RESUMO
OBJECTIVE(S): The aim of this study was to evaluate the orofacial manifestations in patients with Mobius syndrome (MS), establish an early adequate dental treatment and discuss the possible etiology of all cases examined based on information about the gestational intercurrences. DESIGN: Prospective study. SETTING: Special Care Dentistry Center, School of Dentistry, University of Sao Paulo, Brazil. Subject(s) and methods: Twenty-nine patients with MS aged 0 to 4 underwent prospective dental examination as well as early orthodontic treatment. RESULTS: All patients presented micrognathia, lack of lip seal, high arched palate and weak soft palate. The use of orthopedic appliances was recommended to all 29 patients, but only 13 adhered to treatment and were monitored for at least 24 months. We observed that, after 24 months of treatment, the palate was expanded and micrognathia became less severe in the majority of the cases. Pregnancy-related complications were reported by 27 (97%) of the 29 mothers. CONCLUSION(S): The early use of orthopedic appliances was important to prevent malocclusion and glossoptosis. Attempted abortion with misoprostol is associated with an increased risk of MS in infants.
Assuntos
Assistência Odontológica para Doentes Crônicos , Síndrome de Möbius , Ortodontia Preventiva , Abortivos não Esteroides/efeitos adversos , Pré-Escolar , Paralisia Facial/etiologia , Feminino , Humanos , Lactente , Masculino , Micrognatismo/etiologia , Micrognatismo/terapia , Misoprostol/efeitos adversos , Síndrome de Möbius/induzido quimicamente , Síndrome de Möbius/complicações , Palato Duro/anormalidades , Gravidez , Efeitos Tardios da Exposição Pré-Natal , Estudos Prospectivos , Língua/anormalidadesRESUMO
Epithelial-myoepithelial carcinoma of the salivary gland is a rare, low-grade, neoplasm, composed of ductal and myoepithelial cells. We present two novel cell lines, which have been characterised by immunofluorescence, derived from an epithelial-myoepithelial carcinoma of the parotid gland. A resected mass of the parotid gland was diagnosed as an epithelial-myoepithelial carcinoma by routine histological examination. Part of the specimen was labelled with a panel of antibodies confirming the tumour type. The other part was finely minced and the explants were incubated in DMEM supplemented with penicillin and streptomycin, at 37 degrees C in a humidified 5% CO(2) atmosphere. Two cell types were identified by immunofluorescence-a small cobblestone cell, positive for AE1/AE3 and p53, and a polyhedral cell, positive for vimentin, smooth muscle markers and S-100. Herein two cell lines are presented in order to open up possibilities of new studies and a discussion of the events that culminate in this bimodal neoplasm is also performed.
Assuntos
Carcinoma/patologia , Proteínas Nucleares , Neoplasias Parotídeas/patologia , Células Tumorais Cultivadas/patologia , Adulto , Carcinoma/química , Técnicas de Cultura de Células/métodos , Feminino , Humanos , Queratinas/análise , Proteínas de Neoplasias/análise , Neoplasias Parotídeas/química , Proteínas Proto-Oncogênicas/análise , Proteínas Proto-Oncogênicas c-mdm2 , Células Tumorais Cultivadas/química , Proteína Supressora de Tumor p53/análiseRESUMO
BACKGROUND: Integrins, heterodimeric transmembranic adhesion molecules composed of alpha and beta subunits, have been implicated in normal and neoplastic biological processes. We investigated the patterns of expression of integrins subunits beta 1, beta 3 and beta 4 in neoplasms derived from the terminal segment of salivary glands in order to understand their expression patterns in the different structures of these tumours. METHODS: Immunohistochemistry using 'catalized signal amplification' (CSA) detection system was applied to paraffin-embedded specimens of polymorphous low-grade adenocarcinoma, adenoid cystic carcinoma and acinic cell carcinoma. Normal salivary glands were used as controls. RESULTS: Immunoexpression of integrins showed that neoplastic structures of the tumours studied tend to mimic the patterns seen in the normal structures of salivary glands from which they are derived. Anaplastic cells were negative for all integrins. CONCLUSIONS: This study revealed detailed topography of integrins in malignant tumours derived from intercalated acinar segment of salivary gland and this might be useful in the diagnosis of these lesions, especially of fine-needle aspiration (FNA) products or small specimens derived form incisional biopsies.
Assuntos
Cadeias alfa de Integrinas/genética , Cadeias beta de Integrinas/genética , Neoplasias das Glândulas Salivares/patologia , Adenocarcinoma/metabolismo , Adenocarcinoma/patologia , Carcinoma de Células Acinares/metabolismo , Carcinoma de Células Acinares/patologia , Carcinoma Adenoide Cístico/metabolismo , Carcinoma Adenoide Cístico/patologia , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Imuno-Histoquímica , Neoplasias das Glândulas Salivares/metabolismoRESUMO
Hand-Schuller-Christian disease is a multifocal variant of eosinophilic granuloma, characterised by the classical triad of bony lesions, exophthalmos and diabetes insipidus. This case relates recurrent Langerhans' cell histiocytosis lesions presented as destruction of periodontal support associated with diabetes in a 10-year-old patient. Medical history suggests that the case represents a case of Hand-Schuller Christian disease.
Assuntos
Histiocitose de Células de Langerhans/diagnóstico , Doenças Mandibulares/diagnóstico , Perda do Osso Alveolar/etiologia , Criança , Diabetes Insípido/complicações , Diagnóstico Diferencial , Gengivite/etiologia , Histiocitose de Células de Langerhans/complicações , Humanos , Masculino , Doenças Mandibulares/complicações , Recidiva , Mobilidade Dentária/etiologiaRESUMO
Paracoccidioidomycosis is an important deep mycosis, endemic in some areas of the South American countryside, with great incidence in males bearing rural activities, being unusual in urban developed centres. Cell-mediated immunity is the main host defence against the P. brasiliensis, and HIV-positive patients have been increasingly affected by the disease, although only a few reports are available in the literature. We present a case of paracoccidiodomicosis in an HIV-positive female, with unusual clinical history and histopathological aspects.
Assuntos
Infecções Oportunistas Relacionadas com a AIDS/diagnóstico , Doenças da Gengiva/microbiologia , Soropositividade para HIV/complicações , Paracoccidioidomicose/diagnóstico , Adulto , Brasil , Feminino , HumanosRESUMO
Paracoccidioidomycosis is an important deep mycosis, endemic in some areas of the South American countryside, with great incidence in males bearing rural activities, being unusual in urban developed centres. Cellmediated immunity is the main host defence against the P. brasiliensis, and HIV-positive patients have been increasingly affected by the disease, although only a few reports are available in the literature. We present a case of paracoccidiodomicosis in an HIV-positive female, with unusual clinical history and histopathological aspects