RESUMO

This study aimed to develop protocols for the extraction of sperm proteins from Moxotó goats (Capra hircus) and to compare the resulting proteomic maps. The sperm proteins were isolated using an extraction buffer containing 7 M urea and 2 M thiourea, 20 mM DTT, and one of the following detergents: 1% or 4% CHAPS; 1% or 4% SDS; 1% or 4% Triton X-100; or a combination of CHAPS and SDS. The 1-DE and 2-DE profiles of the isolated proteins revealed that the various isolation methods were efficient. Qualitative and quantitative differences in the 1-DE and 2-DE profiles were observed. 2-DE maps indicated that the amount and diversity of proteins visualized depended on the detergent that was used. Furthermore, this work revealed that the combination of detergents increased the resolution of some spots and retained the characteristics of the individual detergents, depending on their concentrations.(AU)

Assuntos

RESUMO

This study aimed to develop protocols for the extraction of sperm proteins from Moxotó goats (Capra hircus) and to compare the resulting proteomic maps. The sperm proteins were isolated using an extraction buffer containing 7 M urea and 2 M thiourea, 20 mM DTT, and one of the following detergents: 1% or 4% CHAPS; 1% or 4% SDS; 1% or 4% Triton X-100; or a combination of CHAPS and SDS. The 1-DE and 2-DE profiles of the isolated proteins revealed that the various isolation methods were efficient. Qualitative and quantitative differences in the 1-DE and 2-DE profiles were observed. 2-DE maps indicated that the amount and diversity of proteins visualized depended on the detergent that was used. Furthermore, this work revealed that the combination of detergents increased the resolution of some spots and retained the characteristics of the individual detergents, depending on their concentrations.

Assuntos

RESUMO

Este artigo busca avaliar a eficiência técnica dos gastos municipais per capita em educação, saúde e assistência social para os municípios cearenses, referente a 2005. Para isso, utiliza-se da metodologia de análise envoltória de dados (DEA), orientada pelos insumos e retornos constantes de escala, uma vez que se pretende obter resultados voltados para diminuir o gasto público e manter o nível de eficiência na prestação dos serviços à comunidade cearense. Nestes termos, pode-se dizer que este exercício empírico procura estimar a eficiência técnica relativa, a eficiência escalar, bem como classificar os municípios analisados segundo essas medidas e, ainda, apontar os municípios que são considerados como benchmark para cada modelo. Os resultados mostram que o modelo gasto público, que agrega insumos e produtos dos serviços de educação, saúde e segurança, apresenta 55% dos municípios sobre a fronteira de eficiência relativa. Por outro lado, os modelos específicos de saúde, educação e segurança apontam baixa eficiência técnica no gasto público social. O estudo conclui que há certa ineficiência técnica no tocante aos gastos públicos municipais do Ceará com saúde, educação e segurança.

This research seeks to evaluate the technical efficiency of municipal per capita spending on education, health and social care for municipalities cearenses, covering the year 2005. For this, it uses the methodology of Data Envelopment Analysis (DEA), oriented input and constant returns to scale, because they want to achieve results aimed to reduce public spending and keep the level of efficiency in service delivery to Ceará community. Accordingly, we can say that this empirical exercise to estimate the demand on technical efficiency, the efficiency scale as well as rank the cities analyzed according to these measures and, moreover, indicate that the municipalities are considered as benchmark in each model. The results shows that the model that adds inputs and products of education services, health, safety exhibit 55% of the counties on the border of relative efficiency. Furthermore, the specific models of Health, Education and Safety show a low technical efficiency in public social spending. The study concludes that there are some technical inefficiency regarding public spending municipal Ceará with health, education and security

Esta investigación trata de evaluar la eficiencia técnica del gasto municipal per cápita en educación, salud y asistencia social a los municipios de Ceará para el año 2005. Para ello, se utiliza la metodología del Análisis Envolvente de Datos (DEA), guiados por los insumos y rendimientos constantes a escala, ya que se trat de resultados orientados a reducir el gasto público y mantener el nivel de eficiencia en la prestación de servicios a Comunidad de Ceará. En consecuencia se puede decir que este ejercicio empírico trata de estimar la eficiencia técnica relativa, la eficiencia de escala, y para clasificar a los municipios analizados de acuerdo con estas medidas y considerar los condados que son considerados como un punto de referencia para cada modelo. Los resultados muestran que el modelo de gasto público que agrega los insumos e productos de los servicios de educación, salud y seguridad, tiene 55% de los condados en la frontera de eficiencia relativa. Por otra parte, los modelos específicos de Salud, Educación y Seguridad muestran una baja eficiencia técnica en el gasto público social. El estudio concluye que hay una cierta ineficiencia técnica en cuanto a gasto público de Ceará con la salud municipal de educación y seguridad.

Assuntos