RESUMO
SUMMARY: The aim of this study was to survey oral exostoses in human populations that belonged to the same region encompassing five periods over 6000 years, to determine the prevalence and its changing trend over time. A total of 306 human jaws belonging to the modern Xi'an region and four archeological sites, Banpo (6700-5600 years BP), Shaolingyuan (3000 years BP), Shanren (2200 years BP) and Chang'an (1000-1300 years BP), were investigated. The degree of buccal exostosis (BE), torus mandibularis (TM) and torus palatinus (TP) and the TP shape were recorded. The prevalence of BE, TM, and TP in the five groups was 20.8 %-62.5 %, 17.5 %-71.5 %, and 31.7 %-74.2 %, respectively. The differences in the three types of exostoses among the five groups were all statistically significant, but only TM and TP showed a decreasing trend over time. A high and quite diverse prevalence of oral exostoses was found in the five groups of samples. Decreasing trends in relation to time for TM and TP were detected.
RESUMEN: El objetivo de este estudio fue sondear las exostosis orales en poblaciones humanas que pertenecían a la misma región abarcando cinco períodos durante 6000 años, para determinar la prevalencia y su tendencia cambiante a lo largo del tiempo. Un total de 306 mandíbulas humanas pertenecientes a la moderna región de Xi'an y cuatro sitios arqueológicos, Banpo (6700-5600 años AP), Shaolingyuan (3000 años AP), Shanren (2200 años AP) y Chang'an (1000-1300 años AP) BP), fueron investigados. Se registró el grado de exostosis bucal (EO), torus mandibular (TM) y torus palatino (TP) y la forma de TP. La prevalencia de EO, TM y TP en los cinco grupos fue 20,8 % -62,5 %, 17,5 % -71,5 % y 31,7 % -74,2 %, respectivamente. Las diferencias en los tres tipos de exostosis entre los cinco grupos fueron todas estadísticamente significativas, pero solo TM y TP mostraron una tendencia decreciente con el tiempo. Se encontró una prevalencia alta y bastante diversa de exostosis oral en los cinco grupos de muestras. Se detectaron tendencias decrecientes en relación al tiempo para TM y TP.
Assuntos
Humanos , Exostose/patologia , Exostose/epidemiologia , Mandíbula/patologia , Palato/patologia , Arqueologia , China , Prevalência , Arcada Osseodentária/patologiaRESUMO
The aim of the present research was to establish a comprehensive strategy to identify the metabolites of isoimperatorin after biotransformation with rat liver microsomes in vitro, and further describe metabolic kinetic characteristics of isoimperatorin and its main metabolites. Utilizing liquid chromatography with time of flight mass spectrometry (LC-TOF-MS), 18 metabolites (M 1-18) were characterized according to the typical fragment ions and literature data. Among them, M-2, 3, 5, 9, 10, and 15 were new compounds. To further verify structures of the metabolites, five main metabolites were obtained from the magnifying biotransformation incubation system, and their chemical structures were elucidated as 8-hydroxyoxypeucedanin (M-3), hydroxypeucedanin hydrate (M-4), E-5-(4-hydroxy-3-methyl-2-alkenyloxy)-psoralen (M-11), Z-5-(4-hydroxy-3-methyl-2-alkenyloxy)-psoralen (M-12), and oxypeucedanin (M-16) by various spectroscopy methods including IR, MS and NMR. A simple new liquid chromatography with triple quadrupole tandem mass spectrometry (LC-QqQ-MS) method was developed for the simultaneous determination of isoimperatorin and its main metabolites. The analysis was performed on a Diamonsil™ ODS C18 column with acetonitrile-water containing 0.1% formic acid as mobile phase. Total run time was 20.0 min. The results suggested that the method we exhibited was successfully applied for analysis of isoimperatorin and its metabolites. The study provides essential data for proposing metabolite pathway and further pharmacological study of isoimperatorin.