RESUMO

This study aimed to assess the capacity of Morada Nova (MN) and Santa Inês (SIN) rams to maintain body and testicular homeothermy under thermal challenge. For 5 days in the summer, 16 males (SIN = 7 and MN = 9) underwent a heat tolerance test, i.e., period 1-animals maintained in the shade (11 to 12 h); period 2-animals exposed to sunlight (12 to 13 h); and period 3-animals returned to the shade (13 to 14 h). The respiratory rate, heart rate, rectal temperature, and infrared surface temperatures (IRT) of the trunk, back, eyeball, and testicles were assessed in each period. The index of capacity of tolerance to insolation (ICTI), which indicates the animals' level of adaptability, was calculated for each animal. Semen quality and testicular parenchyma integrity were assessed before and after the thermal challenge. Statistical analyses were performed at 5% significance. In period 1, the variables had baseline values for both genotypes. In period 2, the variables involved in thermolysis significantly increased (P < 0.05), which matches a thermal discomfort situation. In period 3, the variables returned to baseline values and some values were lower than those in period 1. Semen quality and testicular parenchyma integrity suffered no negative effects with the thermal challenge. IRT ocular and IRT testicular were positively correlated (P < 0.05). It is concluded that MN and SIN rams had efficient thermolytic mechanisms that favor preserving gonadal functionality. The animals were considered resilient to a thermal challenge. In addition, infrared thermography was an efficient tool to verify body and testicular thermoregulation.

Assuntos

RESUMO

This research aimed to evaluate the effect of inclusion unsaturated fatty acids (UFAs) in the buffalo diet, on the sperm quality after thawing. In parallel, it was verified the degree of association among techniques to assess plasma membrane integrity (PMI) of spermatozoa. Patterns of sperm motility (subjective and computer-assisted analysis), kinetics, morphology and plasma membrane integrity were evaluated. The hypo-osmotic swelling test, nigrosin-eosin staining (E-N) and fluorescent probes propidium iodide/Hoechst 33342 (IP-H342) were adopted to evaluate PMI. The study was planned considering two experimental groups, which were evaluated in repeated measures design. over time. Eleven Murrah buffalo bulls (3.5±1.0 years, and 536.9±73.0 kg) were divided in Control Group (CONT, n=5) and Palm Oil Group (PO, n=6). Animals were fed with forage and concentrated in a controlled manner. Animals of PO received dietary supplementation containing palm oil (2% of dry matter). Dietary consumption was daily monitored and the intake level of UFAs in PO was 55.4% higher than CONT. Semen samples were collected and evaluated every 14 days. The experiment lasted 120 days, considering four periods of 30 days each (Periods I, II, III and IV) and repeated measures structure was used in statistical analysis. Significance level adopted was 5%. There was no effect of treatment on sperm motility...(AU)

Este estudo investigou o efeito da inclusão de ácidos graxos insaturados (UFAs) na dieta sobre a qualidade do sêmen descongelado de bubalinos, além de verificar o grau de associação entre técnicas de avaliação da integridade de membrana plasmática (IMP) dos espermatozoides. Foram avaliados parâmetros de motilidade (subjetiva e computadorizada), cinética, morfologia e IMP. A IMP foi avaliada pelos testes hiposmótico, coloração de eosina-nigrosina (E-N) e sondas fluorescentes (iodeto de propídio/Hoescht 33342; IP-H342). O experimento foi delineado para execução com dois grupos experimentais, avaliados em medidas repetidas ao longo do tempo. Onze touros bubalinos Murrah (3,5±1,0 anos e 536,9±73,0 kg) foram divididos de modo inteiramente casualizado, em dois tratamentos: Grupo Controle (CONT, n=5) e Grupo Óleo de Palma (OP, n=6). Os animais receberam forragem e concentrado de maneira controlada. O grupo OP recebeu óleo de palma adicionalmente na dieta (2% da matéria seca). O consumo foi diariamente controlado e o nível de ingestão de UFAs em OP foi 55,4% superior ao CONT. As coletas seminais foram realizadas a cada 14 dias, durante 120 dias. As variáveis de qualidade do sêmen foram avaliadas em quatro períodos de tempo (Períodos I, II, III e IV), com intervalos de 30 dias cada. O nível de significância adotado foi de 5%. Não houve efeito do tratamento sobre a motilidade espermática...(AU)

Assuntos

RESUMO

This research aimed to evaluate the effect of inclusion unsaturated fatty acids (UFAs) in the buffalo diet, on the sperm quality after thawing. In parallel, it was verified the degree of association among techniques to assess plasma membrane integrity (PMI) of spermatozoa. Patterns of sperm motility (subjective and computer-assisted analysis), kinetics, morphology and plasma membrane integrity were evaluated. The hypo-osmotic swelling test, nigrosin-eosin staining (E-N) and fluorescent probes propidium iodide/Hoechst 33342 (IP-H342) were adopted to evaluate PMI. The study was planned considering two experimental groups, which were evaluated in repeated measures design. over time. Eleven Murrah buffalo bulls (3.5±1.0 years, and 536.9±73.0 kg) were divided in Control Group (CONT, n=5) and Palm Oil Group (PO, n=6). Animals were fed with forage and concentrated in a controlled manner. Animals of PO received dietary supplementation containing palm oil (2% of dry matter). Dietary consumption was daily monitored and the intake level of UFAs in PO was 55.4% higher than CONT. Semen samples were collected and evaluated every 14 days. The experiment lasted 120 days, considering four periods of 30 days each (Periods I, II, III and IV) and repeated measures structure was used in statistical analysis. Significance level adopted was 5%. There was no effect of treatment on sperm motility...

Este estudo investigou o efeito da inclusão de ácidos graxos insaturados (UFAs) na dieta sobre a qualidade do sêmen descongelado de bubalinos, além de verificar o grau de associação entre técnicas de avaliação da integridade de membrana plasmática (IMP) dos espermatozoides. Foram avaliados parâmetros de motilidade (subjetiva e computadorizada), cinética, morfologia e IMP. A IMP foi avaliada pelos testes hiposmótico, coloração de eosina-nigrosina (E-N) e sondas fluorescentes (iodeto de propídio/Hoescht 33342; IP-H342). O experimento foi delineado para execução com dois grupos experimentais, avaliados em medidas repetidas ao longo do tempo. Onze touros bubalinos Murrah (3,5±1,0 anos e 536,9±73,0 kg) foram divididos de modo inteiramente casualizado, em dois tratamentos: Grupo Controle (CONT, n=5) e Grupo Óleo de Palma (OP, n=6). Os animais receberam forragem e concentrado de maneira controlada. O grupo OP recebeu óleo de palma adicionalmente na dieta (2% da matéria seca). O consumo foi diariamente controlado e o nível de ingestão de UFAs em OP foi 55,4% superior ao CONT. As coletas seminais foram realizadas a cada 14 dias, durante 120 dias. As variáveis de qualidade do sêmen foram avaliadas em quatro períodos de tempo (Períodos I, II, III e IV), com intervalos de 30 dias cada. O nível de significância adotado foi de 5%. Não houve efeito do tratamento sobre a motilidade espermática...

Assuntos

RESUMO

Estudos relacionados à obtenção e avaliação de sêmen de Tayassu tajacu são escassos, sendo necessárias pesquisas a respeito. Os objetivos do estudo foram avaliar a biometria testicular de caititus adultos cativos, testar a eficiência da eletroejaculação para obtenção de sêmen e avaliar suas características seminais ao longo do ano. Procedeu-se à eletroejaculação em oito animais adultos e as amostras de sêmen colhidas foram avaliadas quanto às características físicas e morfológicas. Os animais tinham testículo esquerdo com 3,8 ± 0,4 cm X 2,6 ± 0,3 cm e 2,3 ± 0,2 de consistência, e testículo direito com 3,8 ± 0,5 cm X 2,7 ± 0,3 cm e 2,3 ± 0,2 de consistência. A taxa de sucesso nas colheitas foi de 75,21%. O sêmen possuiu: volume 0,81 ± 0,86 mL, concentração 137,44 ± 153 x 106 sptz mL-1, pH 7,92 ± 0,73, motilidade 52,66 ± 28,79%, vigor 2,2 ± 0,8, integridade de membrana plasmática 55,84 ± 28,55%, defeitos maiores 22,87 ± 12,93%, defeitos menores 9,11 ± 5,88% e defeitos totais 31,52 ± 13,81%. Os animais apresentaram simetria testicular, a eletroejaculação se mostrou eficiente para a obtenção de ejaculados em caititus e as flutuações observadas na produção seminal não foram suficientes para caracterizá-los como animais de reprodução sazonal.

Research development in semen collection and sperm evaluation of Tayassu tajacu are necessary. The aims of this research were to evaluate testicular biometry of captive collared peccaries, test electroejaculation for semen collection and evaluate seminal characteristics during the year. Eight animals were submitted to electroejaculation and semen samples were evaluated according their physical and morphological characteristics. Left testicles measured 3.8 ± 0.4 cm X 2.6 ± 0.3 cm and presented 2.3 ± 0.2 of consistence, while right testicles were 3.8 ± 0.5 cm X 2.7 ± 0.3 cm and presented 2.3 ± 0.2 of consistence. Success rate on semen collections achieved 75.21%. Semen presented 0.81 ± 0.86 mL (volume), 137.44 ± 153 x 106 sptz mL-1 (sperm concentration), 7.92 ± 0.73 (pH), 52.66 ± 28.79% (sperm motility), 2.2 ± 0.8 (vigour), 55.84 ± 28.55% (plasmatic membrane integrity), 22.87 ± 12.93% (primary defects), 9.11 ± 5.88% (secondary defects) and 31.52 ± 13.81% (overall defects). Seminal characteristics showed no expressive variation along the year. Testicular symmetry was observed, electroejaculation was an efficient method to semen collection and slight mensal oscillations of seminal quality were not enough to characterize collared peccaries as seasonal reproductive animals.

Assuntos