RESUMO

The aim of this study was to analyze the demographic, clinical, and hematological aspects of the population in a dog shelter located in the municipality of Lavras, Brazil. All animals were microchipped and evaluated by veterinarians. Whole blood samples were obtained from 329 dogs in the months of July-August 2019 and from 310 dogs in the months of January-February 2020. Most of the dogs were of mixed breed, received anti-rabies and polyvalent vaccines (100%), were dewormed (100%), and were spayed/neutered (98.59%), with a predominance of adult (86.51%), short-hair (67.51%), normal body condition (65.57%), medium-size (62.57%), and female (62.36%). The main clinical alterations detected were enlarged lymph nodes (38.69%), skin lesions (31.50%), overweight (23.32%), obesity (6.07%), elevated temperature (17.05%), and ear secretion (15.72%). Regarding hematological alterations, thrombocytopenia (36.31%), leukopenia (15.92%), anemia with decreased hemoglobin values (10.60%), hematocrit (9.70%), and red blood cells (5.14%) were observed. Most of the shelter dogs were apparently healthy, but specific measures for nutritional, dermatological, otological and disease management should be implemented once the health changes are verified, as they impact the general state of the population and adoptions.

RESUMO

Background: Esophageal neoplasms are rarely reported in cats. The frequency rate is less than 0.5% and those neoplasms are usually malignant. Esophageal squamous cell carcinoma (SCC) is an idiopathic epithelial neoplasm, invasive and metastatic that can induce partial or complete obstruction of the esophageal lumen. There is no breed or sex predisposition, and it is more common in cats over 8-years-old. Esophageal SCC is more frequent in the middle third of the esophagus. The prognosis is poor, as the cats are usually diagnosed at an advanced stage. This report aims to describe clinical, endoscopic, radiographic, and pathological features of two cases of esophageal squamous cell carcinoma in cats. Cases: A 11-year-old neutered male cat presenting regurgitation, weight loss, anorexia and dyspnea was referred to veterinary internal medicine care. Simple and contrast-enhanced radiographic images of the cervical and thoracic regions showed an alveolar pattern in the cranial lung lobes and signs of esophageal lumen irregularity and dilatation in the mediastinum topography. The upper digestive endoscopy showed a dilated esophageal lumen, and an irregular mass was observed in the thoracic esophagus involving the entire esophageal circumference. Biopsy fragments were collected, and the histopathological result was compatible with squamous cell carcinoma. The second case was a 10-year-old neutered male cat presenting hyporexia, regurgitation, dyspnea, tachypnea, and abnormal breath sounds. The ultrasound of the chest showed 3 amorphous hypoechogenic and heterogeneous areas in the right and left hemithorax between parietal and visceral pleura. The cytological examination was compatible with a malignant epithelial tumor. The patient died 3 months after the onset of clinical signs. At gross exam, it was observed a friable, irregular, and ulcerated mass of 5.0 x 3.0 cm in the middle third of the esophagus...

Assuntos

Animais , Gatos , Carcinoma de Células Escamosas do Esôfago/diagnóstico por imagem , Carcinoma de Células Escamosas do Esôfago/patologia , Carcinoma de Células Escamosas do Esôfago/veterinária , Esôfago/patologia , Metástase Neoplásica

RESUMO

Background: Gastric cancer is one of the most incident types of cancer worldwide and presents high mortality rates and poor prognosis. MYC oncogene overexpression is a key event in gastric carcinogenesis and it is known that its protein positively regulates CDC25B expression which, in turn, plays an essential role in the cell division cycle progression. Menadione is a synthetic form of vitamin K that acts as a specific inhibitor of the CDC25 family of phosphatases. Methods: To better understand the menadione mechanism of action in gastric cancer, we evaluated its molecular and cellular effects in cell lines and in Sapajus apella, nonhuman primates from the new world which had gastric carcinogenesis induced by N-Methyl-N-nitrosourea. We tested CDC25B expression by western blot and RT-qPCR. In-vitro assays include proliferation, migration, invasion and flow cytometry to analyze cell cycle arrest. In in-vivo experiments, in addition to the expression analyses, we followed the preneoplastic lesions and the tumor progression by ultrasonography, endoscopy, biopsies, histopathology and immunohistochemistry. Results: Our tests demonstrated menadione reducing CDC25B expression in vivo and in vitro. It was able to reduce migration, invasion and proliferation rates, and induce cell cycle arrest in gastric cancer cell lines. Moreover, our in-vivo experiments demonstrated menadione inhibiting tumor development and progression. Conclusions: We suggest this compound may be an important ally of chemotherapeutics in the treatment of gastric cancer. In addition, CDC25B has proven to be an effective target for investigation and development of new therapeutic strategies for this malignancy.

RESUMO

Background: Articular cartilage has a limited capacity for regeneration and of the various treatments proposed, none have reached appropriate therapeutic effectiveness. This study aimed to evaluate autogenous osteochondral grafts in intact or macerated format, in association with or without insulin-like growth factor type-1 (IGF-1) in the repair of osteochondral defects induced in the femoral trochlear groove of rabbits.Materials, Methods & Results: Seventeen healthy White New Zealand rabbits were selected for this study. The rabbits were female, six months old, and had an average body weight of 4.5 kg. All 34 stifle joints were subjected to autogenous osteochondral grafting in the femoral trochlear groove. The joints were divided into four groups designated as intact osteochondral graft with IGF-1 (INT + IGF), intact osteochondral graft with physiological solution (INT + FIS), macerated osteochondral graft with IGF-1 (MAC + IGF), and macerated osteochondral graft with physiological solution (MAC + FIS). Serial evaluations were performed by orthopedic and radiographic examination. After 6 and 12 weeks postoperatively, the grafted area was subjected to macroscopic, histological, and immunohistochemical analyses. Although no statistically significant differences were found between the groups in relation to clinical, macroscopic, histological, and immunohistochemical aspects, a tendency of IGF-1 to promote tissue repair was evident. In the radiographic evaluation, the articular surface and the recipient site in both groups with IGF-1 showed significantly more effective filling (P ≤ 0.05). Regardless of the group, collagen type 2 production, as assessed by immunohistochemistry, was found to be appropriate on the grafted articular surface.[...](AU)

Assuntos

Animais , Feminino , Coelhos , Cartilagem Articular/patologia , Transplante Ósseo/veterinária , Transplante Autólogo/veterinária , Fator de Crescimento Insulin-Like I/uso terapêutico

RESUMO

Background: Articular cartilage has a limited capacity for regeneration and of the various treatments proposed, none have reached appropriate therapeutic effectiveness. This study aimed to evaluate autogenous osteochondral grafts in intact or macerated format, in association with or without insulin-like growth factor type-1 (IGF-1) in the repair of osteochondral defects induced in the femoral trochlear groove of rabbits.Materials, Methods & Results: Seventeen healthy White New Zealand rabbits were selected for this study. The rabbits were female, six months old, and had an average body weight of 4.5 kg. All 34 stifle joints were subjected to autogenous osteochondral grafting in the femoral trochlear groove. The joints were divided into four groups designated as intact osteochondral graft with IGF-1 (INT + IGF), intact osteochondral graft with physiological solution (INT + FIS), macerated osteochondral graft with IGF-1 (MAC + IGF), and macerated osteochondral graft with physiological solution (MAC + FIS). Serial evaluations were performed by orthopedic and radiographic examination. After 6 and 12 weeks postoperatively, the grafted area was subjected to macroscopic, histological, and immunohistochemical analyses. Although no statistically significant differences were found between the groups in relation to clinical, macroscopic, histological, and immunohistochemical aspects, a tendency of IGF-1 to promote tissue repair was evident. In the radiographic evaluation, the articular surface and the recipient site in both groups with IGF-1 showed significantly more effective filling (P ≤ 0.05). Regardless of the group, collagen type 2 production, as assessed by immunohistochemistry, was found to be appropriate on the grafted articular surface.[...]

Assuntos

Feminino , Animais , Coelhos , Cartilagem Articular/patologia , Fator de Crescimento Insulin-Like I/uso terapêutico , Transplante Autólogo/veterinária , Transplante Ósseo/veterinária

RESUMO

Background: Pyothorax is characterized by the accumulation of septic purulent fluid within the pleural space. Most of the times, it is the true identified infection way in only 2 to 22% of dog cases. Reports show that the most common cause is the migration of grass edges and plant materials, mainly in regions of California, USA. The current study reports an unusual case of a Cassia fistula pod (Brazilian Acácia Imperial), of around 10x3 cm long, causing chronic Pyothorax in a Border Collie female dog. Case: It was admitted in a Teaching Veterinary Hospital a 2-year-old female Border Collie, weighing 16.5 kg. The complaint was producing of severe cough, dyspnoea, hyporexia and loss of weight in the past three months. The animal could have been horse-kicked, as it is used to herding. In the physical examination, it was detected bilateral thick lung crepitations, more evident in the ventral skull area, dyspnoea, tachypnea, fever, splenomegaly and low body condition score (3/9). The laboratory tests revealed nonregenerative anemia and leukemoid reaction. The chest x-ray showed intense pulmonary opacification and free fluids in the pleural space along with a heterogenic cylindrical image of the mixed radiopacity. This image was then confirmed as the intrathoracic foreing body by the use of a computerized tomography. In this exam it was also possible to observe the damage of part of the middle and inferior right lung lobe. The cytology of the pleural effusion found mixed inflammation and coccoid bacteria, and the microbiological culture Streptococcus sp., and Escherichia coli. The dog underwent thoracotomy in order to remove the foreign body, which was later identified as being a Cassia fistula pod (Brazilian Acácia Imperial), of around 10x3 cm long. Due to the intense lung damage, a right pneumonectomy was required. After 30 days the animal was fully recovered.[...](AU)

Assuntos

Animais , Cães , Empiema Pleural/veterinária , Corpos Estranhos/veterinária , Cassia , Cavidade Torácica , /veterinária

RESUMO

Background: The splenic torsion is a rare condition in dogs, especially when it is not associated with gastric dilatation and volvulus. Abdominal ultrasonography has been reported as an accurate diagnostic method in cases of splenic torsion suspicions. Splenomegaly, decreased echogenicity of the parenchyma of the spleen and hypoechoic appearance or “lace” are suggestive sonographic findings, although the definitive diagnosis is given through exploratory laparotomy. This study reports a case of splenic torsion followed by abdominal trauma and eventration in a dog, highlighting the importance of the ultrasound examination. Case: An adult dog without a defined breed pattern that weighed 8.6 kg was referred to the Veterinary Hospital of the Federal University of Lavras (Universidade Federal de Lavras - UFLA) with a history of trauma caused by having been run over by a car 24 h earlier. The animal exhibited right inguinal region swelling with hematoma and a loss of local muscular tension. An abdominal ultrasound indicated that the spleen was displaced to the right inguinal region and that a portion of the spleen was included in the animals abdominal eventration. The spleen possessed an irregular surface with increased dimensions and diffusely decreased echogenicity and multiple parallel echogenic lines (in the reticular aspect). Abrupt differences in the echogenic textures of the spleen were identified in certain images. Color Doppler assessments revealed no blood flow. Based on the aforementioned ultrasound findings, an exploratory laparotomy was indicated. The laparotomy confirmed the presence of right inguinal abdominal muscle rupture with dislocation, eventration, and splenic torsion. Discussion: Splenic torsion may be most frequently reported in cases involving large and giant male dogs because this condition is typically related to dilatation syndrome and gastric volvulus, which are more prevalent among large and giant male dogs than among other dogs […](AU)

Assuntos

Animais , Cães , Baço/lesões , Traumatismos Abdominais/veterinária , Traumatismos Abdominais , Ultrassonografia Doppler/veterinária

RESUMO

Background: The largest buffalo herd in Brazil is located on the Island of Marajó, in the State of Pará, northern Brazil. The pastures of the Island of Marajó consist of low quality graminaceous plants, which are generally poor in protein and mineral content. Unbalanced diets associated with low quality pastures are responsible for latent, sub-clinical diseases and metabolic disorders in bovines which affect bone health, especially in periods such as pregnancy and lactation. The purpose of this study was to point out and to describe the radiographic bone changes of buffalos with low body index bred in extensive system and intended for slaughter on the Island of Marajó, Brazil.Materials, Methods & Results: Radiographic examinations of anatomical pieces were obtained from 34 animals of buffalo species, with no distinction of gender, age, or breed. The animals were selected among those that were in the stockyard waiting for slaughtering for the obtainment of the anatomical pieces. For this selection, low physical condition was considered, which mainly included individuals with body condition indexes (ICC) of 1 and 2, on a scale of 1 to 5. From this selection, 98 anatomical pieces were obtained, which included: 28 sets of ribs, 20 femurs, 26 metacarpus, 7 mandibles, 3 radius and ulnas, 4 sets of vertebrae, 4 sets of metacarpus and phalanges, 1 tarsus and 1 set of tarsus and metatarsus. All the pieces were separated, identified, packed in plastic bag and forwarded to the radiographic study. At least one radiographic projection was obtained of each anatomical piece. These were identified, manually processed and stored for subsequent assessment. A single observer, in order to identify and to describe the bone radiographic changes, subjectively performed the radiographic assessment.[...](AU)

Assuntos

Animais , Búfalos/anatomia & histologia , Radiografia/veterinária , Osso e Ossos/patologia , Osteoporose/veterinária , Fraturas Ósseas/veterinária , Potássio na Dieta , Cobre/administração & dosagem , Costelas/lesões

RESUMO

Background: The splenic torsion is a rare condition in dogs, especially when it is not associated with gastric dilatation and volvulus. Abdominal ultrasonography has been reported as an accurate diagnostic method in cases of splenic torsion suspicions. Splenomegaly, decreased echogenicity of the parenchyma of the spleen and hypoechoic appearance or “lace” are suggestive sonographic findings, although the definitive diagnosis is given through exploratory laparotomy. This study reports a case of splenic torsion followed by abdominal trauma and eventration in a dog, highlighting the importance of the ultrasound examination. Case: An adult dog without a defined breed pattern that weighed 8.6 kg was referred to the Veterinary Hospital of the Federal University of Lavras (Universidade Federal de Lavras - UFLA) with a history of trauma caused by having been run over by a car 24 h earlier. The animal exhibited right inguinal region swelling with hematoma and a loss of local muscular tension. An abdominal ultrasound indicated that the spleen was displaced to the right inguinal region and that a portion of the spleen was included in the animal’s abdominal eventration. The spleen possessed an irregular surface with increased dimensions and diffusely decreased echogenicity and multiple parallel echogenic lines (in the reticular aspect). Abrupt differences in the echogenic textures of the spleen were identified in certain images. Color Doppler assessments revealed no blood flow. Based on the aforementioned ultrasound findings, an exploratory laparotomy was indicated. The laparotomy confirmed the presence of right inguinal abdominal muscle rupture with dislocation, eventration, and splenic torsion. Discussion: Splenic torsion may be most frequently reported in cases involving large and giant male dogs because this condition is typically related to dilatation syndrome and gastric volvulus, which are more prevalent among large and giant male dogs than among other dogs […]

Assuntos

Animais , Cães , Baço/lesões , Traumatismos Abdominais , Traumatismos Abdominais/veterinária , Ultrassonografia Doppler/veterinária

RESUMO

Background: Pyothorax is characterized by the accumulation of septic purulent fluid within the pleural space. Most of the times, it is the true identified infection way in only 2 to 22% of dog cases. Reports show that the most common cause is the migration of grass edges and plant materials, mainly in regions of California, USA. The current study reports an unusual case of a Cassia fistula pod (Brazilian Acácia Imperial), of around 10x3 cm long, causing chronic Pyothorax in a Border Collie female dog. Case: It was admitted in a Teaching Veterinary Hospital a 2-year-old female Border Collie, weighing 16.5 kg. The complaint was producing of severe cough, dyspnoea, hyporexia and loss of weight in the past three months. The animal could have been horse-kicked, as it is used to herding. In the physical examination, it was detected bilateral thick lung crepitations, more evident in the ventral skull area, dyspnoea, tachypnea, fever, splenomegaly and low body condition score (3/9). The laboratory tests revealed nonregenerative anemia and leukemoid reaction. The chest x-ray showed intense pulmonary opacification and free fluids in the pleural space along with a heterogenic cylindrical image of the mixed radiopacity. This image was then confirmed as the intrathoracic foreing body by the use of a computerized tomography. In this exam it was also possible to observe the damage of part of the middle and inferior right lung lobe. The cytology of the pleural effusion found mixed inflammation and coccoid bacteria, and the microbiological culture Streptococcus sp., and Escherichia coli. The dog underwent thoracotomy in order to remove the foreign body, which was later identified as being a Cassia fistula pod (Brazilian Acácia Imperial), of around 10x3 cm long. Due to the intense lung damage, a right pneumonectomy was required. After 30 days the animal was fully recovered.[...]

Assuntos

Animais , Cães , Cassia , Cavidade Torácica , Corpos Estranhos/veterinária , Empiema Pleural/veterinária

RESUMO

Background: The largest buffalo herd in Brazil is located on the Island of Marajó, in the State of Pará, northern Brazil. The pastures of the Island of Marajó consist of low quality graminaceous plants, which are generally poor in protein and mineral content. Unbalanced diets associated with low quality pastures are responsible for latent, sub-clinical diseases and metabolic disorders in bovines which affect bone health, especially in periods such as pregnancy and lactation. The purpose of this study was to point out and to describe the radiographic bone changes of buffalos with low body index bred in extensive system and intended for slaughter on the Island of Marajó, Brazil.Materials, Methods & Results: Radiographic examinations of anatomical pieces were obtained from 34 animals of buffalo species, with no distinction of gender, age, or breed. The animals were selected among those that were in the stockyard waiting for slaughtering for the obtainment of the anatomical pieces. For this selection, low physical condition was considered, which mainly included individuals with body condition indexes (ICC) of 1 and 2, on a scale of 1 to 5. From this selection, 98 anatomical pieces were obtained, which included: 28 sets of ribs, 20 femurs, 26 metacarpus, 7 mandibles, 3 radius and ulnas, 4 sets of vertebrae, 4 sets of metacarpus and phalanges, 1 tarsus and 1 set of tarsus and metatarsus. All the pieces were separated, identified, packed in plastic bag and forwarded to the radiographic study. At least one radiographic projection was obtained of each anatomical piece. These were identified, manually processed and stored for subsequent assessment. A single observer, in order to identify and to describe the bone radiographic changes, subjectively performed the radiographic assessment.[...]

Assuntos

Animais , Búfalos/anatomia & histologia , Cobre/administração & dosagem , Fraturas Ósseas/veterinária , Osso e Ossos/patologia , Osteoporose/veterinária , Potássio na Dieta , Radiografia/veterinária , Costelas/lesões

RESUMO

Background: The multiple myeloma is a neoplasia characterized by the uncontrolled proliferation of plasma cells (plasmacytes) in the bone marrow and in other tissues. The infiltration of the neoplasia cells associated to the high level of anomalous immunoglobulin production (M protein) results in a variety of clinic-pathologic anomalies. It is a rare disease in dogs, corresponding to 0.3% of all malignant neoplasia and 2% of the hematopoietic, with few literature descriptions. So, the present paper aims at properly report a multiple myeloma in a dog of non-defined breed, emphasizing the clinic, laboratorial, radiographic and pathologic aspects. Case: In a Veterinary Teaching Hospital, an 11-year-old dog of non-defined breed was admitted, weighing 10.8 kg of body mass. The clinic history was claudication of the right thoracic member, hyporexia and lethargy in the past 20 days. The main abnormalities in the physical examination were holosystolic cardiac murmur III/VI on mitral focus, and high sensibility to touch in the right humerus. Laboratory tests showed pancytopenia, serum hypercalcemia and Bence-Jones proteinuria. Radiographic examination confirmed polyostotic punctate osteolysis on the right humerus; pelvic, femurs and vertebrae bones from L2 to L7, on generalized condition. Cytology by aspiration puncture from the left femur marrow bone did not confirm neoplasia cells [...](AU)

Assuntos

Animais , Cães , Mieloma Múltiplo/patologia , Mieloma Múltiplo , Mieloma Múltiplo/veterinária , Medula Óssea , Neoplasias Ósseas/veterinária

RESUMO

Background: The multiple myeloma is a neoplasia characterized by the uncontrolled proliferation of plasma cells (plasmacytes) in the bone marrow and in other tissues. The infiltration of the neoplasia cells associated to the high level of anomalous immunoglobulin production (M protein) results in a variety of clinic-pathologic anomalies. It is a rare disease in dogs, corresponding to 0.3% of all malignant neoplasia and 2% of the hematopoietic, with few literature descriptions. So, the present paper aims at properly report a multiple myeloma in a dog of non-defined breed, emphasizing the clinic, laboratorial, radiographic and pathologic aspects. Case: In a Veterinary Teaching Hospital, an 11-year-old dog of non-defined breed was admitted, weighing 10.8 kg of body mass. The clinic history was claudication of the right thoracic member, hyporexia and lethargy in the past 20 days. The main abnormalities in the physical examination were holosystolic cardiac murmur III/VI on mitral focus, and high sensibility to touch in the right humerus. Laboratory tests showed pancytopenia, serum hypercalcemia and Bence-Jones proteinuria. Radiographic examination confirmed polyostotic punctate osteolysis on the right humerus; pelvic, femurs and vertebrae bones from L2 to L7, on generalized condition. Cytology by aspiration puncture from the left femur marrow bone did not confirm neoplasia cells [...]

Assuntos

Animais , Cães , Medula Óssea , Mieloma Múltiplo , Mieloma Múltiplo/patologia , Mieloma Múltiplo/veterinária , Neoplasias Ósseas/veterinária

RESUMO

Background: Traumatic causes is the main factor for bone fractures. Femoral fractures are common in dogs and cats and usually require internal fixation for bone healing. However, there is little described about fractures in wild animals. Diaphyseal fractures of long bones generally involve complex surgical procedures for maintaining of the alignment and length of the bone during its consolidation. The maned wolf (Crysocyon brachyurus) is a canid found in the Brazilian cerrado and solitary habit. This species is among those threatened with extinction. This study reports the surgical treatment in a femoral fracture in a maned wolf using an interlocking nail-plate combination.Case: A young maned wolf, 36 kg intact male, was admitted to the veterinary hospital due to running over. The animal did not weight bear on right pelvic limb. Under anesthesia with ketamina (15 mg/kg) and midazolan (0,3 mg/kg), orthopedic examination was performed. There was severe swelling in the proximal region of the pelvic limb and abnormal mobility in the middle third of the right femur. Radiographic examination showed fragmented and oblique fracture in the right femoral shaft. Surgery for internal fracture fixation was required. The distal segment of the fracture had a fragment with long oblique line, similar to a bevel, and two wires steel cerclage of 1.0 mm were used to stabilize it. The medullary can...(AU)

Assuntos

Animais , Lobos/cirurgia , Fraturas do Fêmur/cirurgia , Fraturas do Fêmur/veterinária , Consolidação da Fratura , Placas Ósseas/veterinária , Animais Selvagens/cirurgia , Dispositivos de Fixação Cirúrgica/veterinária

RESUMO

Background: Spondylodiscitis is an inflammation that is characterized by the involvement of one and more intervertebral discs and adjacent vertebral bodies. The clinical manifestations of spondylodiscitis are nonspecific and vary with the site and extent of injury, however this kind of lesion is poorly known between free-ranging cervids. In this sense, the presente study aimed to describe the occurrence, clinical, radiographic and anatomopathological findings related to a lumbar abscess accompanied by spondylodiscitis in a free-ranging female red brocket (Mazama americana) from Eastern Brazilian Amazon. Case: An approximately five-month old, 3,7 kg, female red brocket (Mazama americana), was received at the Veterinary Hospital of the Federal Rural University of Amazônia, in the municipality of Belém, in the State of Pará (Northern Brazil) for clinical care. The animal was rescued from the wild by the Brazilian Institute of Environment and Renewable Natural and presented lethargy, pelvic limb paralysis with proprioceptive deficits. The clinical signs observed suggested a spinal cord injury in the thoracolumbar region and the animal was sent to radiographic examination and myelography performed on the ventrodorsal and lateral projections of the vertebrae and spinal cord. The hemogram revealed anemia (8.74 × 106 cells/mm3) and leukocytosis (29.65 × 103 cells/mm3) with...(AU)

Assuntos

Animais , Compressão da Medula Espinal/diagnóstico por imagem , Compressão da Medula Espinal/veterinária , Papagaios , Discite/veterinária , Medula Espinal/patologia , Abscesso/veterinária , Músculos Paraespinais , Mielografia/veterinária

RESUMO

Background: Traumatic causes is the main factor for bone fractures. Femoral fractures are common in dogs and cats and usually require internal fixation for bone healing. However, there is little described about fractures in wild animals. Diaphyseal fractures of long bones generally involve complex surgical procedures for maintaining of the alignment and length of the bone during its consolidation. The maned wolf (Crysocyon brachyurus) is a canid found in the Brazilian cerrado and solitary habit. This species is among those threatened with extinction. This study reports the surgical treatment in a femoral fracture in a maned wolf using an interlocking nail-plate combination.Case: A young maned wolf, 36 kg intact male, was admitted to the veterinary hospital due to running over. The animal did not weight bear on right pelvic limb. Under anesthesia with ketamina (15 mg/kg) and midazolan (0,3 mg/kg), orthopedic examination was performed. There was severe swelling in the proximal region of the pelvic limb and abnormal mobility in the middle third of the right femur. Radiographic examination showed fragmented and oblique fracture in the right femoral shaft. Surgery for internal fracture fixation was required. The distal segment of the fracture had a fragment with long oblique line, similar to a bevel, and two wires steel cerclage of 1.0 mm were used to stabilize it. The medullary can...

Assuntos

Animais , Consolidação da Fratura , Fraturas do Fêmur/cirurgia , Fraturas do Fêmur/veterinária , Lobos/cirurgia , Placas Ósseas/veterinária , Animais Selvagens/cirurgia , Dispositivos de Fixação Cirúrgica/veterinária

RESUMO

Background: Spondylodiscitis is an inflammation that is characterized by the involvement of one and more intervertebral discs and adjacent vertebral bodies. The clinical manifestations of spondylodiscitis are nonspecific and vary with the site and extent of injury, however this kind of lesion is poorly known between free-ranging cervids. In this sense, the presente study aimed to describe the occurrence, clinical, radiographic and anatomopathological findings related to a lumbar abscess accompanied by spondylodiscitis in a free-ranging female red brocket (Mazama americana) from Eastern Brazilian Amazon. Case: An approximately five-month old, 3,7 kg, female red brocket (Mazama americana), was received at the Veterinary Hospital of the Federal Rural University of Amazônia, in the municipality of Belém, in the State of Pará (Northern Brazil) for clinical care. The animal was rescued from the wild by the Brazilian Institute of Environment and Renewable Natural and presented lethargy, pelvic limb paralysis with proprioceptive deficits. The clinical signs observed suggested a spinal cord injury in the thoracolumbar region and the animal was sent to radiographic examination and myelography performed on the ventrodorsal and lateral projections of the vertebrae and spinal cord. The hemogram revealed anemia (8.74 × 106 cells/mm3) and leukocytosis (29.65 × 103 cells/mm3) with...

Assuntos

Animais , Compressão da Medula Espinal/diagnóstico por imagem , Compressão da Medula Espinal/veterinária , Discite/veterinária , Medula Espinal/patologia , Papagaios , Abscesso/veterinária , Mielografia/veterinária , Músculos Paraespinais

Assuntos

Canidae , Fraturas Ósseas/veterinária , Membro Posterior/diagnóstico por imagem , Ligamento Cruzado Posterior/lesões , Animais , Fixação Interna de Fraturas/veterinária , Fraturas Ósseas/diagnóstico por imagem , Fraturas Ósseas/cirurgia , Masculino , Ligamento Cruzado Posterior/diagnóstico por imagem , Radiografia , Joelho de Quadrúpedes/diagnóstico por imagem , Joelho de Quadrúpedes/patologia

RESUMO

The evolution of gastric carcinogenesis remains largely unknown. We established two gastric carcinogenesis models in New-World nonhuman primates. In the first model, ACP03 gastric cancer cell line was inoculated in 18 animals. In the second model, we treated 6 animals with N-methyl-nitrosourea (MNU). Animals with gastric cancer were also treated with Canova immunomodulator. Clinical, hematologic, and biochemical, including C-reactive protein, folic acid, and homocysteine, analyses were performed in this study. MYC expression and copy number was also evaluated. We observed that all animals inoculated with ACP03 developed gastric cancer on the 9(th) day though on the 14(th) day presented total tumor remission. In the second model, all animals developed pre-neoplastic lesions and five died of drug intoxication before the development of cancer. The last surviving MNU-treated animal developed intestinal-type gastric adenocarcinoma observed by endoscopy on the 940(th) day. The level of C-reactive protein level and homocysteine concentration increased while the level of folic acid decreased with the presence of tumors in ACP03-inoculated animals and MNU treatment. ACP03 inoculation also led to anemia and leukocytosis. The hematologic and biochemical results corroborate those observed in patients with gastric cancer, supporting that our in vivo models are potentially useful to study this neoplasia. In cell line inoculated animals, we detected MYC immunoreactivity, mRNA overexpression, and amplification, as previously observed in vitro. In MNU-treated animals, mRNA expression and MYC copy number increased during the sequential steps of intestinal-type gastric carcinogenesis and immunoreactivity was only observed in intestinal metaplasia and gastric cancer. Thus, MYC deregulation supports the gastric carcinogenesis process. Canova immunomodulator restored several hematologic measurements and therefore, can be applied during/after chemotherapy to increase the tolerability and duration of anticancer treatments.

Assuntos

Cebus , Modelos Animais de Doenças , Neoplasias Experimentais/patologia , Neoplasias Gástricas/patologia , Animais , Linhagem Celular , Venenos de Crotalídeos/administração & dosagem , Venenos de Crotalídeos/farmacologia , Dosagem de Genes/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Imuno-Histoquímica , Hibridização in Situ Fluorescente , Metilnitrosoureia , Neoplasias Experimentais/diagnóstico por imagem , Neoplasias Experimentais/genética , Extratos Vegetais/administração & dosagem , Extratos Vegetais/farmacologia , Proteínas Proto-Oncogênicas c-myc/genética , Proteínas Proto-Oncogênicas c-myc/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Neoplasias Gástricas/diagnóstico por imagem , Neoplasias Gástricas/genética , Ultrassonografia

RESUMO

O Saimiri sciureus é um primata não humano existente na região amazônica e não consta na lista de animais em perigo de extinção, de acordo com o IBAMA. Neste trabalho, sistematizou-se os ramos colaterais viscerais da aorta abdominal de seis animais sendo três machos e três fêmeas, os quais tiveram o sistema circulatório preenchido com látex Neoprene, adicionado de contraste radiográfico. O resultado observado foi que a aorta abdominal emitiu ventralmente, como ramo colateral visceral, a artéria celíaca, que se trifurcou nas artérias gástrica esquerda, hepática e lienal. Os ramos da artéria celíaca promovem a irrigação do estômago, duodeno, fígado, pâncreas e baço. A seguir, a aorta abdominal emitiu a artéria mesentérica cranial, de calibre maior que a artéria celíaca e justaposta caudalmente. A artéria mesentérica cranial emitiu ramos que vascularizaram a parte final do duodeno, pâncreas, jejuno, íleo, ceco, cólon maior e cólon menor. A aorta abdominal emitiu lateralmente as artérias renais direita e esquerda. A artéria adrenal esquerda surgiu como um ramo colateral direto da artéria celíaca e a artéria adrenal direita surgiu da artéria renal direita. A artéria mesentérica caudal foi emitida da superfície ventral da aorta abdominal, logo abaixo das artérias renais. Em L6, a aorta abdominal se bifurcou, dando origem às artérias ilíacas externas direita e esquerda e estas deram origem à artéria ilíaca interna e femoral direita e esquerda. As artérias testiculares ou ováricas tiveram origem na artéria ilíaca interna. A continuidade da aorta abdominal deu origem à artéria sacral mediana e a continuidade desta é chamada de artéria caudal mediana. O estudo da espécie em questão é de suma importância para gerar conhecimentos a respeito dos primatas não humanos existentes em nosso país. Dessa forma, pode-se dizer que o Saimiri sciureus é um importante modelo biológico para o desenvolvimento de pesquisas biomédicas.

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The Saimiri sciureus is a non human primate that exists in the amazon region, which is not in the list of endangered animals according to IBAMA. So, this research systemized the collateral branches visceral of the abdominal aorta of these animals. It was used six animals, three males and three females, which had the circulatory system filled with latex Neoprene added of radiographic contrast. The results showed that the abdominal aorta ventrally emitted a collateral visceral branch called celiac artery, which originates three branches: left gastric, hepatic and lienal arteries. The branches of the celiac artery promoted the irrigation of the stomach, duodenum, liver, pancreas and spleen. Then the abdominal aorta emitted the cranial mesenteric artery, which was of a larger size than the celiac artery and was caudally juxtaposed. The cranial mesenteric artery supply branches that irrigate the final portion of the duodenum, pancreas, jejunum, ileum, cecum and colon. Abdominal aorta emitted laterally the right and left kidney arteries. The left adrenal artery as a collateral branch of the celiac artery and the right adrenal artery has been originated in the right renal artery. The mesenteric caudal artery was emitted from the ventral surface of the abdominal aorta, below the kidney arteries. In L6, abdominal aorta has two derivations to origin the right and left external iliac arteries and these had given origin to the internal iliac and femoral artery right and left. The testicular or ovary arteries were emitted in internal iliac arteries. Of the abdominal aorta led to origin the sacral median artery and in this sequence there is the caudal median artery. The study of this specie is very important to generate knowledge about non human primates existing in our country. It can be said that Saimiri sciureus is an important biological model for development of biomedical researches.