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1.
Mol Neurodegener ; 14(1): 14, 2019 03 27.
Artigo em Inglês | MEDLINE | ID: mdl-30917850

RESUMO

BACKGROUND: Amyotrophic lateral sclerosis (ALS) is a multifactorial fatal motoneuron disease without a cure. Ten percent of ALS cases can be pointed to a clear genetic cause, while the remaining 90% is classified as sporadic. Our study was aimed to uncover new connections within the ALS network through a bioinformatic approach, by which we identified C13orf18, recently named Pacer, as a new component of the autophagic machinery and potentially involved in ALS pathogenesis. METHODS: Initially, we identified Pacer using a network-based bioinformatic analysis. Expression of Pacer was then investigated in vivo using spinal cord tissue from two ALS mouse models (SOD1G93A and TDP43A315T) and sporadic ALS patients. Mechanistic studies were performed in cell culture using the mouse motoneuron cell line NSC34. Loss of function of Pacer was achieved by knockdown using short-hairpin constructs. The effect of Pacer repression was investigated in the context of autophagy, SOD1 aggregation, and neuronal death. RESULTS: Using an unbiased network-based approach, we integrated all available ALS data to identify new functional interactions involved in ALS pathogenesis. We found that Pacer associates to an ALS-specific subnetwork composed of components of the autophagy pathway, one of the main cellular processes affected in the disease. Interestingly, we found that Pacer levels are significantly reduced in spinal cord tissue from sporadic ALS patients and in tissues from two ALS mouse models. In vitro, Pacer deficiency lead to impaired autophagy and accumulation of ALS-associated protein aggregates, which correlated with the induction of cell death. CONCLUSIONS: This study, therefore, identifies Pacer as a new regulator of proteostasis associated with ALS pathology.


Assuntos
Esclerose Lateral Amiotrófica/metabolismo , Esclerose Lateral Amiotrófica/patologia , Autofagia/efeitos dos fármacos , Proteínas de Ligação a DNA/metabolismo , Neurônios Motores/metabolismo , Esclerose Lateral Amiotrófica/genética , Animais , Modelos Animais de Doenças , Humanos , Camundongos Transgênicos , Medula Espinal/metabolismo , Medula Espinal/patologia , Superóxido Dismutase/genética , Superóxido Dismutase/metabolismo
2.
An Med Interna ; 23(6): 269-71, 2006 Jun.
Artigo em Espanhol | MEDLINE | ID: mdl-17067218

RESUMO

OBJECTIVE: Determine nosocomial infection (NI) prevalence in seven University Hospitals of Havana. METHOD: A cross sectional survey was carry out in university hospital with more than 100 beds. Presence of NI was determined by an active screening procedure in all patients admitted in hospitals. Technical statistics of frequency distribution was used. Rates of NI were estimated for each hospital and in the special case of procedure, Odds Ratio and its confidence interval at 95 % were calculated (CI 95 %). RESULTS: Prevalence of NI was 9.2 %. The most frequent localization were: cardiovascular system (55 patients), surgical site (50 patients) and urinary tract (42 patients ). The 19.7 % of studied patient showed intravascular device. All patients showed NI associated with procedures, the highest intensity was association with an endotracheal tube (OR 7.83, IC 95 % 3.32-11.52). CONCLUSION: Nosocomial infections in this hospital is a serious health problem; it is necessary to focus our medical work in this regard for the prevention and control of NI.


Assuntos
Infecção Hospitalar/epidemiologia , Estudos Transversais , Cuba/epidemiologia , Número de Leitos em Hospital , Hospitais Universitários/estatística & dados numéricos , Humanos , Prevalência , Fatores de Risco
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