RESUMO
Plinia cauliflora (Mart.) Kausel, popularly known as jabuticaba, possesses bioactive compounds such as flavonoids, tannins, and phenolic acids, known for their antioxidant, antibacterial, wound healing, and cardioprotective effects. Therefore, this study aimed to standardize the P. cauliflora fruit peel extraction method, maximize phenolic constituents, and evaluate their antioxidative and antimicrobial effects. Various extraction methods, including vortex extraction with and without precipitation at 25, 40, and 80 °C, and infusion extraction with and without precipitation, were performed using a completely randomized design. Extraction without precipitation (E - P) showed the highest yield (57.9%). However, the precipitated extraction (E + P) method displayed a yield of 45.9%, higher levels of phenolic derivatives, and enhanced antioxidant capacity. Major compounds, such as D-psicose, D-glucose, and citric acid, were identified through gas chromatography-mass spectrometry (GC-MS) analysis. Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis identified citric acid, hexose, flavonoids, tannins, and quercetin as the major compounds in the extracts. Furthermore, the extracts exhibited inhibitory effects against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli bacteria. In conclusion, the E + P method efficiently obtained extracts with high content of bioactive compounds showing antioxidant and antimicrobial capacities with potential application as a dietary supplement.
RESUMO
The objective of this work was to evaluate the photocatalytic activity of zinc oxide catalysts supported on natural zeolite clinoptilolite for photocatalytic degradation of the drug hydroxychloroquine, used in the treatment of malaria and which has been tested in the treatment of COVID-19. To synthesize 10%ZnOCP and 15%ZnOCP catalysts, the wet impregnation methodology was used. The raw and synthesized catalysts were characterized by XRD, SEM, XRF, BET, DRS, PCZ, FT-IR and PL. The degradation of hydroxychloroquine was calculated using UV-vis absorption from the samples before and after the photocatalytic process. The maximum percentage of degradation (96%) was obtained with the operational parameters of C0 = 10 mg L-1; Ccat = 2 g L-1 of 15%ZnOCP; pH = 7.5; UV-A radiation. Ecotoxicological tests against the bioindicators Lactuca sativa and Artemia salina confirmed the reduction of effluent toxicity after treatment.
Assuntos
Tratamento Farmacológico da COVID-19 , Zeolitas , Óxido de Zinco , Catálise , Humanos , Hidroxicloroquina , SARS-CoV-2 , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
On March 11, 2020, the World Health Organization (WHO) declared COVID-19 a pandemic. The outbreak caused a worldwide impact, becoming a health threat to the general population and its professionals. To date, there are no specific antiviral treatments or vaccines for the COVID-19 infection, however, some drugs are being clinically tested. The use of these drugs on large scale raises great concern about their imminent environmental risk, since the elimination of these compounds by feces and urine associated with the inefficiency of sewage treatment plants in their removal can result in their persistence in the environment, putting in risk the health of humans and of other species. Thus, the goal of this work was to conduct a review of other studies that evaluated the presence of the drugs chloroquine, hydroxychloroquine, azithromycin, ivermectin, dexamethasone, remdesivir, favipiravir and some HIV antivirals in the environment. The research indicated the presence of these drugs in the environment in different regions, with concentration data that could serve as a basis for further comparative studies following the pandemic.