RESUMO

AIM: Detection-and identification of Venezuelan equine encephalomyelitis (VEE) virus RNA in biological samples by reverse-transcription polymerase chain reaction (RT-PCR) and RT-PCR in real time (rRT-PCR). MATERIALS AND METHODS: VEE, Sindbis, West Nile, Japanese and tick-borne encephalitis viruses were studied. Cell culture of chicken fibroblasts, outbred mice and rats, Javanese macaques were used in the experiments. Biological activity determination of the running culture of causative agents used in the experiments was carried out by negative colony method in monolayer cell culture under agar coating. and using intra-cerebral infection of mice. Reagent kits developed in the 48th Central Research Institute and Institute of Analytical Instrument Engineering were used during execution of experiments of VEE virus RNA detection by RT-PCR and rRT-PCR. RESULTS: VEE virus was detected in biological samples by various methods. Data from RT-PCR and rRT-PCR are in accordance with the results of virus detection in samples using sensitive animals. CONCLUSION: Use of molecular-diagnostics methods for detection in biological samples of a causative agent of a dangerous infectious disease is important for procuring biological safety of Russian Federation.

Assuntos

Vírus da Encefalite Japonesa (Espécie)/genética , Vírus da Encefalite Equina Venezuelana/genética , Vírus da Encefalite Transmitidos por Carrapatos/genética , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Sindbis virus/genética , Vírus do Nilo Ocidental/genética , Infecções por Alphavirus/diagnóstico , Infecções por Alphavirus/virologia , Animais , Animais não Endogâmicos , Galinhas , Vírus da Encefalite Japonesa (Espécie)/isolamento & purificação , Vírus da Encefalite Equina Venezuelana/isolamento & purificação , Vírus da Encefalite Transmitidos por Carrapatos/isolamento & purificação , Encefalite Japonesa/diagnóstico , Encefalite Japonesa/virologia , Encefalite Transmitida por Carrapatos/diagnóstico , Encefalite Transmitida por Carrapatos/virologia , Encefalomielite Equina Venezuelana/diagnóstico , Encefalomielite Equina Venezuelana/virologia , Fibroblastos/patologia , Fibroblastos/virologia , Humanos , Macaca mulatta , Camundongos , RNA Viral/isolamento & purificação , Ratos , Kit de Reagentes para Diagnóstico/normas , Sindbis virus/isolamento & purificação , Febre do Nilo Ocidental/diagnóstico , Febre do Nilo Ocidental/virologia , Vírus do Nilo Ocidental/isolamento & purificação