RESUMO
Numerous studies have evaluated the association between Arg399Gln polymorphism of DNA repair gene XRCC1 and cervical carcinoma risk. However, the specific association is still controversial. To assess the relationship between XRCC1 Arg399Gln polymorphism and cervical carcinoma, we conducted a comprehensive meta-analysis of 10 case-control studies with 2051 cervical carcinoma cases and 2919 controls. Meta-analysis results based on all the studies showed a significant association between XRCC1 Arg399Gln polymorphism and cervical carcinoma risk (GlnGln vs ArgArg: OR = 1.29, 95%CI = 0.90-1.85; GlnGln vs ArgGln: OR = 1.15, 95%CI = 0.93-1.43; the dominant model: OR = 0.80, 95%CI = 0.66-0.99; the recessive model: OR = 1.18, 95%CI = 0.93-1.49). In the subgroup analysis by ethnicity, the results also showed significant association between XRCC1 Arg399Gln polymorphism and susceptibility to cervical carcinoma in both Caucasian and Asian populations. The Arg399Gln polymorphism in the XRCC1 gene may be related to the increased risk of cervical carcinoma. Conclusive evidence on the effects of the variants in cervical carcinoma should be addressed in further studies.
Assuntos
Alelos , Proteínas de Ligação a DNA/genética , Estudos de Associação Genética , Predisposição Genética para Doença , Polimorfismo de Nucleotídeo Único , Neoplasias do Colo do Útero/genética , Substituição de Aminoácidos , Estudos de Casos e Controles , Feminino , Genótipo , Humanos , Razão de Chances , Viés de Publicação , Proteína 1 Complementadora Cruzada de Reparo de Raio-XRESUMO
Oxygen therapy is essential for the treatment of some neonatal critical care conditions but its extrapulmonary effects have not been adequately investigated. We therefore studied the effects of various oxygen concentrations on intestinal epithelial cell function. In order to assess the effects of hyperoxia on the intestinal immunological barrier, we studied two physiological changes in neonatal rats exposed to hyperoxia: the change in intestinal IgA secretory component (SC, an important component of SIgA) and changes in intestinal epithelial cells. Immunohistochemistry and Western blot were used to detect changes in the intestinal tissue SC of neonatal rats. To detect intestinal epithelial cell growth, cells were counted, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Giemsa staining were used to assess cell survival. Immunohistochemistry was used to determine SC expression. The expression of intestinal SC in neonatal rats under hyperoxic conditions was notably increased compared with rats inhaling room air (P < 0.01). In vitro, 40 percent O2 was beneficial for cell growth. However, 60 percent O2 and 90 percent O2 induced rapid cell death. Also, 40 percent O2 induced expression of SC by intestinal epithelial cells, whereas 60 percent O2did not; however, 90 percent O2 limited the ability of intestinal epithelial cells to express SC. In vivo and in vitro, moderate hyperoxia brought about increases in intestinal SC. This would be expected to bring about an increase in intestinal SIgA. High levels of SC and SIgA would serve to benefit hyperoxia-exposed individuals by helping to maintain optimal conditions in the intestinal tract.
Assuntos
Animais , Feminino , Humanos , Ratos , Células Epiteliais/citologia , Hiperóxia/metabolismo , Imunoglobulina A Secretora/metabolismo , Mucosa Intestinal/citologia , Animais Recém-Nascidos , Western Blotting , Proliferação de Células , Células Epiteliais/metabolismo , Imuno-Histoquímica , Ratos WistarRESUMO
Oxygen therapy is essential for the treatment of some neonatal critical care conditions but its extrapulmonary effects have not been adequately investigated. We therefore studied the effects of various oxygen concentrations on intestinal epithelial cell function. In order to assess the effects of hyperoxia on the intestinal immunological barrier, we studied two physiological changes in neonatal rats exposed to hyperoxia: the change in intestinal IgA secretory component (SC, an important component of SIgA) and changes in intestinal epithelial cells. Immunohistochemistry and Western blot were used to detect changes in the intestinal tissue SC of neonatal rats. To detect intestinal epithelial cell growth, cells were counted, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Giemsa staining were used to assess cell survival. Immunohistochemistry was used to determine SC expression. The expression of intestinal SC in neonatal rats under hyperoxic conditions was notably increased compared with rats inhaling room air (P < 0.01). In vitro, 40% O2 was beneficial for cell growth. However, 60% O2 and 90% O2 induced rapid cell death. Also, 40% O2 induced expression of SC by intestinal epithelial cells, whereas 60% O2 did not; however, 90% O2 limited the ability of intestinal epithelial cells to express SC. In vivo and in vitro, moderate hyperoxia brought about increases in intestinal SC. This would be expected to bring about an increase in intestinal SIgA. High levels of SC and SIgA would serve to benefit hyperoxia-exposed individuals by helping to maintain optimal conditions in the intestinal tract.