RESUMO
Automated indirect immunoperoxidase (avidin-biotin complex) staining using monoclonal antibody #5DF12-3B6, directed against rabies N-protein, was used to detect rabies antigen in tissue samples from animals either naturally or experimentally infected with rabies. This monoclonal antibody recognized all 16 strains of rabies virus tested, as well as rabies-related lyssaviruses including Duvenhage, Lagos Bat, and Mokola. The sample infected with Mokola virus initially showed only weak staining, however, deletion of protease digestion resulted in stronger stain uptake. The test was sensitive and specific, correctly identifying rabies antigen in all but one of the samples tested (37/38), and no apparent staining in any of the negative samples tested (23/23). Tissues from 16 mammalian species were tested, including one rabies infected human tissue sample. The utility of the immunoperoxidase staining method described in this study lies in the ability of one monoclonal to recognize a broad spectrum of lyssaviruses in formalin-fixed tissues.
Assuntos
Raiva , Vírus da Raiva , Lyssavirus , Anticorpos MonoclonaisRESUMO
La estomatitis vesicular es una enfermedad que afecta bovinos, ovinos, porcinos, caprinos y equinos clínicamente indistinguible de la fiebre aftosa. En Brasil fue diagnosticada por primera vez en 1964 en los estados de Alagoas y Pernambuco. En este trabajo se hace un relato histórico a partir de los resultados de diagnósticos de los virus tipos Indiana-2 e Indiana-3 realizados durante los años 1964-1996 en el Centro Panamericano de Fiebre Aftosa.
Assuntos
Vírus da Estomatite Vesicular Indiana , Zoonoses , Estomatite VesicularRESUMO
Se describe um método práctico basado en el uso de un detergente no iónico, que se puede remover por diálisis para la extración de las glicoproteínas del virus de la estomatitis vesicular con el objeto de ser usadas para la identificación de anticuerpos por la técnica de ELISA.