RESUMO

Mobil Crystalline Material (MCM-41) can be used for the immobilization of enzymes and the investigation of electron transfer in biological systems. Electron transfer between MCM-41 with aluminum (Al-MCM-41) and cytochrome P-450 (CYP2B4) was observed using electron paramagnetic resonance (EPR). When CYP2B4 was immobilized by adsorption, it catalyzed the conversion of aniline to p-aminophenol. The electron transfer was evidenced when the signal with a g value (also called g-factor or spectroscopic manifestation of the magnetic moment) of 1.98 increased at the same time that the signal with a g value 2.24 decreased due to the addition of NADPH to CYP2B4 immobilized on Al-MCM-41, indicating that FeIII was reduced to FeII. Therefore, it is possible that Al-MCM-41 participates in the electron transfer process in biological systems.

Assuntos

Sistema Enzimático do Citocromo P-450/química , Espectroscopia de Ressonância de Spin Eletrônica/métodos , Enzimas Imobilizadas/química , Alumínio/química , Aminofenóis/química , Compostos de Anilina/química , Biotransformação , Sistema Enzimático do Citocromo P-450/metabolismo , Transporte de Elétrons , Enzimas Imobilizadas/metabolismo , Compostos Férricos/química , Compostos Férricos/farmacocinética , NADP/química , Oxirredução , Difração de Raios X