RESUMO
Salmonella enterica resistance to extended-spectrum cephalosporins (ESC) conferred by cefotaximases (blaCTX-M) is a growing concern in the United States. Among food-producing animals, poultry are a major reservoir of ESC-resistant Salmonella. A retrospective study was carried out to further characterize 38 ceftiofur-resistant clinical Salmonella enterica isolates obtained from poultry during 2007-2018. Of the isolates tested, 31 displayed resistance to ceftriaxone and harbored blaCMY-2, whereas 7 isolates demonstrated resistance or reduced susceptibility to cefepime in addition to ceftriaxone resistance. These 7 isolates displayed extended-spectrum ß-lactamase activity, harbored blaCTX-M-1, and were recovered only from recent poultry diagnostic submissions made in 2011-2018 as opposed to the 31 isolates that were recovered in 2007-2018. Further characterization of the blaCTX-M-1 gene determined that it was located on conjugative IncN/ST1 and IncI1/ST87 plasmids in the isolates from commercial turkeys and broilers, respectively. These plasmids have been responsible for extensive spread of blaCTX-M-1 in livestock, poultry, and humans in Europe. Potential transfer of IncN and IncI1 plasmids and/or nontyphoidal Salmonella carrying these plasmids through the food chain, or by other means to humans, may result in treatment failures. Our study demonstrates the importance of further characterization of ceftiofur-resistant S. enterica isolates detected by veterinary diagnostic laboratories to identify the sources of blaCTX-M-1 and to mitigate the spread of ESC-resistant Salmonella in the poultry production pyramid.
Assuntos
Antibacterianos/farmacologia , Cefalosporinas/farmacologia , Doenças das Aves Domésticas/microbiologia , Salmonella enterica/enzimologia , beta-Lactamases/isolamento & purificação , Animais , Antibacterianos/uso terapêutico , Cefalosporinas/uso terapêutico , Europa (Continente) , Humanos , Testes de Sensibilidade Microbiana/veterinária , Aves Domésticas , Doenças das Aves Domésticas/tratamento farmacológico , Fatores R , Estudos Retrospectivos , Salmonella enterica/efeitos dos fármacos , Salmonella enterica/genética , beta-Lactamases/genéticaRESUMO
Shigatoxigenic and enteropathogenic Escherichia coli with virulence and multidrug resistance profile were isolated from Nile tilapia. This study finding is of great importance to public health because they help understand this pathogen epidemiology in fish and demonstrate how these animals can transmit E. coli related diseases to humans.(AU)
Assuntos
Humanos , Animais , Escherichia coli Enteropatogênica/isolamento & purificação , Ciclídeos/microbiologia , Escherichia coli Shiga Toxigênica/isolamento & purificação , Virulência/genética , Zoonoses/prevenção & controle , Reação em Cadeia da Polimerase/veterinária , FilogeniaRESUMO
ABSTRACT Shigatoxigenic and enteropathogenic Escherichia coli with virulence and multidrug resistance profile were isolated from Nile tilapia. This study finding is of great importance to public health because they help understand this pathogen epidemiology in fish and demonstrate how these animals can transmit E. coli related diseases to humans.
Assuntos
Humanos , Animais , Escherichia coli Enteropatogênica/isolamento & purificação , Escherichia coli Shiga Toxigênica/isolamento & purificação , Peixes/microbiologia , Filogenia , Contaminação de Alimentos/análise , Qualidade de Produtos para o Consumidor , Proteínas de Escherichia coli/genética , Escherichia coli Enteropatogênica/classificação , Escherichia coli Enteropatogênica/genética , Escherichia coli Shiga Toxigênica/classificação , Escherichia coli Shiga Toxigênica/genética , Carne/microbiologiaRESUMO
Shigatoxigenic and enteropathogenic Escherichia coli with virulence and multidrug resistance profile were isolated from Nile tilapia. This study finding is of great importance to public health because they help understand this pathogen epidemiology in fish and demonstrate how these animals can transmit E. coli related diseases to humans.
Assuntos
Escherichia coli Enteropatogênica/isolamento & purificação , Peixes/microbiologia , Escherichia coli Shiga Toxigênica/isolamento & purificação , Animais , Qualidade de Produtos para o Consumidor , Escherichia coli Enteropatogênica/classificação , Escherichia coli Enteropatogênica/genética , Proteínas de Escherichia coli/genética , Contaminação de Alimentos/análise , Humanos , Carne/microbiologia , Filogenia , Escherichia coli Shiga Toxigênica/classificação , Escherichia coli Shiga Toxigênica/genéticaRESUMO
Thirty Escherichia coli isolates from captive and free-living pigeons in Brazil were characterised. Virulence-associated genes identified in pigeons included those which occur relatively frequently in avian pathogenic E. coli (APEC) from commercial poultry worldwide. Eleven of 30 E. coli isolates from pigeons, belonging mainly to B1 and B2 phylogenetic groups, had high or intermediate pathogenicity for 1-day-old chicks. The frequency of multi-drug resistant (MDR) E. coli in captive pigeons was relatively high and included one isolate positive for the extended-spectrum ß-lactamase (ESBL) gene blaCTX-M-8. Pulsed field gel electrophoresis (PFGE) showed high heterogeneity among isolates. There is potential for pigeons to transmit antibiotic resistant pathogenic E. coli to other species through environmental contamination or direct contact.