RESUMO

Rhododendron possesses valuable horticultural and medicinal properties. However, the genetic studies have been hindered due to the lack of genetic markers. Based on RNA-seq, large-scale molecular markers were developed from four Rhododendron species endemic to Dabie Mountains (central China): R. fortunei (5.25 Gb; SSRs, 12,756, one/2.37 kb, 147 types; SNPs, 38,313; InDels, 3,174), R. simsii (5.80 Gb; SSRs, 13,294, one/2.58 kb, 167 types; SNPs, 136,590; InDels, 6,258), R. mariesii (6.53 Gb; SSRs, 15,724, one/2.51 kb, 170 types; SNPs, 44,942; InDels, 4,126), and R. molle (4.35 Gb; SSRs, 10,214, one/2.49 kb, 110 types; SNPs, 77,829; InDels, 3,416). Di-nucleotide repeats were the main type (59.126%-64.314%), and AG/CT repeat (55.18%-61.22%) was the most. In particular, 89 species-specific types had been found. Furthermore, C:G→T:A mutation was the main SNP type (30.475%-34.99%). However, C:G→G:C mutation was the least type in R. fortunei, while T:A→G:C mutation was the least in the other three species. InDels with length of 3 nt was most in R. fortunei, but 1 nt InDels were the main type in the other three species. Twelve microsatellite markers developed from R. simsii reveled high genetic diversity in the four populations, and heterozygote excess was observed. This research would benefit the genetic study, molecular marker-assisted selection, and breeding studies in Rhododendron species.

RESUMO

Descrevemos um caso de infecçäo por HTLV-I associado a mielopatia, em mulher de 50 anos, na Nigeria. A paciente apresentou fraqueza progressiva dos membros inferiores e posteriormente incapacidade para andar. A presença de anticorpo HTLV-I no plasma coletado da paciente foi repetidamente detectada pelos ensaios imunoenzimaticos (Abbott HTLV-I EIA e Coulter SELECT-HTLV I/II) e confirmada pela tecnica de Western Blot. Adicionalmente amplificou-se o DNA do HTLV-I a partir do DNA genomico isolado das celulas mononucleares do sangue periferico da paciente através da técnica de PCR. Este achado e significativo sendo o primeiro relato de associaçäo de HTLV-I com mielopatia, na Nigeria

Assuntos

Humanos , Masculino , Feminino , Adulto , Anticorpos Antideltaretrovirus/isolamento & purificação , Leucócitos Mononucleares/imunologia , Paraparesia Espástica Tropical/diagnóstico , Amplificação de Genes/métodos , Western Blotting , Infecções por HTLV-I/etiologia , Nigéria , Reação em Cadeia da Polimerase