RESUMO
The aim of this work was to study the efficiency of microcosms-scale floating treatment wetlands (FTWs) in the N and P removal from a synthetic runoff effluent and to evaluate the effluent tolerance of Typha domingensis. Each FTW consisted of a raft constructed with a plastic net where T. domingensis plants were installed. In order to evaluate the plant role, reactors with FTWs and without FTWs (controls) were used. P and N additions were carried out as follows: 5â¯mgâ¯L-1 P (P5 and P5-control); 10â¯mgâ¯L-1â¯N (N10 and N10-control); 5â¯mgâ¯L-1 Pâ¯+â¯10â¯mgâ¯L-1â¯N (P5N10 and P5N10-control). Also, a biological control (B-control) without contaminant addition was used. The removal of soluble reactive phosphorus and total phosphorus were significantly higher in the FTWs than in the controls. Ammonium and nitrate concentrations were not significantly different between FTWs and controls at the end of the experiment. However, nitrate concentrations showed significant differences between FTWs and controls during the experiment. N and P were mainly accumulated in plant tissues and not in the sediment. Plants tolerated the effluent conditions and showed a positive growth rate. The use of FTWs is a promising strategy for the sustainable treatment of water bodies affected by runoff waters.
Assuntos
Nitrogênio/análise , Fósforo/análise , Typhaceae/metabolismo , Eliminação de Resíduos Líquidos/métodos , Poluentes Químicos da Água/análise , Biodegradação Ambiental , Áreas AlagadasRESUMO
The aim of this work was to compare Cr(III) and Cr(VI) removal kinetics from water by Pistia stratiotes and Salvinia herzogii. The accumulation in plant tissues and the effects of both Cr forms on plant growth were also evaluated. Plants were exposed to 2 and 6 mg L(-1) of Cr(III) or Cr(VI) during 30 days. At the end of the experiment, Cr(VI) removal percentages were significantly lower than those obtained for Cr(III) for both macrophytes. Cr(III) removal kinetics involved a fast and a slow component. The fast component was primarily responsible for Cr(III) removal while Cr(VI) removal kinetics involved only a slow process. Cr accumulated principally in the roots. In the Cr(VI) treatments a higher translocation from roots to aerial parts than in Cr(III) treatments was observed. Both macrophytes demonstrated a high ability to remove Cr(III) but not Cr(VI). Cr(III) inhibited the growth at the highest studied concentration of both macrophytes while Cr(VI) caused senescence. These results have important implications in the use of constructed wetlands for secondary industrial wastewater treatment. Common primary treatments of effluents containing Cr(VI) consists in its reduction to Cr(III). Cr(III) concentrations in these effluents are normally below the highest studied concentrations in this work.
Assuntos
Araceae/metabolismo , Cromo/metabolismo , Gleiquênias/metabolismo , Poluentes Químicos da Água/metabolismo , Araceae/química , Biodegradação Ambiental , Cromo/química , Gleiquênias/química , Cinética , Raízes de Plantas/química , Raízes de Plantas/metabolismo , Poluentes Químicos da Água/químicaRESUMO
A free water surface constructed wetland (CW) designed for effluent treatment was dominated by the emergent macrophyte Typha domingensis reaching a cover of roughly 80% for 5 years. Highly efficient metal and nutrient removal was reported during this period. In June 2009, a population of approximately 30 capybaras (Hydrochoerus hydrochaeris) caused the complete depredation of the aerial parts of macrophytes. However, plant roots and rhizomes were not damaged. After depredation stopped, T. domingensis showed a luxuriant growth, reaching a cover of 60% in 30 days. The objective of this work was to evaluate the sustainability of the CW subjected to an extreme event. Removal efficiency of the system was compared during normal operation, during the depredation event and over the subsequent recovery period. The CW efficiently retained contaminants during all the periods studied. However, the best efficiencies were registered during the normal operation period. There were no significant differences between the performances of the CW over the last two periods, except for BOD. The mean removal percentages during normal operation/depredation event/recovery period, were: 84.9/73.2/74.7% Cr; 66.7/48.0/51.2% Ni; 97.2/91.0/89.4% Fe; 50.0/46.8/49.5% Zn; 81.0/84.0/80.4% NO3(-); 98.4/93.4/84.1% NO2(-); 73.9/28.2/53.2% BOD and 75.4/40.9/44.6% COD. SRP and TP presented low removal efficiencies. Despite the anoxic conditions, contaminants were not released from sediment, accumulating in fractions that proved to be stable faced with changes in the operating conditions of the CW. T. domingensis showed an excellent growth response, consequently the period without aerial parts lasted a few months and the CW could recover its normal operation. Plants continued retaining contaminants in their roots and the sediment increased its retention capacity, balancing the operating capacity of the system. This was probably due to the fact that the CW had reached its maturity, with a complete root-rhizome development. These results demonstrated that faced with an incidental problem, this mature CW was capable of maintaining its efficiency and recovering its vegetation, demonstrating the robustness of these treatment systems.
Assuntos
Typhaceae/crescimento & desenvolvimento , Eliminação de Resíduos Líquidos/métodos , Áreas Alagadas , Animais , Argentina , Biodegradação Ambiental , Análise da Demanda Biológica de Oxigênio , Sedimentos Geológicos , Metais/metabolismo , Nitratos/metabolismo , Fósforo/metabolismo , Raízes de Plantas/crescimento & desenvolvimento , Roedores , Poluentes Químicos da Água/metabolismoRESUMO
The aim of this work was to study the accumulation and fractionation of P in the inlet and outlet sediment of a constructed wetland for the wastewater treatment of a metallurgic plant in Argentina. It was important to predict whether P could be released into the water again by changing environmental conditions or retained over time. P-fractionation was performed using a sequential extraction method. Sediment cores were sliced at depths of: 0-3; 3-7 and 7-10 cm. Sediment showed high pH values and anoxic conditions. In the inlet area, P was principally bound to the carbonate fraction, whereas in the outlet area, it was mainly bound in the residual fraction. This behavior was justified by the effluent composition, which is rich in Ca2+ and Fe3+ and presents high values of pH and conductivity. These conditions favor CaCO3 and Fe(OOH)n precipitation and the subsequent sorption of P to their surface. The sediment active layer involved in the exchange reactions was the superficial one (0-3 cm). The wetland is highly efficient in P retention. P was retained by sediment in fractions that will not release it to the water while chemical and environmental conditions of the system are maintained.
Assuntos
Sedimentos Geológicos/análise , Fósforo/análise , Gerenciamento de Resíduos , Áreas Alagadas , Resíduos Industriais , MetalurgiaRESUMO
The aim of this work was to assess the uptake efficiencies, the uptake and bioaccumulation kinetics and the toxic effects of Cr, Ni and Zn on Eichhornia crassipes. Plants were exposed to 1 mg L(-1) of each metal and sampled during 30 days. E. crassipes removed 81%, 95% and 70% of Cr, Ni and Zn, respectively. Metal removal from water involved a fast and a slow component. Metals were accumulated fundamentally by roots. Cr was scarcely translocated to aerial parts. In these tissues, Ni showed the highest accumulation amount while Zn presented the highest accumulation rate. Metal toxicity on the biomass was different among treatments. However, biomass did not decrease in any case. All the studied metals produced chlorophyll decrease. The root cross-sectional area (CSA) and vessel number increased and the root length decreased when plants were exposed to Zn. Despite the toxic effects, E. crassipes accumulated Cr, Ni and Zn efficiently.
Assuntos
Eichhornia/efeitos dos fármacos , Metais Pesados/farmacologia , Raízes de Plantas/metabolismo , Clorofila/biossíntese , Cromo/farmacologia , Eichhornia/crescimento & desenvolvimento , Cinética , Metais Pesados/farmacocinética , Níquel/farmacologia , Raízes de Plantas/efeitos dos fármacos , Zinco/farmacologiaRESUMO
The aim of this work was to compare the adaptability of two different populations of Typha domingensis exposed to high pH and salinity. The plants were sampled from an uncontaminated natural wetland (NW) and a constructed wetland (CW) for the treatment of an industrial effluent with high pH and salinity. The plants from each population were exposed to the following combined treatments of salinity (mg l(-1)) and pH: 8,000/10 (values found in the CW); 8,000/7; 200/10 and 200/7 (typical values found in the NW). Chlorophyll concentration, relative growth rates (RGR) and root structure parameters (cross-sectional areas of root, stele and metaxylem vessels) were measured. Images of roots and leaves by scanning electronic microscopy (SEM) were obtained, and X-ray microanalysis in different tissues was carried out. In all treatments, the RGR and chlorophyll increase were significantly lower in the plants from the NW than in the plants from the CW. However, stress was observed when the plants from the CW were exposed to treatment 200/7. In treatment 8,000/10 the tissues of the plants from the NW showed severe damages. The root structure of plants from the CW was modified by salinity, while pH did not produce changes. In plants from the CW there were no differences between Na concentration in leaves of the treatments 8,000/10 and 200/7, indicating that Na was not transported to leaves. The CW population already possesses physiological and morphological adaptations due to the extreme conditions of pH and salinity. Because of its adaptive capacity, T. domingensis is an efficient species to treat wastewater of high pH and salinity.
Assuntos
Adaptação Fisiológica , Typhaceae/fisiologia , Biodegradação Ambiental , Clorofila/metabolismo , Água Doce/química , Concentração de Íons de Hidrogênio , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/metabolismo , Raízes de Plantas/fisiologia , Salinidade , Typhaceae/crescimento & desenvolvimento , Typhaceae/metabolismo , Áreas AlagadasRESUMO
The effects of Cr, Ni, Zn, and P exposure on the root anatomic structure, growth, and chlorophyll a concentration of Pistia stratiotes L. were studied. Plastic aquaria containing 50 g of wet plants and 5 L of pond water added with the contaminant(s) were disposed. The treatments were: (1) Cr, (2) Ni, (3) Zn, (4) P, (5) Cr + Ni + Zn, (6) Cr + Ni + Zn + P, and (7) control. Contaminant additions were done seven times. In each addition, concentrations of 1 mg of metals or 5 mg of P per liter of water were added. Chlorophyll a was an indicator more sensitive to Zn and Cr toxicity than the relative growth rate. Ni and Cr + Ni + Zn treatments were the most toxic ones, in which biomass and the root anatomical parameters (root length, cross-sectional areas [CSAs] of root, stele, and metaxylem vessels) decreased significantly. The addition of P to the treatment with combined metals attenuated the decrease in plant growth and root length, and caused a significant increase in CSAs of total metaxylem vessels, suggesting that P increased the tolerance of P. stratiotes to metals. This fact has important implications for the use of this macrophyte in constructed wetlands for industrial wastewater treatment.
Assuntos
Araceae/efeitos dos fármacos , Metais Pesados/toxicidade , Fósforo/toxicidade , Poluentes Químicos da Água/toxicidade , Araceae/anatomia & histologia , Araceae/crescimento & desenvolvimento , Araceae/metabolismo , Clorofila/análise , Clorofila A , Cromo/toxicidade , Metais Pesados/metabolismo , Níquel/toxicidade , Zinco/toxicidadeRESUMO
Typha domingensis had become the dominant species after 2 years of operation of a wetland constructed for metallurgical effluent treatment. Therefore, the main purpose of this study was to investigate its ability to tolerate the effluent and to maintain the contaminant removal efficiency of the constructed wetland. Plant, sediment, and water at the inlet and outlet of the constructed wetland and in two natural wetlands were sampled. Metal concentration (Cr, Ni, and Zn) and total phosphorus were significantly higher in tissues of plants growing at the inlet in comparison with those from the outlet and natural wetlands. Even though the chlorophyll concentration was sensitive to effluent toxicity, biomass and plant height at the inlet and outlet were significantly higher than those in the natural wetlands. The highest root and stele cross-sectional areas, number of vessels, and biomass registered in inlet plants promoted the uptake, transport, and accumulation of contaminants in tissues. The modifications recorded accounted for the adaptability of T. domingensis to the conditions prevailing in the constructed wetland, which allowed this plant to become the dominant species and enabled the wetland to maintain a high contaminant retention capacity.
Assuntos
Resíduos Industriais , Metais Pesados/toxicidade , Typhaceae/efeitos dos fármacos , Eliminação de Resíduos Líquidos , Poluentes Químicos da Água/toxicidade , Áreas Alagadas , Typhaceae/anatomia & histologiaRESUMO
The aim of the research was to assess the uptake efficiencies of Ni and P, their distribution in tissues along time and their toxic effects on the internal and external morphologies of Eichhornia crassipes. Aquaria with plants exposed to 1 mg Ni l(-1) or 5 mg P l(-1) and control were arranged in triplicate. Water and plants (aerial parts and roots) were sampled along 30 days. Ni uptake and tissue bioaccumulation kinetics was significantly faster than that of P. Mean root length, number of leaves, biomass and chlorophyll concentration were negatively affected by Ni, while these parameters were significantly increased by P in comparison with the control. Stele and metaxylem vessel cross-sectional areas (CSA) in the P treatment were significantly lower in comparison with that obtained in the Ni treatment and in control. Metaxylem vessels CSA in plants exposed to Ni were significantly higher while the number of vessels was significantly lower than those obtained in the control. Despite the toxic effects, E. crassipes efficiently accumulated Ni, probably due to the morphological plasticity of its root system.
Assuntos
Eichhornia/metabolismo , Níquel/metabolismo , Fósforo/metabolismo , Poluentes Químicos da Água/metabolismo , Adsorção , Argentina , Biodegradação Ambiental , Clorofila/metabolismo , Eichhornia/efeitos dos fármacos , Eichhornia/crescimento & desenvolvimento , Cinética , Níquel/farmacologia , Fósforo/farmacologia , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/metabolismo , Poluentes Químicos da Água/farmacologia , Purificação da Água , Áreas AlagadasRESUMO
A free water surface wetland was built to treat wastewater containing metals (Cr, Ni, Zn) and nutrients from a tool factory in Argentina. Water, sediment and macrophytes were sampled in the inlet and outlet area of the constructed wetland during three years. Three successive phases of vegetation dominance were developed and three different patterns of contaminant retention were observed. During the Eichhornia crassipes dominance, contaminants were retained in the macrophyte biomass; during the E. crassipes+Typha domingensis stage, contaminants were retained in the sediment and in the T. domingensis dominance stage, contaminants were retained in sediment and in the macrophyte biomass. Removal efficiency was not significantly different among the three vegetation stages, except for NH(4)(+) and i-P(diss). Because of its highest tolerance, T. domingensis is the best choice to treat wastewater of high pH and conductivity with heavy metals, a common result from many industrial processes.
Assuntos
Metais Pesados/isolamento & purificação , Fenômenos Fisiológicos Vegetais , Poluentes Químicos da Água/análise , Argentina , Eichhornia/crescimento & desenvolvimento , Condutividade Elétrica , Sedimentos Geológicos/microbiologia , Concentração de Íons de Hidrogênio , Resíduos Industriais/análise , Compostos Inorgânicos/isolamento & purificação , Typhaceae/crescimento & desenvolvimento , Áreas AlagadasRESUMO
A free water surface wetland was built to treat wastewater containing metals (Cr, Ni and Zn) and nutrients from a tool factory in Santo Tomé, Santa Fe, Argentina. Eichhornia crassipes became dominant and covered about 80% of the surface throughout the first year, and decreased progressively until its disappearance. When water depth was lowered Typha domingensis steadily increased plant cover and attained 30% of the surface by the end of the study. While E. crassipes was dominant, the wetland retained 62% of the incoming Cr and 48% of the Ni. NO3- and NO2-, were also removed (65% and 78%, respectively), while dissolved inorganic phosphate (i-P(diss)) and NH4+ were not removed. Zn was below 50 microg l(-1) in both the influent and effluent. Metal concentration in the sediments did not increase and retention was mediated through macrophytes uptake. During the period of E. crassipes decline the wetland retained 49% of the incoming Cr, 45% of Ni, 58% NO3-, 94% NO2-, 58% NH4+ and 47% i-P(diss). Cr, Ni and Zn in the bottom sediment increased in the inlet but not in the outlet. Since T. domingensis became dominant, retention was 58% Cr, 48% Ni and 64% i-P(diss), while 79% NO3-, 84% NO2- and 13% NH4+ were removed. Metals in the bottom sediment increased in the inlet. In spite of the significant growth of E. crassipes at the beginning, T. domingensis remained after most of the transplanted macrophytes had disappeared. Macrophyte disappearance could be related to the overall toxicity of several environmental constrains as high pH and conductivity, metal concentration, and sulphide presence.
Assuntos
Biodegradação Ambiental , Eichhornia/metabolismo , Metais Pesados/metabolismo , Typhaceae/metabolismo , Áreas Alagadas , Argentina , Eichhornia/crescimento & desenvolvimento , Resíduos Industriais , Solo/análise , Typhaceae/crescimento & desenvolvimentoRESUMO
This study was aimed at determining the spatial and temporal variation of P distribution in sediment of an artificial wetland for sewage and industrial wastewater treatment, and the fraction that is potentially involved in the P exchange processes. Influent, effluent, macrophytes and sediment at the inlet, middle and outlet areas were sampled over 24 months. The P-fractionation in sediment was performed following the EDTA method. Eichhornia crassipes and Typha domingensis removed P efficiently when cover was high, but E. crassipes caused anoxic conditions. The increase in cover of T. domingensis may contribute to attain oxic conditions and to improve P removal. When macrophytes are not present or when cover is low, sediment seems to increase removal efficiency. A significant increase in the concentration of the fraction of Fe(OOH) approximately P and mainly that of CaCO(3) approximately P can be observed at the inlet. High pH, Ca(2+) and CO(3)(-)(2) concentrations in the influent suggest that P co-precipitates together with CaCO(3). Therefore, it seems that CaCO(3) approximately P represents the main precipitating mechanism. However, mineralization of organic matter maintained the sediment at a pH range lower than the high values prevailing in the influent. CO(3)(-)(2) could undergo partial dissolution and the released i-P(diss) could be readsorbed onto the Fe(OOH) approximately P fraction. Since the environment for P retention (high pH, Fe, Ca and ionic concentrations) is largely provided by the influent, the wetland may be expected to continue retaining P as far as the composition of the influent is maintained and there are available adsorption sites in the sediment.
Assuntos
Sedimentos Geológicos/química , Compostos de Fósforo/análise , Eliminação de Resíduos Líquidos/métodos , Poluentes Químicos da Água/metabolismo , Purificação da Água/métodos , Áreas Alagadas , Adsorção , Argentina , Biodegradação Ambiental , Biomassa , Compostos de Fósforo/metabolismo , Fatores de Tempo , Água/química , Microbiologia da Água , Movimentos da Água , Poluentes Químicos da Água/análiseRESUMO
The aim of this work was to determine chromium and cadmium bioaccumulation processes of two free-floating macrophytes commonly used in wetlands for water treatment: Salvinia herzogii and Pistia stratiotes. Metal removal from the solution involves two stages: a fast one and a slow one. The fast stage of the Cd uptake is significantly different for each species, while it is not significantly different in Cr uptake. The most important processes of Cd uptake are biological ones in S. herzogii and adsorption, chelation and ionic exchange are in P. stratiotes. The main processes of Cr uptake in both macrophytes are adsorption, chelation and ion exchange. The slow stage is different for each species and metal. Cr precipitation induced by roots occurs in P. stratiotes. Cr uptake through leaves is probably the main cause of the increase of Cr in the aerial parts of S. herzogii.
Assuntos
Araceae/metabolismo , Cádmio/farmacocinética , Cromo/farmacocinética , Gleiquênias/metabolismo , Poluentes Químicos da Água/farmacocinética , Adsorção , Troca Iônica , Folhas de Planta/metabolismo , Raízes de Plantas/metabolismo , Especificidade da EspécieRESUMO
A pilot-scale wetland was constructed to assess the feasibility of treating the wastewater from a tool industry in Santo Tomé, Santa Fe, Argentina. The wastewater had high conductivity and pH, and contained Cr, Ni and Zn. This paper describes the growth of vegetation in the experimental wetland and the nutrient and metal removal. The wetland was 6 x 3 x 0.4 m. Water discharge was 1000 l d(-1) and residence time was 7d. After the wetland was rendered impermeable, macrophytes from Middle Paraná River floodplain were transplanted. Influent and effluent quality was analyzed every 15 d. TP, Cr, Ni and Zn concentrations in leaves, roots and sediment (inlet and outlet) were measured monthly. Cover and biomass of predominant species were estimated. Also, greenhouse experiments were carried out to measure the effects of conductivity and pH on floating species. The variables measured in the influent were significantly higher than those in the effluent, except for HCO(3)(-) and NH(4)(+). TP and metal concentrations in sediment at the inlet were significantly higher than those at the outlet. Conductivity and pH of the incoming wastewater were toxic for the floating species. Typha domingensis displaced the other species and reached positive relative cover rate and biomass greater than those at the undisturbed natural environment. T. domingensis proved to be highly efficient for the treatment of wastewater. For that reason, it is the advisable species for the treatment of wastewater of high conductivity and pH enriched with metals, characteristic of many industrial processes.
Assuntos
Cromo/metabolismo , Magnoliopsida/metabolismo , Níquel/metabolismo , Eliminação de Resíduos Líquidos/métodos , Zinco/metabolismo , Biodegradação Ambiental , Cromo/análise , Cromo/toxicidade , Concentração de Íons de Hidrogênio , Resíduos Industriais , Magnoliopsida/efeitos dos fármacos , Magnoliopsida/crescimento & desenvolvimento , Metalurgia , Níquel/análise , Níquel/toxicidade , Fósforo/análise , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/metabolismo , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/metabolismo , Poluentes Químicos da Água/toxicidade , Purificação da Água/métodos , Zinco/análise , Zinco/toxicidadeRESUMO
El objetivo del trabajo fue evaluar la capacidad de un humedal construído a escala piloto en la remoción de contaminantes de un efluente de alta salinidad de una industria metalúrgica. Los principales contaminantes son industriales y cloacales, y el efluente recibió un tratamiento primario previo. Se dispusieron plantas acuáticas regionales enraizadas y flotantes, y se determinó semanalmente la calidad química del efluente de entrada y de salida, siendo el funcionamiento del humedal experimental altamente satisfactorio