RESUMO

The in vitro comet assay with the permanent fish cell line RTL-W1 and the in situ micronucleus assay using erythrocytes from indigenous tilapia (Oreochromis niloticus) were used to detect genotoxicity in Tietê River sediments (São Paulo, Brazil). Either test was successful in identifying site-specific differences in genotoxicity, with a high correlation between in situ and in vitro results indicating the relevance of the latter even for environmental studies. Discharges from São Paulo city have major impact on genotoxic effects by sediment-bound contaminants; however, overall genotoxicity decreases downstream. The high genotoxic burden of the Tietê River warrants measures to reduce the input of toxic effluents.

Assuntos

Dano ao DNA , Monitoramento Ambiental/métodos , Sedimentos Geológicos/química , Micronúcleos com Defeito Cromossômico/induzido quimicamente , Rios/química , Poluentes Químicos da Água/toxicidade , Animais , Brasil , Linhagem Celular , Ensaio Cometa , Eritrócitos/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Fígado/citologia , Testes para Micronúcleos , Oncorhynchus mykiss , Tilápia/sangue