RESUMO
Phytophthora capsici from seven provinces of China were investigated for their mating type, hyphal growth, zoospore production, and virulence. All of the morphological characteristics and the results of polymerase chain reaction confirmed that these isolates were indeed Phytophthora capsici. The test of mating type showed that the mating types of 19 representative isolates from China varied. The hyphal growth and the amount of zoospores produced from these isolates differed and there was no evident relationship between them, which indicated the existence of genetic diversity among the isolates in China. Also, the isolates that were more virulent on the pepper cultivars that we checked produced more zoospores than other isolates.
Assuntos
Phytophthora/genética , Capsicum/microbiologia , Phytophthora/isolamento & purificação , Phytophthora/patogenicidade , Phytophthora/fisiologia , Reprodução/genética , Esporos/genética , Esporos/fisiologia , Virulência/genéticaRESUMO
We amplified S14R protein gene cDNA of porcine, cloned it into a prokaryotic expression plasmid, and expressed it in Escherichia coli. A pair of primers was designed based on the cDNA sequence of the porcine S14R gene in GenBank. The target gene fragment from porcine liver tissue was amplified by RT-PCR. Confirmed by auto-sequencing, the target gene fragment was subcloned into an expression vector of pET28a. The pET28a-S14R construct was subsequently transformed into E. coli BL21 (DE3). This construct was verified by restriction endonuclease digestion and sequencing. Using isopropyl ß-D-1-thiogalactopyranoside induction, a new recombinant protein with the expected relative molecular mass of 24 kDa appeared. The result was identified by SDS-PAGE electrophoresis. Porcine S14R includes 549bp (GenBank No. JN793537), with an open reading frame of 549 bp coding 182 amino acids.