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J Immunoassay Immunochem ; 38(4): 420-429, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28422564

RESUMO

Paratuberculosis (PTB) or Johne's disease is a common ruminant infectious disease caused by Mycobacterium avium subsp. paratuberculosis (MAP). In this study, two MAP antigens were compared for their diagnostic utility to detect subclinical PTB in a sheep flock in Mexicali, Mexico. Sheep (n = 31) without clinical signs but positive on a direct fecal-polymerase chain reaction were tested with two preabsorbed in-house enzyme linked immunosorbent assays (ELISAs) using: (1) an ethanol-extracted surface lipid antigen (EVELISA) and (2) a protoplasmic antigen (ELISA-PPA). Sensitivities of the EVELISA and ELISA-PPA were 84% (95% CI; 66-95%) and 29% (95% CI; 14-48%), respectively. The EVELISA test could be a fast and effective way to identify subclinical ovine PTB for severely affected flocks.


Assuntos
Ensaio de Imunoadsorção Enzimática , Paratuberculose/diagnóstico , Paratuberculose/microbiologia , Doenças dos Ovinos/diagnóstico , Doenças dos Ovinos/microbiologia , Animais , Feminino , México , Paratuberculose/imunologia , Ovinos/imunologia , Ovinos/microbiologia , Doenças dos Ovinos/imunologia
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