RESUMO
Our purpose was to determine the blood amino acid concentration during insulin induced hypoglycemia (IIH) and examine if the administration of alanine or glutamine could help glycemia recovery in fasted rats. IIH was obtained by an intraperitoneal injection of regular insulin (1.0 U/kg). The blood levels of the majority of amino acids, including alanine and glutamine were decreased (P < 0.05) during IIH and this change correlates well with the duration than the intensity of hypoglycemia. On the other hand, the oral and intraperitoneal administration of alanine (100 mg/kg) or glutamine (100 mg/kg) accelerates glucose recovery. This effect was partly at least consequence of the increased capacity of the livers from IIH group to produce glucose from alanine and glutamine. It was concluded that the blood amino acids availability during IIH, particularly alanine and glutamine, play a pivotal role in recovery from hypoglycemia.
Assuntos
Alanina/sangue , Glicemia/biossíntese , Gluconeogênese/efeitos dos fármacos , Glutamina/sangue , Hipoglicemia/sangue , Insulina/farmacologia , Fígado/efeitos dos fármacos , Aminoácidos/sangue , Animais , Glicemia/análise , Combinação de Medicamentos , Hipoglicemia/induzido quimicamente , Injeções Intraperitoneais , Fígado/metabolismo , Masculino , Ratos , Ratos WistarRESUMO
This report deals with the process of improving the stability of medfly, Ceratitis capitata, genetic sexing strains (GSS) based on the sw mutation on chromosome 2. This gene affects the rate of development as well as the eye colour and iridescence. The improved sexing strains were produced by mapping sw with deletions and then inducing and screening for new translocations with breakpoints close to the marker. The stability was assessed in large populations over many generations. Twenty-two new Y-2 translocations were identified and polytene chromosome analysis was performed to locate breakpoints. The translocation strains were ranked according to the distance of their breakpoints from sw. The map position of sw is region 20D on 2R. As data on the stability of the 22 strains accumulated, Cast 191 was shown to be the most promising as no recombination between sw and the male sex was found. After rearing the strain for 22 generations under semi-mass rearing conditions, with a population size of 15,000 adults and scoring 1000 flies per generation, only one such event was detected (estimated frequency = 3.1 x 10(-6)). Further tests are being carried out with this strain to assess its suitability as a genetic sexing strain for medfly Sterile insect technique (SIT).
Assuntos
Ceratitis capitata/genética , Genes de Insetos , Pré-Seleção do Sexo/métodos , Animais , Cruzamento , Ceratitis capitata/efeitos da radiação , Bandeamento Cromossômico , Quebra Cromossômica , Mapeamento Cromossômico , Cromossomos/genética , Cromossomos/efeitos da radiação , Cromossomos/ultraestrutura , Feminino , Marcadores Genéticos , Infertilidade Masculina/genética , Masculino , Controle Biológico de Vetores/métodos , Deleção de Sequência , Translocação Genética/genética , Cromossomo Y/genética , Cromossomo Y/ultraestruturaRESUMO
A random amplified polymorphic DNA polymerase chain reaction method using 10-base long commercial random primers was used to identify polymorphisms in Argentine populations of 2 fruit fly pests. A fast and reliable discrimination between Mediterranean fruit fly, Ceratitis capitata (Wiedemann), and Anastrepha fraterculus (Wiedemann) immature or mature stages was obtained using as few as 1 insect per assay. The population of origin of immature individuals can be identified unambiguously using 2 primers.
Assuntos
Dípteros/genética , Técnica de Amplificação ao Acaso de DNA Polimórfico , Animais , Dípteros/classificaçãoRESUMO
The redundant nerve roots syndrome is the association of high grade extradural lumbar spinal block with a tortuous elongated nerve roots depicted as multiple serpentiginous filling defects on myelography. A case of a 64-years-old man is presented. Laminectomy and facetectomy were done with great improvement of the symptoms. A discussion of this syndrome is presented.