RESUMO

The number of draft genomes deposited in Genbank from the National Center for Biotechnology Information (NCBI) is higher than the complete ones. Draft genomes are assemblies that contain fragments of misassembled regions (gaps). Such draft genomes present a hindrance to the complete understanding of the biology and evolution of the organism since they lack genomic information. To overcome this problem, strategies to improve the assembly process are developed continuously. Also, the greatest challenge to the assembly progress is the presence of repetitive DNA regions. This article highlights the use of optical mapping, to detect and correct assembly errors in Corynebacterium pseudotuberculosis. We also demonstrate that choosing a reference genome should be done with caution to avoid assembly errors and loss of genetic information.

Assuntos

Mapeamento Cromossômico/métodos , Corynebacterium pseudotuberculosis/genética , Genoma Bacteriano , Inversão Cromossômica , Corynebacterium pseudotuberculosis/classificação , Bases de Dados de Ácidos Nucleicos , Sequenciamento de Nucleotídeos em Larga Escala , Alinhamento de Sequência/métodos , Análise de Sequência de DNA/métodos , Sequenciamento Completo do Genoma/métodos