RESUMO
The objective of this work was to evaluate whether tetracycline (TC) in environmentally relevant concentrations was able to induce alterations to embryonic development and teratogenic effects in oocytes and embryos of Cyprinus carpio. For this purpose, an embryolethality study was conducted and the lethal concentration 50 (LC50) and effective concentration 50 of malformations (EC50) were calculated, and with these data the teratogenic index (TI) was determined. The main alterations to embryonic development and the teratogenic effects produced by TC on embryos of C. carpio were determined using the Kimmel and Hersem scale adapted for Cyprinus carpio. LC50 and EC50 were respectively 500.08 and 145.3⯵gâ¯L-1.TC was shown to be teratogenic with teratogenic index of 3.44, and the main malformations identified in concentrations of 90-900⯵gâ¯L-1 were malformation in tail, modified chorda structure, pericardical edema, scoliosis and malformations of the heart. A significant decrease in concentration-dependent in Kimmel and Hersem score was observed. The results allow us to conclude that TC at environmentally relevant concentrations is capable of inducing embryotoxic and teratogenic effects, generating risk in the integrity of the common carp C. Carpio.
Assuntos
Carpas/embriologia , Desenvolvimento Embrionário/efeitos dos fármacos , Poluentes Ambientais/toxicidade , Teratogênicos/farmacologia , Tetraciclina/toxicidade , Animais , Dose Letal Mediana , TeratogêneseRESUMO
Amoxicillin (AMX) is a pharmaceutical widely employed in human and veterinary medicine worldwide. Its wide production and use has led to this pharmaceutical being released into the environment in concentrations that range from ng L-1 to µg L-1. Previous studies have demonstrated that this antibiotic generates toxic effects, amongst which alterations to embryonic development and oxidative stress in aquatic organisms, is noteworthy. Nonetheless, it is necessary to characterize the risks that this pharmaceutical represents for species of economic interest such as Cyprinus carpio, in a more precise manner. The aim of this work was to demonstrate if AMX, at environmentally-relevant concentrations, is capable of inducing genotoxic/cytotoxic alterations in C. carpio. In order to evaluate genotoxicity, the comet assay and micronucleus test were used; in order to determine cytotoxic effects, caspase-3 activity and the TUNEL assay were carried out. The results showed that the effects of the biomarkers had their maximum at 72â¯h; considering the DNA damage in the comet assay, 0.039⯵gâ¯L-1 resulted in a 29% increase compared to control, and 1.67⯵gâ¯L-1 caused a 40% increase; micronucleus frequency increased by 205% in C1 and by 311% in C2 when compared to control; compared to control, caspase-3 activity increased 262% in C1 and 787% in C2; for the TUNEL assay, DNA fragmentation increased by 86% in C1 and 120% in C2 compared to control. The results showed that environmentally-relevant concentrations, AMX was capable of generating DNA damage and cytotoxic effects in blood cells of the common carp.
Assuntos
Amoxicilina/uso terapêutico , Antibacterianos/uso terapêutico , Poluição Ambiental/análise , Poluentes Químicos da Água/química , Amoxicilina/farmacologia , Animais , Antibacterianos/farmacologia , CarpasRESUMO
Sucralose (SUC) is an organochlorine that is used as a common sweetener in different dietary products around the world. Its extended use and production have led to this product is released into the environment in concentrations ranging from ngâ¯L-1 to µgâ¯L-1 in surface waters, groundwaters, wastewater treatment plants and ocean waters. A previous study carried out by our research team demonstrated that SUC is capable of inducing oxidative stress in Cyprinus carpio at environmentally-relevant concentrations. The aim of this study was to evaluate if SUC was capable of inducing alterations to DNA, apoptosis, and oxidative damage in the blood cells of C. carpio. Carps were exposed to two environmentally-relevant concentrations (0.05 and 155⯵gâ¯L-1) of SUC, and the following biomarkers were determined: comet assay, micronucleus test (MN), caspase-3 activity, TUNEL assay, hydroperoxide content, lipid peroxidation level, protein carbonyl content and superoxide dismutase and catalase activities. Results obtained showed that SUC is capable of inducing DNA damage. A maximum increase of 35% and 23% were observed for c1 and c2, respectively in the comet assay; increases of 586% and 507.7% for c1 and c2, respectively, were found at 72â¯h through the MN test. The activity of caspase-3 showed a greater response for c1 and c2 at 96â¯h, with 271% and 493.5%, respectively. TUNEL assay also showed the highest response at 96â¯h, with 51.8 for c1 and 72.9 for c2; c1 y c2 were able to induce oxidative stress with the highest expression at 72â¯h. A correlation between DNA damage biomarkers, apoptosis and plasma levels of SUC in both concentrations were observed. With the data obtained, we can conclude that SUC, at environmentally-relevant concentrations, was capable of generating DNA alterations, apoptosis and oxidative stress in blood cells in common carp.