RESUMO
Physical performance is a multifactorial and complex trait influenced by environmental and hereditary factors. Environmental factors alone have been insufficient to characterize all outstanding phenotypes. Recent advances in genomic technologies have enabled the investigation of whole nuclear and mitochondrial genome sequences, increasing our ability to understand interindividual variability in physical performance. Our objective was to evaluate the association of mitochondrial polymorphic loci with physical performance in Brazilian elite military personnel. Eighty-eight male military personnel who participated in the Command Actions Course of the Army were selected. Total DNA was obtained from blood samples and a complete mitochondrial genome (mtDNA) was sequenced using Illumina MiSeq platform. Twenty-nine subjects completed the training program (FINISHED, 'F'), and fifty-nine failed to complete (NOT_FINISHED, 'NF'). The mtDNA from NF was slightly more similar to genomes from African countries frequently related to endurance level. Twenty-two distinct mtDNA haplogroups were identified corroborating the intense genetic admixture of the Brazilian population, but their distribution was similar between the two groups (FST=0.0009). Of 745 polymorphisms detected in the mtDNA, the position G11914A within the NADPH gene component of the electron transport chain, was statistically different between F and NF groups (P=0.011; OR: 4.286; 95%CI: 1.198-16.719), with a higher frequency of the G allele in group F individuals). The high performance of military personnel may be mediated by performance-related genomic traits. Thus, mitochondrial genetic markers such as the ND4 gene may play an important role on physical performance variability.
Assuntos
DNA Mitocondrial , Militares , Brasil , DNA Mitocondrial/genética , Haplótipos/genética , Humanos , Masculino , NADP , Desempenho Físico FuncionalRESUMO
Physical performance is a multifactorial and complex trait influenced by environmental and hereditary factors. Environmental factors alone have been insufficient to characterize all outstanding phenotypes. Recent advances in genomic technologies have enabled the investigation of whole nuclear and mitochondrial genome sequences, increasing our ability to understand interindividual variability in physical performance. Our objective was to evaluate the association of mitochondrial polymorphic loci with physical performance in Brazilian elite military personnel. Eighty-eight male military personnel who participated in the Command Actions Course of the Army were selected. Total DNA was obtained from blood samples and a complete mitochondrial genome (mtDNA) was sequenced using Illumina MiSeq platform. Twenty-nine subjects completed the training program (FINISHED, 'F'), and fifty-nine failed to complete (NOT_FINISHED, 'NF'). The mtDNA from NF was slightly more similar to genomes from African countries frequently related to endurance level. Twenty-two distinct mtDNA haplogroups were identified corroborating the intense genetic admixture of the Brazilian population, but their distribution was similar between the two groups (FST=0.0009). Of 745 polymorphisms detected in the mtDNA, the position G11914A within the NADPH gene component of the electron transport chain, was statistically different between F and NF groups (P=0.011; OR: 4.286; 95%CI: 1.198-16.719), with a higher frequency of the G allele in group F individuals). The high performance of military personnel may be mediated by performance-related genomic traits. Thus, mitochondrial genetic markers such as the ND4 gene may play an important role on physical performance variability.
Assuntos
Humanos , Masculino , DNA Mitocondrial/genética , Militares , Haplótipos/genética , Brasil , Desempenho Físico Funcional , NADPRESUMO
Cataract disease results from non-amyloid aggregation of eye lens proteins and is the leading cause of blindness in the world. A variety of studies have implicated both essential and xenobiotic metals as potential etiological agents in cataract disease. Essential metal ions, such as copper and zinc, are known to induce the aggregation in vitro of human γD crystallin, one of the more abundant γ-crystallins in the core of the lens. In this study, we expand the investigation of metal-crystallin interactions to heavy metal ions, such as divalent lead, cadmium and mercury. The impact of these metal ions in the non-amyloid aggregation, protein folding and thermal stability of three homologous human lens γ-crystallins has been evaluated using turbidity assays, electron microscopy, electronic absorption and circular dichroism spectroscopies. Our results show that Hg(II) ions can induce the non-amyloid aggregation of human γC and γS crystallins, but not γD crystallin. The mechanism of Hg-induced aggregation involves direct metal-protein interactions, loss of thermal stability, partial unfolding of the N-terminal domain of these proteins, and formation of disulfide-bridged dimers. Putative Hg(II) binding sites in γ-crystallins involved in metal-induced aggregation are discussed. This study reveals that mercury ions can induce the aggregation of human lens proteins, uncovering a potential role of this heavy metal ion in the bioinorganic chemistry of cataract disease.
Assuntos
Catarata/induzido quimicamente , Mercúrio/farmacologia , gama-Cristalinas/antagonistas & inibidores , Catarata/metabolismo , Humanos , Mercúrio/química , Modelos Moleculares , Agregados Proteicos/efeitos dos fármacos , gama-Cristalinas/genética , gama-Cristalinas/metabolismoRESUMO
Our study provides an integrated analysis of the variability of greenhouse gas (GHG) footprints of field-grown tomatoes for processing. The global farm-specific data set of 890 observations across 14 countries over a three-year period (2013-2015) was obtained from farms grown under Unilever's sustainable agricultural code. It represents on average 3% of the annual global production of processing tomatoes: insights can be used to help inform corporate sourcing strategies and certification schemes. The median GHG footprint ranged from 18 in Chile to 61 kg CO2-equiv per tonne of tomatoes in India, lower than results reported in other studies. We found that footprints are more consistent within countries than between them. Using linear mixed effect models, we quantified the relative influence of environmental conditions and farm management factors. Key variables were area of production and the method of fertilizer application. GHG footprints decreased with increasing area of production to a threshold of 17.4 ha. Farms using single fertilizer application methods in general had a larger GHG footprint than those using a combination of methods. We conclude that farm management factors should be prioritized for future data collection, and more stringent guidance on acceptable practices is required if greater comparability of outcomes is needed either within a scheme, such as the Unilever's sustainable agriculture code, or between schemes.
Assuntos
Gases de Efeito Estufa , Solanum lycopersicum , Chile , Efeito Estufa , ÍndiaRESUMO
The use of bi-allelic markers such as retrotransposable element insertion polymorphisms or Innuls (for insertion/null) can overcome some limitations of short tandem repeat (STR) loci in typing forensic biological evidence. This study investigated the efficiency of the InnoTyper® 21 Innul markers in an urban admixed population sample in Rio de Janeiro (n = 40) and one highly compromised sample collected as evidence by the Rio de Janeiro police. No significant departures from Hardy-Weinberg equilibrium were detected after the Bonferroni correction (α' ≈ 0.05/20, p < 0.0025), and no significant linkage disequilibrium was observed between markers. Assuming loci independence, the cumulative random match probability (RMP) was 2.3 × 10-8. A lower mean Fis value was obtained for this sample population compared with those of three North American populations (African-American, Southwest Hispanic, US Caucasian). Principal component analysis with the three North American populations and one from 21 East Asian population showed that African Americans segregated as an independent group while US Caucasian, Southwest Hispanic, East Asian, and Rio de Janeiro populations are in a single large heterogeneous group. Also, a full Innuls profile was produced from an evidence sample, despite the DNA being highly degraded. In conclusion, this system is a useful complement to standard STR kits.
Assuntos
Genética Populacional , Retroelementos , Brasil , Impressões Digitais de DNA , Humanos , Polimorfismo Genético , Análise de Componente Principal , Grupos Raciais/genéticaRESUMO
The majority of STR loci are not ideal for the analysis of forensic samples with degraded and/or low template DNA. One alternative to overcome these limitations is the use of bi-allelic markers, which have low mutation rates and shorter amplicons. Human identification (HID) InDel marker panels have been described in several countries, including Brazil. The commercial kit available is, however, mostly suitable for Europeans, with lower discrimination power for other population groups. Recently, a combination of 49 InDel markers used in four different ethnic groups in the USA has been shown to be more informative than another panel from Portugal, already tested in a Rio de Janeiro sample. However, these 49 InDels have yet to be applied to other admixed or isolated populations. We assessed the efficiency of this panel in two urban admixed populations (Rio de Janeiro, Brazil; Tripoli, Libya) and one isolated Native Brazilian community. All markers are in Hardy-Weinberg equilibrium (HWE) after the Bonferroni correction, and no Linkage disequilibrium was detected. Assuming loci independence and no substructure effect, cumulative RMP was 2.7×10(-18), 1.5×10(-20), and 4.5×10(-20) for Native Brazilian, Rio de Janeiro, and Tripoli populations, respectively. The overall Fst value was 0.05512. Rio de Janeiro and Tripoli showed similar admixture levels, however for Native Brazilians one parental cluster represented over 60 % of the total parental population. We conclude that this panel is suitable for HID on these urban populations, but is less efficient for the isolated group.
Assuntos
Etnicidade/genética , Mutação INDEL , Brasil , Frequência do Gene , Marcadores Genéticos , Humanos , Indígenas Sul-Americanos/genética , Líbia , População UrbanaRESUMO
In 2006, reports of potential Spodoptera frugiperda resistance to TC1507 maize in Puerto Rico were received. Subsequent investigation confirmed that pest populations collected from several sites in Puerto Rico were largely unaffected by the Cry1F protein in bioassays, with resistance ratios likely in excess of 1000. Since then, we have continued monitoring populations in Puerto Rico and in southern areas of the mainland US. The majority of the collections from Puerto Rico continue to show high levels of Cry1F resistance whereas populations collected from the southern US mainland continue to show full susceptibility to Cry1F and TC1507 maize. It does not appear that resistant populations have spread to any measurable extent from Puerto Rico to mainland US, nor that local selection pressure from Cry1F-expressing maize or cotton production in the southern US has caused a measurable change in population susceptibility. Lessons learned from Puerto Rico are being applied in other parts of the Americas where TC1507 maize is grown and additional steps being taken to protect the long-term durability of Cry1F in maize in areas where similar selection pressure may be expected. Tactics include using locally-adapted germplasm that contain native Spodoptera resistance, a robust education program to teach end-users about the potential for resistance to develop appropriate crop stewardship, resistance monitoring, and the use of insecticides under high S. frugiperda pressure. Perhaps most importantly, pyramided trait products that produce two or more different Bt proteins are being introduced to further delay resistance development to Cry1F.
Assuntos
Bacillus thuringiensis/genética , Proteínas de Bactérias/metabolismo , Endotoxinas/metabolismo , Proteínas Hemolisinas/metabolismo , Resistência a Inseticidas/genética , Inseticidas/metabolismo , Spodoptera/microbiologia , Zea mays/parasitologia , Animais , Bacillus thuringiensis/patogenicidade , Toxinas de Bacillus thuringiensis , Proteínas de Bactérias/toxicidade , Bioensaio , Endotoxinas/toxicidade , Feminino , Proteínas Hemolisinas/toxicidade , Interações Hospedeiro-Parasita , Resistência a Inseticidas/efeitos dos fármacos , Inseticidas/toxicidade , Larva/crescimento & desenvolvimento , Larva/microbiologia , Longevidade/efeitos dos fármacos , Masculino , Controle Biológico de Vetores , Plantas Geneticamente Modificadas , Porto Rico , Spodoptera/crescimento & desenvolvimento , Zea mays/genética , Zea mays/metabolismoRESUMO
The objective of the study is to evaluate the viscosity of popular sclerosants and their flow hydrodynamics through a syringe/needle to further discuss Miyake's old, venous-capillary reflux theory, using additional objective data. The following sclerosing agents were tested in the study: 75% dextrose (D75%); 50% dextrose (D50%); 5% ethanolamine oleate (Etha5%); 0.5% laureth-9 (Aet0.5%) and 0.1% sodium tetradecyl sulphate (STS0.1%). Using 5 mL syringes and 27G needles, the resulting pressures and flows for each sclerosant agent were measured. To do this, a three-way stopcock was connected between the syringe and the needle so that an arm of the stopcock could be used to measure injection pressures with a digital monitor in 1 mmHg increments. Two trials were performed: in trial 1, the syringe was attached to a Samtronic 680 infusion pump and in trial 2, the solutions were injected manually. The observed sclerosant viscosities were as follows: D75%: 0.28 Poise; D50%: 0.12 Poise; Etha5%: 0.10 Poise; Aet0.5%: 0.07 Poise; and STS0.1%: 0.04 Poise. In trial 1 (constant flow), it was observed that D75%, which had the highest viscosity of the sclerosants tested, had the highest pressure readings. In trial 2 (constant pressure), the flow obtained with the D75% solution was lower than the flow of the other solutions. In conclusion, based on the rabbit study theory, vessel size and sclerosant viscosity and strength, not extravasation, play a role in causing ulceration from injection sclerotherapy. As a result, they all affect the potential of venous-capillary reflux being caused by sclerotherapy injection and, thus, the risk of postsclerotherapeutic cutaneous ulceration.
Assuntos
Modelos Biológicos , Soluções Esclerosantes/efeitos adversos , Escleroterapia/efeitos adversos , Úlcera Cutânea , Animais , Pressão , Coelhos , Soluções Esclerosantes/farmacologia , Escleroterapia/métodos , Úlcera Cutânea/induzido quimicamente , Úlcera Cutânea/patologia , Úlcera Cutânea/fisiopatologia , ViscosidadeRESUMO
OBJECTIVE: Knowing about the clinical aspects of dengue in endemic zones is essential to implementation of appropriate case management protocols and public health interventions. PATIENTS AND METHODS: The authors made a 4-year prospective, observational study of dengue-infected patients admitted to the emergency department of the Fort-de-France University Hospital. RESULTS: Two hundred and sixty-three male and 297 female patients were included. The median age was 37 years (range: 14-91). The diagnosis was based on a positive RT-PCR (463 patients) or on the presence of specific IgM (97 patients). Two hundred and seventy-seven patients (49.5%) presented with dengue fever without complications. According to WHO criteria, 95 patients (17%) developed plasma leakage, including 39 patients (7%) diagnosed with DHF, and 10 (1.8%) diagnosed with DSS. Among the other patients without plasma leakage, 84 (15%) had isolated thrombocytopenia, 14 (2.5%) had internal bleeding, and 90 (16%) had unusual manifestations. Seven patients died (1.3%): fulminant hepatitis (two), myocarditis (one), encephalitis (one), acute respiratory failure (one), gangrenous cholecystitis (one), and post-traumatic intracranial hemorrhage (one). The other patients recovered. Seven patients were pregnant (1.3%) from 6 to 27 weeks of amenorrhea and carried their pregnancy to term without complications. CONCLUSION: With this experience, we were able to develop appropriate case management protocols for patients during dengue epidemics.
Assuntos
Dengue , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Dengue/complicações , Dengue/diagnóstico , Dengue/epidemiologia , Serviço Hospitalar de Emergência , Feminino , Humanos , Masculino , Martinica , Pessoa de Meia-Idade , Estudos Prospectivos , Adulto JovemRESUMO
Given the alarming global rates of mangrove forest loss it is important that resource managers have access to updated information regarding both the extent and condition of their mangrove forests. Mexican mangroves in particular have been identified as experiencing an exceptional high annual rate of loss. However, conflicting studies, using remote sensing techniques, of the current state of many of these forests may be hindering all efforts to conserve and manage what remains. Focusing on one such system, the Teacapán-Agua Brava-Las Haciendas estuarine-mangrove complex of the Mexican Pacific, an attempt was made to develop a rapid method of mapping the current condition of the mangroves based on estimated LAI. Specifically, using an AccuPAR LP-80 Ceptometer, 300 indirect in situ LAI measurements were taken at various sites within the black mangrove (Avicennia germinans) dominated forests of the northern section of this system. From this sample, 225 measurements were then used to develop linear regression models based on their relationship with corresponding values derived from QuickBird very high resolution optical satellite data. Specifically, regression analyses of the in situ LAI with both the normalized difference vegetation index (NDVI) and the simple ration (SR) vegetation index revealed significant positive relationships [LAI versus NDVI (R (2) = 0.63); LAI versus SR (R (2) = 0.68)]. Moreover, using the remaining sample, further examination of standard errors and of an F test of the residual variances indicated little difference between the two models. Based on the NDVI model, a map of estimated mangrove LAI was then created. Excluding the dead mangrove areas (i.e. LAI = 0), which represented 40% of the total 30.4 km(2) of mangrove area identified in the scene, a mean estimated LAI value of 2.71 was recorded. By grouping the healthy fringe mangrove with the healthy riverine mangrove and by grouping the dwarf mangrove together with the poor condition mangrove, mean estimated LAI values of 4.66 and 2.39 were calculated, respectively. Given that the former healthy group only represents 8% of the total mangrove area examined, it is concluded that this mangrove system, considered one of the most important of the Pacific coast of the Americas, is currently experiencing a considerable state of degradation. Furthermore, based on the results of this investigation it is suggested that this approach could provide resource managers and scientists alike with a very rapid and effective method for monitoring the state of remaining mangrove forests of the Mexican Pacific and, possibly, other areas of the tropics.
Assuntos
Avicennia/crescimento & desenvolvimento , Monitoramento Ambiental/métodos , Geografia , Folhas de Planta/crescimento & desenvolvimento , México , Oceano Pacífico , Rhizophoraceae/crescimento & desenvolvimentoRESUMO
OBJECTIVE: To determine the safety, immunogenicity, and efficacy of revaccination of children with live attenuated influenza vaccine. STUDY DESIGN: A 2-year multicenter, double-blind, placebo-controlled, efficacy field trial of live attenuated, cold-adapted trivalent influenza vaccine administered by nasal spray to children. This report summarizes year 2 results, a year in which the epidemic strain of influenza A/Sydney was not well matched to the vaccine strains. Each year, vaccine strains were antigenically equivalent to the contemporary inactivated influenza vaccine. In year 2, a single intranasal revaccination was administered. Active surveillance for influenza was conducted during the influenza season by means of viral cultures. Influenza cases were defined as illnesses with wild-type influenza virus isolated from respiratory secretions. RESULTS: In year 2, 1358 (85%) children, 26 to 85 months of age, returned for revaccination. The intranasal vaccine was easily accepted, well tolerated, and immunogenic. Revaccination resulted in 82% to 100% of the vaccinated children in a subset studied for immunogenicity being seropositive as compared with 26% to 65% of placebo recipients, depending on the influenza strain tested. No serious adverse events were associated with the vaccine. In addition to the strains in the vaccine, antibody was induced to the variant strain A/Sydney/H3N2. In year 2, influenza A/Sydney/H3N2, a variant not contained in the vaccine, caused 66 of 70 cases of influenza A; nonetheless, intranasal vaccine was 86% efficacious in preventing A/Sydney influenza. Eight cases of lower respiratory tract disease were associated with A/Sydney influenza; all cases were in the placebo group. CONCLUSIONS: This live attenuated, cold-adapted influenza vaccine was safe, immunogenic, and efficacious against influenza A/H3N2 (including a variant, A/Sydney, not contained in the vaccine) and influenza B. The characteristics of this vaccine make it suitable for routine use in children to prevent influenza.
Assuntos
Vírus da Influenza A/imunologia , Vacinas contra Influenza/administração & dosagem , Influenza Humana/prevenção & controle , Vacinação , Administração Intranasal , Criança , Pré-Escolar , Temperatura Baixa , Método Duplo-Cego , Feminino , Humanos , Vírus da Influenza B/imunologia , Masculino , Estudos Prospectivos , Vacinas AtenuadasRESUMO
1. In a previous paper we reported evidence for the presence of mGnRH- and sGnRH-like peptides in the preoptic-hypothalamic region of the capybara Hydrochaeris hydrochaeris (Montaner et al., 1998). In that study, the presence of a cGnRH-II like molecule in olfactory bulb extracts was suggested. 2. The capybara, the largest living rodent in the world, belongs to the order Hystricomorpha, which is considered to be one of the oldest groups of rodents. Some authors consider that this group is the ancestor of all remaining rodents. 3. In this study we have characterized GnRH molecular variants found in extracts from the olfactory bulbs and the mesencephalic region of capybara. These regions represent the two GnRH neuronal systems: the terminal nerve-septopreoptic and the midbrain systems. 4. An indirect method combining reverse-phase high-performance liquid chromatography (RP-HPLC) and radioimmunoassay (RIA) was used to characterize GnRH variants. The analysis of both extracts with two different RIA systems revealed three immunoreactive GnRH peaks, coeluting with mGnRH, cIIGnRH, and sGnRH synthetic standards. These results were additionally supported by serial dilution studies with specific antisera. 5. To our knowledge this the first report on the presence of three GnRH variants in the brain of an eutherian mammal. These results suggest that, similarly to other vertebrates, the expression of multiple GnRH variants may also be a common pattern in mammals.
Assuntos
Hormônio Liberador de Gonadotropina/análogos & derivados , Hormônio Liberador de Gonadotropina/análise , Mesencéfalo/química , Bulbo Olfatório/química , Animais , Evolução Biológica , Feminino , Masculino , RoedoresRESUMO
Este relato descreve os achados anátomo-histopatológicos de três casos de proteinose alveolar pulmonar em cäes. No primeiro caso, o diagnóstico clínico foi de cinomose, no segundo, o animal era sorologicamente positivo para leishmaniose e no terceiro, o animal tinha diabetes melitus. Nenhum deles apresentou sinais clínicos de doença respiratória. A proteinose alveolar pulmonar foi diagnosticada microscopicamente e caracterizou-se por alvéolos preenchidos por material eosinofílico, acelular, amorfo e PAS-positivo. Esta lesäo ainda näo foi relatada em cäes
Assuntos
Animais , Cães , Proteinose Alveolar Pulmonar/diagnósticoRESUMO
Enigmatic glassy materials (escorias) and red bricklike materials (tierras cocidas) occur at a restricted stratigraphic level (the top of the Chapadmalal Formation). Materials from one locality near Mar del Plata are attributed to a mid-Pliocene impact event with a radiometric and magnetostratigraphic age of 3.3 million years ago (Ma). An extinction of endemic fauna (including the glyptodonts and flightless cariamid birds) correlates with the unit containing the impact glasses. Moreover, the age of the glasses is coincident within dating uncertainties with a pulselike change in the oxygen isotope marine record in the Atlantic and Pacific Oceans just before the late Pliocene deterioration of the climate.
RESUMO
The molecular variants of Gonadotropin releasing hormone (GnRH) in brain extracts of the eutherian mammal Hydrochaeris hydrochaeris (Mammalia, Rodentia) were characterized. An indirect method combining reverse-phase high-performance liquid chromatography (RP-HPLC) and radioimmunoassay (RIA) with different antisera was used. Two different forebrain regions (olfactory bulbs and preoptic-hypothalamic region) were analyzed. Characterization of RP-HPLC fractions from preoptic-hypothalamic extracts with three different RIA systems revealed two immunoreactive GnRH (ir-GnRH) peaks coeluting with mammalian GnRH (mGnRH) and salmon GnRH (sGnRH) synthetic standards. These results were additionally supported by serial dilution studies with specific antisera. Similar results were obtained from olfactory bulb extracts with the same methodology. However, a third ir-GnRH peak in a similar position to that of chicken GnRH II (cIIGnRH) synthetic standard was revealed. As far as we know, this is the first report showing chromatographic and immunological evidences for the presence of a second GnRH variant in the forebrain of an eutherian mammal.
Assuntos
Hormônio Liberador de Gonadotropina/imunologia , Prosencéfalo/química , Roedores/fisiologia , Animais , Cromatografia Líquida de Alta Pressão/métodos , Feminino , Hormônio Liberador de Gonadotropina/química , Hormônio Liberador de Gonadotropina/metabolismo , Hipotálamo/química , Hipotálamo/metabolismo , Masculino , Mamíferos/fisiologia , Bulbo Olfatório/química , Bulbo Olfatório/metabolismo , Área Pré-Óptica/química , Área Pré-Óptica/metabolismo , Prosencéfalo/metabolismo , RadioimunoensaioRESUMO
Anthropometry and body-composition measures, hematologic and biochemical measures of nutritional status, and helminthic infection were studied in the population of elderly persons (> or = 60 y of age) in a rural village in Guatemala that was 65% Mayan (indigenous) and 35% ladino (European). The population had low levels of literacy and formal education. The elderly persons were much shorter and lighter than reference populations. Anemia was present in 18% of the population, and riboflavin and vitamin B-12 deficiencies were detected in 70% and 38%, respectively. Both anthropometric and biochemical-hematologic variables were lower, on average, in the Mayan descendants than in the ladinos. When grouped by body mass index (BMI; in kg/m2), greater BMI signified higher values for almost all biochemical-hematologic measures. Sixty-five percent of the sampled population had mild-to-moderate Ascaris lumbricoides and Trichuris trichiura infections. The lifestyle in rural Guatemala is evolving, and the present findings provide insights into the evolution of nutritional status in the growing number of elderly in the countryside.
Assuntos
Estado Nutricional , População Rural , Idoso , Antropometria , Composição Corporal , Europa (Continente)/etnologia , Feminino , Guatemala/epidemiologia , Hematócrito , Humanos , Indígenas Centro-Americanos , Masculino , Pessoa de Meia-Idade , Infecções por Nematoides/epidemiologia , População Rural/estatística & dados numéricos , Caracteres Sexuais , Vitaminas/sangueRESUMO
Milk substitutes are used for the breakfast of the recipients of the National School Feeding Programme in Chile. The major ingredients of milk substitutes are milk, sugar and cereal flours. The technical specifications to produce this type of food state, beside their proportions, that the flours must be precooked. This requisite is controlled by determining the gelatinization degree which must be 92% as a minimum. At present, a cheaper new alternative of milk substitute fabricated with uncooked flours and containing an enzymatic preparation has been presented to the Programme. It is postulated that when reconstituted in water under the manufacturer's directions (85 degrees C, 5 min settling time) precooking of the flours is reached. The objective of this work was to evaluate the in vitro digestibility, the effect of the enzymatic preparation (molecular size of starch and dextrose equivalent) and the degree of gelatinization of this product (MSE) when reconstituted under different temperatures and settling times. The same milk substitute but without the enzyme preparation was used as Control. In vitro digestibility (VD) of MSE was 93.8 when reconstituted at 85 degrees C/5 min. settling time. VD decreased at lower temperatures (p > 0.05) to 85.5% (75 degrees C), 82.2% (50 degrees C), 33.0% (40 degrees C) and 41.4% (20 degrees C). VD showed a significant increase when settling time was raised to 30 minutes but only at reconstitution temperatures > 60 degrees C. It was observed the enzyme present in MSE shows a 92.1% of the total potential activity when MSE is reconstituted at 85 degrees C/5 min. The degree of gelatinization, DG, ranged between 42.6% (40 degrees C) and 93.8% (85 degrees C) for 5 min settling time. These values increased after 30 min settling time. VD and DG values for Control were similar to MSE for all the conditions evaluated. It is concluded that both MSE and Control have a good in vitro digestibility and degree of gelatinization when the manufacturer's directions are followed. These results suggest that under these reconstitution conditions precooking of the flours could not be necessary and that the incorporation of enzyme in the formulation has the role of improving some physico chemical characteristics such as the viscosity of the substitute once reconstituted.
Assuntos
Digestão , Farinha , Leite , Animais , Humanos , Peptídeo HidrolasesRESUMO
OBJECTIVE: To describe the incidence and clinical presentation of invasive pneumococcal disease in a cohort of children infected with human immunodeficiency virus (HIV) who were prospectively followed from birth, in comparison with uninfected children born to HIV-infected mothers and control children. DESIGN: Prospective follow-up of a cohort recruited at birth and born to mothers with known HIV status. The person-years analysis method used the occurrence of invasive pneumococcal disease as the end point. SETTING: Hospital-based clinic specializing in care of HIV-at-risk and HIV-infected children in Baltimore, Md. PARTICIPANTS: Forty-one vertically HIV-infected children, 128 uninfected children born to HIV-infected mothers, and 71 control children born to mothers with negative findings for HIV but with HIV risk factors. RESULTS: Among HIV-infected children, 10 episodes of invasive pneumococcal disease occurred during the first 36 months of life compared with 4 episodes among uninfected children and 1 episode among control subjects. The relative risk for HIV-infected children versus the combined uninfected and control groups was 12.6 with a 95% confidence interval (5.4, 28.8) and a p value for difference between groups of < 0.001. The incidence rate per 100 child-years of observation during the first 36 months of life was 11.3 for HIV-infected, 1.1 for uninfected, and 0.5 for control children. Clinical and laboratory variables were not useful in identifying HIV-infected children at risk for pneumococcal disease. CONCLUSION: Practical strategies to prevent pneumococcal disease among HIV-infected children need to be developed.
Assuntos
Infecções por HIV/congênito , Infecções por HIV/complicações , HIV-1 , Infecções Pneumocócicas/etiologia , Sepse/etiologia , Pré-Escolar , Feminino , Seguimentos , Humanos , Incidência , Lactente , Infecções Pneumocócicas/epidemiologia , Estudos ProspectivosAssuntos
Neoplasias Encefálicas/fisiopatologia , Tronco Encefálico/patologia , Glioma/fisiopatologia , Distúrbios Pupilares/fisiopatologia , Visão Ocular/fisiologia , Adulto , Neoplasias Encefálicas/complicações , Neoplasias Encefálicas/patologia , Glioma/complicações , Glioma/patologia , Humanos , Imageamento por Ressonância Magnética , Masculino , Distúrbios Pupilares/complicações , Distúrbios Pupilares/patologia , Valores de ReferênciaRESUMO
The complementary feeding national programme (PNAC) delivers 2 kg-month of dried full-fat cow's milk (LP) to 0-1 year old infants. The purpose of this work was to design, produce and evaluate nutrient stability and shelf life of a modified LP milk formula (LPM), which seems nutritionally more adequate for infants and is of similar cost than LP, to be included into the PNAC. LPM has the following attributes: P% 12.0 (LP 21.8%): casein/whey proteins ratio: 40 to 50/60 to 50 (LP 80/20); more digestible fats; essential fatty acids: > or = 4% of total calories; vitamins and minerals which cover 100% daily requirements for 0 to 6 months old infants; protein and energy can be modulated between 1.5 and 2.25 g/100 ml and 50-75 kcal/100 ml respectively. Forty tons of LPM were produced in accordance with our specifications for a field study. Quality controls on the product showed the production feasibility of such a formula at a lower price than LP. To determine shelf life, periodical analysis of peroxides, available lysine, ascorbic acid and linoleic acid were carried out in LPM stored for 1 year at 20 degrees C and 37 degrees C. It was found that LPM has an optimum shelf life of 7 months when packed in the same material that is now being in use for LP.(ABSTRACT TRUNCATED AT 250 WORDS)