RESUMO

O objetivo foi avaliar o efeito do uso de extrato de plantas sobre a resposta imune, o desempenho produtivo e a morfometria intestinal de frangos de corte desafiados com a vacina contra coccidiose. Foram utilizados 720 pintos de corte, machos, distribuídos em um delineamento inteiramente casualizado com quatro tratamentos e nove repetições de 20 aves cada. A partir dos 14 dias, as aves passaram a receber os tratamentos experimentais compostos por A – Dieta basal + Antibiótico promotor do crescimento (APC) ; B - Dieta basal + APC + desafio vacinal; C - Dieta basal + extrato de plantas, e D - Dieta basal + extrato de plantas + desafio vacinal. As aves dos tratamentos B e D receberam vacina oral contra coccidiose (vinte vezes a dose individual recomendada pelo fabricante). A adição de extrato de plantas à dieta de aves desafiadas com oocistos de Eimeria sp aumentou (p 0,05) pelo desafio ou pelo uso do extrato de plantas. O desafio com oocistos de Eimeria sp ou associado ao extrato de plantas resultou em menor (p <0,05) ganho de peso e pior conversão alimentar apenas na semana seguinte à inoculação. Os resultados obtidos mostraram que a adição de extrato de plantas à dieta de aves submetidas ao desafio vacinal aumentou a secreção de IgA pela mucosa intestinal e que a inclusão do extrato de plantas ou de melhorador de desempenho resultou em desempenho produtivo semelhante.

This study evaluated the effect of using plant extracts on immune response, productive performance, and intestinal morphometry of broilers challenged with coccidiosis vaccine. It was used 720 male Ross broiler chicks, distributed into a completely randomized design with four treatments and nine repetitions of 20 birds each. From the 14th day, animals received the following treatments: A - Basal diet + antibiotic growth promoter (AGP); B - Basal diet + AGP + vaccine challenge; C - Basal diet + plant extract and D - Basal diet + plant extract + vaccine challenge. Broilers of treatments B and D received oral coccidiosis vaccine (twenty times the label-recommended individual commercial dose). Adding plant extract to the diet of birds challenged with Eimeria sp increased (p 0.05) by the immune challenge or addition of the plant extract. The challenge with oocysts of Eimeria sp or associated with the plant extract resulted in a smaller (p <0.05) weight gain and worse feed conversion only in the next week of the inoculation. The results showed that the addition of plant extracts to the diet of birds submitted to vaccine challenged increased the secretion of IgA by the intestinal mucosa and that the inclusion of the plant extract or of the antibiotic growth promoter resulted in similar productive performance at the age of slaughter.

Assuntos

RESUMO

O objetivo foi avaliar o efeito do uso de extrato de plantas sobre a resposta imune, o desempenho produtivo e a morfometria intestinal de frangos de corte desafiados com a vacina contra coccidiose. Foram utilizados 720 pintos de corte, machos, distribuídos em um delineamento inteiramente casualizado com quatro tratamentos e nove repetições de 20 aves cada. A partir dos 14 dias, as aves passaram a receber os tratamentos experimentais compostos por A Dieta basal + Antibiótico promotor do crescimento (APC) ; B - Dieta basal + APC + desafio vacinal; C - Dieta basal + extrato de plantas, e D - Dieta basal + extrato de plantas + desafio vacinal. As aves dos tratamentos B e D receberam vacina oral contra coccidiose (vinte vezes a dose individual recomendada pelo fabricante). A adição de extrato de plantas à dieta de aves desafiadas com oocistos de Eimeria sp aumentou (p <0,05) a secreção de IgA pela mucosa intestinal. As lesões microscópicas da mucosa intestinal não foram influenciadas (p >0,05) pelo desafio ou pelo uso do extrato de plantas. O desafio com oocistos de Eimeria sp ou associado ao extrato de plantas resultou em menor (p <0,05) ganho de peso e pior conversão alimentar apenas na semana seguinte à inoculação. Os resultados obtidos mostraram que a adição de extrato de plantas à dieta de aves submetidas ao desafio vacinal aumentou a secreção de IgA pela mucosa intestinal e que a inclusão do extrato de plantas ou de melhorador de desempenho resultou em desempenho produtivo semelhante.(AU)

This study evaluated the effect of using plant extracts on immune response, productive performance, and intestinal morphometry of broilers challenged with coccidiosis vaccine. It was used 720 male Ross broiler chicks, distributed into a completely randomized design with four treatments and nine repetitions of 20 birds each. From the 14th day, animals received the following treatments: A - Basal diet + antibiotic growth promoter (AGP); B - Basal diet + AGP + vaccine challenge; C - Basal diet + plant extract and D - Basal diet + plant extract + vaccine challenge. Broilers of treatments B and D received oral coccidiosis vaccine (twenty times the label-recommended individual commercial dose). Adding plant extract to the diet of birds challenged with Eimeria sp increased (p <0.05) IgA secretion by the intestinal mucosa. Microscopic lesions of the intestinal mucosa were not affected (p> 0.05) by the immune challenge or addition of the plant extract. The challenge with oocysts of Eimeria sp or associated with the plant extract resulted in a smaller (p <0.05) weight gain and worse feed conversion only in the next week of the inoculation. The results showed that the addition of plant extracts to the diet of birds submitted to vaccine challenged increased the secretion of IgA by the intestinal mucosa and that the inclusion of the plant extract or of the antibiotic growth promoter resulted in similar productive performance at the age of slaughter.(AU)

Assuntos

RESUMO

Background: Arginine (Arg) is an essential amino acids for birds, especially in the starter phase. The degradation of Argproduces ornithine, a precursor of polyamines that are considered nutritionally important local factors for growth and thedevelopment of small intestinal of the newly hatched chick. The first week is a critical time intestine development andthe aggression to the mucosa may compromise the final productive result. This study aimed to evaluate the productiveperformance, intestinal morphometry and integrity of broilers housed under health challenge conditions and supplementedwith L-Arginine in the pre-starter diet.Materials, Methods & Results: Three-hundred-twenty male Cobb chicks were randomly assigned according to a completelyrandomized design with 4 treatments and 5 replications of 16 birds each. Treatments consisted of: A: control (basal diet);B: diet with 1% L-Arginine; C: health challenge (reused poultry manure litter) + basal diet; D: health challenge (reusedpoultry manure litter) + diet with 1% L-Arginine. The reused poultry manure litter was obtained from a commercial poultryfarm with birds in the final growth phase and without any previous treatment. In order to achieve the supplemented dietsit was added 1% Arg replacing the inert. At the ages of seven days, all the birds and the feed remains were weighed inorder to determine body weight gain, feed intake and feed conversion ratio. The small intestine of twenty birds per treatment was weighed and measured; samples of duodenum, jejunum and ileum were taken for injury assessment scores, formorphometric study and counting of goblet cells in segments of the duodenum...(AU)

Assuntos

RESUMO

Background: Arginine (Arg) is an essential amino acids for birds, especially in the starter phase. The degradation of Argproduces ornithine, a precursor of polyamines that are considered nutritionally important local factors for growth and thedevelopment of small intestinal of the newly hatched chick. The first week is a critical time intestine development andthe aggression to the mucosa may compromise the final productive result. This study aimed to evaluate the productiveperformance, intestinal morphometry and integrity of broilers housed under health challenge conditions and supplementedwith L-Arginine in the pre-starter diet.Materials, Methods & Results: Three-hundred-twenty male Cobb chicks were randomly assigned according to a completelyrandomized design with 4 treatments and 5 replications of 16 birds each. Treatments consisted of: A: control (basal diet);B: diet with 1% L-Arginine; C: health challenge (reused poultry manure litter) + basal diet; D: health challenge (reusedpoultry manure litter) + diet with 1% L-Arginine. The reused poultry manure litter was obtained from a commercial poultryfarm with birds in the final growth phase and without any previous treatment. In order to achieve the supplemented dietsit was added 1% Arg replacing the inert. At the ages of seven days, all the birds and the feed remains were weighed inorder to determine body weight gain, feed intake and feed conversion ratio. The small intestine of twenty birds per treatment was weighed and measured; samples of duodenum, jejunum and ileum were taken for injury assessment scores, formorphometric study and counting of goblet cells in segments of the duodenum...

Assuntos

RESUMO

O objetivo desta pesquisa foi avaliar o efeito da suplementação de levedura de cerveja e minerais orgânicos sobre o desempenho, morfometria intestinal e resposta imune humoral de frangos de corte, desafiados com a vacina de coccidiose. Foram utilizados 384 pintos de corte, distribuídos aleatoriamente em um delineamento inteiramente casualizado com quatro tratamentos (1. controle, 2. minerais orgânicos 3. levedo de cerveja, 4. associação de minerais orgânicos e levedo de cerveja) e 4 repetições de 24 aves cada. No alojamento, 12 aves de cada repetição foram identificadas com anilha e receberam a vacina contra coccidiose via ocular. Aos 7, 21, 35 e 42 dias, foi coletado sangue para análise de proteínas séricas totais e perfil eletroforético de IgA e IgG e um segmento do duodeno para análise morfométrica. A suplementação de levedo de cerveja ou de minerais orgânicos não afetou o desempenho de frangos de corte ou a produção de anticorpos. O desafio imunológico com a vacina de coccidiose não foi suficientemente eficaz em provocar uma resposta imune capaz de alterar a morfometria da mucosa duodenal e a produção de anticorpos.

The objective of this study was to evaluate the effects of beer yeast and organic minerals supplementations on the performance, intestinal morphometry and humoral immune response of broilers that were challenged with coccidiosis vaccine. Three-hundred-eighty-four chicks were used, randomly distributed in an entirely random design total with four treatments (1. control, 2. organic minerals on top, 3. beer yeast, 4. association between organic minerals and beer yeast) and four treatments with 24-bird repetition each. At housing, 12 birds of each repetition were identified and received the eye-drop vaccine against coccidiosis. At 7, 21, 35 and 42 days it was collected blood to analyze the total serum proteins and the eletrophoretic profile of IgA and IgG and a duodenal segment for morphometric analysis. Neither the beer yeast nor the organic minerals supplementation affected the broiler performance or the antibodies production. The immunological challenge like the coccidiosis vaccine was not efficacious enough to provide an immune response able to modify the duodenal mucosa morphometry and the antibodies production.