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Background: Rheumatoid arthritis (RA) in elderly population represents a challenge for physicians in terms of therapeutic management. Methotrexate (MTX) is the first-line treatment among conventional synthetic-disease-modifying anti-rheumatic drugs (cs-DMARDs); however, it is often associated with adverse events (AEs). Therefore, the objective of this study was to identify the incidence and risk factors of MTX discontinuation due to AEs in elderly patients with RA in a long-term retrospective cohort study. Methods: Clinical sheets from elderly RA patients taking MTX from an outpatient rheumatology consult in a university centre were reviewed. To assess MTX persistence, we used Kaplan-Meir curves and Cox regression models to identify the risk of withdrawing MTX due to adverse events. Results: In total, 198 elderly RA patients who reported using MTX were included. Of them, the rates of definitive suspension of MTX due to AEs were 23.0% at 5 years, 35.6% at 10 years and 51.7% at 15 years. The main organs and system involved were gastrointestinal (15.7%) and mucocutaneous (3.0%). Factors associated with withdrawing MTX due to AEs were MTX dose ≥ 15 mg/wk (adjusted HR: 2.46, 95% CI: 1.22-4.96, p = 0.012); instead, the folic acid supplementation was protective for withdrawal (adjusted HR: 0.28, 95% CI: 0.16-0.49, p < 0.001). Conclusions: Higher doses of MTX increase the risk of withdrawals in elderly RA, while folic acid supplementation reduces the risk. Therefore, physicians working in therapeutic management for elderly patients using MTX must focus on using lower MTX doses together with the concomitant prescription of folic acid.
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Systemic lupus erythematosus (SLE) is a multisystem disease considered a prototype of the main autoimmune disease and presents serious complications, such as lupus nephritis (LN), which generates a significant impact on morbidity and mortality. The SPP1 gene encodes the osteopontin (OPN) protein, which plays a crucial role in the regulation of inflammation and immunity. The variants rs1126616 and rs9138 of this gene have been associated with the inflammatory response. The study aims to analyze the association of the rs1126616 and rs9138 variants of the SPP1 gene in SLE Mexican-Mestizo patients without LN (SLE-LN). In this cross-sectional study, a total of 171 genomic DNA samples from SLE patients were clinically confirmed, of which 111 were SLE without LN, 60 were SLE with LN, and 100 healthy individuals were included as reference group. The rs1126616 variant was genotyped using PCR-RFLPs, and the rs9138 variant was genotyped using qPCR TaqMan. The TT genotype, the recessive model [OR 2.76 (95% CI 1.31-5.82), p = 0.011], and the T allele [OR 2.0 (95% CI 1.26-3.16), p = 0.003] of the rs1126616 variant are risk factors for SLE with LN. By contrast, the rs9138 variant did not show statistically significant differences among SLE patients stratified by LN. In our study of SLE Mexican-Mestizo patients with and without NL, demographic and clinical characteristics do not differ from other SLE populations, and the TT genotype of the rs1126616 variant of the SPP1 gene confers a risk factor for the presentation of LN. Otherwise, the rs9138 variant did not show association with NL.
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Lúpus Eritematoso Sistêmico , Nefrite Lúpica , Humanos , Nefrite Lúpica/genética , Estudos Transversais , Lúpus Eritematoso Sistêmico/genética , Alelos , Genótipo , OsteopontinaRESUMO
Introducción: El embarazo en la adolescencia es un problema de salud pública en Perú; sin embargo, hay una cifra de adolescentes con vida sexual activa, sin protección anticonceptiva. Objetivo: Determinar los factores socioculturales, sexuales y reproductivos asociados al no uso de métodos anticonceptivos en adolescentes mujeres. Métodos: Investigación descriptiva, transversal; se aplicó un análisis de base secundaria de la Encuesta Demográfica y Salud Familiar realizada en Perú en 2019. La población estuvo conformada por 1 871 mujeres de 15 a 19 años y se excluyeron aquellas que no habían iniciado su vida sexual o con datos incompletos. Se utilizó prueba ji cuadrado de Pearson y regresión de Poisson para el análisis estadístico. Resultados: El 46,6 por ciento de adolescentes no utilizaron métodos anticonceptivos. Se asociaron al no uso de métodos anticonceptivos, factores socioculturales como edad de 15 a 17 años, nivel educativo superior, asistencia actual a institución educativa, razón para dejar de estudiar (p< 0,05); y factores sexuales y reproductivos como: no tener parejas sexuales actualmente, edad de la primera relación sexual entre 15 a 19 años, no tener hijos y no vivir con una pareja (p< 0,05). Según el análisis multivariado, tener 15 a 17 años aumenta la prevalencia de no usar métodos anticonceptivos (Rpa: 1,21 IC: 1,09-1,33). Sin embargo, tener parejas sexuales (Rpa: 0,48 IC: 0,44-0,52) e hijos (Rpa: 0,49 IC: 0,45-0,55) redujeron la prevalencia de no uso de anticonceptivos. Conclusiones: Los factores socioculturales, sexuales y reproductivos asociados al no uso de anticoncepción fueron la edad de 15 a 17 años, tener parejas sexuales e hijos(AU)
Introduction: Teenage pregnancy is a public health problem in Peru; however, there is a number of sexually active adolescents without contraceptive protection. Objective: To determine the sociocultural, sexual and reproductive factors associated with the non-use of contraceptive methods in female adolescents. Methods: Descriptive, cross-sectional research; a secondary base analysis of the Demographic and Family Health Survey carried out in Peru, 2019 was applied. The population consisted of 1871 women between the ages of 15 to 19 years and those who had not started their sexual life or with incomplete data were excluded. Pearson's ji-square test and Poisson Regression were used for statistical analysis. Results: 46,6 percent of adolescents did not use contraceptive methods. Non-use of contraceptive methods was associated with sociocultural factors such as age between 15 to 17 years, higher educational level, current attendance at an educational institution, reason for leaving school (p< 0,05); and sexual and reproductive factors such as: not currently having sexual partners, age of first sexual intercourse between 15 and 19 years, not having children and not living with a partner (p< 0,05). According to the multivariate analysis, being 15 to 17 years old increases the prevalence of not using contraceptive methods (RPa: 1,21 CI: 1,09-1,33). However, having sexual partners (RPa: 0,48 CI: 0,44-0,52). However, having sexual partners (RPa: 0,48 CI: 0,44-0,52) and children (RPa: 0,49 CI: 0,45-0,55) reduced the prevalence of non-use of contraceptives. Conclusions: The sociocultural, sexual and reproductive factors associated with the non-use of contraception were the age of 15 to 17 years, having sexual partners and children(AU)
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Humanos , Gravidez , Adolescente , Fatores Sexuais , Fatores de Risco , Anticoncepção/métodos , Dispositivos Anticoncepcionais Femininos , Fatores Culturais , Planejamento Familiar , Fatores Sociais , Peru , Demografia/métodos , Epidemiologia Descritiva , Estudos Transversais , Determinantes Sociais da Saúde/tendênciasRESUMO
Aridoamerica and Mesoamerica are two distinct cultural areas in northern and central Mexico, respectively, that hosted numerous pre-Hispanic civilizations between 2500 BCE and 1521 CE. The division between these regions shifted southward because of severe droughts ~1100 years ago, which allegedly drove a population replacement in central Mexico by Aridoamerican peoples. In this study, we present shotgun genome-wide data from 12 individuals and 27 mitochondrial genomes from eight pre-Hispanic archaeological sites across Mexico, including two at the shifting border of Aridoamerica and Mesoamerica. We find population continuity that spans the climate change episode and a broad preservation of the genetic structure across present-day Mexico for the past 2300 years. Lastly, we identify a contribution to pre-Hispanic populations of northern and central Mexico from two ancient unsampled "ghost" populations.
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Estruturas Genéticas , Hispânico ou Latino , Humanos , História Antiga , México , Dinâmica PopulacionalRESUMO
Resumen La Alta Capacidad intelectual (ACI) es una manifestación diferencial de la inteligencia humana, de base neurobiológica, pero que debe expresar su alto potencial a lo largo del desarrollo de la per sona que la posee, mediante la covariación de factores moduladores endógenos (como la competencia social) y exógenos. El objetivo del trabajo es doble: 1) conocer, comparativamente la competencia social de menores con y sin ACI, 2) diferenciar aquellas competencias sociales que podrían ser factores protectores o de riesgo frente al mal uso de las tecnologías digitales. Se administra la Social Skills Improvement System-Rating Scales a una muestra de n = 70 aprendices (n = 35 con ACI, n = 35 con inteligencia promedio) de 11 a 16 años, analizando si existen diferencias estadísticamente significativas en habilidades sociales y en dificultades de conducta. Los resultados muestran diferencias estadísticamente significativas, a favor de los participantes con ACI en habilidades sociales (especialmente en: Responsabilidad, Cooperación y Autocontrol) y mejor ajuste personal, con baja inci dencia de dificultades internalizantes y externalizantes. Se concluye y discute el rol protector de las habilidades sociales para afrontar contextos interactivos complejos como el derivado de la era digital y agresiones como el cyberbullying.
Abstract High Intellectual Ability (HIA) is a differential manifestation of human intelligence with a neurobiologi cal basis but which must express its high potential along the developmental trajectory through the covariation of endogenous (such as social competence) and exogenous modulating factors. The aim of the study is twofold: 1) to know, comparatively, the social competence of children with and without HIA, 2) to differentiate those social competences that could be protective or risk factors against the misuse of digital technologies. The Social Skills Improvement System-Rating Scales were administered to a sample of n = 70 learners (n = 35 with ICA, n = 35 with average intelligence) aged 11 to 16, analysing whether there are statistically significant differences in social skills and behavioural difficulties. Results show statistically significant differences in favour of participants with ICA in social skills (especially in: Responsibility, Co-operation and Self-Control) and better personal adjustment, with low incidence of internalising and externalising difficulties. We conclude and discuss the protective role of social skills in coping with complex interactive contexts such as the digital age and aggressions such as cyberbullying.
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Objetivo: Determinar la prevalencia y factores asociados de la lactancia materna exclusiva en el Perú. Métodos: Estudio analítico transversal, realizado a partir de las bases de datos de la Encuesta Demográfica de Salud Familiar del Perú, 2020. Se incluyeron 41 258 mujeres de 15 a 49 años que tuvieron al menos un hijo en los últimos 5 años. Se utilizó la prueba Chi cuadrado de Pearson y la razón de prevalencia cruda (RPc) y ajustada (RPa) para el análisis bivariado; y la regresión de Poisson para el multivariado. Se utilizó el programa STATA versión 14. Resultados: La lactancia materna exclusiva tuvo una prevalencia de 70,43 %. Los factores sociodemográficos asociados fueron ser de la sierra [RPa: 1,39; IC95 %: 1,35-1,43] y de zona rural [RPa: 1,95; IC95 %: 1,93-1,96]. En cuanto a los factores obstétricos, la capacitación en lactancia materna exclusiva [RPa: 1,05; IC 95 %:1,03-1,06] incrementa la probabilidad de cumplimiento; mientras que, el sangrado excesivo en el parto [RPa: 0,97; IC95 %: 0,94-0,99] y la infección en los senos durante el posparto [RPa: 0,94; IC95 %: 0,91-0,97] la reducen. Entre los factores neonatales, tener un hijo macrosómico e iniciar precozmente la lactancia materna [RPa: 0,73; IC95 %: 0,68-0,80] están asociados a la lactancia materna exclusiva. Conclusiones: En el Perú, la lactancia maternal exclusiva tiene una elevada prevalencia, además su éxito está asociado de manera significativa con distintos factores sociodemográficos, obstétricos y neonatales(AU)
Objective: To determine the prevalence and associated factors of exclusive breastfeeding in Peru. Methods: Cross-sectional analytical study, based on the databases of the 2020 Peruvian Family Health Demographic Survey. We included 41 258 women aged 15-49 years who had at least one child in the last 5 years. Pearson's Chi-square test and the crude prevalence ratio (cPR) and adjusted prevalence ratio (aPR) were used for the bivariate analysis; and Poisson regression was used for the multivariate analysis. The STATA version 14 program was used. Results: Exclusive breastfeeding had a prevalence of 70.43%. The sociodemographic factors associated were being from the highlands [aPR: 1.39; 95% CI: 1.35-1.43] and rural area [aPR: 1,95; IC95 %: 1,93-1,96]. Regarding obstetric factors, exclusive breastfeeding training [aPR: 1.05; 95% CI: 1.03-1.06] increases the probability of compliance, while excessive bleeding in labor [aPR: 0.97; 95% CI: 0.94-0.99] and postpartum breast infection [aPR: 0.94; 95% CI: 0.91-0.97] reduce it. Among neonatal factors, having a macrosomic child and early initiation of breastfeeding [aPR: 0.73; 95%CI: 0.68-0.80] are associated with exclusive breastfeeding. Conclusions: In Peru, exclusive breastfeeding has a high prevalence, moreover, its success is significantly associated with different sociodemographic, obstetric and neonatal factors(AU)
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Humanos , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Aleitamento Materno , Saúde da Família , Período Pós-Parto , Fatores Sociodemográficos , Recém-Nascido , Zona Rural , Saúde Materno-Infantil , Sistema ImunitárioRESUMO
Introducción: La pandemia por la COVID-19 ha tenido efectos en la salud de los trabajadores sanitarios, dentro de ellos, los obstetras, pero se desconocen las consecuencias en este grupo ocupacional. Objetivo: Determinar las consecuencias de la COVID-19 en la salud física, psicológica y social de obstetras del Perú. Métodos: Estudio observacional, descriptivo, transversal, realizado a 511 obstetras asistenciales, en la primera línea de atención. Se aplicó un cuestionario en línea, previamente validado y confiable para medir las variables: características generales y de exposición a la COVID-19, salud física, salud psicológica y salud social. Los datos fueron analizados mediante estadística descriptiva. Resultados: 468 (91,6 %) obstetras son mujeres, 287 (56,2 %) de 40-59 años, 213 (41,7 %) casadas, 292 (57,1 %) laborando en el primer nivel de atención; 216 (42,3 %) se infectaron de la COVID-19, 165 (32,3 %) tuvieron cefalea, 127 (24,9 %) dolor de garganta y 121 (23,7 %) fatiga; 167 (77,3 %) requirieron tratamiento ambulatorio y 3 (1,3 %) cuidados intensivos. La obesidad reportada por 102 (20,0 %) obstetras fue la principal comorbilidad, seguida de hipertensión con 38 (7,4 %) y diabetes con 14 (2,7 %); 263 (51,5 %) reportaron síntomas depresivos y 464 (90,8 %) estrés; 238 (46,6 %) sintieron discriminación y 118 (23,1 %) llevaban la carga laboral del hogar en solitario. Conclusiones: La salud física, psicológica y social de los obstetras se afecta por la pandemia; genera trastornos de sobrepeso/ obesidad, depresión, estrés; así como discriminación y sobrecarga laboral en el hogar.
Introduction: The COVID-19 pandemic has had effects on the health of health workers, including obstetricians, but the consequences in this occupational group are unknown. Objective: Determine the consequences of COVID-19 on the physical, psychological and social health of obstetricians in Peru. Methods: Observational, descriptive, cross-sectional study, carried out on 511 attending obstetricians, in the first line of care. An online questionnaire, previously validated and reliable, was applied to measure the variables: general characteristics and exposure to COVID-19, physical health, psychological health, and social health. Data were analyzed using descriptive statistics. Results: 468 (91.6%) obstetricians are women, 287 (56.2%) aged 40-59 years, 213 (41.7%) married, 292 (57.1%) working at the first level of care; 216 (42.3%) were infected with COVID-19, 165 (32.3%) had headache, 127 (24.9%) sore throat and 121 (23.7%) fatigue; 167 (77.3%) required outpatient treatment and 3 (1.3%) intensive care. Obesity reported by 102 (20.0%) obstetricians was the main comorbidity, followed by hypertension with 38 (7.4%) and diabetes with 14 (2.7%); 263 (51.5%) reported depressive symptoms and 464 (90.8%) stress; 238 (46.6%) felt discrimination and 118 (23.1%) carried the workload of the home alone. Conclusions: The physical, psychological and social health of obstetricians is affected by the pandemic; generates disorders of overweight/obesity, depression, stress; as well as discrimination and work overload at home.
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High Intellectual Ability (HIA) is a differential manifestation of human intelligence with a neurobiological basis but which must express its high potential along the developmental trajectory through the covariation of endogenous (such as social competence) and exogenous modulating factors. The aim of the study is twofold: 1) to know, comparatively, the social competence of children with and without HIA, 2) to differentiate those social competences that could be protective or risk factors against the misuse of digital technologies. The Social Skills Improvement System-Rating Scales were administered to a sample of n = 70 learners (n = 35 with ICA, n = 35 with average intelligence) aged 11 to 16, analysing whether there are statistically significant differences in social skills and behavioural difficulties. Results show statistically significant differences in favour of participants with ICA in social skills (especially in: Responsibility, Co-operation and Self-Control) and better personal adjustment, with low incidence of internalising and externalising difficulties. We conclude and discuss the protective role of social skills in coping with complex interactive contexts such as the digital age and aggressions such as cyberbullying.
La Alta Capacidad intelectual (ACI) es una manifestación diferencial de la inteligencia humana, de base neurobiológica, pero que debe expresar su alto potencial a lo largo del desarrollo de la persona que la posee, mediante la covariación de factores moduladores endógenos (como la competencia social) y exógenos. El objetivo del trabajo es doble: 1) conocer, comparativamente la competencia social de menores con y sin ACI, 2) diferenciar aquellas competencias sociales que podrían ser factores protectores o de riesgo frente al mal uso de las tecnologías digitales. Se administra la Social Skills Improvement System-Rating Scales a una muestra de n = 70 aprendices (n = 35 con ACI, n = 35 con inteligencia promedio) de 11 a 16 años, analizando si existen diferencias estadísticamente significativas en habilidades sociales y en dificultades de conducta. Los resultados muestran diferencias estadísticamente significativas, a favor de los participantes con ACI en habilidades sociales (especialmente en: Responsabilidad, Cooperación y Autocontrol) y mejor ajuste personal, con baja incidencia de dificultades internalizantes y externalizantes. Se concluye y discute el rol protector de las habilidades sociales para afrontar contextos interactivos complejos como el derivado de la era digital y agresiones como el cyberbullying.
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Cyberbullying , Habilidades Sociais , Criança , Humanos , Comportamento Social , Adaptação Psicológica , CogniçãoRESUMO
Bladder cancer is a worldwide problem and improved therapies are urgently needed. In the search for newer strong antitumor compounds, herein, we present the study of three nitric oxide-releasing compounds and evaluate them as possible therapies for this malignancy. Bladder cancer cell lines T24 and 253J were used to evaluate the antiproliferative, antimigratory, and genotoxic effects of compounds. Moreover, we determined the NF-κB pathway inhibition, and finally, the survivin downregulation exerted by our molecules. The results revealed that compounds 1 and 3 exerted a high antiproliferative activity against bladder cancer cells through DNA damage and survivin downregulation. In addition, compound 3 reduced bladder cancer cell migration. We found that nitric oxide donors are promising molecules for the development of a new therapeutic targeting the underlying mechanisms of tumorigenesis and progression of bladder cancer.
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Antecedentes:La caries dental es una enfermedad que afecta a gran parte de la población mundial por lo que una vacuna para combatirla es el objetivo de este siglo, muchos investigadores están trabajando en vacunas de ADN con adyuvantes como: péptidos sintéticos, con subunidades no tóxicas de E. coli, con liposomas, microcápsulas y micropartículas, vacunas recombinadas; que prometen excelentes resultados en ensayos clínicos.Objetivo:Efectuar una descripción general de la evidencia disponible sobre la efectividad de las vacunas contra la caries dental.Materiales y métodos:La búsqueda inicial abarcó 1988 artículos, de los cuales se seleccionaron 23 para esta revisión, distribuidos 65.21% en Asia, 26.08% en Norteamérica, el 4.34% en Europa, 4.34% en Sudamérica. Se realizó una extensa búsqueda electrónica en las bases de datos: Pubmed, Proquest, Sage Journals, Ovid, Science Direct, Epistemonikos, Medline, Google Scholar, Cochrane, Scopus, Taylor and Francis online, Web of Science, Base, Springer, Research gate, Healey Library, Lilacs, Redalyc, Scielo, DialnetConclusión:Existen varias investigaciones realizadas que han brindado "potenciales" vacunas contra la caries dental, han demostrado eficacia inmunitaria mediante ensayos clínicos en ratones de laboratorio arrojando resultados prometedores, gran capacidad de producir respuestas inmunitarias, se ha puesto énfasis especial en la administración intranasal por sus beneficios y mínima citotoxicidad, sin embargo, existe escasa información referente a pruebas en humanos por lo que se debe redirigir esfuerzos y recursos en la búsqueda de la inmunización contra la caries dental.
Introduction:Dental caries is a disease that affects a large part of the world population so a vaccine to combat it is the goal of this century, many researchers are working on DNA vaccines with adjuvants such as synthetic peptides, liposomes, microcapsules, and microparticles, recombinant vaccines; which promise excellent results in clinical trials. Objective:To make an overview of the available evidence on the effectiveness of vaccines against dental caries. Materials and Methods:The initial search encompassed 1988 articles, of which 23 were selected for this review, distributed 65.21% in Asia, 26.08% in North America, 4.34% in Europe, 4.34% in South America. Sources:An extensive electronic search was performed in the databases: Pubmed, Proquest, Sage Journals, Ovid, Science Direct, Epistemonikos, Medline, Google Scholar, Cochrane, Scopus, Taylor, and Francis Online, Web of Science, Base, Springer, Research gate, Healey Library, Lilacs, Redalyc, Scielo, Dialnet. Conclusion:Several investigations have provided "potential" vaccines against dental caries, they have demonstrated immune efficacy through clinical trials in laboratory mice showing promising results, a great capacity to produce immune responses, special emphasis has been placed on intranasal administration due to its benefits and minimal cytotoxicity, however, there is scarce information regardinghuman trials, so efforts and resources should be redirected in the search for immunization against dental caries.
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Mucormycoses are rare but serious opportunistic fungal infections caused by filamentous organisms of the order Mucorales. Here we report the first molecular identification of Rhizopus oryzae (heterotypic synonym Rhizopus arrhizus), R. delemar, and Apophysomyces ossiformis as the etiological agents of three cases of severe mucormycosis in Honduras. Conventional microbiological cultures were carried out, and DNA was extracted from both clinical samples and axenic cultures. The ITS ribosomal region was amplified and sequenced. Molecular tools are suitable strategies for diagnosing and identifying Mucorales in tissues and cultures, especially in middle-income countries lacking routine diagnostic strategies.
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La erupción dentaria tiene inicio en las primeras fases de la odontogénesis y termina cuando el diente alcanza su posición funcional en el plano oclusal. La erupción de dientes primarios y la manifestación de los síntomas en niños, es un tema de amplio debate en la literatura. El objetivo del estudio es determinar si se presentan signos y síntomas durante la erupción de los dientes primarios según lo informado por padres y tutores de niños de 6 meses a 3 años. Los datos fueron recolectados a través de un cuestionario dirigido a 50 padres y cuidadores que acompañaban a niños de 6 meses a 3 años para el cuidado odontológico Plataforma Brasil. Hubo signos y síntomas que se describieron como irritabilidad, fiebre, diarrea, encías inflamadas, prurito gingival, salivación excesiva, insomnio, pérdida de apetito, dolor, llanto, estomatitis y sin síntomas reportados. Se puede concluir que la irritabilidad, la fiebre y la diarrea fueron los signos y síntomas más referidos por los padres y tutores.
A irrupção dentária tem início nos primórdios da odontogênese com a fase pré-irruptiva e termina quando o dente atinge a sua posição funcional no plano oclusal. A associação entre irrupção de dentes decíduos e a manifestação de sintomas em crianças é tema de amplo debate na literatura. O objetivo do estudo é determinar se há ocorrência de sinais e sintomas durante a irrupção de dentes decíduos segundo o relato dos pais e responsáveis de crianças de 6 meses a 3 anos de idade. Dados foram coletados através de questionário direcionado a 50 pais e cuidadores que acompanhavam crianças de 6 meses a 3 anos de idade para atendimento odontológico, os dados foram coletados mediante autorização em Termo de Consentimento Livre e Esclarecido e o estudo foi aprovado pelo comitê de ética da Plataforma Brasil. Verificou-se ocorrência de sinais e sintoma que foram descritos como irritabilidade, febre, diarreia, gengiva inchada, coceira gengival, salivação excessiva, insônia, perda de apetite, dor, choro, estomatite e nenhuma sintomatologia relatada. Pode-se concluir que a irritabilidade, febre e diarréia foram os sinais e sintomas mais relatados pelos pais e responsáreis
The dental eruption starts in the early odontogenesis with the pre-irruptive phase and ends when the tooth reaches its functional position in the occlusal plane. The eruption of primary teeth and the manifestation of symptoms in children is the subject of a wide debate in the literature. The purpose of the study is to determine whether signs and symptoms occur during the eruption of deciduous teeth according to the reports of parents and guardians of children aged 6 months to 3 years. Data were collected through a questionnaire directed to 50 parents and caregivers who accompanied children from 6 months to 3 years of age for dental care. Brazil Platform. There were signs and symptoms that were described as irritability, fever, diarrhea, swollen gums, gingival itching, excessive salivation, insomnia, loss of appetite, pain, crying, stomatitis and no reported symptoms. It can be concluded that irritability, fever and diarrhea were the signs and symptoms most reported by parents and guardians
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Lactente , Pré-Escolar , Dente Decíduo , Erupção Dentária , Oclusão Dentária , Sinais e Sintomas , Estomatite , Assistência Odontológica , Consentimento Livre e Esclarecido , OdontogêneseRESUMO
BACKGROUND AND AIM: Circulating amino acids are modified by sex, body mass index (BMI) and insulin resistance (IR). However, whether the presence of genetic variants in branched-chain amino acid (BCAA) catabolic enzymes modifies circulating amino acids is still unknown. Thus, we determined the frequency of two genetic variants, one in the branched-chain aminotransferase 2 (BCAT2) gene (rs11548193), and one in the branched-chain ketoacid dehydrogenase (BCKDH) gene (rs45500792), and elucidated their impact on circulating amino acid levels together with clinical, anthropometric and biochemical parameters. METHODS AND RESULTS: We performed a cross-sectional comparative study in which we recruited 1612 young adults (749 women and 863 men) aged 19.7 ± 2.1 years and with a BMI of 24.9 ± 4.7 kg/m2. Participants underwent clinical evaluation and provided blood samples for DNA extraction and biochemical analysis. The single nucleotide polymorphisms (SNPs) were determined by allelic discrimination using real-time polymerase chain reaction (PCR). The frequencies of the less common alleles were 15.2 % for BCAT2 and 9.83 % for BCKDH. The subjects with either the BCAT2 or BCKDH SNPs displayed no differences in the evaluated parameters compared with subjects homozygotes for the most common allele at each SNP. However, subjects with both SNPs had higher body weight, BMI, blood pressure, glucose, and circulating levels of aspartate, isoleucine, methionine, and proline than the subjects homozygotes for the most common allele (P < 0.05, One-way ANOVA). CONCLUSION: Our findings suggest that the joint presence of both the BCAT2 rs11548193 and BCKDH rs45500792 SNPs induces metabolic alterations that are not observed in subjects without either SNP.
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3-Metil-2-Oxobutanoato Desidrogenase (Lipoamida)/genética , Aminoácidos/sangue , Antígenos de Histocompatibilidade Menor/genética , Polimorfismo de Nucleotídeo Único , Proteínas da Gravidez/genética , Transaminases/genética , 3-Metil-2-Oxobutanoato Desidrogenase (Lipoamida)/metabolismo , Adolescente , Fatores Etários , Biomarcadores/sangue , Glicemia/análise , Pressão Sanguínea , Índice de Massa Corporal , Estudos Transversais , Feminino , Frequência do Gene , Estudos de Associação Genética , Homozigoto , Humanos , Masculino , México , Antígenos de Histocompatibilidade Menor/metabolismo , Fenótipo , Proteínas da Gravidez/metabolismo , Transaminases/metabolismo , Adulto JovemRESUMO
After the European colonization of the Americas, there was a dramatic population collapse of the Indigenous inhabitants caused in part by the introduction of new pathogens. Although there is much speculation on the etiology of the Colonial epidemics, direct evidence for the presence of specific viruses during the Colonial era is lacking. To uncover the diversity of viral pathogens during this period, we designed an enrichment assay targeting ancient DNA (aDNA) from viruses of clinical importance and applied it to DNA extracts from individuals found in a Colonial hospital and a Colonial chapel (16th-18th century) where records suggest that victims of epidemics were buried during important outbreaks in Mexico City. This allowed us to reconstruct three ancient human parvovirus B19 genomes and one ancient human hepatitis B virus genome from distinct individuals. The viral genomes are similar to African strains, consistent with the inferred morphological and genetic African ancestry of the hosts as well as with the isotopic analysis of the human remains, suggesting an origin on the African continent. This study provides direct molecular evidence of ancient viruses being transported to the Americas during the transatlantic slave trade and their subsequent introduction to New Spain. Altogether, our observations enrich the discussion about the etiology of infectious diseases during the Colonial period in Mexico.
The arrival of European colonists to the Americas, beginning in the 15th century, contributed to the spread of new viruses amongst Indigenous people. This led to massive outbreaks of disease, and millions of deaths that caused an important Native population to collapse. The exact viruses that caused these outbreaks are unknown, but smallpox, measles, and mumps are all suspected. During these times, traders and colonists forcibly enslaved and displaced millions of people mainly from the West Coast of Africa to the Americas. The cruel, unsanitary, and overcrowded conditions on ships transporting these people across the Atlantic contributed to the spread of infectious diseases onboard. Once on land, infectious diseases spread quickly, partly due to the poor conditions that enslaved and ndigenous people were made to endure. Native people were also immunologically naïve to the newly introduced pathogens, making them susceptible to severe or fatal outcomes. The new field of paleovirology may help scientists identify the viruses that were circulating in the first years of colonization and trace how viruses arrived in the Americas. Using next-generation DNA sequencing and other cutting-edge techniques, Guzmán-Solís et al. extracted and enriched viral DNA from skeletal remains dating back to the 16th century. These remains were found in mass graves that were used to bury epidemic victims at a colonial hospital and chapel in what is now Mexico City. The experiments identified two viruses, human parvovirus B19 and a human hepatitis B virus. These viral genomes were recovered from human remains of first-generation African people in Mexico, as well as an individual who was an Indigenous person. Although the genetic material of these ancient viruses resembled pathogens that originated in Africa, the study did not determine if the victims died from these viruses or another cause. On the other hand, the results indicate that viruses frequently found in modern Africa were circulating in the Americas during the slave trade period of Mexico. Finally, the results provide evidence that colonists who forcibly brought African people to the Americas participated in the introduction of viruses to Mexico. This constant influx of viruses from the old world, led to dramatic declines in the populations of Indigenous people in the Americas.
Assuntos
DNA Antigo/análise , Pessoas Escravizadas/história , Genoma Viral/genética , Vírus da Hepatite B/genética , Parvovirus B19 Humano/genética , População Negra/história , Vírus da Hepatite B/isolamento & purificação , Sequenciamento de Nucleotídeos em Larga Escala , História do Século XVI , História do Século XVII , História do Século XVIII , Humanos , Metagenômica , Parvovirus B19 Humano/isolamento & purificaçãoRESUMO
Adipokines, especially chemerin, can interact with cytokines and other molecules in inflammation. To date, there is insufficient information regarding a possible correlation between functional disability and chemerin and other pro-inflammatory molecules in rheumatoid arthritis (RA). To identify the association of functional disability with serum chemerin and other pro-inflammatory molecules, including other adipokines, cytokines and E-selectin, in patients with RA. Cross-sectional study. Assessment: disease activity (DAS28-ESR) and functional disability (HAQ-DI). We compared the adipokines (chemerin, leptin, adiponectin, resistin, and visfatin), cytokines (TNF-α, IL-6, IL-1ß, and IL-18) and E-selectin levels between RA with functional disability and RA non-disabled patients. Of 82 patients with RA, 43 (52%) had functional disability. The RA with functional disability group had higher chemerin (140 vs. 112 ng/mL, p = 0.007) than the non-disabled RA group. Chemerin correlated with the HAQ-DI (rho = 0.27, p = 0.02) and DAS28-ESR (rho = 0.21, p = 0.05). Severe activity correlated with IL-6 (rho = 0.33, p = 0.003) and E-selectin (rho = 0.23, p = 0.03) but not with disability. No other pro-inflammatory molecules correlated with HAQ-DI. High chemerin levels were associated with functional disability in RA, whereas no other molecules correlated with loss of function. These results encourage further studies assessing new roles of chemerin as a marker of impairment in RA.
Assuntos
Artrite Reumatoide/diagnóstico , Quimiocinas/sangue , Adulto , Idoso , Artrite Reumatoide/fisiopatologia , Biomarcadores/sangue , Estudos Transversais , Avaliação da Deficiência , Pessoas com Deficiência , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Índice de Gravidade de DoençaRESUMO
Resumen El uso de medios electrónicos como herramienta educativa es algo muy frecuente en la sociedad global del conocimiento, favorecido por el avance tecnológico. Se emplean nuevas alternativas en la práctica docente y en los procesos educativos, y las instituciones dedicadas a la educación médica no son la excepción. El objetivo de este trabajo fue establecer el uso de las tecnologías de la información y la comunicación (TIC) para el aprendizaje en estudiantes de medicina de la Universidad Autónoma de Tamaulipas, campus sur. Se siguió un estudio descriptivo, observacional y transversal. Los datos se recabaron a través de un cuestionario aplicado a 109 estudiantes. Del total de participantes, el 90.83 % contaba con computadora portátil, el 94.50 % con internet, ya sea en computadora o celular. El tiempo empleado diariamente usando las TIC para actividades educativas era entre 2 h y 4 h y el Google académico fue la base de datos más empleada para búsqueda de información. Por otro lado, se encontró que entre el 50 % y el 75 % de los encuestados tienen comprensión y dominio del idioma inglés. El uso de las TIC es de gran apoyo para los estudiantes, ya que les permite facilitar las búsquedas de información científica y médica, además de desarrollar competencias en el procesamiento y manejo de la información, para intercambiar información científica actualizada y continuar con su educación médica.
Abstract The use of electronic devices as educational tools is very frequent in the global knowledge society, which has been favored by current technological advancement. New alternatives are employed in teaching practice and in educational processes, and institutions dedicated to medical education are no exception. The objective of this work was to establish whether the use of information and communication technologies (ICT) for medical students' learning at the Autonomous University of Tamaulipas, Southern campus. A descriptive, observational and cross-sectional study was carried out. Data was collected through a questionnaire administered to 109 students. Of the total number of participants, 90.83 % had a laptop, 94.50 % had internet access, either via a computer or a mobile phone. The effective time spent daily using ICT for educational activities ranged between two and four hours, and Google Scholar was the most used database for information retrieval. On the other hand, it was found that between 50 % and 75 % of respondents have an understanding and command of the English language. The use of ICT's is very supportive for students, since it facilitates both, the scientific and medical information retrieval, as well as the development of competencies in the processing and management of information. This simplifies the exchange of updated scientific information to continue with their medical education.
RESUMO
In the development of cervical cancer (CC), the immune response plays an essential role, from the elimination of human papillomavirus (HPV) infection to the response against the tumor. For optimal function of the immune response, various factors are required, one of the most important being an adequate nutrition. The complex interaction between nutrients and microbiota maintains the immune system in homeostasis and in case of infection, it provides the ability to fight against pathogen invasion, as occurs in HPV infection. The purpose of this article is to describe the role of diet, food, and specific nutrients in the immune response from the onset of infection to progression to precancerous lesions and CC, as well as the role of diet and nutrition during oncological treatment. The immunomodulatory role of microbiota is also discussed. A detailed analysis of the evidence leads us to recommend a nutritional pattern very similar to the Mediterranean diet or the prudent diet for an optimal immune response. Moreover, pre- and probiotics favorably modulate the microbiota and induce preventive and therapeutic effects against cancer.
Assuntos
Imunidade , Estado Nutricional , Infecções por Papillomavirus , Neoplasias do Colo do Útero , Dieta , Feminino , Microbioma Gastrointestinal , Humanos , Infecções por Papillomavirus/complicações , Infecções por Papillomavirus/imunologia , Neoplasias do Colo do Útero/imunologia , Neoplasias do Colo do Útero/terapiaRESUMO
The 'red complex' is an aggregate of three oral bacteria (Tannerella forsythia, Porphyromonas gingivalis and Treponema denticola) responsible for severe clinical manifestation of periodontal disease. Here, we report the first direct evidence of ancient T.forsythia DNA in dentin and dental calculus samples from archaeological skeletal remains that span from the Pre-Hispanic to the Colonial period in Mexico. We recovered twelve partial ancient T. forsythia genomes and observed a distinct phylogenetic placement of samples, suggesting that the strains present in Pre-Hispanic individuals likely arrived with the first human migrations to the Americas and that new strains were introduced with the arrival of European and African populations in the sixteenth century. We also identified instances of the differential presence of genes between periods in the T. forsythia ancient genomes, with certain genes present in Pre-Hispanic individuals and absent in Colonial individuals, and vice versa. This study highlights the potential for studying ancient T. forsythia genomes to unveil past social interactions through analysis of disease transmission. Our results illustrate the long-standing relationship between this oral pathogen and its human host, while also unveiling key evidence to understand its evolutionary history in Pre-Hispanic and Colonial Mexico. This article is part of the theme issue 'Insights into health and disease from ancient biomolecules'.
Assuntos
Genoma Bacteriano , Infecções por Bactérias Gram-Negativas/história , Periodontite/história , Tannerella forsythia/genética , Arqueologia , Genômica , Infecções por Bactérias Gram-Negativas/microbiologia , História do Século XV , História do Século XVI , História do Século XVII , História do Século XVIII , História Antiga , História Medieval , Humanos , México , Periodontite/microbiologiaRESUMO
ABSTRACT In the development of cervical cancer (CC), the immune response plays an essential role, from the elimination of human papillomavirus (HPV) infection to the response against the tumor. For optimal function of the immune response, various factors are required, one of the most important being an adequate nutrition. The complex interaction between nutrients and microbiota maintains the immune system in homeostasis and in case of infection, it provides the ability to fight against pathogen invasion, as occurs in HPV infection. The purpose of this article is to describe the role of diet, food, and specific nutrients in the immune response from the onset of infection to progression to precancerous lesions and CC, as well as the role of diet and nutrition during oncological treatment. The immunomodulatory role of microbiota is also discussed. A detailed analysis of the evidence leads us to recommend a nutritional pattern very similar to the Mediterranean diet or the prudent diet for an optimal immune response. Moreover, pre- and probiotics favorably modulate the microbiota and induce preventive and therapeutic effects against cancer.
Assuntos
Humanos , Feminino , Neoplasias do Colo do Útero/imunologia , Neoplasias do Colo do Útero/terapia , Estado Nutricional , Infecções por Papillomavirus/complicações , Infecções por Papillomavirus/imunologia , Imunidade , Dieta , Microbioma GastrointestinalRESUMO
An important goal in the management of systemic lupus erythematosus (SLE) is the prediction of relapses. This study assesses whether anti-nucleosome antibodies (anti-NCS) increase the risk of renal relapse in inactive SLE. A prospective cohort of 115 patients with inactive SLE (M-SLEDAI ≤ 2) were followed for 12 months to assess the development of relapse (increase of M-SLEDAI ≥ 4) and specific renal flare (renal SLEDAI ≥ 4). At baseline, we identified potential risk factors for relapse, including anti-NCS. At baseline, 18 (16%) of the 115 patients with inactive SLE were anti-NCS positive. At the 12-month follow-up, anti-NCS-positive patients had a higher incidence of renal relapse compared to anti-NCS-negative patients (38.9% vs 13.4%, respectively). In Cox regression analysis, after adjusting for age, disease duration, anti-dsDNA, and immunosuppressive drugs, the presence of anti-NCS positivity at baseline increased the risk of renal relapse (HR: 5.31, 95% CI 2.03-13.92). Nevertheless, there were no differences in the incidence of other relapses in anti-NCS-positive versus anti-NCS-negative. Our results indicate that in inactive SLE, anti-NCS determination can be useful for identifying patients with a higher risk of developing renal relapse. Interestingly, this study identified that continued use of oral immunosuppressive therapy in patients with inactive SLE can reduce the risk of renal relapse.