RESUMO
AIM: The purpose of this study was to analyse the serotype distribution of S. mutans and their association with caries activity in school children from Córdoba, Argentina. MATERIALS AND METHODS: Clinical examination was performed in 133 children. The dmft+DMFT and Significant Caries (SiC) indices were calculated to identify individuals with high caries activity. After DNA extractions of S. mutans strains, serotypes were determined by PCR amplifications. The median caries activity of each serotype group was compared using a non-parametric Kruskall-Wallis test. RESULTS: We obtained S. mutans strains from stimulated saliva of 94 children. The mean dmft+DMFT was 4.14 and the mean SiC index was 8.65. Serotype c was the most frequent (53.2%), followed by e (31.9%), f (8.5%) and k (6.4%). The comparison between the SiC and Non-Sic groups showed significant differences in the frequency of serotypes c and k. The median caries activity was non-significant in the different serotypes. CONCLUSION: The difference between the serotype frequencies detected in Argentina compared to those of other countries could be related with contrasting dietary habits. The results obtained in the present study would increase the knowledge about the epidemiology of dental caries in children from Argentina.
Assuntos
Cárie Dentária/microbiologia , Streptococcus mutans/classificação , Argentina , Criança , Feminino , Genes Bacterianos , Humanos , Masculino , Reação em Cadeia da Polimerase , Streptococcus mutans/genéticaRESUMO
The objective of our study was to validate the diagnostic utility of cardiac troponine T in acute ischemic syndromes, and also in cases of difficult diagnosis. We analyzed its concordance and compare them with conventional enzymatic quantitative methods. We determined sensitivity, specificity, positive and negative predictive values and likelihood ratio. Kappa index was used to know the concordance grade between T troponin and the positive or negative results of the quantitative enzymatic curve. Stochastic significance was valued by Chi square of Mcnemar test. In seventy patients who arrived to the hospital with chest pain who were assigned to five different groups. The sensitivity in quantitative markers was higher than qualitative methods, however the specificity, likelihood ratio was lower. In the total group the concordance analysis between qualitative and quantitative markers was adequate, (kappa index 0.65 p < 0.05). This study suggest that the rapid bedside qualitative test by cardiac Troponin T is a good diagnostic marker compared with conventional quantitative markers to evaluate chest pain in acute ischemic syndromes.
Assuntos
Creatina Quinase/análise , Isquemia Miocárdica/diagnóstico , Miocárdio/metabolismo , Mioglobina/análise , Sistemas Automatizados de Assistência Junto ao Leito , Troponina T/análise , Doença Aguda , Ensaios Enzimáticos Clínicos , Feminino , Humanos , Isoenzimas , Masculino , Pessoa de Meia-Idade , Miocárdio/enzimologiaRESUMO
We studied 310 strains of Staphylococcus spp. from neonates admitted in intensive care unit from june 1988 to may 1990, with the purpose of establishing a relationship between the slime production and the occurrence of sepsis. The original technique for its determination was modified; this facilitated the performance and the reading of results. Of 105 neonates with isolation in blood, spinal fluid and/or intravascular catheter of negative-coagulase Staphylococcus (CNS), the incidence of sepsis was 57.9% when the strain was a slime-producer, and only 11.6% when the strain did not produce slime (p less than 0.001). The risk of infection was five-fold increased when the isolated was slime-producer CNS. We proved a high predictive value when the strains were identical and isolated from two samples, one of which was blood. These results show that the production of slime is a factor which strongly support the diagnosis of neonatal sepsis due to negative-coagulase Staphylococcus.