1.
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;27(1): 11-9, jan. 1994. tab, ilus
Artigo
em Inglês
| LILACS
| ID: lil-136487
RESUMO
1. Bradykinin, Lys-bradykinin, Met-Lys-bradykinin, des-Arg9- bradykinin and des-Arg1- bradykinin were separated by capillary zone electrophoresis in an apparatus constructed in our laboratory which utilizes a novel N2 pulsed laser-induced fluorescence detector. 2. Detection limits of 1.2 fmol for fluorescamine-derivatized bradykinin and 90 attomol for O-phthaldiadehyde-derivatized bradykinin were achieved. 3. This powerful analytical tool is described and its successful application to the measurements of bradykinin after enzymatic release from blood is documented