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INTRODUCTION: Insomnia and vasomotor symptoms are frequently experienced during the menopausal transition and in postmenopause, worsening sleep maintenance and quality of life. Our study evaluated the use of lavender essential oil and sleep hygiene guidance on quality of life, sleep patterns and self-reported hot flashes in postmenopausal women with insomnia diagnosis. METHODS: This study is part of a larger experimental, double-blind, randomized controlled trial. We included 35 postmenopausal women with a diagnosis of insomnia who were distributed in two groups: A Placebo group (sunflower oil) and an Aroma group (Lavandula angustifolia essential oil); both inhaled the oils before bedtime for 29 days. The groups received sleep hygiene guidelines and weekly follow-up. Validated questionnaires were used to assess the effect of the intervention, and a sleep diary was used to assess sleep onset latency (SOL), total sleep time (TST), and sleep efficiency (SE) daily. Participants who complained of vasomotor symptoms at baseline kept a daily record of their frequency and intensity. RESULTS: All participants benefited in almost all domains of quality of life and in the self-assessed TST and SE. The Aroma group achieved a significant improvement in their overall quality of life score over time. No differences were observed in respect of the hot flashes record and daytime sleepiness. CONCLUSIONS: The aromatherapy intervention was effective in improving the overall quality of life in the Aroma group. Moreover, the sleep diary and sleep hygiene instructions helped all participants to, respectively, self-assess and improve their sleep patterns.

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The objective was to determine the zootechnical performance of broiler chickens fed different diets containing cassava meal. A total of 450 male broiler chickens of the Cobb lineage was used. The experimental design was completely randomized with five treatments (0, 25, 50, 75, and 100% inclusion of cassava meal) and five replications, composed of 18 birds per experimental unit. Morphometric characteristics of broiler chickens were evaluated: live weight, and weights of full and empty carcasses, livers, hearts, full and empty gizzards, abdominal fat, wings, thighs, drumsticks, breasts, and dorse. Data were tested using an analysis of variance, regression model, and cluster and discriminant analyses. There was a difference in the weight of the heart, full gizzard, wing, thigh, drumstick, and breast in relation to the different diets. The inclusion of 8.2%, 57.57%, and 25.38% cassava meal maximized thighs at 323.96 g, drumsticks at 385.04 g, and breasts at 921.12 g, respectively. The formation of two groups of birds was verified, and the classification rate was 92%. Inclusion of up to 50% cassava meal in the broiler diet did not alter its zootechnical performance, implying a lower cost of production.

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BACKGROUND: Women with pain present decreased sleep duration and quality of life, but little is known about the consequences of nighttime pain on overall health. Our goal was to compare levels of anxiety, depression and fatigue in women with pain during the night with those without pain. We hypothesized that pain perception is associated with the worsening of fatigue and mood. METHODS: In total, 244 women aged 20-80 years who took part in the Epidemiologic Sleep Study (EPISONO) met the inclusion criteria, 85 in the self-reported pain group and 171 in the control group. Participants were assigned to the pain group if they both responded (i) they generally had pain, according to Pre-Sleep Questionnaire (PSQ) and (ii) their pain generally interfered with their sleep 3 or more times a week, according to Pittsburg Sleep Quality Index (PSQI). Those who answered negatively to both questions were allocated to the control group. All participants underwent a full night laboratory polysomnography, and completed questionnaires related to fatigue, depression, anxiety and quality of life. RESULTS: Pain group participants had a significantly lower perception of quality of life, and significantly higher levels of fatigue (5.4 times), anxiety and depression. Pain perception was also correlated to levels of anxiety and depression in the psychological (rs  = -0.463, -0.607, respectively) and social (rs  = -0.423, -0.438, respectively) quality of life domains. CONCLUSIONS: Nighttime pain in women was associated with decreased quality of life, worsening mood and fatigue. Our data shows the importance of investigating pain and its deleterious effects on women's health. SIGNIFICANCE: Our study highlights that women experiencing nighttime pain also reported worse mood outcomes and decreased quality of life, regardless of the level and type of pain. Our data, based on association analysis and not investigating causality, suggest it is important to consider nighttime pain in clinical care to improve quality of life and general health.

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Invasive insects cost the global economy around USD 70 billion per year. Moreover, increasing agricultural insect pests raise concerns about global food security constraining and infestation rising after climate changes. Current agricultural pest management largely relies on plant breeding-with or without transgenes-and chemical pesticides. Both approaches face serious technological obsolescence in the field due to plant resistance breakdown or development of insecticide resistance. The need for new modes of action (MoA) for managing crop health is growing each year, driven by market demands to reduce economic losses and by consumer demand for phytosanitary measures. The disabling of pest genes through sequence-specific expression silencing is a promising tool in the development of environmentally-friendly and safe biopesticides. The specificity conferred by long dsRNA-base solutions helps minimize effects on off-target genes in the insect pest genome and the target gene in non-target organisms (NTOs). In this review, we summarize the status of gene silencing by RNA interference (RNAi) for agricultural control. More specifically, we focus on the engineering, development and application of gene silencing to control Lepidoptera through non-transforming dsRNA technologies. Despite some delivery and stability drawbacks of topical applications, we reviewed works showing convincing proof-of-concept results that point to innovative solutions. Considerations about the regulation of the ongoing research on dsRNA-based pesticides to produce commercialized products for exogenous application are discussed. Academic and industry initiatives have revealed a worthy effort to control Lepidoptera pests with this new mode of action, which provides more sustainable and reliable technologies for field management. New data on the genomics of this taxon may contribute to a future customized target gene portfolio. As a case study, we illustrate how dsRNA and associated methodologies could be applied to control an important lepidopteran coffee pest.

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Two Bemisia tabaci (Gennadius) species, Middle East-Asia Minor 1 (MEAM1) and Mediterranean (MED), are major pests that are dispersed throughout the world. While MEAM1 was introduced in Brazil in the 1990s, MED was reported recently with limited spread. Here, a survey was performed to examine whether MED whiteflies are widely present in the Federal District region, in central Brazil. Whiteflies were collected in various locations in the Federal District and surroundings between 2018 and 2020, including garden centers and small- and large-scale farms. The species were identified using RFLPand sequencing of the mitochondrial cytochrome c oxidase I subunit gene region. Out of 108 whitefly batches, 63.89% were composed exclusively by MEAM1, followed by 16.67% presenting only MED, and another 7.40% containing unidentified whitefly species (NI). Plant varieties serving as hosts for more than one whitefly species were observed in 12.04% of the samples, either by MEAM1/MED, MEAM1/NI, or MED/NI. This study highlights the still limited presence of MED in the Federal District and surroundings, predominantly in garden centers and in the green belt of Brasília, closer to urban areas. In contrast, only MEAM1 was identified in large-scale cultivated areas.

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BACKGROUND: To evaluate the effect of Lavandula angustifolia essential oil inhalation on sleep and menopausal symptoms in postmenopausal women with insomnia. PARTICIPANTS: 35 postmenopausal women with a clinical diagnosis of insomnia were included, 17 in Aroma Group (AG) and 18 in Placebo Group (PG). METHODS: In this double-blind, randomized controlled trial, PG participants inhaled sunflower oil and AG participants inhaledLavandula angustifolia essential oil, for 29 days. Both groups received sleep hygiene guidelines before the intervention and weekly follow-up during it. Evaluations were performed before and after intervention. All statistical analyses and intention-to-treat test were performed in SPSS 22. Sleep quality (Primary outcome) was measured by Pittsburgh Sleep Quality Index. Secondary outcomes were polysomnography data, severity of insomnia, anxiety and depression symptoms, and postmenopausal symptoms. RESULTS: There were no significant differences between groups after intervention in the primary outcome (P = 0.22; effect size=0.69); however, a tendency of improvement in wake after sleep onset (WASO) was observed (P = 0.07; effect size=0.81; B = 42.2). Both groups presented better sleep quality over time (AG P < 0.001; PG P = 0.011). AG participants showed a significant decrease in sleep onset latency (P = 0.001), depression levels (P = 0.025), hot flashes (P < 0.001), postmenopausal symptoms (P < 0.001) and, in polysomnography data, increased sleep efficiency (P = 0.002) compared to baseline. CONCLUSION: Although no significant differences were observed between groups, our data presented a tendency of improvement in WASO. Moreover, AG participants had enhanced overall sleep pattern, quality and sleep efficiency. Weekly follow-up and sleep hygiene instructions were essential for both groups to show improvement in almost all outcomes. CLINICAL TRIAL REGISTRATION: Brazilian Registry of Clinical Trials, www.ensaiosclinicos.gov.br, RBR-5q5t5z.

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Agricultural production in semi-arid regions is limited due to water availability. In addition, the water quality available for irrigation is often compromised due to the high salt content present. Millet is a forage species considered tolerant to water deficit and moderately salt tolerant. In view of the above, the objective was to evaluate the growth of millet under water and saline stress associates. The experiment was carried out in a randomized block design, in a 4x3 factorial scheme, composed of four levels of water replacement, based on crop evapotranspiration (ETc): 25%.ETc, 50%.ETc, 75%.ETc and 100%.ETc and three levels of water salinity (0.03, 2.0 and 4.0 dS m-1). With 25%.ETc independent of salinity, all morphological characteristics of millet were affected, occurring death of plants in the initial growth phase. In the absence of salt and greater availability of water, greater plant growth occurred. With respect to salinity, there was a reduction in the increment of all variables evaluated, with the highest reduction at the highest saline level (4.0 dS m-1). Water and salt stresses, when associated, reduce the growth of millet, since concentrations above 2.0 dS m-1 and less than 50%.ETc compromise its full development, providing declines in yield.(AU)

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Agricultural production in semi-arid regions is limited due to water availability. In addition, the water quality available for irrigation is often compromised due to the high salt content present. Millet is a forage species considered tolerant to water deficit and moderately salt tolerant. In view of the above, the objective was to evaluate the growth of millet under water and saline stress associates. The experiment was carried out in a randomized block design, in a 4x3 factorial scheme, composed of four levels of water replacement, based on crop evapotranspiration (ETc): 25%.ETc, 50%.ETc, 75%.ETc and 100%.ETc and three levels of water salinity (0.03, 2.0 and 4.0 dS m-1). With 25%.ETc independent of salinity, all morphological characteristics of millet were affected, occurring death of plants in the initial growth phase. In the absence of salt and greater availability of water, greater plant growth occurred. With respect to salinity, there was a reduction in the increment of all variables evaluated, with the highest reduction at the highest saline level (4.0 dS m-1). Water and salt stresses, when associated, reduce the growth of millet, since concentrations above 2.0 dS m-1 and less than 50%.ETc compromise its full development, providing declines in yield.

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Pregnancy is a period of major transformations in a woman's life; increased stress, and mood and sleep disorders are frequent. This review evaluates mindfulness interventions during pregnancy and their ability to help manage stress, anxiety, depression, emotional regulation, level of mindfulness and sleep quality. A search of English language scientific literature relevant to mindfulness interventions for pregnant women was conducted using PubMed, Scopus and Web of Science, without restriction on publication date. Inclusion criteria were randomized clinical trials with pregnant women, using mindfulness as an intervention for at least three weeks, in one of our main areas of interest, and using only validated scales to measure outcomes. Two hundred and thirty studies were identified in our searches of research databases, and thirteen were included in our analysis. We found a large diversity of mindfulness programs, heterogeneity among the instruments used to evaluate outcomes, and inconsistency in the gestational periods used in the studies. Mindfulness interventions were beneficial for stress, anxiety and depression. Mindfulness was also effective when applied in pregnant women with a history of depression or experiencing depression. Considering emotional regulation and the level of mindfulness, there were signs of improvement, but more studies are needed. None of the studies evaluated sleep quality. Our review provides information about current mindfulness programs, an overview of the effects of mindfulness interventions, a description of the measurements used so far, and recommendations for developing high-quality mindfulness protocols for pregnant women.

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OBJECTIVES: Sleep is essential for health and well-being and lack of sleep can have serious physiological consequences. This study aimed to evaluate sleep patterns and the influence of insomnia on quality of life. DESIGN: The epidemiologic sleep study is a population-based study of sleep and risk factors for sleep disturbances. SETTING AND PARTICIPANTS: This cross-sectional study recruited 574 men and 468 women aged 18 years or older randomly, to represent the population of Sao Paulo, according to gender, age, and socioeconomic status. MEASUREMENTS: Data from polysomnography were used to assess sleep objectively and a validate questionnaire to assess quality of life. Validated questionnaires based on the Diagnostic and Statistical Manual of Mental Disorders, fourth edition, assessed the perception of insomnia and categorized the sample as: without insomnia symptoms, insomnia symptoms, and insomnia syndrome. Anthropometric data, objective sleep parameters and quality of life were assessed and the sample was distributed according to age for both genders. RESULTS: Participants in both insomnia groups presented a worse perception of quality of life compared to without insomnia symptoms group in both genders. Women had a lower percentage of participants without insomnia symptoms (33.3%), and a higher percentage of insomnia symptoms (48.6%), and insomnia syndrome (18.1%) than men (42.1%, 47.2%, and 10.7%, respectively). CONCLUSIONS: Women presented more insomnia complaints and had a lower perceived quality of life compared to men, especially at young ages. Men and women with insomnia symptoms or insomnia syndrome had a lower quality of life score.

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Pasture studies require information on leaf area, as it is one of the main parameters for evaluation of plant growth. Thus, the objective of this study was to estimate the leaf blade area of pangolão grass (Digitaria pentzii Stent.) using non-destructive methods by regression model analysis. The experimental design consisted of randomized blocks, with three cutting heights (10, 15, and 20 cm) and four replications. Three hundred leaf blades of pangolão grass were randomly collected, and their respective lengths (L) and widths (W) determined using a digital caliper. The leaf blade area of pangolão grass was estimated by the gravimetric method, using linear and power regression models to explain the leaf blade area as a function of the product of L and maximum W. The real leaf blade area presented an average value of 18.64 cm2, ranging from 4.29 to 45.95 cm2. The leaf blade area of pangolão grass, regardless of cutting height, was estimated with greater accuracy by the power model. The power model, Ŷ=LW1.007, can be used to estimate the leaf blade area of pangolão grass based on leaf blade L and W values.(AU)

Estudos com pastagens necessitam de informações sobre a área foliar, por ser um dos principais parâmetros de avaliação do crescimento das plantas. Desse modo, objetivou-se estimar a área da lâmina foliar do capim-pangolão (Digitaria pentzii Stent.), utilizando métodos não destrutivos por meio de análise de modelos de regressão. O delineamento utilizado foi em blocos casualizados, com três alturas de corte (10, 15 e 20 cm) e quatro repetições. Foram coletadas aleatoriamente 300 lâminas foliares do capim-pangolão e determinados os seus respectivos comprimentos (C) e larguras (L), com uso de paquímetro digital. A área da lâmina foliar do capim-pangolão foi estimada pelo método gravimétrico, sendo utilizados os modelos de regressão linear e potência para explicar a área das lâminas foliares em função do produto do comprimento e máxima largura. A área da lâmina foliar real apresentou valor médio de 18,64 cm2, variando de 4,29 a 45,95 cm2. A área da lâmina foliar do capim-pangolão, independentemente da altura de corte, foi estimada com melhor acurácia pelo modelo potência. O modelo potência, Ŷ=CL1,007, pode ser usado para estimar a área da lâmina foliar do capim-pangolão com base nos valores de comprimento e largura da lâmina foliar dessa espécie.(AU)

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Pasture studies require information on leaf area, as it is one of the main parameters for evaluation of plant growth. Thus, the objective of this study was to estimate the leaf blade area of pangolão grass (Digitaria pentzii Stent.) using non-destructive methods by regression model analysis. The experimental design consisted of randomized blocks, with three cutting heights (10, 15, and 20 cm) and four replications. Three hundred leaf blades of pangolão grass were randomly collected, and their respective lengths (L) and widths (W) determined using a digital caliper. The leaf blade area of pangolão grass was estimated by the gravimetric method, using linear and power regression models to explain the leaf blade area as a function of the product of L and maximum W. The real leaf blade area presented an average value of 18.64 cm2, ranging from 4.29 to 45.95 cm2. The leaf blade area of pangolão grass, regardless of cutting height, was estimated with greater accuracy by the power model. The power model, Ŷ=LW1.007, can be used to estimate the leaf blade area of pangolão grass based on leaf blade L and W values.

Estudos com pastagens necessitam de informações sobre a área foliar, por ser um dos principais parâmetros de avaliação do crescimento das plantas. Desse modo, objetivou-se estimar a área da lâmina foliar do capim-pangolão (Digitaria pentzii Stent.), utilizando métodos não destrutivos por meio de análise de modelos de regressão. O delineamento utilizado foi em blocos casualizados, com três alturas de corte (10, 15 e 20 cm) e quatro repetições. Foram coletadas aleatoriamente 300 lâminas foliares do capim-pangolão e determinados os seus respectivos comprimentos (C) e larguras (L), com uso de paquímetro digital. A área da lâmina foliar do capim-pangolão foi estimada pelo método gravimétrico, sendo utilizados os modelos de regressão linear e potência para explicar a área das lâminas foliares em função do produto do comprimento e máxima largura. A área da lâmina foliar real apresentou valor médio de 18,64 cm2, variando de 4,29 a 45,95 cm2. A área da lâmina foliar do capim-pangolão, independentemente da altura de corte, foi estimada com melhor acurácia pelo modelo potência. O modelo potência, Ŷ=CL1,007, pode ser usado para estimar a área da lâmina foliar do capim-pangolão com base nos valores de comprimento e largura da lâmina foliar dessa espécie.

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The experiment was carried out to evaluate the productive performance of European quails (Coturnix coturnix) in the production phase, fed with diets containing different levels of digestible lysine. A total of 175 female quails, aged 65 days, were randomly distributed in a completely randomized design with five treatments and five replicates per treatment. The animals were housed in 25 metal cages, and each cage represented an experimental parcel, with seven birds per parcel. The birds were fed the experimental rations containing 1.177, 1.217, 1.317, 1.417, and 1.517% digestible lysine. The parameters evaluated were: laying rate, mass of eggs produced, feed intake in the period, lysine intake, feed conversion per dozen and per mass. Significant differences were observed for posture rate with quadratic behavior, which indicated higher production for the level of 1.23% of digestible lysine. There was an increasing linear behavior for the feed and lysine intake, and for conversion feed per dozen and by mass, we observed quadratic behaviors. This indicates better feed conversion index when the optimal inclusion level was 1.33% and 1.404% of digestible lysine in the diet, respectively. The requirement of digestible lysine in diets for European quails is 1.404%, which corresponds to a daily intake of 421.20 milligrams of digestible lysine.(AU)

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The experiment was carried out to evaluate the productive performance of European quails (Coturnix coturnix) in the production phase, fed with diets containing different levels of digestible lysine. A total of 175 female quails, aged 65 days, were randomly distributed in a completely randomized design with five treatments and five replicates per treatment. The animals were housed in 25 metal cages, and each cage represented an experimental parcel, with seven birds per parcel. The birds were fed the experimental rations containing 1.177, 1.217, 1.317, 1.417, and 1.517% digestible lysine. The parameters evaluated were: laying rate, mass of eggs produced, feed intake in the period, lysine intake, feed conversion per dozen and per mass. Significant differences were observed for posture rate with quadratic behavior, which indicated higher production for the level of 1.23% of digestible lysine. There was an increasing linear behavior for the feed and lysine intake, and for conversion feed per dozen and by mass, we observed quadratic behaviors. This indicates better feed conversion index when the optimal inclusion level was 1.33% and 1.404% of digestible lysine in the diet, respectively. The requirement of digestible lysine in diets for European quails is 1.404%, which corresponds to a daily intake of 421.20 milligrams of digestible lysine.

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This study adjusted different regression models to describe the growth pattern of meat quails from birth to 42 days of age. Data of 300 male quails were used. Weight and height information of all quails were collected weekly from the 1st to the 42nd day of age. Body weight of poultry was subjected to the polynomial, logistic, Gompertz, Weibull, and log-normal regression models. The criteria used to choose the best model to explain the growth curve of quails were the coefficient of determination of the model, Akaikes information criterion, sum of squared residuals and Willmotts index. For all the models used, the variables age and height were significant to explain the weight of quails. The polynomial (R² = 99.99%, AIC = 24.68, SSR = 27.5, d = 0.9999) and log-normal (R² = 99.60%, AIC = -17.5, SSR = 107.15, d = 0.9989) models presented the best fit criteria and were recommended to explain the growth of quails.(AU)

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Leaf area measurements are of the main parameters used in agronomic studies to evaluate plant growth. The current study used a non-destructive method based on linear leaf dimensions (length and width) to select the regression model to estimate millet (Pennisetum glaucum) leaf area. For two millet genotype (IPA BULK 1 BF and ADR 300) 128 randomly-chosen leaves were measured at different vegetative growth stages. Measures of length and width of each leaf were made using digital calipers. Leaf area was measured using a gravimetric method. The best-fit leaf area estimation model was selected via linear, potential and gamma regression models. Leaf area values varied from 3.02 to 209.21 cm2 . The average value was 95.31 cm2 . The potential regression model exhibited lower residual sum of squares and Akaike's information criterion and similar determination coefficient and Willmott index. Thus, potential regression was more efficient in explaining the leaf area of millet, independent of the genotype, when compared to other models evaluated in this research. Length (L) and width (W) could be used in the following potential regression model to estimate millet leaf blade.(AU)

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This study adjusted different regression models to describe the growth pattern of meat quails from birth to 42 days of age. Data of 300 male quails were used. Weight and height information of all quails were collected weekly from the 1st to the 42nd day of age. Body weight of poultry was subjected to the polynomial, logistic, Gompertz, Weibull, and log-normal regression models. The criteria used to choose the best model to explain the growth curve of quails were the coefficient of determination of the model, Akaikes information criterion, sum of squared residuals and Willmotts index. For all the models used, the variables age and height were significant to explain the weight of quails. The polynomial (R² = 99.99%, AIC = 24.68, SSR = 27.5, d = 0.9999) and log-normal (R² = 99.60%, AIC = -17.5, SSR = 107.15, d = 0.9989) models presented the best fit criteria and were recommended to explain the growth of quails.

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Leaf area measurements are of the main parameters used in agronomic studies to evaluate plant growth. The current study used a non-destructive method based on linear leaf dimensions (length and width) to select the regression model to estimate millet (Pennisetum glaucum) leaf area. For two millet genotype (IPA BULK 1 BF and ADR 300) 128 randomly-chosen leaves were measured at different vegetative growth stages. Measures of length and width of each leaf were made using digital calipers. Leaf area was measured using a gravimetric method. The best-fit leaf area estimation model was selected via linear, potential and gamma regression models. Leaf area values varied from 3.02 to 209.21 cm2 . The average value was 95.31 cm2 . The potential regression model exhibited lower residual sum of squares and Akaike's information criterion and similar determination coefficient and Willmott index. Thus, potential regression was more efficient in explaining the leaf area of millet, independent of the genotype, when compared to other models evaluated in this research. Length (L) and width (W) could be used in the following potential regression model to estimate millet leaf blade.

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BACKGROUND: Obesity is characterized by excessive accumulation of body fat, which causes damage to the health of individuals, such as breathing difficulties. AIM: To verify the results of non-invasive ventilation as a preventive strategy on the decline of respiratory function and postoperative complications in patients undergoing Roux-en-Y gastric bypass. METHODS: This is a randomized trial, according to CONSORT standards, with obese adults aged 18-40 years. Randomized control group (n=25) only received guidelines regarding posture, early ambulation and cough stimuli, and in the NIV group (n=25), in addition to the aforementioned group, non-invasive ventilation was performed with two pressure levels, once day for 60 min, from the 1st to the 3rd postoperative day (POD). Both groups were evaluated in the preoperative period and in the 1st and 3rd POD for respiratory function, which were: slow vital capacity (VC), inspiratory capacity (IC), minute volume (MV), tidal volume maximal inspiratory muscle strength (Pimax) and peak expiratory flow (PEF). The length of hospital stay and the episodes of postoperative complications were recorded. RESULTS: Of the 50 patients the majority were young adults with degrees of obesity between III and IV. In the intergroup analysis, there was an improvement in the CVL and MV only in the 1st POD in the NIV group, CI in the three moments evaluated in the NIV group and the PFE in the 1st and 3rd PDO also in this group. The most frequent complications were pneumonia, followed by operative wound infection and atelectasis. There was a significant difference between groups, showing a higher occurrence in pneumonia and atelectasis in the control group. The days of hospitalization and intensive care unit were similar. CONCLUSION: It was observed a faster recovery until the 3rd POD in the IC and PEF variables in the NIV group; in addition, there were fewer complications in this group.

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