RESUMO
With the purpose of studying the effect of diphenylhydantoin on mandibular skeletal-unit growth, 28 male Wistar rats weighing 60.0 +/- 0.8 g were assigned to five different groups. One group received saline serving as normal controls; three others were injected intra peritoneally once daily with either 25, 50 or 100 mg/kg body wt diphenylhydantoin for 30 days; the fifth group was put on a restricted diet (20% below normal intake) for the same time. On day 31, the rats were killed by ether overdose and their mandibles were evaluated for differential skeletal-unit growth. Body-weight gain of diphenylhydantoin-injected rats was up to 24% less than controls, regardless of drug dose. Diet-restricted rats showed a similar difference. The amount of food consumed by diphenylhydantoin-injected rats was 21% less than that consumed by controls, regardless of drug doses. The concentration of alkaline phosphatase and haemoglobin in rats treated with 50 or 100 mg/kg diphenylhydantoin was lower than in controls and diet-restricted rats. However, plasma urea and total calcium were similar in diphenylhydantoin-treated rats and controls. Mean appetite quotient, and the efficiency of protein and energy utilization, did not appear to change in response to the particular diphenylhydantoin dose or to the restricted diet. Mandibular dimensions of rats injected with 25 or 50 mg/kg diphenylhydantoin were not statistically different from those of the control and diet-restricted groups. With using 100 mg/kg diphenylhydantoin for 30 days, the growth of symphysial and basal heights, condylar and angular lengths and condylar width was significantly less than in the control and diet-restricted groups. The remaining mandibular skeletal units did not exhibit significant differences from those of control and diet-restricted rats. The disharmonious growth of the mandible does not appear to depend on suboptimal energy intake, efficiency of protein-energy utilization, renal failure and anaemia, but would suggest a differential toxicological effect of diphenylhydantoin on the osseous component and/or its associated non-skeletal tissues.
Assuntos
Desenvolvimento Ósseo/efeitos dos fármacos , Mandíbula/efeitos dos fármacos , Mandíbula/crescimento & desenvolvimento , Fenitoína/toxicidade , Fosfatase Alcalina/sangue , Animais , Ingestão de Energia , Metabolismo Energético , Comportamento Alimentar/efeitos dos fármacos , Privação de Alimentos , Hemoglobinas/análise , Masculino , Côndilo Mandibular/efeitos dos fármacos , Côndilo Mandibular/crescimento & desenvolvimento , Proteínas/metabolismo , Ratos , Ratos Wistar , Aumento de Peso/efeitos dos fármacosRESUMO
With the purpose of studying the effect of diphenylhydantoin (DPH) administration on the growth of the functional components of the rat skull, male Wistar rats weighing 61.6 +/- 0.6g were assigned to 1 out of 4 different groups. One of them received saline and was taken as normal control. The others were injected once daily with 25, 50 or 100 mg/kg of b.w. of DPH i.p. for 20 days. Another group of rats was put under a restricted diet (RD) (20% of normal intake) for the same time for evaluation of the cranial dimensions. On day 21 the rats were killed by ether overdose and fifteen cranial dimensions were evaluated as previously described employing Pucciarelli's method. The body weight gain of DPH injected rats was up to 20.7% lower independently of drug dose. The rats of the RD group showed a similar reduction. The amount of food consumed by DPH rats was 16% lower than that consumed by controls independently of drug doses. The concentration of alkaline phosphatase and hemoglobin in rats treated with 50 or 100 mg/kg b.w. of DPH was lower than in controls and RD-animals. However, urea and total calcium in plasma were unchanged in DPH-treated rats as compared to controls. Mean appetite quotient, efficiency of protein and energy utilization did not appear to change in response to the treatment with DPH or maintained under a restricted diet. The cranial dimensions of rats, injected with 25 mg/kg b.w. of DPH were not statistically different from those of the control and RD-groups. When the dose of DPH injected was 50 mg/kg b.w. the neurocranial width and height and the spachnocranial length were significantly lower than controls and RD-values. Moreover, all the dimensions corresponding to neurocranium and splachnocranium (width, height and length) of the rats injected with 100 mg/kg b.w. of DPH were significantly lower than those of control and RD-groups. The disharmonius growth of the skull do not appear to be dependent on suboptimal energy intake, efficiency of protein, energy utilization renal failure and anemia.
Assuntos
Anticonvulsivantes/farmacologia , Fenitoína/farmacologia , Crânio/efeitos dos fármacos , Animais , Masculino , Ratos , Ratos Wistar , Crânio/crescimento & desenvolvimento , Crânio/fisiologiaRESUMO
With the purpose of studying the effect of diphenylhydantoin (DPH) administration on the growth of the functional components of the rat skull, male Wistar rats weighing 61.6 + 0.6g were assigned to 1 out of 4 different groups. One of them received saline and was taken as normal control. The others were injected once daily with 25,50 or 100 mg/Kg of b.w. of DPH i.p. for 20 days. Another group of rats was put under a restricted diet (RD) (20 percent of normal intake) for the same time for evaluation of the cranial dimensions. On day 21, the rats were killed by ether overdose and fifteen cranial dimensions were evaluated as peviously described employing Pucciarelli's method. The body weight gain of DPH injected rats was up to 20.7 percent lower independently of drug dose. The rats of the RD group showed a similar reduction. The amount of food consumed by DPH rats was 16 percent lower than that consumed by controls independently of drug doses. The concentration of alkaline phosphatase and hemoglobin in rats treated with 50 or 100 mg/kg b.w. of DPH was lower than in controls and RD-animals. However, urea and total calcium in plasma were unchanged in DPH-treated rats as compared to controls. Mean appetite quotient, efficiency of protein and energy utilization did not appear to change in response to the treatment with DPH or maintained under a restricted diet. That cranial dimensions of rats, injected with 25mg/kg b.w. of DPH were not statistically different from those of the control and RD-groups. When the dose of DPH injected was 50 mg/kg b.w. the neurocranial width an height and the spachnocranial length were significantly lower than controls and RD-values. Moreover, all the dimensions corresponding to neurocranium and splachnocranium (width, height and length) of the rats injected with 100 mg/kg b.w. of DPH were significantly lower than those of control and RD-groups. The disharmonius growth of the skull do not appear to be dependent on suboptimal energy intake, efficiency of protein, energy utilization renal failure and anemia.
Assuntos
Ratos , Animais , Masculino , Anticonvulsivantes/farmacologia , Fenitoína/farmacologia , Crânio/efeitos dos fármacos , Ratos Wistar , Crânio/crescimento & desenvolvimento , Crânio/fisiologiaRESUMO
With the purpose of studying the effect of diphenylhydantoin (DPH) administration on the growth of the functional components of the rat skull, male Wistar rats weighing 61.6 + 0.6g were assigned to 1 out of 4 different groups. One of them received saline and was taken as normal control. The others were injected once daily with 25,50 or 100 mg/Kg of b.w. of DPH i.p. for 20 days. Another group of rats was put under a restricted diet (RD) (20 percent of normal intake) for the same time for evaluation of the cranial dimensions. On day 21, the rats were killed by ether overdose and fifteen cranial dimensions were evaluated as peviously described employing Pucciarellis method. The body weight gain of DPH injected rats was up to 20.7 percent lower independently of drug dose. The rats of the RD group showed a similar reduction. The amount of food consumed by DPH rats was 16 percent lower than that consumed by controls independently of drug doses. The concentration of alkaline phosphatase and hemoglobin in rats treated with 50 or 100 mg/kg b.w. of DPH was lower than in controls and RD-animals. However, urea and total calcium in plasma were unchanged in DPH-treated rats as compared to controls. Mean appetite quotient, efficiency of protein and energy utilization did not appear to change in response to the treatment with DPH or maintained under a restricted diet. That cranial dimensions of rats, injected with 25mg/kg b.w. of DPH were not statistically different from those of the control and RD-groups. When the dose of DPH injected was 50 mg/kg b.w. the neurocranial width an height and the spachnocranial length were significantly lower than controls and RD-values. Moreover, all the dimensions corresponding to neurocranium and splachnocranium (width, height and length) of the rats injected with 100 mg/kg b.w. of DPH were significantly lower than those of control and RD-groups. The disharmonius growth of the skull do not appear to be dependent on suboptimal energy intake, efficiency of protein, energy utilization renal failure and anemia. (AU)