RESUMO
AIM: To evaluate the heart rate (HR) and behaviour of children and teenagers with Cerebral Palsy (CP) when having a dental appointment. METHODS: A comparative cross-sectional study was carried out with 60 participants with CP, between 2 and 14 years old (study group-SG), and 60 normotypical individuals (CG). The sample was paired according to age, gender and socioeconomic status. Behaviour was evaluated during dental prophylaxis using the Frankl Scale, and HR was measured at five moments: before the appointment, when sitting in the dental chair, during the clinical examination, during prophylaxis and immediately after prophylaxis. Mann-Whitney, Friedman and Chi-square tests were applied at a significance level of 5%. RESULTS: SG presented significantly higher HR (p < 0.001) and more participants with uncooperative behaviour (p < 0.001) than CG in all observational periods. Furthermore, SG participants with uncooperative behaviour presented higher HR values than those in SG who were cooperative in all observational periods (p ≤ 0.002). CONCLUSIONS: Individuals with CP have a higher HR before and during the clinical session, and are frequently more uncooperative with the procedure than normotypical individuals.
Assuntos
Paralisia Cerebral , Adolescente , Agendamento de Consultas , Criança , Pré-Escolar , Estudos Transversais , HumanosRESUMO
In central Brazil, in the municipality of Faina (state of Goiás), the small and isolated village of Araras comprises a genetic cluster of xeroderma pigmentosum (XP) patients. The high level of consanguinity and the geographical isolation gave rise to a high frequency of XP patients. Recently, two founder events were identified affecting that community, with two independent mutations at the POLH gene, c.764 + 1 G > A (intron 6) and c.907 C > T; p.Arg303* (exon 8). These deleterious mutations lead to the xeroderma pigmentosum variant syndrome (XP-V). Previous reports identified both mutations in other countries: the intron 6 mutation in six patients (four families) from Northern Spain (Basque Country and Cantabria) and the exon 8 mutation in two patients from different families in Europe, one of them from Kosovo. In order to investigate the ancestry of the XP patients and the age for these mutations at Araras, we generated genotyping information for 22 XP-V patients from Brazil (16), Spain (6) and Kosovo (1). The local genomic ancestry and the shared haplotype segments among the patients showed that the intron 6 mutation at Araras is associated with an Iberian genetic legacy. All patients from Goiás, homozygotes for intron 6 mutation, share with the Spanish patients identical-by-descent (IBD) genomic segments comprising the mutation. The entrance date for the Iberian haplotype at the village was calculated to be approximately 200 years old. This result is in agreement with the historical arrival of Iberian individuals at the Goiás state (BR). Patients from Goiás and the three families from Spain share 1.8 cM (family 14), 1.7 cM (family 15), and a more significant segment of 4.7 cM within family 13. On the other hand, the patients carrying the exon 8 mutation do not share any specific genetic segment, indicating an old genetic distance between them or even no common ancestry.
Assuntos
DNA Polimerase Dirigida por DNA/genética , Haplótipos , Padrões de Herança , Mutação , Isolamento Reprodutivo , Xeroderma Pigmentoso/genética , Brasil/epidemiologia , Consanguinidade , Europa (Continente)/epidemiologia , Éxons , Feminino , Genética Populacional , Heterozigoto , Homozigoto , Migração Humana , Humanos , Íntrons , Masculino , Fenótipo , Xeroderma Pigmentoso/epidemiologia , Xeroderma Pigmentoso/patologiaRESUMO
As a contact sport, Brazilian jiu-jitsu requires the fighter to expose his/her stomatognathic system to the adversary, making him/her more susceptible to oral and maxillofacial injuries and disorders. The aim of the present study was to determine the prevalence of injuries and disorders of the stomatognathic system and associated factors among practitioners of Brazilian jiu-jitsu. A total of 179 athletes were interviewed and submitted to a physical examination. The majority was male, in the beginner category and had participated in competitions. Athletes with more experience had a higher frequency of orofacial injuries (PR = 1.77; 95% CI: 1.01-1.38), such as oral mucous lacerations and skin abrasions in the facial region, which mainly occurred during training sessions. A mouthguard is not mandatory for the sport and many athletes (both beginners and more experienced athletes) do not use one due to difficulty breathing with the device. A prefabricated (type II) mouthguard was the most common among the athletes who used this equipment, although it does not offer adequate protection. Athletes on more advanced levels wore mouthguards significantly more often (PR = 1.96; 95% CI: 1.11-2.45). In conclusion, more experienced jiu-jitsu athletes had a higher frequency of orofacial injuries, such as lacerations and abrasions, and are more likely to wear a mouthguard. However, longitudinal studies are needed in order to assess the possible causes and risks.
Assuntos
Atletas , Artes Marciais , Sistema Estomatognático/lesões , Adolescente , Adulto , Brasil , Feminino , Humanos , Masculino , Protetores Bucais , Análise Multivariada , Análise de Regressão , Adulto JovemRESUMO
BACKGROUND: Despite the global decline in the detection of leprosy cases, its incidence has remained unchanged in certain settings and requires the determination of the factors linked to its persistence. We examined the spatial and space-time distribution of leprosy and the influence of social vulnerability on the occurrence of the disease in an endemic area of Northeast Brazil. METHODS: We performed an ecological study of all leprosy cases reported by Sergipe state, Northeast Brazil from 2001 to 2015, to examine the association of the Social Vulnerability Index and the prevalence and persistence of leprosy among the State's municipalities. Socio-economic and leprosy surveillance information was collected from the Brazilian information systems, and a Bayesian empirical local model was used to identify fluctuations of the indicators. Spatial and space-time clusters were identified using scan spatial statistic tests and to measure the municipalities' relative risk of leprosy. RESULTS: Leprosy clusters and burden of disease had a strong statistical association with the municipalities' Social Vulnerability Index. Municipalities with a high social vulnerability had higher leprosy incidence, multibacillary leprosy and newly diagnosed cases with grade 2 disability than areas with low social vulnerability. CONCLUSION: Social vulnerability is strongly associated with leprosy transmission and maintenance of disease incidence. Leprosy control programmes should be targeted to the populations with high social vulnerability.
Assuntos
Hanseníase/epidemiologia , Adolescente , Adulto , Teorema de Bayes , Brasil/epidemiologia , Humanos , Fatores de Risco , Adulto JovemRESUMO
Na medicina natural pouco se sabe sobre a importância curativa da maior parte das espécies pertencentes à Bromeliaceae. Tal fato evidencia a necessidade da realização de investigações para a comprovação de possíveis potenciais fitoterápicos em espécies dessa família. Guzmania lingulata é caracterizada como sendo uma das espécies de bromélia mais comercializada e conhecida popularmente, a qual é amplamente propagada no Brasil. O presente trabalho objetivou a caracterização anatômica e a identificação de classes de metabólitos secundários presentes em G. lingulata, por meio de análises anatômicas, histoquímicas e químicas. As folhas foram fixadas em FAA 50 e transferidas para etanol 50%. Para o estudo anatômico, secções transversais da porção mediana das lâminas foliares foram obtidas à mão livre e submetidas ao processo de dupla coloração com safranina e azul de astra, sendo, logo após, montadas em gelatina glicerinada. Para a triagem das classes de metabólitos secundários foram coletadas 10 folhas frescas que passaram pelo processo de maceração em solução hidroalcoólica 70% por sete dias, à temperatura ambiente. O filtrado resultante da filtração a vácuo foi evaporado sob vácuo a temperatura de 50ºC até a remoção total do etanol, sendo obtido desta forma o extrato bruto hidroalcoólico a frio (EBHF). Os resultados das secções histológicas permitiram a caracterização anatômico-foliar baseada na qualidade das secções manuais obtidas. Os testes em relação às classes de metabólitos secundários revelaram a presença de antocianinas e taninos e ausência de flavonóides e saponinas. Esses resultados estimulam a realização de estudos mais aprofundados para identificar e quantificar os compostos dessas e de outras classes de metabólitos.
In natural medicine, little is known about the healing importance of most of the species belonging to the family Bromeliaceae. Such a fact points tothe need to perform investigations toprovethe possible phytotherapic potentials in species of the family. Guzmania lingulata is characterized as one of the most popular well-known and commercialized species of bromeliads, being widely spread in Brazil. This work aimed at the anatomic characterization and identification of classes of the secondary metabolites present in G. lingulata, by anatomic, histochemical and chemical analysis of the leaves. The leaves were fixed in FAA 50 and, afterwards, transferred to 50% ethanol. For the anatomic study, transverse sections of the middleproportion of the leafblades were obtained by hand and exposed to the process of double coloration with safranin and astra blue, being, right after that, mounted on glycerin jelly. For the sorting of the classes of secondary metabolites, 10 fresh leaves were collected and then put through the maceration process in 70% hydroalcoholic solution for 7 days, at room temperature. The resulting filtered solution from the vacuum filtration was evaporated under vacuum at 40ºC until the complete removal of the ethanol, obtaining, this way, the cold crude hydroalcoholic extract (EBHF). The results ofhistological sectionsallowed the anatomical characterization based onthe quality ofthe manual sectionsobtained. The testsin relationto the classes of secondary metabolitesrevealed the presence ofanthocyanins andtannins andabsence of flavonoids and saponins. These results encourage the completionoffurther studies to identify and quantify the compounds of these and other classes of metabolites.
Assuntos
Bromeliaceae/classificação , Taninos/farmacologia , Folhas de Planta/metabolismo , Desidratação , Antocianinas/farmacologiaRESUMO
O presente trabalho teve por objetivo determinar a capacidade antioxidante de Oeceoclades maculata. A atividade foi determinada de acordo com a capacidade do extrato hidroalcoólico das folhas reduzir o radical DPPH. Os resultados obtidos permitem concluir que os conteúdos de polifenóis e flavonas/flavonóis são maiores no extrato preparado a frio (EBHF) do que no extrato preparado a quente (EBHQ), sugerindo que o aquecimento usado na preparação do extrato altera esses componentes ativos. Portanto, para esta espécie deve ser realizado o método de extração a frio para conservar as substâncias que possuem atividade antioxidante.
This work aimed to determine the antioxidant capacity of Oeceoclades maculata. The antioxidant activity was determined in accordance with the ability of the hydroalcoholic extract of the leaves to reduce the DPPH radical. The results showed that the polyphenol and flavonoid contents are greater in the cold extract (EBHF) than in the hot extract (EBHQ), which suggests that the heating effect in the preparation of the extract modifies these active compounds. Therefore, for this species, the cold extraction method must be carried in order to conserve the substances with antioxidant activity.
Assuntos
Compostos Fitoquímicos/análise , Plantas Medicinais/metabolismo , Flavonoides/classificação , Oxidantes/metabolismo , Orchidaceae/classificação , Polifenóis/classificaçãoRESUMO
Avaliou-se o efeito da somatotropina bovina recombinante (rbST) sobre a qualidade do corpo lúteo e do embrião e sobre a concentração de progesterona (P4), em ovelhas da raça Santa Inês doadoras de embriões. Foram utilizadas 13 doadoras, distribuídas aleatoriamente em dois grupos, G1 (n=7) e G2 (n=6). Em ambos os grupos, as doadoras foram sincronizadas com implante vaginal por 14 dias, receberam 100µg de PGF2α (Ciosin®) no sétimo dia e foram superovuladas, a partir do 12º dia, com 250UI de FSH (Folltopin-V®) em seis doses decrescentes. No dia da retirada do implante, as doadoras do G2 receberam 125mg de rbST (Boostin®) e foram cobertas por macho Santa Inês a cada 12 horas até o final do estro. No sexto dia após a primeira cobrição, foram avaliadas, por laparoscopia, a resposta superovulatória e a quantidade e a qualidade dos corpos lúteos. Após as avaliações, as colheitas embrionárias foram realizadas por laparotomia. A administração da rbST no protocolo de superovulação promoveu aumento significativo (P<0,05) no número de corpos lúteos do tipo I (9,00 x 5,28) e na qualidade embrionária (4,33 x 2,00).(AU)
We evaluate the effect of recombinant bovine somatotropin (rbST) on the quality of the corpus luteum and embryo and on the concentration of progesterone (P4) in donors embryo sheep Santa Ines. 13 donors were used, randomly assigned to two groups, G1 (n=7) and G2 (n=6). In both groups, the donors were synchronized with vaginal implant for 14 days and received 100µg of PGF2α (Ciosin®) on the seventh day and were superovulated, from the 12th day, with 250IU of FSH (Folltopin-V®) in six decreasing doses. On the day of implant removal the donor G2 received 125mg of rbST (Boostin®), and were covered by Santa Inês male every 12 hours until the end of estrus. In the sixth day after the first mating were evaluated by laparoscopy, superovulatory response and the amount and quality of the corpus luteum. After the evaluations, the embryonic harvests were performed by laparotomy. The administration of rbST in superovulation protocol promoted a significant increase (P <0.05) in the number of corpus luteum of type I(9.00 x 5.28) and embryo quality (4.33 x 2.00).(AU)
Assuntos
Animais , Feminino , Corpo Lúteo/anatomia & histologia , Hormônio Foliculoestimulante/administração & dosagem , Estro , Oócitos/química , Oócitos/crescimento & desenvolvimento , Fertilização/fisiologiaRESUMO
Avaliou-se o efeito da somatotropina bovina recombinante (rbST) sobre a qualidade do corpo lúteo e do embrião e sobre a concentração de progesterona (P4), em ovelhas da raça Santa Inês doadoras de embriões. Foram utilizadas 13 doadoras, distribuídas aleatoriamente em dois grupos, G1 (n=7) e G2 (n=6). Em ambos os grupos, as doadoras foram sincronizadas com implante vaginal por 14 dias, receberam 100µg de PGF2α (Ciosin®) no sétimo dia e foram superovuladas, a partir do 12º dia, com 250UI de FSH (Folltopin-V®) em seis doses decrescentes. No dia da retirada do implante, as doadoras do G2 receberam 125mg de rbST (Boostin®) e foram cobertas por macho Santa Inês a cada 12 horas até o final do estro. No sexto dia após a primeira cobrição, foram avaliadas, por laparoscopia, a resposta superovulatória e a quantidade e a qualidade dos corpos lúteos. Após as avaliações, as colheitas embrionárias foram realizadas por laparotomia. A administração da rbST no protocolo de superovulação promoveu aumento significativo (P<0,05) no número de corpos lúteos do tipo I (9,00 x 5,28) e na qualidade embrionária (4,33 x 2,00).
We evaluate the effect of recombinant bovine somatotropin (rbST) on the quality of the corpus luteum and embryo and on the concentration of progesterone (P4) in donors embryo sheep Santa Ines. 13 donors were used, randomly assigned to two groups, G1 (n=7) and G2 (n=6). In both groups, the donors were synchronized with vaginal implant for 14 days and received 100µg of PGF2α (Ciosin®) on the seventh day and were superovulated, from the 12th day, with 250IU of FSH (Folltopin-V®) in six decreasing doses. On the day of implant removal the donor G2 received 125mg of rbST (Boostin®), and were covered by Santa Inês male every 12 hours until the end of estrus. In the sixth day after the first mating were evaluated by laparoscopy, superovulatory response and the amount and quality of the corpus luteum. After the evaluations, the embryonic harvests were performed by laparotomy. The administration of rbST in superovulation protocol promoted a significant increase (P <0.05) in the number of corpus luteum of type I(9.00 x 5.28) and embryo quality (4.33 x 2.00).
Assuntos
Animais , Feminino , Corpo Lúteo/anatomia & histologia , Hormônio Foliculoestimulante/administração & dosagem , Estro , Fertilização/fisiologia , Oócitos/crescimento & desenvolvimento , Oócitos/químicaRESUMO
This work aimed at investigating the lipid profile of zoonotic visceral leishmaniasis (VL) patients' sera and the effect of lipoproteins on the in vitro production of tumour necrosis factor-alpha (TNF-alpha), interleukin (IL)-6, IL-10 and IL-12 by Leishmania infantum-infected and uninfected macrophages. Lipids were quantified in 26 VL patients' sera and 26 healthy controls from a VL endemic area. The patients' sera had higher triglyceride and very low density lipoprotein (VLDL) levels, and much lower apolipoprotein A1, total cholesterol, low density lipoprotein (LDL) and high density lipoprotein (HDL) levels than the control sera. Lipoprotein fractions were obtained by ultracentrifugation of sera. The addition of LDL and HDL to Leishmania-infected and uninfected macrophages, in physiological concentrations, enhanced the production of IL-6 and IL-10, but not of IL-12. LDL stimulated the production of TNF-alpha only in infected macrophages, whereas HDL stimulated the production of lower amounts of TNF-alpha in both infected and uninfected macrophages. VLDL stimulated only the production of IL-10. It is proposed herein that LDL may influence the development of VL by promoting the production of TNF-alpha by infected macrophages. A decrease in plasma LDL in some VL patients (to 20 mg/mL or less); however, would tend to reduce the production of TNF-alpha and therefore to limit the development of immune-mediated pathology, not withstanding the fact that it would perhaps increase the permissiveness of macrophages to Leishmania growth.
Assuntos
Leishmaniose Visceral/imunologia , Leishmaniose Visceral/patologia , Lipídeos/sangue , Lipoproteínas/sangue , Macrófagos/imunologia , Macrófagos/parasitologia , Adulto , Animais , Criança , Pré-Escolar , Citocinas/metabolismo , Feminino , Humanos , Lactente , Lipoproteínas/isolamento & purificação , Masculino , Ultracentrifugação , Adulto JovemRESUMO
OBJECTIVE: Oral estrogens improve endothelial function, and for this reason may be considered cardioprotective; however, in women with coronary heart disease there may also be an increase in the risk of thrombosis. Although transdermal estrogen administration may decrease this adverse effect, there are few data on endothelial function in women with coronary heart disease treated using such therapy. This study aimed to report the endothelial response in postmenopausal women with coronary heart disease treated with transdermal estrogen. MATERIALS AND METHODS: This was a double-blind, prospective, randomized study. Eighteen patients with a history of acute coronary syndrome and nine healthy women were studied over 4 weeks. Coronary patients were assigned at random to receive a patch containing either 50 microg estradiol or placebo on a weekly basis. Endothelial function was assessed by flow-mediated vasodilatation of the brachial artery. Baseline blood flow (brachial artery diameter) was measured after 30 min rest and following ischemia, prior to treatment and after 4 weeks. RESULTS: Flow-mediated vasodilatation in normal patients was 17.8%, whereas in women with coronary disease it was 1.2% (p = 0.0001). Arterial diameter for the resting period in coronary disease subjects increased from 4.22 +/- 0.59 to 4.41 +/- 0.56 mm (p < 0.004) after 4 weeks of estrogen therapy, whereas, in women receiving placebo, it did not change. Flow-mediated vasodilatation in the estrogen group was 3.4% and in the placebo group was 0.5% (p = 0.05). CONCLUSIONS: Transdermal estrogen may improve endothelial function in women with coronary heart disease.
Assuntos
Braço/irrigação sanguínea , Artéria Braquial/fisiologia , Doença da Artéria Coronariana , Estradiol/farmacologia , Administração Cutânea , Velocidade do Fluxo Sanguíneo , Artéria Braquial/efeitos dos fármacos , Método Duplo-Cego , Endotélio Vascular/efeitos dos fármacos , Endotélio Vascular/fisiologia , Estradiol/administração & dosagem , Feminino , Humanos , Pessoa de Meia-Idade , Pós-Menopausa , Estudos Prospectivos , Resultado do Tratamento , Vasodilatação/efeitos dos fármacos , Vasodilatação/fisiologiaRESUMO
AIMS: To investigate genetic diversity among Staphylococcus aureus and to delineate the geographical distribution of the strains found. METHODS AND RESULTS: RAPD-PCR and ribotyping-PCR were employed for the characterization of Staph. aureus isolates from bovine and nosocomial origin. Among the strains, five to nine groups were distinguished by RAPD-PCR, depending on which primer was used, while ribotyping-PCR distinguished seven ribotypes. CONCLUSIONS, AND SIGNIFICANCE AND IMPACT OF THE STUDY: These results demonstrate the genetic heterogeneity of the strains studied, and the large dissemination of some clones throughout different regions and hosts, findings that may allow the monitoring of Staph. aureus infections.
Assuntos
Técnicas de Tipagem Bacteriana , Técnica de Amplificação ao Acaso de DNA Polimórfico , Ribotipagem , Staphylococcus aureus/genética , Animais , Bovinos , Impressões Digitais de DNA , Ecologia , Heterogeneidade Genética , Humanos , Reação em Cadeia da Polimerase , Estudos Soroepidemiológicos , Staphylococcus aureus/classificaçãoRESUMO
BACKGROUND: In Chile, 40% of deaths due to acute myocardial infarction occur in women. AIM: To assess the presence of cardiovascular risk factors in women with acute coronary syndromes. PATIENTS AND METHODS: Thirty four women aged 46 to 55 years old, admitted to the hospital due to an acute coronary syndrome and 102 age matched healthy women were studied. A clinical history was obtained, blood pressure and fasting serum lipids were measured. RESULTS: A history of high blood pressure was present in 65 and 16% of patients and healthy controls respectively. Seventy seven percent of patients and 36% of controls smoked, 76% of patients and 48% of controls were postmenopausal, 21% of patients an 5% of controls were diabetic. In patients and controls respectively serum total cholesterol was 230.1 +/- 36.2 and 211.2 +/- 34.8 mg/dl, serum triacylglycerol was 213.4 +/- 109.4 and 143.2 +/- 76.9 mg/dl and serum HDL cholesterol was 44.1 +/- 10.8 and 49.8 +/- 13.3 mg/dl (p < 0.001 or less). In univariate analysis, the risk of acute coronary syndrome increased with high blood pressure (OR: 9.3, CI: 2.5-18.6), menopause (OR: 8.3, CI: 2.2-31:5), smoking (OR: 6.9, CI: 2.5-18.6), diabetes mellitus (OR: 5.0, CI: 1.4-17.5), a high total cholesterol/HDL cholesterol ratio (OR: 6.6, CI: 1.8-12.5) and hypertriglyceridemia (OR: 3.6, CI: 1.5-8.5). Logistic regression analysis showed that hypertension and menopause had the higher predictive values for acute coronary syndrome. CONCLUSIONS: In this group of women with acute coronary syndromes, the main coronary risk factors were high blood pressure and menopause.
Assuntos
Doença das Coronárias/etiologia , Análise de Variância , Estudos de Casos e Controles , Chile/epidemiologia , HDL-Colesterol/sangue , Doença das Coronárias/sangue , Doença das Coronárias/epidemiologia , Feminino , Humanos , Hipertensão/complicações , Menopausa/sangue , Pessoa de Meia-Idade , Prevalência , Fatores de Risco , SíndromeRESUMO
In order to improve information about the microbiological quality of the milk commercially available in the city of Recife, 250 samples of pasteurized type-C milk and 50 samples of raw milk were analyzed for Yersinia enterocolitica and Listeria monocytogenes and verify the possible occurrence of Yersinia enterocolitica and Listeria monocytogenes. These bacteria can develop in refrigeration temperatures and are responsible for food-born diseases. Neither Y. enterocolitica nor L. monocytogenes were found in the samples analyzed. However, the presence of Y. intermedia and Y. frederiksenii was detected, these environmental species behave as opportunist pathogens. Through the methodology used for Listeria isolation, one isolate of Salmonella Montevideo was obtained from a sample of pasteurized milk and another isolated from one sample of raw milk. Besides these, several other bacteria species were found. It is likely that the large microbiota present in the samples and the procedures employed to destroy it could have hindered the isolation of Y. enterocolitica and L. monocytogenes.
Assuntos
Listeria monocytogenes/isolamento & purificação , Leite/microbiologia , Salmonella/isolamento & purificação , Yersinia enterocolitica/isolamento & purificação , Animais , Brasil , Manipulação de AlimentosRESUMO
In order to improve information about the microbiological quality of the milk commercially available in the city of Recife, 250 samples of pasteurized type-C milk and 50 samples of raw milk were analyzed for Yersinia enterocolitica and Listeria monocytogenes and verify the possible occurrence of Yersinia enterocolitica and Listeria monocytogenes. These bacteria can develop in refrigeration temperatures and are responsible for food-born diseases. Neither Y. enterocolitica nor L. monocytogenes were found in the samples analyzed. However, the presence of Y. intermedia and Y. frederiksenii was detected, these environmental species behave as opportunist pathogens. Through the methodology used for Listeria isolation, one isolate of Salmonella Montevideo was obtained from a sample of pasteurized milk and another isolated from one sample of raw milk. Besides these, several other bacteria species were found. It is likely that the large microbiota present in the samples and the procedures employed to destroy it could have hindered the isolation of Y. enterocolitica and L. monocytogenes.
Visando complementar as informações sobre a qualidade microbiológica do leite comercializado na cidade do Recife, foram analisadas 250 amostras de leite pasteurizado tipo C e 50 amostras de leite cru para a pesquisa de Yersinia enterocolitica e Listeria monocytogenes, bactérias patogênicas capazes de se desenvolverem em temperatura de refrigeração. Y. enterocolitica não foi encontrada em nenhuma das amostras analisadas, entretanto foi detectada a presença de Y. intermedia e Y. frederiksenii, espécies ambientais que se comportam como patógenos oportunistas. L. monocytogenes também não foi encontrada, mas, através da metodologia empregada para seu isolamento foi obtido um isolamento de Salmonella Montevideo em uma amostra de leite pasteurizado e outro em leite cru. Além dessas, várias outras bactérias foram encontradas, supondo-se que a ampla microbiota crescida nos meios empregados pode ter interferido no isolamento da Y. enterocolitica e L. monocytogenes.
Assuntos
Animais , Leite/microbiologia , Listeria monocytogenes/isolamento & purificação , Salmonella/isolamento & purificação , Yersinia enterocolitica/isolamento & purificação , Brasil , Manipulação de AlimentosRESUMO
En este trabajo se presenta la evaluación a nivel laboratorio de la eficaciA de la desinfección de muestras de barro crudo artificialmente contaminado con cepa epidémica de Vibrio cholerae biotipo El Tor. La investigación se realizó con muestras de la Planta depuradora cloacal de Belem(Curitiba)
Assuntos
Brasil , Tratamento de Lodos , Remoção de Contaminantes , Vibrio choleraeRESUMO
Através das análises efetuadas, em 96 amostras de hortaliças cruas, coletadas em 5 restaurantes da cidade do Recife, que servem almoço no peso, não foram encontradas Yersinia enterocolitica nem outras enterobactérias patogênicas. As análises realizadas a partir dos "swabs" orais e retais, obtidos em 15 suínos aparentemente sadios do município de Bonito, no Estado de Pernambuco, também não evidenciaram a presença de Y. enterocolitica. Foram obtidas amostras para análises em 22 roedores e um espécimen de marsupial, entre os quais também não foram encontrados nem Y. enterocolitica nem outros enteropatógenos.
A search for the presence of enteropathogenic bacteria in fresh vegetables obtained in 5 restaurants from the city of Recife, revealed neither Yersinia enterocolitica nor other pathogenic bacteria in 96 samples analyzed. Furthermore, Y. enterocolitica was not found in the oral and rectal swabs taken from 15 apparently healthy pigs at an abattoir in the municipality of Bonito in the Pernambuco State. Another search in which twenty one rodents from four species and one marsupial specimen were examined did not detect the presence of Yersinia and other enteropathogenic bacteria.
Assuntos
Animais , Microbiologia de Alimentos , Reservatórios de Doenças/veterinária , Yersinia enterocolitica/isolamento & purificação , Matadouros , Brasil , Mamíferos/microbiologia , Restaurantes , Salmonella/isolamento & purificação , Suínos/microbiologiaRESUMO
A search for the presence of enteropathogenic bacteria in fresh vegetables obtained in 5 restaurants from the city of Recife, revealed neither Yersinia enterocolitica nor other pathogenic bacteria in 96 samples analyzed. Furthermore, Y. enterocolitica was not found in the oral and rectal swabs taken from 15 apparently healthy pigs at an abattoir in the municipality of Bonito in the Pernambuco State. Another search in which twenty one rodents from four species and one marsupial specimen were examined did not detect the presence of Yersinia and other enteropathogenic bacteria.
Assuntos
Reservatórios de Doenças/veterinária , Microbiologia de Alimentos , Yersinia enterocolitica/isolamento & purificação , Matadouros , Animais , Brasil , Mamíferos/microbiologia , Restaurantes , Salmonella/isolamento & purificação , Suínos/microbiologiaRESUMO
En este trabajo se presenta la evaluación a nivel laboratorio de la eficaciA de la desinfección de muestras de barro crudo artificialmente contaminado con cepa epidémica de Vibrio cholerae biotipo El Tor. La investigación se realizó con muestras de la Planta depuradora cloacal de Belem(Curitiba)
Assuntos
Brasil , Remoção de Contaminantes , Tratamento de Lodos , Vibrio choleraeRESUMO
Body weight loss and growth retardation occur in rats exposed to simulated high altitude, which may be related to the hypoxemia-induced reduction in the convective oxygen transport (COT). The present study was thus performed to determine whether transfusion polycythemia, increased affinity of hemoglobin for oxygen, or previous acclimation to hypobaria (factors that increase COT) are able to counteract its effect on body weight during the early period of exposure, which appears to be a suitable parameter to test the effectiveness of acclimatization. Polycythemia was induced in weanling rats by two ip injections of 2.5 ml/100 g b.wt of packed homologous red cells. The rise in hemoglobin O2 affinity was brought about in adult rats by giving them 0.5 g/dl sodium cyanate in the drinking water for 3 weeks. A lower body weight loss during the early period of exposure to hypobaria was seen in treated rats than in controls. However, body weight loss was still important, which would indicate that compensation was probably not complete. When growing rats were acclimated to simulated altitude, a sudden increase in body weight was observed when they were brought back to ground levels. When animals were taken to altitude again, they lost weight at a rate not significantly different to that found in non-acclimated ones. The results obtained indicate that treatments do not prevent the studied effect of hypoxia and suggest that hypophagia and the resultant initial body weight loss and secondary depression of body growth could be considered as protective mechanisms against the environmental challenge, although further investigation will be necessary to confirm the hypothesis.