RESUMO
Background: : : : In livestock animals, which artificial insemination (AI) is widely spread, a healthy uterine environment is an important factor that should be taken into account for an adequate fertilization and establishment and development of pregnancy. The main limitation to spread the frozen semen insemination in sheep is the high costs of the laparoscopy AI and the difficulty of traversing the cervix. To overcome problems associated with transcervical artificial insemination, new techniques and instruments have been proposed. The manipulation of cervix during transcervical artificial insemination in sheep can lead to a local inflammatory reaction that releases inflammatory mediators, such as prostaglandins and cytokines, that either can negatively affect the uterine environment and consequently, the conception. In this regard, the injection of a non-steroidal anti-inflammatory (NSAI) at the moment of AI could reduce the secretion of inflammatory mediators, promoting a healthier uterine environment favorable to conception. The flunixin meglumine is a NSAI which reduces the synthesis of PGF2á by the inibition of the cyclooxigenase. The objective of this study was to evaluate the effect of flunixin meglumine (non-steroid antiinflammatory) injection on pregnancy rate of ewes inseminated by the transcervical technique. Materials, Methods & Results: In this experiment 6
RESUMO
Background: : : : In livestock animals, which artificial insemination (AI) is widely spread, a healthy uterine environment is an important factor that should be taken into account for an adequate fertilization and establishment and development of pregnancy. The main limitation to spread the frozen semen insemination in sheep is the high costs of the laparoscopy AI and the difficulty of traversing the cervix. To overcome problems associated with transcervical artificial insemination, new techniques and instruments have been proposed. The manipulation of cervix during transcervical artificial insemination in sheep can lead to a local inflammatory reaction that releases inflammatory mediators, such as prostaglandins and cytokines, that either can negatively affect the uterine environment and consequently, the conception. In this regard, the injection of a non-steroidal anti-inflammatory (NSAI) at the moment of AI could reduce the secretion of inflammatory mediators, promoting a healthier uterine environment favorable to conception. The flunixin meglumine is a NSAI which reduces the synthesis of PGF2á by the inibition of the cyclooxigenase. The objective of this study was to evaluate the effect of flunixin meglumine (non-steroid antiinflammatory) injection on pregnancy rate of ewes inseminated by the transcervical technique. Materials, Methods & Results: In this experiment 6
RESUMO
The aim of this study was to evaluate the use of a prostaglandin F2 analogue (PGF2) and oestradiol benzo-ate (ODB) in estrus synchronization programs. A hundred and eighty five non-lactating females divided in 4 groups were used. G1 (44) and G2 (46) received two applications of PGF2 (D0 and D11) and G3 (26) and G4 (37) received one application after 5 days of estrous detection. G1 and G3 also received a 1 mg ODB application 48 hours after the PGF2. The pregnancy rate at AI was 42,2% for G1, 47,8% for G2, 32,1% for G3 e 57,6% for G4 (p>0,05) and of 38,4% and 51,4% for animals submitted to FTAI and estrous detection, respectively (p>0,05). This results show that pregnancy rates of females fixed time inseminated didnt differ of that inseminated after estrus detection. Key-words: Beef cattle, estrus synchronization, fixed time artificial insemination, prostaglandin.
O objetivo deste estudo foi avaliar o emprego de um análogo da prostaglandina F2 (PGF2) em associação ao benzoato de estradiol (BE) em programas de sincronização de cios. Utilizaram-se 185 fêmeas não-lactantes divididas em quatro grupos. O G1 (44) e o G2 (46) receberam duas aplicações de PGF2 (D0 e D11) e os animais do G3 (26) e G4 (37) uma aplicação de PGF2, após cinco dias de detecção de cio. O G1 e o G3 receberam 1mg de BE 48 horas após a PGF2. A taxa de prenhez na IA foi de 42,2% no G1, 47,8% no G2, 32,1% no G3 e 57,6% no G4 (p>0,05) e de 38,4% e 51,4% para animais submetidos à IATF e à detecção de cio, respectivamente (p>0,05). Notou-se que a taxa de prenhez nas fêmeas inseminadas em tempo-fixo não diferiu daquelas que foram inseminadas após a detecção de cio. Palavras-chaves: Bovinos de corte, inseminação artificial em tempo fixo, prostaglandina, sincronização de cios.
RESUMO
The aim of this study was to evaluate the use of a prostaglandin F2 analogue (PGF2) and oestradiol benzo-ate (ODB) in estrus synchronization programs. A hundred and eighty five non-lactating females divided in 4 groups were used. G1 (44) and G2 (46) received two applications of PGF2 (D0 and D11) and G3 (26) and G4 (37) received one application after 5 days of estrous detection. G1 and G3 also received a 1 mg ODB application 48 hours after the PGF2. The pregnancy rate at AI was 42,2% for G1, 47,8% for G2, 32,1% for G3 e 57,6% for G4 (p>0,05) and of 38,4% and 51,4% for animals submitted to FTAI and estrous detection, respectively (p>0,05). This results show that pregnancy rates of females fixed time inseminated didnt differ of that inseminated after estrus detection. Key-words: Beef cattle, estrus synchronization, fixed time artificial insemination, prostaglandin.
O objetivo deste estudo foi avaliar o emprego de um análogo da prostaglandina F2 (PGF2) em associação ao benzoato de estradiol (BE) em programas de sincronização de cios. Utilizaram-se 185 fêmeas não-lactantes divididas em quatro grupos. O G1 (44) e o G2 (46) receberam duas aplicações de PGF2 (D0 e D11) e os animais do G3 (26) e G4 (37) uma aplicação de PGF2, após cinco dias de detecção de cio. O G1 e o G3 receberam 1mg de BE 48 horas após a PGF2. A taxa de prenhez na IA foi de 42,2% no G1, 47,8% no G2, 32,1% no G3 e 57,6% no G4 (p>0,05) e de 38,4% e 51,4% para animais submetidos à IATF e à detecção de cio, respectivamente (p>0,05). Notou-se que a taxa de prenhez nas fêmeas inseminadas em tempo-fixo não diferiu daquelas que foram inseminadas após a detecção de cio. Palavras-chaves: Bovinos de corte, inseminação artificial em tempo fixo, prostaglandina, sincronização de cios.