampled areas, 235 species were identified and classified into 40 botanical families. From these, 28 species, classified into 11 families, have bioactive potential. Among all species identified, 18 showed scientifically accredited bioactive potential and 10 were cited as to their potential in ethnobotanical studies. Little is known about the diversity of the wetlands in the uplands of the state of Santa Catarina, and further studies are needed to increase the knowledge on the local flora. This study showed a large number of species with validated potential and that could be used by the population, while others have not yet been studied, but that are potential candidates for further researches.
Assuntos
Biodiversidade , Áreas Alagadas , Plantas Medicinais/efeitos adversos , Etnobotânica/instrumentaçãoRESUMO
O objetivo deste trabalho foi pesquisar dados químicos, biológicos e etnobotânicos na literatura científica de espécies medicinais de um remanescente de Floresta Ombrófila Mista Altomontana. A base para este estudo foi um levantamento florístico realizado na Fazenda das Nascentes, Urupema-SC entre agosto de 2007 e setembro de 2008. A partir da lista de espécies que resultou deste levantamento, foi realizada uma revisão bibliográfica sobre o potencial medicinal das espécies inventariadas. Para as espécies com dados de ação medicinal, foi elaborada chave de identificação vegetativa. Das 64 espécies listadas foram encontradas informações na bibliografia consultada sobre o potencial medicinal de 29. As principais familias foram Asteraceae (oito espécies) e Myrtaceae (três espécies). O hábito que mais se destacou entre as plantas com potencial medicinal foi o arbóreo (13 espécies). O componente químico de maior ocorrência entre as espécies foi o óleo essencial (60 por cento das espécies). As atividades terapêuticas mais citadas na literatura consultadas foram antimicrobiana, anti-oxidante, anti-inflamatória, antiviral, antifúngica e anestésica. Os resultados encontrados indicam o imenso potencial econômico da Floresta Ombrófila Mista e ambientes associados como fonte de recursos naturais que fazem parte da cultura e do patrimônio catarinense.
The aim of this work was to search for chemical, biological and ethnobotanical data in the scientific literature on medicinal species from a remnant of High Montane Araucaria Moist Forest. This study was based on the floristics performed in "Fazenda das Nascentes", Urupema Municipality, Santa Catarina State, Brazil between August 2007 and September 2008. From the list of species obtained in this survey, a review on the medicinal potential of these recorded species was done. A vegetative identification key was elaborated for species with medicinal action Information about medicinal potential was found in the researched bibliography for 29 off the 64 listed species. The main families were Asteraceae (eight species) and Myrtaceae (three species). The arboreal habit predominated among plants with medicinal potential (13 species). The most frequent chemical component among species was essential oil (60 percent species). The most cited therapeutic activities in the researched literature were antimicrobial, antioxidant, anti-inflammatory, antiviral, antifungal and anesthetic. The present results indicate the huge economic potential of Araucaria Moist Forest and associated environments as sources of natural resources that are part of the culture and inheritance from the Santa Catarina State.
Assuntos
Brasil , Traqueófitas , Plantas Medicinais/química , Árvores , Coleta de Dados/classificação , Etnobotânica/classificação , Etnobotânica/estatística & dados numéricosRESUMO
Drimys brasiliensis Miers, conhecida como cataia ou casca-de-anta, é árvore nativa da Mata Atlântica e as cascas tem sido utilizadas medicinalmente a partir da exploração de plantas em populações naturais. Este trabalho teve como objetivo estudar a demografia de duas populações naturais de D. brasiliensis, (1) Reserva Genética Florestal de Caçador (RGFC) e (2) Floresta Nacional de Caçador (FLONA), ambas no município de Caçador (SC), visando fundamentar estratégias de exploração sustentável e conservação deste recurso florestal não-madeireiro. A maior luminosidade no sub-bosque existente na FLONA atuou positivamente na espécie, aumentado a capacidade de incremento em diâmetro à altura do peito (DAP), altura (H) e área basal (AB), além de antecipar a reprodução em plantas de menor porte. A análise do padrão espacial das plantas reprodutivas na RGFC mostrou padrão agregado, e na FLONA até a distância de 25 metros. Esse comportamento indicou que a espécie apresenta potencial de manejo em florestas secundárias, formação sucessional em que se encontra a maioria dos remanescentes de Mata Atlântica, além de indicar potencial para cultivo em sistemas agroflorestais.
Known as "cataia" or "casca-de-anta", Drimys brasiliensis Miers is a tree native to the Atlantic Forest and its barks have been medicinally used by exploring natural populations. The present work aimed to study the demography of two D. brasiliensis natural populations, (1) Caçador Forest Genetic Reserve (RGFC) and(2) Caçador National Forest (FLONA), both located in Caçador Municipality, Santa Catarina State, Brazil, in order to establish strategies for sustainable exploration and conservation of such non-timber forest resource. The higher luminosity in FLONA understory was beneficial to the species by increasing their increment capability regarding diameter at breast height (DBH), height (H) and basal area (BA), and made the reproduction in smaller plants earlier. The spatial analysis of reproductive plants in RGFC had an aggregate pattern, whereas in FLONA until 25m distance was detected. Such results indicated that this species presents potential for management in secondary forests, the successional formation where the major Atlantic Forest remnants are found, as well as potential for cultivation in agroforestry systems.
Assuntos
Drimys/anatomia & histologia , Drimys/crescimento & desenvolvimento , Drimys/fisiologia , Winteraceae/anatomia & histologia , Winteraceae/crescimento & desenvolvimento , Brasil , Florestas , Comportamento Reprodutivo/fisiologia , Agricultura FlorestalRESUMO
The effect of different levels of progesterone (P4) concentrations on follicle growth and ovulatory capacity was evaluated in 40 crossbred Bos indicus x Bos taurus cyclic heifers submitted to distinct PGF2α + progesterone-based protocols. Heifers in CIDR_PGF8 group (n = 10) received 2.0 mg i.m. estradiol benzoate (EB) and a new controlled internal drug release containing 1.9 g of progesterone (CIDR) on day 0 of study. At the time of CIDR withdrawal (day 8), heifers received an i.m. injection of PGF2α and 24 h later a second EB i.m. injection (0.5 mg). The three other groups received EB injections and CIDR insertion/withdrawal as aforementioned, except that an i.m. injection of PGF2α was administered on day 5. In addition, heifers in the CIDR_PGF5 group (n = 10) received a new CIDR, while heifers in theCIDR1x_PGF5 (n = 9) and CIDR2x_PGF5 (n = 11) groups received a previously used CIDR for 8 and 14 days, respectively. Heifers that received a PGF2α injection on day 5 showed lower circulating P4 than heifers treated on day 8 (CIDR_PGF5 = 1.98 ± 0.21 ng/ml; CIDR1x_PGF5 = 1.69 ± 0.17 ng/ml and CIDR2x_PGF5 = 1.33 ± 0.08 ng/ml versus CIDR_PGF8 = 3.31 ± 0.45 ng/ml). The dominant follicle (DF) growth rate was slower in those heifers receiving PGF2α injection on day 8 (CIDR_PGF8 = 0.72 ± 0.13 mm/day) than groups treated on day 5 (CIDR_PGF5 = 0.96 ± 0.12 mm/day; CIDR1x_PGF5 = 1.06 ± 0.15 mm/day and CIDR2x_PGF5 = 1.01 ± 0.06mm/day). In consequence, preovulatory follicle diameter on day 10 was smaller in those animals injected on day 8 (CIDR_PGF8 = 8.81 ± 6.7 mm) than in those treated on day 5 (CIDR_PGF5 = 10.00 ± 0.58 mm CIDR1x_PGF5 = 10.5 ± 0.69 mm and CIDR2x_PGF5 = 10.5 ± 0.35 mm). For heifers receiving PGF2α injection on day 5, no significant differences on plasma P4 concentrations, folliculargrowth rate and DF diameters were observed among heifers that received new or previously used CIDR inserts. These results suggest that the presence of corpus luteum during synchronization protocols is the main factor responsible for the increase in the plasma P4 concentrations and inhibition of DF growth.(AU)
Assuntos
Animais , Progesterona/análise , Hormônio Liberador de Gonadotropina/análise , Bovinos/classificação , FisiologiaRESUMO
The effect of different levels of progesterone (P4) concentrations on follicle growth and ovulatory capacity was evaluated in 40 crossbred Bos indicus x Bos taurus cyclic heifers submitted to distinct PGF2α + progesterone-based protocols. Heifers in CIDR_PGF8 group (n = 10) received 2.0 mg i.m. estradiol benzoate (EB) and a new controlled internal drug release containing 1.9 g of progesterone (CIDR) on day 0 of study. At the time of CIDR withdrawal (day 8), heifers received an i.m. injection of PGF2α and 24 h later a second EB i.m. injection (0.5 mg). The three other groups received EB injections and CIDR insertion/withdrawal as aforementioned, except that an i.m. injection of PGF2α was administered on day 5. In addition, heifers in the CIDR_PGF5 group (n = 10) received a new CIDR, while heifers in theCIDR1x_PGF5 (n = 9) and CIDR2x_PGF5 (n = 11) groups received a previously used CIDR for 8 and 14 days, respectively. Heifers that received a PGF2α injection on day 5 showed lower circulating P4 than heifers treated on day 8 (CIDR_PGF5 = 1.98 ± 0.21 ng/ml; CIDR1x_PGF5 = 1.69 ± 0.17 ng/ml and CIDR2x_PGF5 = 1.33 ± 0.08 ng/ml versus CIDR_PGF8 = 3.31 ± 0.45 ng/ml). The dominant follicle (DF) growth rate was slower in those heifers receiving PGF2α injection on day 8 (CIDR_PGF8 = 0.72 ± 0.13 mm/day) than groups treated on day 5 (CIDR_PGF5 = 0.96 ± 0.12 mm/day; CIDR1x_PGF5 = 1.06 ± 0.15 mm/day and CIDR2x_PGF5 = 1.01 ± 0.06mm/day). In consequence, preovulatory follicle diameter on day 10 was smaller in those animals injected on day 8 (CIDR_PGF8 = 8.81 ± 6.7 mm) than in those treated on day 5 (CIDR_PGF5 = 10.00 ± 0.58 mm CIDR1x_PGF5 = 10.5 ± 0.69 mm and CIDR2x_PGF5 = 10.5 ± 0.35 mm). For heifers receiving PGF2α injection on day 5, no significant differences on plasma P4 concentrations, folliculargrowth rate and DF diameters were observed among heifers that received new or previously used CIDR inserts. These results suggest that the presence of corpus luteum during synchronization protocols is the main factor responsible for the increase in the plasma P4 concentrations and inhibition of DF growth.
Assuntos
Animais , Hormônio Liberador de Gonadotropina/análise , Progesterona/análise , Bovinos/classificação , FisiologiaRESUMO
Pentraxin 3 (PTX3) is a tumor necrosis factor and interleukin-1beta-stimulated gene that encodes a long PTX with proinflammatory activity. Here, we show that peritoneal macrophages derived from PTX3 transgenic (Tg) mice express higher levels of PTX3 mRNA than macrophages from wild-type (WT) mice, at basal level as well as upon stimulation with zymosan (Zy). Macrophages from Tg mice also showed improved opsonin-independent phagocytosis of Zy particles and the yeast form of the fungus Paracoccidioides brasiliensis. In the case of P. brasiliensis, an enhanced microbicidal activity accompanied by higher production of nitric oxide was also observed in macrophages from Tg mice. Using fluorescein-activated cell sorter analysis and reverse transcriptase-polymerase chain reaction, we demonstrated that basal level of Toll-like receptor-6 and Zy-induced dectin-1 expression was slightly but consistently higher in macrophages from Tg mice than in macrophages from WT mice. Recombinant (r)PTX3 protein binds to Zy particles as well as to yeast cells of P. brasiliensis and addition of rPTX3, to a culture of WT-derived macrophages containing Zy leads to an increase in the phagocytic index, which parallels that of Tg-derived macrophages, demonstrating the opsonin-like activity of PTX3. It is important that blockade of dectin-1 receptor inhibited the phagocytosis of Zy particles by WT and PTX3 Tg macrophages, pointing out the relevant role of dectin-1 as the main receptor involved in Zy uptake. Our results provide evidence for a role of PTX3 as an important component of the innate-immune response and as part of the host mechanisms that control fungal recognition and phagocytosis.
Assuntos
Proteína C-Reativa/genética , Macrófagos Peritoneais/metabolismo , Proteínas de Membrana/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Proteínas Opsonizantes/genética , Fagocitose/genética , Componente Amiloide P Sérico/genética , Zimosan/imunologia , Animais , Sítios de Ligação/efeitos dos fármacos , Sítios de Ligação/genética , Proteína C-Reativa/metabolismo , Feminino , Imunidade Inata/genética , Lectinas Tipo C , Macrófagos Peritoneais/efeitos dos fármacos , Macrófagos Peritoneais/imunologia , Masculino , Glicoproteínas de Membrana/efeitos dos fármacos , Glicoproteínas de Membrana/metabolismo , Proteínas de Membrana/antagonistas & inibidores , Camundongos , Camundongos Transgênicos , Proteínas do Tecido Nervoso/antagonistas & inibidores , Óxido Nítrico/metabolismo , Proteínas Opsonizantes/metabolismo , Paracoccidioides/imunologia , Fagocitose/efeitos dos fármacos , Fagocitose/imunologia , RNA Mensageiro/efeitos dos fármacos , RNA Mensageiro/metabolismo , Receptores de Superfície Celular/efeitos dos fármacos , Receptores de Superfície Celular/metabolismo , Proteínas Recombinantes de Fusão/metabolismo , Componente Amiloide P Sérico/metabolismo , Receptor 6 Toll-Like , Zimosan/metabolismo , Zimosan/farmacologiaRESUMO
In order to clearly visualize blood vessels, the monoclonal antibody (mAb) MEC 13.3 was used for an immunohistochemical staining on frozen sections of different mice mammary tumors. MEC 13.3 mAb is specific for endothelial cells (ECs) of mouse blood vessels and recognizes a molecule related to the murine form of CD31/PECAM. This mAb with immunoenzymatic technique or immunofluorescent labelling, was found to be a useful tool to quantify tumor neovascularization. Specifically, membrane reinforcement could be observed in vessel ECs, indicating the expression of CD31/ PECAM in their surface. The staining of ECs from tumors and from normal tissues was also compared. In this work, the use of MEC13.3 mAb is reported to recognize mice mammary tumor ECs as a useful tool to identify neovascularization. It would also be helpful for research on the origin and function of vascular endothelium in murine tumor experimental models.
Assuntos
Adenocarcinoma , Anticorpos Monoclonais , Endotélio Vascular/imunologia , Neoplasias Mamárias Experimentais , Animais , Especificidade de Anticorpos , Reações Antígeno-Anticorpo , Endotélio Vascular/química , Endotélio Vascular/citologia , Feminino , Imuno-Histoquímica , Glândulas Mamárias Animais/irrigação sanguínea , Glândulas Mamárias Animais/citologia , Camundongos , Camundongos Endogâmicos BALB C , Microcirculação , Células Tumorais CultivadasRESUMO
A stainless steel master die simulating a dental preparation for crown was used to make 3 gold castings from wax patterns obtained in 3 different ways: a) directly from the naked master die; b) from the same master die with a copper coping 30 microns thick; and c) with a similar copper coping, but 40 microns thick. The discrepancies of fit of the 3 master gold crowns were evaluated in 99 stones dies (33 for each casting) obtained from 11 different elastomeric impression materials (3 replicae of each). The results showed that: 1) stone dies with no coping presented a medium discrepancy of 284 microns, inadequate for clinical use; 2) the 30 microns and 40 microns copings presented medium discrepancies of 18 and 9 microns respectively, both suitable for clinical use. Thus, the use of copings tend to equalize the cervical discrepancy of fit of gold crowns, whatever be the elastomeric impression material used.
Assuntos
Coroas , Materiais para Moldagem Odontológica , Infiltração Dentária/prevenção & controle , Ligas de Ouro , Elastômeros de Silicone , Técnica de Moldagem Odontológica , Modelos DentáriosRESUMO
The following items are discussed: 1) Zoonoses with an urban cycle: a) Salmonellosis; b) Dermatophytozoonoses; c) Toxoplasmosis; d) Larva migrans visceralis; 3) Flea and tick infections; f) Zoonoses of cities located on lakes, rivers and the sea. 2) Animalization of the urban environment. 3) Zoonoses which may be transferred from the rural to the urban environment: a) Rural zoonoses transmitted to urban animals; b) Rural zoonoses transmitted to man in cities. 4) Zoonoses which may be transmitted from the urban to the rural environment.