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Clin Diagn Lab Immunol ; 8(6): 1070-5, 2001 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-11687442

RESUMO

The dissociation-enhanced lanthanide fluorescent immunoassays (DELFIA) were developed for the detection of staphylococcal enterotoxin B, Yersinia pestis-specific F1 antigen, and Venezuelan equine encephalitis virus. These assays were compared to previously developed enzyme-linked immunosorbent assays (ELISAs) by determining the sensitivity or limit of detection (LOD), the dynamic range, and the reproducibility of each assay in a number of different sample matrices. The sensitivity and specificity of each assay were then determined by using a small panel of blinded spiked and nonspiked samples. All three DELFIAs demonstrated at least 1 log greater sensitivity than corresponding ELISAs utilizing the same reagents and showed an increase in dynamic range of at least 2 log(10) concentrations. This increased LOD resulted in higher sensitivity rates for the DELFIA. The specificity of all of the assays evaluated was 100%, and no sample matrix effects were observed in either format. However, the reproducibility of the DELFIA was poor due to randomly distributed wells exhibiting excessive background signal (hot wells), which occurred throughout the evaluation. As this technology matures, the reproducibility of these assays should improve, as will the ability to identify hot wells. Despite its sensitivity, the logistical burden associated with the DELFIA and the technical expertise required to complete assays and interpret the data limit the application of this technology to reference or large clinical laboratories.


Assuntos
Proteínas de Bactérias/análise , Vírus da Encefalite Equina Venezuelana/isolamento & purificação , Enterotoxinas/análise , Ensaio de Imunoadsorção Enzimática , Fluorimunoensaio , Animais , Linhagem Celular , Cricetinae , Encefalomielite Equina Venezuelana/diagnóstico , Európio , Peroxidase do Rábano Silvestre , Rim/citologia , Elementos da Série dos Lantanídeos , Sensibilidade e Especificidade , Infecções Estafilocócicas/diagnóstico , Yersiniose/diagnóstico
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