RESUMO
PURPOSE: To describe the clinical characteristics of ocular involvement in patients with pemphigus at an ophthalmological referral center. METHODS: A retrospective review was conducted on patients with the immunopathological diagnosis of pemphigus examined between 1 January 2000 and 1 April 2010. Uncorrected distance visual acuity (UDVA), best corrected distance visual acuity (BCVA), ocular symptoms, and ocular surface inflammatory and scarring changes were assessed. RESULTS: A total of 15 patients were identified, with a mean age of 68.27 ± 14.35 years, and 80% (n=12) were female. Extraocular involvement was reported in one patient. All of the eyes showed cicatricial changes in the conjunctiva. In all, 6 eyes (20%) were classified as stage I; 12 eyes (40%) as stage II; 10 eyes (33%) as stage III; and 2 eyes (7%) as stage IV. A statistically significant association was found between BCVA and the severity of ocular involvement. The mean BCVA logMAR was 1.66 (20/914), with a range from logMAR 0 (20/20) to logMAR 4 (NLP). Other ocular diseases were found in 8 (53.3%), systemic diseases in 10 (66.7%), and the use of pemphigus-inducing drugs in 10 patients (66.7%). CONCLUSIONS: The present report represents the largest series of ocular involvement in pemphigus confirmed by immunopathology. The clinical manifestations varied from conjunctival hyperemia to corneal scarring and perforation. There was a strong association between scarring changes and low BCVA. Ocular and systemic diseases as well as the use of pemphigus-inducing drugs may predispose to ocular cicatricial changes observed in this series.
Assuntos
Cicatriz/patologia , Doenças da Túnica Conjuntiva/patologia , Pênfigo/patologia , Idoso , Idoso de 80 Anos ou mais , Doenças da Túnica Conjuntiva/tratamento farmacológico , Doenças da Túnica Conjuntiva/etiologia , Feminino , Seguimentos , Humanos , Imunossupressores/uso terapêutico , Masculino , Pessoa de Meia-Idade , Pênfigo/complicações , Pênfigo/tratamento farmacológico , Estudos Retrospectivos , Índice de Gravidade de Doença , Acuidade VisualRESUMO
Allergic conjunctivitis (AC) is one of the most common eye disorders in ophthalmology. In mice models, it has been suggested that control of allergic conjunctivitis is a delicate balance between Tregs and inflammatory migrating effector cells. Our aim was to evaluate the frequency of Tregs and the frequency of homing receptors expressing cells in peripheral blood mononuclear cells (PBMC) from patients with perennial allergic conjunctivitis (PAC). The analyses of phenotypic markers on CD4+ T cells and both soluble or intracellular cytokines were performed by flow cytometry. CD4+CD25+ cells were 15 times more frequent in PBMC from patients than HC; the vast majority of these CD4+CD25+ cells were FOXP3-, and most of CD4+ T cells were CCR4+ and CCR9+ cells. Upon allergen-stimulation, no significant changes were observed in frequency of Treg; however, an increased frequency of CD4+CCR4+CCR9+ cells, CD4+CD103+ cells and CD4+CD108+ cells with increased IL-5, IL-6, and IL-8 production was observed. These findings suggest an immune dysregulation in PAC, characterized by diminished frequency of Tregs and increased frequency of circulating activated CD4+ T cells; upon allergen-stimulation, these cells were expressing cell-surface molecules related to mucosa homing and were able to trigger an inflammatory microenvironment.
Assuntos
Conjuntivite Alérgica/imunologia , Linfócitos T Auxiliares-Indutores/imunologia , Linfócitos T Reguladores/imunologia , Adolescente , Antígenos de Dermatophagoides/imunologia , Criança , Pré-Escolar , Conjuntivite Alérgica/metabolismo , Citocinas/metabolismo , Feminino , Fatores de Transcrição Forkhead , Humanos , Imunofenotipagem , Subunidade alfa de Receptor de Interleucina-2/metabolismo , Leucócitos Mononucleares/imunologia , Leucócitos Mononucleares/metabolismo , Contagem de Linfócitos , Masculino , Fenótipo , Receptores CCR/metabolismo , Receptores CCR4/metabolismo , Receptores de Quimiocinas/metabolismo , Linfócitos T Auxiliares-Indutores/metabolismo , Linfócitos T Reguladores/metabolismoRESUMO
Pterygium is one of the most frequent pathologies in ophthalmology, and is a benign, fibrovascular lesion originating from the bulbar conjunctiva. It is composed of an epithelium and highly vascular, subepithelial, loose connective tissue. The etiology of pterygium is not clearly understood; the most widely recognized originating factor is ultraviolet radiation. It has been proposed that pterygium and neoplasia have common features, raising the possibility that pterygium is a neoplastic-like growth disorder. In this study, proteomic analysis was performed to show that peroxiredoxin 2 is overexpressed in pterygia compared to healthy conjunctivas. Twelve pterygium specimens were obtained together with healthy conjunctival tissue from the same eyes. Total proteins of pterygia and healthy conjunctivas were analyzed in SDS-PAGE. This analysis showed protein bands expressed exclusively in pterygium samples at the range of 20-25 kDa. After this, 2D electrophoresis was performed for the separation of total proteins; differential spots expressed in pterygium were excised and sequenced. Mass spectrometry (MS) data were searched in the NCBInr and EST databases using the MASCOT program. The spot was identified as peroxiredoxin 2. Real-time PCR, western blot and immunohistochemistry showed that peroxiredoxin 2 was increased in pterygium compared to healthy conjunctiva. Although, these results suggest that overexpression of peroxiredoxin 2 in pterygium could protect the cell against oxidative stress-induced apoptosis, further studies are required to establish the functional role of peroxiredoxin 2 in pterygium to determine its role in peroxidation and apoptosis in this pathology.
Assuntos
Proteínas do Olho/metabolismo , Peroxirredoxinas/metabolismo , Pterígio/enzimologia , Adulto , Sequência de Aminoácidos , Western Blotting , Túnica Conjuntiva/enzimologia , Eletroforese em Gel Bidimensional , Eletroforese em Gel de Poliacrilamida , Proteínas do Olho/química , Proteínas do Olho/genética , Feminino , Humanos , Imuno-Histoquímica , Focalização Isoelétrica , Masculino , Espectrometria de Massas , Dados de Sequência Molecular , Peso Molecular , Oxirredução , Peroxirredoxinas/química , Peroxirredoxinas/genética , Proteômica , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
The aim of this work was to determine the presence of galectin-10 in nasal lavage fluid (NLF) of patients with aspirin-sensitive respiratory disease (ASRD) before and after challenge with L-ASA (aspirin) by ELISA. Fifteen ASRD patients, ten aspirin-tolerant asthmatics (ATA), and fifteen healthy controls (HC) were studied. The baseline presence of Galectin-10 in PBMC was determined using real time PCR. Galectin-10 was evaluated in tissue of nasal polyps by western blot. Our results showed a lower expression in PBMC of ASRD patients than in ATA and healthy controls. However, a higher concentration of galectin-10 in NLF was found in ASRD patients before and after L-ASA challenge; western blot confirmed a high expression of galectin-10 in tissue from nasal polyps obtained from ASRD patients. Our results suggest a probable role of galectin-10 in the inflammatory response observed in ASRD patients; however, confirmatory studies are needed.
Assuntos
Aspirina/efeitos adversos , Galectinas/metabolismo , Líquido da Lavagem Nasal/química , Doenças Respiratórias/induzido quimicamente , Doenças Respiratórias/metabolismo , Adolescente , Adulto , Idoso , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Adulto JovemRESUMO
The genetic and immunophenotypic characteristics of a 3-year-old patient with Blau syndrome (BS), an early onset sarcoidosis caused by mutations in NOD2, were investigated. Molecular analysis of NOD2 gene was achieved by PCR and direct nucleotide sequencing. Immunophenotyping included cytometric analysis of memory-effector markers on T-cells, and cytokine in serum, aqueous humour and vitreous. A novel M513R mutation in NOD2 was demonstrated. Immunophenotyping revealed higher frequency of CCR4+ cells and CCR9+ cells on CD4+ cells; most CD8+ cells were CCR7- and CCR9+. IL6 and IL-8 were detected in a gradient manner: vitreous humour>aqueous humour>serum. The immunophenotype in this patient was characterized by a differential expression of chemokine receptors on T cells and by a particular ocular microenvironment enriched in IL-6 and IL-8. To our knowledge, this is the first study analysing the immunological features of BS at aqueous humour, vitreous and blood levels. Our results expand the knowledge of the genetic and immunopathological basis of BS.
Assuntos
Humor Aquoso/imunologia , Doenças dos Nervos Cranianos/genética , Doenças dos Nervos Cranianos/imunologia , Imunofenotipagem , Leucócitos Mononucleares/imunologia , Mutação/genética , Proteína Adaptadora de Sinalização NOD2/genética , Sinovite/genética , Sinovite/imunologia , Uveíte/genética , Uveíte/imunologia , Artrite , Sequência de Bases , Pré-Escolar , Doenças dos Nervos Cranianos/patologia , Citocinas/biossíntese , Citocinas/imunologia , Feminino , Heterozigoto , Humanos , Leucócitos Mononucleares/metabolismo , Fenótipo , Receptores de Quimiocinas/genética , Receptores de Quimiocinas/imunologia , Sarcoidose , Sinovite/patologia , Receptores Toll-Like/imunologia , Receptores Toll-Like/metabolismo , Uveíte/patologia , Corpo Vítreo/imunologiaRESUMO
We purified a 70 kDa O-glycoprotein that binds to the GalNAc specific lectin from Amaranthus leucocarpus (ALLr) and determined its expression pattern on T lymphocytes from different murine lymphoid organs. High level of ALLr expression was demonstrated in 95-98% of both CD4(+)8(+) and CD4(-)8(+) thymocytes, and in 80-95% of CD8(+) T cells from peripheral blood, lymph nodes, and spleen, whereas a minor fraction of CD4(+)8(-) thymocytes (46-67%) and peripheral CD4(+) T cells (9-40%) showed low ALLr expression. Peripheral CD19(+) B cells were ALLr negative and most of the peripheral ALL(+) T cells showed a CD62L(hi)CD45RB(hi)CD44(lo/-) phenotype, indicating features of naive cells. Mitogenic activation of peripheral T cells increased 3-fold the number of ALL(+)CD4(+) T cells 24 h after stimulation, as opposed to a >80% decrease in CD8(+) T cells 72 h after stimulation. Our results suggest that ALL detects a non-described surface O-glycoprotein selectively expressed by naive CD8(+) T cells and by early activated CD4(+) T cells.
Assuntos
Glicoproteínas/metabolismo , Ativação Linfocitária , Glicoproteínas de Membrana/isolamento & purificação , Lectinas de Plantas/metabolismo , Receptores Mitogênicos/isolamento & purificação , Subpopulações de Linfócitos T/química , Animais , Antígenos CD/análise , Linfócitos T CD4-Positivos/química , Linfócitos T CD4-Positivos/metabolismo , Linfócitos T CD8-Positivos/química , Linfócitos T CD8-Positivos/metabolismo , Diferenciação Celular , Linhagem da Célula , Cromatografia de Afinidade , Regulação da Expressão Gênica , Glicosilação , Imunofenotipagem , Masculino , Glicoproteínas de Membrana/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Peso Molecular , Processamento de Proteína Pós-Traducional , Receptores Mitogênicos/metabolismo , Ácidos Siálicos/análise , Subpopulações de Linfócitos T/metabolismoAssuntos
Anticorpos Antibacterianos/imunologia , Anticorpos Antibacterianos/sangue , Antígenos de Bactérias/imunologia , Especificidade de Anticorpos , Focalização Isoelétrica , Fragmentos Fab das Imunoglobulinas , Fragmentos Fab das Imunoglobulinas/imunologia , Hanseníase/imunologia , Imunoglobulina G , Mycobacterium leprae/imunologia , Mycobacterium tuberculosis/imunologia , Tuberculose Pulmonar/imunologiaRESUMO
El asma es una enfermedad atópica caracterizada por un incremento de los eosinófilos y de la inmunoglobulina E (IgE) en la mucosa nasal y en la sangre periférica. Con los avances en el conocimiento de la fisiopatología y de las técnicas de laboratorio, en estos momentos se pueden determinar algunas proteínas de la superficie de los linfocitos T llamadas CD, por ejemplo CD62L, CD152, CD11a. Material y métodos: se reclutaron 15 sujetos distribuidos en tres grupos de cinco personas (control, asma leve y asma severa). Se determinaron CD11a, CD30, CD62L. Se efectuó un Análisis con métodos estadísticos no paramétricos. Resultados: se encontraron diferencias con respecto a CD62L (p = 0.009) relacion ndose con asma severa. Conclusión: se demuestra la existencia de un cambio en la expresión de los CD. Hace falta un número mayor de pacientes para apoyar aún más nuestros resultados.
Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Antígenos de Diferenciação de Linfócitos T/análise , Asma , Linfócitos T CD4-Positivos , Contagem de LinfócitosRESUMO
The allergic condition is determined genetically and they affect of the general population's 20-30% in developed countries, in the last decade have been increased the prevalence. Inside the imbalance that is manifested in the atopic patients it is on one hand the antigen-presenting cells (monocytes and B cells) and on the other hand, the lymphocytes T CD4+. The association of molecules like CD80, CD 86 (co-stimulatory molecules) in monocytes and B cells and CD30, CD62L, ALL, CD11a, CD28, CD124 and CD152 in CD4+, they have shown to be of particular interest in allergic sufferings. However we don't find a difference statistically significant among patient and controls and among nasal challenges with saline solution with specific allergen. For what we suggest that the changes in the activation, proliferation and cooperation are given in the les ion place, without an apparent repercussion in cells of peripheral blood.
Assuntos
Alérgenos/imunologia , Antígenos de Superfície/imunologia , Glicoproteínas/imunologia , Rinite Alérgica Perene/imunologia , Adulto , Antígenos de Dermatophagoides , Linfócitos B/imunologia , Feminino , Humanos , Macrófagos/imunologia , Masculino , Linfócitos T/imunologiaRESUMO
Se analizan 32 casos de drenaje venosos anomalo pulmonar parcial, comprobados por cirugia. El cuadro clinico y hemodinamico de esta entidad es indistinguible del cuadro clinico y hemodinamico de la comunicacion interauricular y el angiocardiograma en su fase de retorno por las venas pulmonares, puede, en gran porcentaje, proporcionar el diagnostico y el tipo de retorno. La cirugia de esta entidad debera ser indicada una vez que se llegue al diagnostico y si el paciente tiene tres anos de edad o mas
Assuntos
Pré-Escolar , Criança , Humanos , Masculino , Feminino , Malformações Arteriovenosas , Cineangiografia , Artéria PulmonarRESUMO
Se informa de tres casos de transposicion corregida de los grandes vasos que acompana a la comunicacion interventricular, y que fueron enmendados por el ventriculo sistemico, con dos sobrevivientes. No se encontro evidencia de lesion en los tejidos de conduccion de los pacientes que sobrevivieron. Esta via de acceso ofrece la ventaja de poder cerrar la comunicacion interventricular con los tejidos de conduccion lejanos al borde por este ventriculo, especialmente cuando no se tiene un aparato identificador de haz de Hiss