RESUMO

Background: The dissemination of polymyxin resistance represents a significant threat to public health. Materials & methods: Sequence-based typing was performed by 53 mcr-1 Escherichia coli isolates using fumC/fimH (CH) genes to characterize clones spreading from pig farming. Furthermore, 12 isolates had their whole genome sequenced for phylogenetic study. Results: The isolates were classified into 22 distinct CH types, and two novel CH types (CH41-1578 and CH4-1579) and one sequence type (ST12652) was also described. According to phylogenetic study, both multilocus sequence typing and CH methods grouped the isolates similarly. Conclusion: Our findings suggest that the dissemination of the mcr-1 gene in pig farming has occurred mainly by horizontal gene transfer, and CH typing proved to be a good tool to characterize E. coli clones.

Assuntos

Infecções por Escherichia coli , Proteínas de Escherichia coli , Animais , Suínos , Escherichia coli , Fazendas , Infecções por Escherichia coli/veterinária , Infecções por Escherichia coli/genética , Alelos , Filogenia , Proteínas de Escherichia coli/genética , Tipagem de Sequências Multilocus , Antibacterianos/farmacologia , Colistina/farmacologia , Testes de Sensibilidade Microbiana , Adesinas de Escherichia coli/genética , Proteínas de Fímbrias/genética

Assuntos

Escherichia coli/genética , Polimixinas , Animais , Brasil , Infecções por Escherichia coli , Humanos , Aves Domésticas