RESUMO

The protein nutrition of dairy cows is of great importance because of its direct influence on milk production, reproductive efficiency, and feeding cost. Eight first-lactation Holstein cows were randomly assigned to two contemporary 4 × 4 Latin squares in a 2 × 2 factorial design to evaluate the effects of replacing soybean meal with yeast-derived microbial protein (YMP) as a protein source (0% or 1.5% of dry matter (DM)) and its combination with slow-release urea (SRU; 0% or 0.75% of DM) on DM intake and milk production and composition, as well as blood parameters and nitrogen balance. Each experimental period lasted 28 days, with 21 days of adaptation and 7 days of data collection. The diets were formulated to attend the nutritional recommendations of the National Research Council and consisted of 49% forage (47% corn silage and 2% Tifton hay) and 51% concentrate, with 16.8% CP and 1.6 Mcal net energy for lactation/kg DM. For diets without YMP, the inclusion of SRU decreased DM intake, milk production as well as N intake and balance, but did not affect efficiency of production, milk composition or most of blood parameters. On the contrary, for diets with YMP, DM intake and milk production were increased by inclusion of SRU, while minor effects were observed for milk efficiency and composition, blood parameters as well as N intake, excretion and balance. When diets with SRU were compared, the inclusion of YMP increased DM intake, 4% fat-corrected milk, and N intake and balance (P<0.05), with no differences in milk production (kg/day), milk energy, efficiency of milk production or most of the blood parameters. For diets without SRU, YMP inclusion decreased DM intake, milk production, milk energy, N intake, fecal N and N balance (P<0.05), with no effects on milk efficiency and composition, or most of blood parameters. In conclusion, the use of YMP, SRU or both as partial substitutes of soybean meal in the diet of lactating cows has no negative effects on productivity parameters.

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Thirty six Nellore steers, with an average initial age and weight of 20 months and 360 kg, were fed the same diet in three levels of dry matter intake: ad libitum or in two restriction levels 75g DM/kg BW0.75 or 60g DM/kg BW0.75 during 58 days (Phase 1) to evaluate the effects of feed restriction on weight gain, feed efficiency, diet digestibility, and composition of weight gain in restriction and re-alimentation phases. All steers were fed ad libitum for 78 days (Phase 2). Body composition was estimated with the marker deuterium oxide, which allowed repeated measurements of the same animal and studying its response to re-alimentation. Average daily gain in the restriction phase reflected different weight gain rates provided by feed levels of 0.288 kg, 0.656 kg, and 1.169 kg, respectively for 60g DM/ BW0.75, 75g DM/ BW0.75 and ad libitum. Feed efficiency increased according to feed intake level in Phase 1 and no differences between treatments were observed in Phase 2. Dry matter digestibility did not differ between treatments in both Phase 1 and Phase 2; however, it differed between phases for the same treatment with 78.8% in Phase 1, and with 68.7% in Phase 2 for the treatment with 60 g DM/BW0.75, and 77.8% in Phase 1 and 71.3% in Phase 2 for the treatment with 75 g DM/BW0.75. The deposition of empty weight gain, daily water deposition, ether extract, protein, ash, and energy increased with a higher feed intake level in Phase 1. In Phase 2, no difference occurred between the diet intake levels for empty weight gain, chemical components deposition, and retained energy in the gain. Diet digestibility decreased in Nellore steers fed ad libitum after the period of feed restriction, regardless of the previous restriction level to which the animals were submitted.(AU)

Trinta e seis novilhos Nelore, com média de peso e idade inicial de 360 kg e 20 meses, foram alimentados com a mesma dieta em três níveis de ingestão de material seca: à vontade ou em dois níveis de restrição alimentar quantitativa 75g MS/kg PV0,75 ou 60g MS/kg PV0,75 durante 58 dias (Fase 1) para avaliar os efeitos da restrição alimentar no ganho de peso, eficiência alimentar, digestibilidade da dieta e na composição do ganho de peso. Todos os novilhos foram alimentados ad libitum por 78 dias (Fase 2). A composição corporal foi estimada com o marcador óxido de deutério, o qual permite repetir a avaliação no mesmo animal e estudar as respostas à realimentação. O ganho médio diário na fase de restrição alimentar refletiu as diferentes taxas de ganho de peso proporcionadas pelos níveis de alimentação, sendo 0,288 kg, 0,656 kg e 1.169 kg, respectivamente para 60g MS/PV0,75, 75g MS/PV0,75 e ad libitum. A eficiência alimentar variou de acordo com o nível de ingestão de alimentos na Fase 1 e não foram observadas diferenças entre tratamentos na Fase 2. A digestibilidade da matéria seca não diferiu entre tratamentos tanto na Fase 1 como na Fase 2, mas foi diferente entre as Fases para o mesmo tratamento com 78,8% na Fase 1 e 68,7% na Fase 2 para o tratamento 60 g MS/PV0,75 e 77,8% na Fase 1 e 71,3% na Fase 2 para o tratamento 75 g MS/PV0,75. A composição do ganho de peso vazio, a deposição diária de água, extrato etéreo, proteína, cinzas e energia aumentaram de acordo com o nível de ingestão de alimentos na Fase1. Na Fase 2, não houve diferença entre os níveis de ingestão da dieta para ganho de peso vazio, deposição dos componentes químicos e energia retida no ganho. Novilhos Nelore alimentados ad libitum após período de restrição alimentar tiveram a digestibilidade da dieta diminuída, independentemente do...(AU)

Assuntos

RESUMO

Blood parameters of 18 Nellore steers submitted to high concentrate diet in two sequential feeding regimes (phase 1: restriction and phase 2: ad libitum feeding) were evaluated. In the first phase, steers were fed for 56 days three intake levels of dry mat-ter (ad libitum, 75 g dry matter (DM)/kg metabolic body weight (BW0.75) or 60 g DM/kg BW(0.75). In the second phase, from the 57th day on feed, animals fed 60 g DM/kg BW(0.75) and 75 g DM/kg BW(0.75) were fed ad libitum until the 136th day. In the 8th, 27th, 55th (first phase), 71st, 84th, 112th and 132nd (second phase) day of experimental period, blood samples were collected and insulin-like growth factor I (IGF-I), triiodothyronine (T3), thyroxine (T4), glucose, plasma ureic nitrogen (PUN) and total protein (TP) were determined. In the first phase IGF-I, T4, glucose and total protein concentration was not different among treat-ments (P>0.05) and there was no interaction between treatment and time (P>0.05). There was an interaction among treatments and time on T3 and plasma ureic nitrogen (P<0.01). In the second phase IGF-I, T3, T4, glucose and total protein concentration was not different among treatments and there was no interaction between treatment and time (P>0.05). For plasma ureic nitrogen concentration there was an interaction among treatments and time (P=0.02). Lower levels of diet intake can be used to improve animal performance in feedlot.(AU)

Foram avaliados parâmetros sanguíneos de 18 novilhos Nelore submetidos a dois regimes sequenciais de alimentação (fase 1: restrição e fase 2: alimento ad libitum) com dieta de alto teor de concentrado. Na primeira fase, os animais foram alimentados por 56 dias em três níveis de ingestão de matéria seca (MS) representando os tratamentos: ad libitum, 75 g MS/kg peso corporal metabólico (PV0,75) e 60 g MS/kg PV(0,75). Na segunda fase, a partir do 57º dia de confinamento, os animais alimentados com 60 g MS/kg PV(0,75) e 75 g MS/kg PV(0,75) receberam alimentação ad libitum até o 136º dia. No 8º, 27º, 55º, primeira fase, e 71º, 84º, 112º e 132º dia de confinamento, segunda fase, foram colhidas amostras de sangue para determinação das concentrações de fator de crescimento semelhante à insulina I (IGF-I), triiodotironina (T3), tiroxina (T4), glicose, nitrogênio ureico plasmático e proteína total. No período de restrição alimentar as concentrações plasmáticas de IGF-I, T4, glicose e proteína total não foram diferentes entre os tratamentos (P>0,05) e não houve interação entre tratamento e tempo (P>0,05). Foi observada interação entre tratamento e tempo para a concentração plasmática de T3 e nitrogênio ureico no plasma (P<0,01). No período de alimento ad libitum as concentrações de IGF-I, T3, T4, glicose e proteína total não foram diferentes entre os tratamentos e não houve interação entre tratamento e tempo (P>0,05). Para a concentração de nitrogênio ureico no plasma houve interação entre os fatores tratamentos e tempo (P=0,02). Níveis mais baixos de ingestão de dieta podem ser usados para melhorar o desempenho animal em confinamento.(AU)

Assuntos

RESUMO

Blood parameters of 18 Nellore steers submitted to high concentrate diet in two sequential feeding regimes (phase 1: restriction and phase 2: ad libitum feeding) were evaluated. In the first phase, steers were fed for 56 days three intake levels of dry mat-ter (ad libitum, 75 g dry matter (DM)/kg metabolic body weight (BW0.75) or 60 g DM/kg BW(0.75). In the second phase, from the 57th day on feed, animals fed 60 g DM/kg BW(0.75) and 75 g DM/kg BW(0.75) were fed ad libitum until the 136th day. In the 8th, 27th, 55th (first phase), 71st, 84th, 112th and 132nd (second phase) day of experimental period, blood samples were collected and insulin-like growth factor I (IGF-I), triiodothyronine (T3), thyroxine (T4), glucose, plasma ureic nitrogen (PUN) and total protein (TP) were determined. In the first phase IGF-I, T4, glucose and total protein concentration was not different among treat-ments (P>0.05) and there was no interaction between treatment and time (P>0.05). There was an interaction among treatments and time on T3 and plasma ureic nitrogen (P0.05). For plasma ureic nitrogen concentration there was an interaction among treatments and time (P=0.02). Lower levels of diet intake can be used to improve animal performance in feedlot.

Foram avaliados parâmetros sanguíneos de 18 novilhos Nelore submetidos a dois regimes sequenciais de alimentação (fase 1: restrição e fase 2: alimento ad libitum) com dieta de alto teor de concentrado. Na primeira fase, os animais foram alimentados por 56 dias em três níveis de ingestão de matéria seca (MS) representando os tratamentos: ad libitum, 75 g MS/kg peso corporal metabólico (PV0,75) e 60 g MS/kg PV(0,75). Na segunda fase, a partir do 57º dia de confinamento, os animais alimentados com 60 g MS/kg PV(0,75) e 75 g MS/kg PV(0,75) receberam alimentação ad libitum até o 136º dia. No 8º, 27º, 55º, primeira fase, e 71º, 84º, 112º e 132º dia de confinamento, segunda fase, foram colhidas amostras de sangue para determinação das concentrações de fator de crescimento semelhante à insulina I (IGF-I), triiodotironina (T3), tiroxina (T4), glicose, nitrogênio ureico plasmático e proteína total. No período de restrição alimentar as concentrações plasmáticas de IGF-I, T4, glicose e proteína total não foram diferentes entre os tratamentos (P>0,05) e não houve interação entre tratamento e tempo (P>0,05). Foi observada interação entre tratamento e tempo para a concentração plasmática de T3 e nitrogênio ureico no plasma (P0,05). Para a concentração de nitrogênio ureico no plasma houve interação entre os fatores tratamentos e tempo (P=0,02). Níveis mais baixos de ingestão de dieta podem ser usados para melhorar o desempenho animal em confinamento.

Assuntos

RESUMO

Thirty six Nellore steers, with an average initial age and weight of 20 months and 360 kg, were fed the same diet in three levels of dry matter intake: ad libitum or in two restriction levels 75g DM/kg BW0.75 or 60g DM/kg BW0.75 during 58 days (Phase 1) to evaluate the effects of feed restriction on weight gain, feed efficiency, diet digestibility, and composition of weight gain in restriction and re-alimentation phases. All steers were fed ad libitum for 78 days (Phase 2). Body composition was estimated with the marker deuterium oxide, which allowed repeated measurements of the same animal and studying its response to re-alimentation. Average daily gain in the restriction phase reflected different weight gain rates provided by feed levels of 0.288 kg, 0.656 kg, and 1.169 kg, respectively for 60g DM/ BW0.75, 75g DM/ BW0.75 and ad libitum. Feed efficiency increased according to feed intake level in Phase 1 and no differences between treatments were observed in Phase 2. Dry matter digestibility did not differ between treatments in both Phase 1 and Phase 2; however, it differed between phases for the same treatment with 78.8% in Phase 1, and with 68.7% in Phase 2 for the treatment with 60 g DM/BW0.75, and 77.8% in Phase 1 and 71.3% in Phase 2 for the treatment with 75 g DM/BW0.75. The deposition of empty weight gain, daily water deposition, ether extract, protein, ash, and energy increased with a higher feed intake level in Phase 1. In Phase 2, no difference occurred between the diet intake levels for empty weight gain, chemical components deposition, and retained energy in the gain. Diet digestibility decreased in Nellore steers fed ad libitum after the period of feed restriction, regardless of the previous restriction level to which the animals were submitted.

Trinta e seis novilhos Nelore, com média de peso e idade inicial de 360 kg e 20 meses, foram alimentados com a mesma dieta em três níveis de ingestão de material seca: à vontade ou em dois níveis de restrição alimentar quantitativa 75g MS/kg PV0,75 ou 60g MS/kg PV0,75 durante 58 dias (Fase 1) para avaliar os efeitos da restrição alimentar no ganho de peso, eficiência alimentar, digestibilidade da dieta e na composição do ganho de peso. Todos os novilhos foram alimentados ad libitum por 78 dias (Fase 2). A composição corporal foi estimada com o marcador óxido de deutério, o qual permite repetir a avaliação no mesmo animal e estudar as respostas à realimentação. O ganho médio diário na fase de restrição alimentar refletiu as diferentes taxas de ganho de peso proporcionadas pelos níveis de alimentação, sendo 0,288 kg, 0,656 kg e 1.169 kg, respectivamente para 60g MS/PV0,75, 75g MS/PV0,75 e ad libitum. A eficiência alimentar variou de acordo com o nível de ingestão de alimentos na Fase 1 e não foram observadas diferenças entre tratamentos na Fase 2. A digestibilidade da matéria seca não diferiu entre tratamentos tanto na Fase 1 como na Fase 2, mas foi diferente entre as Fases para o mesmo tratamento com 78,8% na Fase 1 e 68,7% na Fase 2 para o tratamento 60 g MS/PV0,75 e 77,8% na Fase 1 e 71,3% na Fase 2 para o tratamento 75 g MS/PV0,75. A composição do ganho de peso vazio, a deposição diária de água, extrato etéreo, proteína, cinzas e energia aumentaram de acordo com o nível de ingestão de alimentos na Fase1. Na Fase 2, não houve diferença entre os níveis de ingestão da dieta para ganho de peso vazio, deposição dos componentes químicos e energia retida no ganho. Novilhos Nelore alimentados ad libitum após período de restrição alimentar tiveram a digestibilidade da dieta diminuída, independentemente do...

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RESUMO

Immunocastration (ImC) has been proposed as an animal welfare-friendly alternative to reduce sexual and aggressive behavior and to increase carcass fat deposition with positive effects on meat quality. The ß-adrenergic agonists (ß-AA) are known as repartitioning agents that acts increasing lean tissue deposition. The combined use of these technologies can positively affect meat quality and increase retail cuts yield. Thus, this research was conducted to evaluate the combined effects of ImC and ß-AA (zilpaterol hydrochloride (ZH) and ractopamine hydrochloride (RH)) on retail cuts, bones, and fat trim of feedlot finished Bos indicus (Nellore) cattle. No interaction was observed between sexual condition and diet for any trait. The ImC decreased cold carcass, hindquarter (HQ), forequarter (FQ) and combined brisket, short ribs and flank (BSF) weights. The ImC also showed smaller weights of retail cuts and bones on the HQ and on the FQ than non-castrated (NoC). Fat trim weights did not differ from ImC and NoC. The most of subprimal cuts were heavier in NoC than in ImC. Feeding ß-AA did not affect cold carcass weight; however, animals fed ZH had higher weights of HQ and retail cuts in HQ when compared with RH and control (CO) group, with no differences between RH and CO for both traits. The weights of FQ, BSF, retail cuts in FQ, as well as bones and fat trimmings were not affected by ß-AA. In summary, ImC decreases carcass and retail cut weights, whereas ZH supplementation leads to an improvement in carcass lean tissue and retail cuts.

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RESUMO

ß-Adrenergic agonists (ß-AA) are non-hormonal growth promoters which promote muscle hypertrophy in supplemented animals. The effects of two ß-AA in combination with the immunocastration technique on the performance and carcass traits were evaluated using 96 feedlot Nellore males in a randomized complete block design with two sex conditions (immunocastrated (IC) v. non-castrated (NC)) and three treatments: CON (no ß-agonists added), RH (300 mg of ractopamine hydrochloride/day, for 33 days) or ZH (80 mg of zilpaterol·hydrochloride animal/day for 30 days, removed 3 days for required withdrawal period). The trial was carried for 100 days where in the first 70 days animals did not receive ß-AA (phase 1) and during the last 30 days they were treated with ß-AA (phase 2). The performance and ultrasound measurements of longissimus muscle area (LMA), backfat thickness (BFT) and rump fat thickness (RFT) were evaluated in both phases. No sex condition v. treatment interactions were observed for any trait. The NC animals had higher average daily gain (ADG) and final BW than the IC animals, but they did not differ in dry matter intake (DMI) and feed efficiency (gain to feed). The NC animals showed greater LMA (P=0.0001) and hot carcass weight (P=0.0006), and smaller BFT (P=0.0007), RFT (P=0.0039) and percentage of kidney, pelvic and heart fat (P<0.0001) when compared with IC animals. The animals fed ZH showed greater ADG (P=0.0002), G : F (P<0.0001) and dressing per cent (P=0.0136) than those fed RH and CON diets. No differences in BW and DMI were observed. A interaction between treatment and time on feed was observed for LMA and BFT, in which the animals fed ZH diet showed greater LMA (P<0.01) and lower BFT (P<0.01) at 100 days than the animals fed RH and CON diets, whereas RH and CON diets did not differ. Immunocastration decreases muscle development and increases carcass finishing. In contrast, ß-AA increases muscle and decreases fat deposition. The ZH has a higher action on the muscle metabolism than animals fed RH diet. However, RH diet achieves a better balance because it has an intermediary performance between non-supplemented and ZH animals and does not decrease the carcass fat.

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The aim of this study was to identify candidate genes and genomic regions associated with ultrasound-derived measurements of the rib-eye area (REA), backfat thickness (BFT) and rumpfat thickness (RFT) in Nellore cattle. Data from 640 Nellore steers and young bulls with genotypes for 290 863 single nucleotide polymorphisms (SNPs) were used for genomewide association mapping. Significant SNP associations were explored to find possible candidate genes related to physiological processes. Several of the significant markers detected were mapped onto functional candidate genes including ARFGAP3, CLSTN2 and DPYD for REA; OSBPL3 and SUDS3 for BFT; and RARRES1 and VEPH1 for RFT. The physiological pathway related to lipid metabolism (CLSTN2, OSBPL3, RARRES1 and VEPH1) was identified. The significant markers within previously reported QTLs reinforce the importance of the genomic regions, and the other loci offer candidate genes that have not been related to carcass traits in previous investigations.

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The genome-wide association study (GWAS) results are presented for average daily gain (ADG) in Nellore cattle. Phenotype of 720 male Bos indicus animals with information of ADG in feedlots and 354,147 single-nucleotide polymorphisms (SNPs) obtained from a database added by information from Illumina Bovine HD (777,962 SNPs) and Illumina BovineSNP50 (54,609) by imputation were used. After quality control and imputation, 290,620 SNPs remained in the association analysis, using R package Genome-wide Rapid Association using Mixed Model and Regression method GRAMMAR-Gamma. A genomic region with six significant SNPs, at Bonferroni-corrected significance, was found on chromosome 3. The most significant SNP (rs42518459, BTA3: 85849977, p = 9.49 × 10(-8)) explained 5.62% of the phenotypic variance and had the allele substitution effect of -0.269 kg/day. Important genes such as PDE4B, LEPR, CYP2J2 and FGGY are located near this region, which is overlapped by 12 quantitative trait locus (QTLs) described for several production traits. Other regions with markers with suggestive effects were identified in BTA6 and BTA10. This study showed regions with major effects on ADG in Bos indicus in feedlots. This information may be useful to increase the efficiency of selecting this trait and to understand the physiological processes involved in its regulation.

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We looked for possible associations of SNPs in genes related to protein turnover, with growth, feed efficiency and carcass traits in feedlot Nellore cattle. Purebred Nellore bulls and steers (N = 290; 378 ± 42 kg body weight, 23 months ± 42 days old) were evaluated for daily feed intake, body weight gain (BWG), gross feed efficiency, feed conversion ratio, partial efficiency of growth, residual feed intake (RFI), ultrasound backfat, rump fat, and ribeye area. Genotypes were obtained for SNPs in the growth hormone receptor (GHR-1 and GHR-2); calpain (CAPN4751); calpastatin (UoGCAST); ubiquitin-conjugating enzyme 2I (UBE2I-1 and UBE2I-2); R3H domain containing 1 (R3HDM1-1, -2, -3, and -4), ring finger protein 19 (RNF19); proteasome 26S subunit, non-ATPase, 13 (PSMD13); ribosomal protein, large, P2 (RPLP2); and isoleucine-tRNA synthetase 2, mitochondrial (IARS2) genes. Allelic substitution, additive and dominant effects were tested and molecular breeding values were computed. CAPN4751, GHR-1 and -2, IARS2, R3HDM1-4, and UoGCAST were found to be normally segregating polymorphisms. Additive and dominance effects were observed on BWG, feed efficiency and carcass traits, although dominant effects predominated. Significant allelic substitution effects were observed for CAPN4751, GHR-1 and -2, and UoGCAST on BWG, gross feed efficiency, RFI, and carcass traits, under single- or multiple-marker analyses. Correlations between molecular breeding values and phenotypes were low, excepted for RFI, based on allelic substitution estimates obtained by stepwise linear regression. We conclude that SNPs in genes related to protein turnover are related to economically important traits in Nellore cattle.

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Com o objetivo de determinar as exigências de energia e proteína para ganho de tourinhos Santa Gertrudes, 33 tourinhos, com idade de 12 meses e peso inicial médio de 314,6±33,2kg, foram confinados durante 115 dias, após 56 dias de adaptação. Seis animais foram abatidos após adaptação, para determinação da composição química corporal inicial. Os animais receberam dietas contendo 80% de concentrado, avaliando-se a inclusão de 0; 4,5; e 9,0% do subproduto concentrado da produção de lisina na matéria seca. As exigências de energia líquida de ganho (ELg) foram estimadas em função do peso de corpo vazio (PCVZ) e do ganho de PCVZ (GPCVZ), e as exigências líquidas de proteína para ganho (PLg) foram estimadas em função do GPCVZ e da energia retida (ER). As equações obtidas para ELg e PLg foram: ELg (Mcal/dia) = 0,0061×PCVZ0,75×GPCVZ0,578; e PLg (g/dia) = 208,1×GPCVZ - 1,0868×ER. A exigência de energia líquida encontrada para ganho de 1kg de PV foi de 3,93; 4,88 e 5,76Mcal, e a exigência de proteína metabolizável foi de 367,81; 393,59 e 391,63g, respectivamente, para animais com 300, 400 e 500kg de peso corporal. O valor de exigência líquida para mantença foi obtido por meio da regressão do logaritmo da produção de calor (PC), em função da ingestão de energia metabolizável, chegando-se ao valor de 75,6kcal/PVz0,75/dia. Concluiu-se que, devido ao aumento do teor de gordura na composição do ganho, animais de maior peso de corpo vazio apresentam maiores exigências líquidas de energia.

The objective of this study was to determine the energy and protein requirements for gain of Santa Gertrudis young bulls. Thirty-three 12-month-year-old animals, with initial body weight of 314.6±33.2kg, were kept in individual pens during 115 days, after 56 days of adaptation. Six animals were slaughtered after adaptation and determined the chemical composition of initial body. The concentrate proportion in the diet was 80%, on dry matter basis, and the levels of LPB studied were: 0, 4.5 or 9.0%. It was verified that the net energy requirement observed for weight gain of 1kg was 3.93, 4.88 and 5.76Mcal, and the metabolizable protein requirement was 367.81, 393.59 and 391.63g, respectively, for animals with 300, 400 and 500kg of body weight. The estimated maintenance energy requirement observed was 75.6kcal/LW0.75/day. The conclusion was thatbecause of the fat content in the gain composition, animalswith higher EBW have higher net energy requirements.

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Com o objetivo de determinar as exigências de energia e proteína para ganho de tourinhos Santa Gertrudes, 33 tourinhos, com idade de 12 meses e peso inicial médio de 314,6±33,2kg, foram confinados durante 115 dias, após 56 dias de adaptação. Seis animais foram abatidos após adaptação, para determinação da composição química corporal inicial. Os animais receberam dietas contendo 80% de concentrado, avaliando-se a inclusão de 0; 4,5; e 9,0% do subproduto concentrado da produção de lisina na matéria seca. As exigências de energia líquida de ganho (ELg) foram estimadas em função do peso de corpo vazio (PCVZ) e do ganho de PCVZ (GPCVZ), e as exigências líquidas de proteína para ganho (PLg) foram estimadas em função do GPCVZ e da energia retida (ER). As equações obtidas para ELg e PLg foram: ELg (Mcal/dia) = 0,0061×PCVZ0,75×GPCVZ0,578; e PLg (g/dia) = 208,1×GPCVZ - 1,0868×ER. A exigência de energia líquida encontrada para ganho de 1kg de PV foi de 3,93; 4,88 e 5,76Mcal, e a exigência de proteína metabolizável foi de 367,81; 393,59 e 391,63g, respectivamente, para animais com 300, 400 e 500kg de peso corporal. O valor de exigência líquida para mantença foi obtido por meio da regressão do logaritmo da produção de calor (PC), em função da ingestão de energia metabolizável, chegando-se ao valor de 75,6kcal/PVz0,75/dia. Concluiu-se que, devido ao aumento do teor de gordura na composição do ganho, animais de maior peso de corpo vazio apresentam maiores exigências líquidas de energia.(AU)

The objective of this study was to determine the energy and protein requirements for gain of Santa Gertrudis young bulls. Thirty-three 12-month-year-old animals, with initial body weight of 314.6±33.2kg, were kept in individual pens during 115 days, after 56 days of adaptation. Six animals were slaughtered after adaptation and determined the chemical composition of initial body. The concentrate proportion in the diet was 80%, on dry matter basis, and the levels of LPB studied were: 0, 4.5 or 9.0%. It was verified that the net energy requirement observed for weight gain of 1kg was 3.93, 4.88 and 5.76Mcal, and the metabolizable protein requirement was 367.81, 393.59 and 391.63g, respectively, for animals with 300, 400 and 500kg of body weight. The estimated maintenance energy requirement observed was 75.6kcal/LW0.75/day. The conclusion was thatbecause of the fat content in the gain composition, animalswith higher EBW have higher net energy requirements.(AU)

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RESUMO

To evaluate the effects of the supplementation of feed additives on carcass quality in beef cattle, 72 Nellore steers (339.5kg, 20-month old) were feedlot finished and fed for 91 days one of the following diets: 1) control with no additives; or added of 2) live yeast culture; 3) monensin; or 4) the association of both additives. After slaughter, renal, pelvic, and inguinal fat and hot carcass weights were recorded and carcass was split into muscle, bone, and trimmable fat. Carcass Longissimus muscle area and subcutaneous fat thickness at the 12th rib were measured and steaks of Longisimus muscle were taken to determine meat color, shear force, drip, and cooking losses. Yeast increased carcass dressing percentage but there were no effects on hot carcass weight, Longissimus area, subcutaneous fat thickness, percentage and weight of retail cut yield and trimmings. Feed additives had no effect on carcass pH, meat color, fat content, shear force, and drip losses. Supplementation of yeast, monensin or the association of both additives had no important effects on carcass traits and on meat quality of feedlot finished steers.

Avaliaram-se os efeitos da suplementação de aditivos alimentares sobre a qualidade de carcaça em bovinos de corte. Usaram-se 72 novilhos Nelore com média de peso de 339,5kg e 20 meses de idade, terminados em confinamento e alimentados por 91 dias com uma das quatro dietas: 1) dieta controle sem aditivos, ou com a adição de 2) leveduras vivas, 3) monensina ou 4) associação entre ambos aditivos. Após o abate, os pesos da gordura renal, pélvica e inguinal e da carcaça foram medidos e a carcaça dividida em músculos, ossos e aparas. Foram mensurados a área de olho de lombo e a espessura de gordura subcutânea sobre o músculo Longissimus na região da 12ª costela e foram obtidos bifes para a determinação da cor, força de cisalhamento e perdas por cocção e cozimento da carne. A levedura aumentou o rendimento de carcaça, mas não houve efeito dos tratamentos sobre o peso de carcaça, porção comestível e aparas. Os aditivos não influenciaram o pH da carcaça, a cor, a gordura intramuscular, a força de cisalhamento e as perdas por exsudação da carne. A suplementação, com levedura e com monensina em associação ou separadamente, não teve efeito importante sobre a qualidade da carcaça em novilhos terminados em confinamento.

Assuntos

RESUMO

To evaluate the effects of the supplementation of feed additives on carcass quality in beef cattle, 72 Nellore steers (339.5kg, 20-month old) were feedlot finished and fed for 91 days one of the following diets: 1) control with no additives; or added of 2) live yeast culture; 3) monensin; or 4) the association of both additives. After slaughter, renal, pelvic, and inguinal fat and hot carcass weights were recorded and carcass was split into muscle, bone, and trimmable fat. Carcass Longissimus muscle area and subcutaneous fat thickness at the 12th rib were measured and steaks of Longisimus muscle were taken to determine meat color, shear force, drip, and cooking losses. Yeast increased carcass dressing percentage but there were no effects on hot carcass weight, Longissimus area, subcutaneous fat thickness, percentage and weight of retail cut yield and trimmings. Feed additives had no effect on carcass pH, meat color, fat content, shear force, and drip losses. Supplementation of yeast, monensin or the association of both additives had no important effects on carcass traits and on meat quality of feedlot finished steers.(AU)

Avaliaram-se os efeitos da suplementação de aditivos alimentares sobre a qualidade de carcaça em bovinos de corte. Usaram-se 72 novilhos Nelore com média de peso de 339,5kg e 20 meses de idade, terminados em confinamento e alimentados por 91 dias com uma das quatro dietas: 1) dieta controle sem aditivos, ou com a adição de 2) leveduras vivas, 3) monensina ou 4) associação entre ambos aditivos. Após o abate, os pesos da gordura renal, pélvica e inguinal e da carcaça foram medidos e a carcaça dividida em músculos, ossos e aparas. Foram mensurados a área de olho de lombo e a espessura de gordura subcutânea sobre o músculo Longissimus na região da 12ª costela e foram obtidos bifes para a determinação da cor, força de cisalhamento e perdas por cocção e cozimento da carne. A levedura aumentou o rendimento de carcaça, mas não houve efeito dos tratamentos sobre o peso de carcaça, porção comestível e aparas. Os aditivos não influenciaram o pH da carcaça, a cor, a gordura intramuscular, a força de cisalhamento e as perdas por exsudação da carne. A suplementação, com levedura e com monensina em associação ou separadamente, não teve efeito importante sobre a qualidade da carcaça em novilhos terminados em confinamento.(AU)

Assuntos

RESUMO

The objectives were to evaluate preweaning performance, body composition, and efficiency of calves representing straightbred Nellore (NL), F(1), and 3-breed-cross systems. Energy requirements, milk production, and efficiency of 39 cow-calf pairs were recorded from straightbred NL calves from NL cows (10), crossbred (Angus-sired) calves from NL cows (ANL: 9), and crossbred calves (CC; Canchim-sired: 5/8 Charolais, 3/8 Zebu) from ANL (10) and Simmental x NL (10) cows. Cows and their respective calves were individually fed from birth to weaning (17 to 190 d postpartum). At 38 d of age, corn silage (7.8% CP, 2.19 Mcal of ME/kg of DM) was available to calves ad libitum. Milk production at 42, 98, 126, and 180 d postpartum was recorded by weighing calves before and after suckling. The ratio between GE and ME of milk was considered 1:0.93. Calves were slaughtered at weaning and the 9th-, 10th-, and 11th-rib section was removed for body composition estimation. The ANL calves were lighter (P < 0.01) at birth than the CC calves; the NL calves were intermediate. At weaning, the CC calves were heavier (P = 0.04) than the NL and ANL calves (230 +/- 5.5 vs. 172 +/- 8.1 and 209 +/- 8.6 kg, respectively). The ANL calves had greater (371 +/- 27 Mcal; P = 0.01) silage intake than the NL (270 +/- 25 Mcal) and CC (279 +/- 17 Mcal) calves. Milk energy intake was greater for the CC calves (970 +/- 38 Mcal of ME; P = 0.005) than the NL (670 +/- 57 Mcal of ME) and ANL (743 +/- 61 Mcal of ME) calves. The ANL calves compensated for the reduced milk production of the NL cows, which supplied less of their energy requirement for growth by increased silage intake. Calves from crossbred cows received a greater proportion of their total energy intake from milk. Crossbred calves had greater (P < 0.03) retained energy (retained energy = weaning body energy - birth body energy) than the NL calves (388 +/- 23 for ANL, and 438 +/- 15 for CC vs. 312 +/- 22 Mcal for NL calves). Percentages of water (P = 0.74) and chemical fat (P = 0.51) were similar among groups (63.7 +/- 0.6 and 14.3 +/- 0.7% for ANL calves, 63.1 +/- 0.4 and 14.7 +/- 0.5% for CC calves, and 63.3 +/- 0.6 and 13.7 +/- 0.7% of empty BW for water and chemical fat, respectively, for NL calves). Energetic efficiency (kcal of retained energy/Mcal of ME intake) was similar (P = 0.52) among groups (358 +/- 22 for ANL calves, 355 +/- 14 for CC calves, and 327 +/- 22 for NL calves). The greater BW gains and the differences in empty body composition at weaning were not enough to compensate for the greater ME intake of crossbreds. In this study, the crossbreeding systems evaluated increased preweaning calf performance but did not affect gross or energetic calf efficiency.

Assuntos

RESUMO

Determinou-se a digestibilidade aparente, pela coleta total de fezes, de dietas contendo 20, 40, 60 ou 80 por cento de concentrado para novilhos Brangus e Nelore. Oito animais, quatro de cada raça, foram utilizados em delineamento quadrado latino 4x4, com cinco dias de adaptação e cinco dias de coleta de fezes, em cada período. A idade e o peso iniciais dos Brangus e Nelore foram 13 meses e 248kg e 30 meses e 403kg, respectivamente. Houve efeito do nível de concentrado (P<0,01) sobre os coeficientes de digestibilidade da matéria seca (MS), proteína bruta (PB), extrato etéreo, fibra bruta, fibra em detergente neutro, extrativo não-nitrogenado (ENN), matéria orgânica (MO) e nutrientes digestíveis totais (NDT). A dieta com 80 por cento de concentrado apresentou maior digestibilidade da MS, PB e NDT. A digestibilidade do ENN e da MO e a ingestão de MS foram diferentes entre raças (P<0,05); os Brangus tiveram maior ingestão de MS e NDT (P<0,01). Houve interação entre raças e níveis de concentrado quanto à ingestão de MS e NDT (P<0,01), com maior ingestão pelos Brangus, nos níveis mais altos de NDT. Os Nelore foram menos adaptados às dietas ricas em concentrado em relação aos Brangus.

The apparent digestibility of diets with 20, 40, 60 or 80 percent of concentrate fed Brangus and Nellore steers was determined through total fecal collection. Eight animals, four of each breed, were used in two 4 x 4 Latin square design with five days of adaptation to the diets and five days of collection in each period. The initial age and body weight of Brangus and Nellore steers were 13 months and 248 kg, and 30 months and 403kg, respectively. Concentrate levels affected (P<0.01) the digestibility coefficients of dry matter (DM), crude protein (CP), ether extract, crude fiber, neutral detergent fiber, nitrogen free extract (NFE), organic matter (OM) and total digestible nutrients (TDN). The diet with 80 percent of concentrate showed higher DM, CP and TDN digestibilities. The digestibility of NFE, OM and the dry matter intake were different according to the breeds (P<0.05). Brangus showed higher DM and TDN intake. The DM and TDN intake showed interaction (P<0.01) breed by concentrate level, with higher intake for the Brangus at higher TDN diets. The Nellore steers were less adapted to diets rich in concentrate than Brangus steers.

Assuntos

RESUMO

Determinou-se a digestibilidade aparente, pela coleta total de fezes, de dietas contendo 20, 40, 60 ou 80 por cento de concentrado para novilhos Brangus e Nelore. Oito animais, quatro de cada raça, foram utilizados em delineamento quadrado latino 4x4, com cinco dias de adaptação e cinco dias de coleta de fezes, em cada período. A idade e o peso iniciais dos Brangus e Nelore foram 13 meses e 248kg e 30 meses e 403kg, respectivamente. Houve efeito do nível de concentrado (P<0,01) sobre os coeficientes de digestibilidade da matéria seca (MS), proteína bruta (PB), extrato etéreo, fibra bruta, fibra em detergente neutro, extrativo não-nitrogenado (ENN), matéria orgânica (MO) e nutrientes digestíveis totais (NDT). A dieta com 80 por cento de concentrado apresentou maior digestibilidade da MS, PB e NDT. A digestibilidade do ENN e da MO e a ingestão de MS foram diferentes entre raças (P<0,05); os Brangus tiveram maior ingestão de MS e NDT (P<0,01). Houve interação entre raças e níveis de concentrado quanto à ingestão de MS e NDT (P<0,01), com maior ingestão pelos Brangus, nos níveis mais altos de NDT. Os Nelore foram menos adaptados às dietas ricas em concentrado em relação aos Brangus.(AU)

The apparent digestibility of diets with 20, 40, 60 or 80 percent of concentrate fed Brangus and Nellore steers was determined through total fecal collection. Eight animals, four of each breed, were used in two 4 x 4 Latin square design with five days of adaptation to the diets and five days of collection in each period. The initial age and body weight of Brangus and Nellore steers were 13 months and 248 kg, and 30 months and 403kg, respectively. Concentrate levels affected (P<0.01) the digestibility coefficients of dry matter (DM), crude protein (CP), ether extract, crude fiber, neutral detergent fiber, nitrogen free extract (NFE), organic matter (OM) and total digestible nutrients (TDN). The diet with 80 percent of concentrate showed higher DM, CP and TDN digestibilities. The digestibility of NFE, OM and the dry matter intake were different according to the breeds (P<0.05). Brangus showed higher DM and TDN intake. The DM and TDN intake showed interaction (P<0.01) breed by concentrate level, with higher intake for the Brangus at higher TDN diets. The Nellore steers were less adapted to diets rich in concentrate than Brangus steers.(AU)

Assuntos

RESUMO

Determination of body composition is very important in nutritional and growth regulation studies. However, determination of body composition by grinding and analyzing all tissues is unfeasible as an experimental routine. The objective of this study was to test methodologies for body composition estimation. Linear measurements and chemical composition of the 9-10-11th and 10th rib sections were used to estimate chemical composition of 31 Nellore (Zebu) intact males with an average 333.5 kg body weight (range of 180.5 to 496.0) and 16.1% empty body lipid (range of 10.6 to 22.1). Composition of ribs, carcass and empty body were obtained by quantitatively grinding, homogenizing and sampling all body tissues. The 9-10-11th composition was a good estimator of body composition with r² of 0.99; 0.98; 0.98 and 0.91 for estimates of kg water, lipid, protein, and ash; with low standard errors of the estimate. Results with the 10th rib cut were similar (r² of 0.98, 0.98, 0.97 and 0.88 for the same regressions). Data are in the range of published results, however coefficients of regression were statistically different from those published for Bos taurus populations. Rib cut composition is a good parameter for the estimation of chemical body composition, but specific equations must be used for Zebu animals.

A determinação da composição corporal é fundamental em estudos nutricionais e da regulação do crescimento. Entretanto, a determinação direta é impraticável como rotina experimental. O objetivo deste estudo foi testar metodologias para estimar a composição química corporal. A composição da secções da 9-10-11ª e da 10ª costelas foram empregadas para estimar a composição corporal de 31 tourinhos Nelore com média de 333,5 kg de peso (variação de 180,5-496,0) e 16,1% de lípideo no corpo vazio (variação de 10,6-22,1). A composição dos costelas, carcaça e corpo vazio foram obtidas através de moagem quantitativa de todos os tecidos. A composição da 9-10-11ª costelas foi um bom estimador da composição com r² de 0,99; 0,98; 0,98 e 0,91 para as estimativas de kg de água, lipídeo, proteína e cinzas com baixo desvio padrão da estimativa. Os resultados com a secção da 10ª costela foi similar (r² de 0,98, 0,98, 0,97 e 0,88 para as mesmas regressões). Dados são consistentes com resultados da literatura, entretanto os coeficientes lineares das regressões foram estatisticamente diferentes daqueles publicados para Bos taurus. A composição de secções das costelas é um bom parâmetro para estimar a composição corporal, mas equações específicas devem ser utilizadas para zebuínos.

RESUMO

Determination of body composition is very important in nutritional and growth regulation studies. However, determination of body composition by grinding and analyzing all tissues is unfeasible as an experimental routine. The objective of this study was to test methodologies for body composition estimation. Linear measurements and chemical composition of the 9-10-11th and 10th rib sections were used to estimate chemical composition of 31 Nellore (Zebu) intact males with an average 333.5 kg body weight (range of 180.5 to 496.0) and 16.1% empty body lipid (range of 10.6 to 22.1). Composition of ribs, carcass and empty body were obtained by quantitatively grinding, homogenizing and sampling all body tissues. The 9-10-11th composition was a good estimator of body composition with r² of 0.99; 0.98; 0.98 and 0.91 for estimates of kg water, lipid, protein, and ash; with low standard errors of the estimate. Results with the 10th rib cut were similar (r² of 0.98, 0.98, 0.97 and 0.88 for the same regressions). Data are in the range of published results, however coefficients of regression were statistically different from those published for Bos taurus populations. Rib cut composition is a good parameter for the estimation of chemical body composition, but specific equations must be used for Zebu animals.

A determinação da composição corporal é fundamental em estudos nutricionais e da regulação do crescimento. Entretanto, a determinação direta é impraticável como rotina experimental. O objetivo deste estudo foi testar metodologias para estimar a composição química corporal. A composição da secções da 9-10-11ª e da 10ª costelas foram empregadas para estimar a composição corporal de 31 tourinhos Nelore com média de 333,5 kg de peso (variação de 180,5-496,0) e 16,1% de lípideo no corpo vazio (variação de 10,6-22,1). A composição dos costelas, carcaça e corpo vazio foram obtidas através de moagem quantitativa de todos os tecidos. A composição da 9-10-11ª costelas foi um bom estimador da composição com r² de 0,99; 0,98; 0,98 e 0,91 para as estimativas de kg de água, lipídeo, proteína e cinzas com baixo desvio padrão da estimativa. Os resultados com a secção da 10ª costela foi similar (r² de 0,98, 0,98, 0,97 e 0,88 para as mesmas regressões). Dados são consistentes com resultados da literatura, entretanto os coeficientes lineares das regressões foram estatisticamente diferentes daqueles publicados para Bos taurus. A composição de secções das costelas é um bom parâmetro para estimar a composição corporal, mas equações específicas devem ser utilizadas para zebuínos.

Assuntos