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Rheumatoid arthritis (RA) and osteoarthritis (OA) are the most common arthritic diseases. Medical ozone has demonstrated its effectiveness in combination therapy with methotrexate or non-steroidal anti-inflammatory drugs for RA and OA, respectively. Although RA and OA have been compared from different points of view, few studies have considered their redox status in spite of the oxidative processes that are involved in both diseases. The aim of this study was to compare RA with OA, evaluating their redox status and the effects of ozone on their clinical response to combined therapy with ozone. The redox status of 80 patients was determined: antioxidant defenses, injury markers, two subjective variables (pain and disability), and levels of antibodies against cyclic citrullinated peptides were evaluated. Oxidative stress and clinical response to combined therapy with ozone was higher than in the case of RA. After medical ozone treatment, there was an increase in antioxidant defense and a decrease in injury markers as well as pain, disability, and autoantibody concentrations. Redox biomarkers were able to differentiate between both arthritic diseases and combined therapy with ozone (methotrexate + ozone), showing a therapeutic selectivity for RA in comparison with OA.
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Medical ozone reestablishes cellular redox balance so that it may be a valid therapeutic approach in the prevention and management of age-related diseases with oxidative etiology in older people. The aim of this study is to evaluate oxidative stress and some vasoactive substances in elderly (60-70 years) rheumatoid arthritis patients with diabetes and hypertension, as well as another group with bronchial asthma patients in order to demonstrate the beneficial effects of medical ozone in the prevention and therapy of age-related diseases in these age groups. A randomized clinical study with 45 older patients (60-70 years) was performed. Group I (n = 15) with rheumatoid arthritis + diabetes and hypertension received no ozone treatment, and group II (n = 30) was treated with medical ozone. This group was divided into two subgroups (n = 15 each), group IIa: the same as group I + medical ozone and group IIb: bronchial asthma patients. Indicators of RA in I and IIa groups were evaluated. Redox balance was assessed through defense and injury biomarkers. Thromboxane A2 (TXA2) and prostacyclin levels were assessed in group IIb patients. Medical ozone arrested oxidative injury progression in the Ia group and decreased thromboxane levels and the TXA2/6-keto PGF1α ratio in the IIb group. Medical ozone arrested the progression of oxidative damage and modulated those endogenous mechanisms that promote a suitable redox status and TXA2/PGI2 balance. These results suggest that medical ozone may become a standard approach in the prevention and management of age-related oxidative diseases in elderly people.
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Introducción: El ozono médico tiene eficacia clínica e incrementa la relación beneficio/riesgo en pacientes con artritis reumatoide tratados con la terapia combinada metotrexate + ozono. Hoy, la gamma glutamil transferasa se considera como un marcador de riesgo de enfermedades de una alta morbilidad y mortalidad, y tiene particular valor en la artritis reumatoide por desempeñar un papel patológico asociado al estrés oxidativo y a la remodelación ósea, lo que causa daño al cartílago y al hueso. Objetivo: Evaluar los efectos del ozono médico sobre los niveles de gamma glutamil transferasa. Métodos: Se estudiaron pacientes portadores de dos enfermedades artríticas: artritis reumatoide (n = 100; grupo tratado con metotrexate [n = 50] y grupo con metotrexate + ozono [n = 50]) y osteoartritis de rodilla (n = 40; grupo precondicionado con ozono antes de la artroscopía [n = 20] y grupo sin pretratamiento con ozono antes de la artroscopía [n = 20]). Los pacientes con artritis reumatoide fueron valorados con indicadores clínicos específicos, incluidos los niveles de anticuerpos contra péptidos cíclicos citrulinados, así como las concentraciones de glutatión reducido, importante antioxidante endógeno. Resultados: El ozono médico reguló la actividad sérica de gamma glutamil transferasa. Correlacionó de forma inversamente proporcional con los niveles de glutatión reducido que, a su vez, fue el único marcador redox que para los pacientes tratados con la terapia combinada metotrexate + ozono fue directamente proporcional con todas las variables clínicas evaluadas. Conclusión: Se debe considerar a la gamma glutamil transferasa un indicador de la eficacia clínica del ozono médico en las enfermedades estudiadas, por su doble función: biomarcador de estrés oxidativo e indicador de la remodelación patológica del hueso(AU)
Introduction: Medical ozone has demonstrated its clinical efficacy as well as the increase of beneficial/risk relationship in rheumatoid arthritis patients treated with metotrexate+ozone combined therapy. At present, gamma-glutamyl transpeptidase is considered as risk indicator of high morbimortality diseases. It has a special value in arthritis diseases due to its pathologic role associated to oxidative stress and in the abnormal bone remodeling processes. Objective: Assess the ozone medical effects on gamma-glutamyl transpeptidase levels. Method: Patients who suffered of two arthritic diseases: rheumatoid arthritis (n=100; Group treated with Metotrexate (n=50) and metotrexate+ozone (n=50) and knee osteoarthritis (n=40); Group preconditioned with ozone before arthroscopy (n=20) and Group without previous treatment with ozone before arthroscopy (n=20). Rheumatoid arthritis patients were assessed through specific clinic indicators which included antibodies against cyclic citrullinate peptides as well as reduced gluthatione concentrations which are an important endogenous antioxidant. Results: Medical ozone regulated serum gamma-glutamyl transpeptidase activity which correlated in inverse proportion to reduced glutathione levels which was the only one redox marker that correlated with all clinical variables (p < 0.05) when patients were treated with metotrexate+ozone. Conclusion: Gamma-glutamyl transpeptidase should be considered as biomarker of medical ozone clinical efficacy in rheumatoid arthritis and knee osteoarthritis due to GGT´s both pathologic functions: indicator of oxidative stress and abnormal bone remodeling processes(AU)
Assuntos
Humanos , Masculino , Feminino , Ozônio/uso terapêutico , Artrite Reumatoide/terapia , Osteoartrite do Joelho , gama-Glutamiltransferase/análise , Resultado do Tratamento , Terapia CombinadaRESUMO
INTRODUCTION/OBJECTIVES: Although inflammation in disc hernia (DH) has been recognized and it is a well-known process mediated by loss of the cellular redox balance, only a few studies about the impact of chronic oxidative stress on this neurological disorder have been made. Ozone therapy has been widely used with clinical efficacy in DH. This work aimed at characterizing the systemic redox status of patients with low back pain and neck pain as well as studying if ozone oxidative post-conditioning modified the pathological oxidative stress and protected against oxidative protein damage and if there is any relationship between oxidative changes and pain in both DH. METHODS: Redox status of 33 patients with diagnosis of DH by computerized axial tomography, nuclear magnetic resonance, and clinical evaluations was studied. Ozone was administered by paravertebral way. After ozone treatment, plasmatic levels of antioxidant/pro-oxidant markers, pain, and life quality disability parameters were evaluated. RESULTS: One hundred percent of patients showed a severe oxidative stress. Major changes in superoxide dismutase activity, total hydroperoxides, advanced oxidation protein products, fructolysine content, and malondialdehyde were observed. After ozone oxidative post-conditioning, there was a re-establishment of patients' cellular redox balance as well as a decrease in pain in both DH. A relationship between indicators of oxidative protein damage and pain was demonstrated. CONCLUSIONS: Ozone therapy protected against oxidation of proteins and reduced the pain. Relationship between markers of oxidative protein damage, disability parameters, and pain suggests the role of oxidative stress in the pathological processes involved in DH.
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Deslocamento do Disco Intervertebral/tratamento farmacológico , Estresse Oxidativo/efeitos dos fármacos , Ozônio/uso terapêutico , Adulto , Biomarcadores/metabolismo , Feminino , Humanos , Deslocamento do Disco Intervertebral/metabolismo , Masculino , Pessoa de Meia-Idade , Oxirredução/efeitos dos fármacos , Ozônio/administração & dosagem , Ozônio/farmacologia , Proteínas/metabolismo , Adulto JovemRESUMO
Proteins are major target for radicals and other oxidants when these are formed in both intra- and extracellular environments in vivo. Formation of lesions on proteins may be highly sensitive protein-based biomarkers for oxidative damage in mammalian systems. Oxidized proteins are often functionally inactive and their unfolding is associated with enhanced susceptibility to proteinases. ROS scavenging activities of intact proteins are weaker than those of misfolded proteins or equivalent concentrations of their constituent amino acids. Protein oxidation and enhanced proteolytic degradation, therefore, have been suggested to cause a net increase in ROS scavenging capacity. However, certain oxidized proteins are poorly handled by cells, and together with possible alterations in the rate of production of oxidized proteins, may contribute to the observed accumulation and damaging actions of oxidized proteins during ageing and in pathologies such as diabetes, arteriosclerosis and neurodegenerative diseases. Protein oxidation may play a controlling role in cellular remodelling and cell growth. There is some evidence that antioxidant supplementation may protect against protein oxidation, but additional controlled studies of antioxidant intake to evaluate the significance of dietary/pharmacological antioxidants in preventing physiological/pathological oxidative changes are needed.
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Estresse Oxidativo , Animais , Antioxidantes/farmacologia , Radicais Livres , Homeostase , Humanos , Modelos Biológicos , Doenças Neurodegenerativas/patologia , Oxigênio/química , Oxigênio/metabolismo , Proteínas/química , Espécies Reativas de OxigênioRESUMO
The effect of Mangifera indica L. extract (Vimang) on treatment of injury associated with hepatic ischaemia/reperfusion was tested. Vimang protects from the oxidative damage induced by oxygen-based free radicals as shown in several in vitro test systems conducted. The ability of Vimang to reduce liver damage was investigated in rats undergoing right-lobe blood fl ow occlusion for 45 min followed by 45 min of reperfusion. The ischaemia/reperfusion model leads to an increase of transaminase (ALT and AST), membrane lipid peroxidation, tissue neutrophil in filtration, DNA fragmentation, loss of protein -SH groups, cytosolic Ca2+ overload and a decrease of catalase activity. Oral administration of Vimang (50, 110 and 250 mg/kg, b.w.) 7 days before reperfusion, reduced transaminase levels and DNA fragmentation in a dose dependent manner (p < 0.05). Vimang also restored the cytosolic Ca2+ levels and inhibited polymorphonuclear migration at a dose of 250 mg/kg b.w., improved the oxidation of total and non protein sulfhydryl groups and prevented modification in catalase activity, uric acid and lipid peroxidation markers (p < 0.05). These data suggest that Vimang could be a useful new natural drug for preventing oxidative damage during hepatic injury associated with free radical generation.
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Antioxidantes/uso terapêutico , Isquemia/tratamento farmacológico , Fígado/irrigação sanguínea , Mangifera , Fitoterapia , Extratos Vegetais/uso terapêutico , Traumatismo por Reperfusão/tratamento farmacológico , Administração Oral , Alanina Transaminase/sangue , Alanina Transaminase/efeitos dos fármacos , Animais , Antioxidantes/administração & dosagem , Antioxidantes/farmacologia , Aspartato Aminotransferases/sangue , Aspartato Aminotransferases/efeitos dos fármacos , Feminino , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/enzimologia , Testes de Função Hepática , Casca de Planta , Extratos Vegetais/administração & dosagem , Extratos Vegetais/farmacologia , Ratos , Ratos WistarRESUMO
Se estudió el estrés oxidativo en los pacientes diabéticos insulinodependientes con retinopatía diabética (RD) y sin ella, se evaluaron las especies reactivas de oxígeno, mediante la determinación de los niveles de malonildialdehído (MDA) y la actividad de las enzimas superóxido dismutasa (SOD) y catalasa (CAT). Se obtuvo una muestra poblacional integrada por 84 pacientes diabéticos insulinodependientes, divididos en 38 pacientes sin RD y 46 con RD, este último grupo se subdividió en 21 pacientes con RD no proliferativa y 25 con RD proliferativa (RDP). A todos los pacientes se les determinó en plasma: glucosa, hemoglobina glucosilada, fructosamina, SOD, CAT y MDA. Se observó que la característica clínica más sobresaliente fue el tiempo de evolución de la diabetes donde aquellos que presentaban RD tenían más de 15 años de evolución. Los pacientes diabéticos presentaron elevados niveles de peroxidación lipídica, que se incrementó con el grado de severidad de la RD y con el mal control metabólico a corto plazo. En conclusión, se comprobó que la DM se asocia a un estrés oxidativo, incrementado en los pacientes con RDP y en aquellos con mal control metabólico(AU)
The oxidative stress was studied in insulin-dependent diabetic patients with and without diabetic retinopathy (DR). The reactive oxygen species were evaluated by the determination of the levels of malondialdehyde (MDA) and the activity of the superoxide dismutase (SOD) and catalase (CAT) enzymes. A population sample composed of 84 insulin-dependent diabetic patients, divided into 38 patients without DR and 46 with DR was obtained. This last group was subdivided into 21 patients with non-proliferative DR and 25 with proliferative DR (PDR). Glucose, glycosylated hemoglobin, fructosamine, SOD, CAT and MDA were determined in plasma of all patients. It was observed that the most significant clinical characteristic was the time of evolution of diabetes, where those with DR had more than 15 years of evolution. The diabetic patients showed elevated levels of lipid peroxidation, which increased with the degree of severity of DR and with the poor short term metabolic control. To conclude, it was proved that DM is associated with an oxidative stress that is higher in patients with PDR and among those with a deficient metabolic control(AU)
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Humanos , Estresse Oxidativo , Diabetes Mellitus Tipo 1/etiologia , Retinopatia Diabética , Espécies Reativas de OxigênioRESUMO
Se comprobó experimentalmente en ratas la utilidad del Polypodium polypodiodes en la atenuación del efecto hepatotóxico provocado por el tetracloruro de carbono, que origina un incremento del nivel de los peróxidos lipídicos y las transaminasas sanguíneas. Se logró una disminución de la transaminasa glutamicopirúvica por debajo de los niveles normales con la administración del extracto de la planta a una dosis de 300 mg/kg de peso corporal. Los peróxidos lipídicos y la transaminasa glutamicooxalacética reducen su actividad, pero no notablemente, lo cual evidencia que el daño hepático persiste a pesar de la recuperación parcial. Los resultados fueron confirmados por un estudio anatomopatológico. Se determinó la toxicidad aguda por vía oral del extracto de la planta y se observó que la DL50 se encuentra por encima de 1 g/kg, por lo que puede considerarse esta preparación como relativamente inocua en los animales de experimentación
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Alanina Transaminase/análise , Aspartato Aminotransferases/análise , Tetracloreto de Carbono/efeitos adversos , Peroxidação de Lipídeos , Hepatopatias/induzido quimicamente , Plantas MedicinaisRESUMO
Se estudiaron 38 pacientes adultosambulatorios, seleccionados de la consulta de Arritmias del Instituto de Cardiologia y Cirugia Cardiovascular, los cuales recibian tratamiento con amiodarona oral por un periodo variable. Se realizaron determinaciones de parametros clinicos y marcadores enzimaticos de utilidad diagnostica para precisar las posibles alteraciones de metabolismo anergetico y lipidico, asi como para precisar las posibles alteraciones de metabolismo energetico y lipidico, asi como para estudiar la influencia de este farmaco sobre la enzima fosfolipasa A2 sistemica (PLA2). Ademas, se analizaron 5 indicadores del funcionamiento hepatico. En todos los casos se tomo como referencia el rango clinico normal de cada paramtero analizado, y se evidencio que aun a bajas dosis existen deficiencias en la disponibilidad de sustratos para la obtencion de energia, agudizada por la afectacion de la sintesis hepatica y la inhibicion de la PLA2, lo cual constituye un factor de riesgo para la insuficiencia cardiaca congestiva
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Humanos , Masculino , Feminino , Adulto , Amiodarona/efeitos adversos , Fígado/metabolismo , Metabolismo Energético , Fosfolipases A/metabolismo , Doses MínimasRESUMO
Se utilizó la enzima fosfolipasa A como modelo para el estudio del bloqueador de receptores de estrógenos 13R051 a las concentraciones de 30, 60, 80 y 360 mmol/L, con vistas a conocer a través de los cambios funcionales de la enzima los posibles procesos moleculares que pudieran explicar algunas de las propiedades farmacológicas del bloqueador. Se evidenció que la fosfolipasa A resultó un modelo de utilidad para el estudio de estos tipos de fármacos, y se demostró que el agente 13R051 fue capaz de activar a esta acilhidrolasa exhibiendo propiedades líticas. Los resultados cinéticos sugieren que el agente 13R051 estimula la actividad enzimática pero dentro de límites determinados, lo que representa una ventaja desde el punto de vista de su utilización clínica
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Fosfolipases A/farmacologiaRESUMO
Se realizó un estudio comparativo entre 2 hospitales de Ciudad de la Habana (A y B) con 2 poblaciones seleccionadas de pacientes geriátricos tratados cono psicofármacos (benzodiacepinas y antidepresivos tricíclicos). Se demostró que exístian diferencias significativas entre ambos centros asistenciales en cuanto a la duración del tratamiento para ambos tipos de fármacos. El hospital A presentó un mayor porcentaje de reacciones adversas, a pesar de que la dosificación y la duración del tratamiento para los psicotrópicos estudiados fue menor que en el hospital B, explicándose a partir de los mecanismos de acción correspondientes, las interacciones farmacológicas que se originan por administración simultánea de diferentes tipos de medicamentos