RESUMO
OBJECTIVES: The aim of this study was to determine and compare the frequency of bacterial leakage of Streptococcus sanguinis biotype II along the implant-abutment interface between two systems of morse taper dental implants. Different methods of activation of the taper abutments were used: tapped-in (Bicon) and screwed-in (Ankylos). MATERIALS AND METHODS: Twenty sterile assemblies were used and attached, 10 Bicon and 10 Ankylos implants, according to manufacturers' specifications. They were then totally immersed within 20 test tubes containing a sterile nutrient solution brain-heart infusion (BHI). The internal part of the 20 implants was previously inoculated with 0.1 microl of S. sanguinis II (ATCC 10557) and then connected to the respective abutments. The assemblies were incubated under anaerobic conditions for 14 days in an autoclave at 37 degrees C. They were monitored daily for solution cloudiness resultant from microbial leakage on the interface of the assemblies. For statistical analysis, the Fisher test was applied and significance was assigned at the 5% level. RESULTS: There was solution cloudiness, indicating the finding of bacterial growth inside two Bicon assemblies and two Ankylos assemblies 48 h after incubation. Microbial leakage was further substantiated by testing the suspension for the presence of Streptococcus sp. None of the sterility controls were contaminated. The frequency of bacterial leakage along the implant-abutment interface, with the two different morse taper implant systems, was 20% of the assemblies of each system. There were no statistical differences between them. CONCLUSION: Irrespective of which of the two morse taper implant connection systems of activation was analyzed, tapped-in (Bicon) or screwed-in (Ankylos), this in vitro experiment showed bacterial leakage along the implant-abutment interface.
Assuntos
Dente Suporte/microbiologia , Implantes Dentários/microbiologia , Infiltração Dentária , Planejamento de Prótese Dentária , Nefelometria e Turbidimetria , Streptococcus sanguis/isolamento & purificaçãoRESUMO
Pterotaenia fasciata is commonly recorded in rural areas in Argentina, but during a Diptera survey study developed in a reservoir which retains storm water from polluted canals in an urban area of Taboão da Serra municipality, SP, Brazil, we could capture P. fasciata adults. Enteric bacteria Escherichia coli T. Escherich, 1885 and Proteus sp. were isolated from P. fasciata collected in traps inside the reservoir and around it. Fecal coliforms and E. coli were found in the water of the reservoir. These records suggest that a high abundance of this species at urban areas with inadequate sewage canals should reveal these muscoid dipterans as mechanical vectors of enteric bacteria.
Pterotaenia fasciata é encontrada freqüentemente em áreas rurais na Argentina, mas durante um estudo de levantamento de Diptera em um reservatório de retenção de enchentes em uma área urbana do município de Taboão da Serra, SP, Brasil, foram capturados adultos de P. fasciata. As enterobactérias Escherichia coli T. Escherich, 1885 e Proteus sp. foram isoladas de P. fasciata coletada em armadilhas no reservatório e em seu entorno. Coliformes fecais e E. coli foram encontrados na água do reservatório. Esses registros sugerem que a alta abundância dessa espécie em áreas urbanas sem saneamento básico poderia indicar esses dípteros muscóides como vetores mecânicos de enterobactérias.