RESUMO
Neospora caninum (Apicomplexa; Sarcocystidae) is a protozoan that causes abortion in cattle, horses, sheep, and dogs as well as neurological and dermatological diseases in dogs. In the central nervous system of dogs infected with N. caninum, cysts were detected that exhibited gliosis and meningitis. Flavonoids are polyphenolic compounds that exhibit antibacterial, antiparasitic, antifungal, and antiviral properties. In this study, we investigated the effects of flavonoids in a well-established in vitro model of N. caninum infection in glial cell cultures. Glial cells were treated individually with 10 different flavonoids, and a subset of cultures was also infected with the NC-1 strain of N. caninum. All of the flavonoids tested induced an increase in the metabolism of glial cells and many of them increased nitrite levels in cultures infected with NC-1 compared to controls and uninfected cultures. Among the flavonoids tested, 3',4'-dihydroxyflavone, 3',4',5,7-tetrahydroxyflavone (luteolin), and 3,3',4',5,6-pentahydroxyflavone (quercetin), also inhibited parasitophorous vacuole formation. Taken together, our findings show that flavonoids modulate glial cell responses, increase NO secretion, and interfere with N. caninum infection and proliferation.
Assuntos
Flavonoides/farmacologia , Fatores Imunológicos/farmacologia , Neospora/efeitos dos fármacos , Neospora/crescimento & desenvolvimento , Neuroglia/efeitos dos fármacos , Neuroglia/parasitologia , Animais , Células Cultivadas , Ratos WistarRESUMO
A freqüência de anticorpos IgG anti-Neospora caninum foi estudada em 415 amostras séricas de cães domiciliados e errantes, procedentes dos municípios baianos de Salvador e Lauro de Freitas, utilizando-se a técnica de imunofluorescência indireta, com ponto de corte igual a 1:50. Anticorpos da classe IgG anti-N. caninum foram detectados em 13,3% (22/165) dos cães domiciliados e em 11,2% (28/250) dos errantes. As freqüências de soropositivos machos e fêmeas foram 8,0% (6/75) e 18,4% (14/76) nos cães domiciliados e 12,6% (17/135) e 9,6% (11/115) nos errantes, respectivamente. Não houve diferenças estatisticamente significativas entre sexo, idade, raça e a freqüência de soropositividade ao N. caninum dos cães domiciliados e errantes.(AU)
The frequency of anti-Neospora caninum IgG antibodies was studied in 415 serum samples from owned and stray dogs from Salvador and Lauro de Freitas counties. The Indirect immunofluorescence was performed using a cut-off of 1:50. Anti-N. caninum IgG antibodies were detected in 13.3% (22/165) owned dogs and in 11.2% (28/250) stray dogs. The frequencies of males and females seropositivity were 8.0% (6/75) and 18.4% (14/76) for owned dogs and 12.6 % (17/ 135) and 9.6% (11/115) in the stray dogs population, respectively. There were not a statistical significant difference between sex, age, breed and the dogs anti-N. caninum IgG antibodies incidence.(AU)
Assuntos
Animais , Cães , Neospora/imunologia , Anticorpos/análise , Estudos Transversais , Técnica Indireta de Fluorescência para Anticorpo/veterinária , Estudos Soroepidemiológicos , Cães/imunologiaRESUMO
A freqüência de anticorpos IgG anti-Neospora caninum foi estudada em 415 amostras séricas de cães domiciliados e errantes, procedentes dos municípios baianos de Salvador e Lauro de Freitas, utilizando-se a técnica de imunofluorescência indireta, com ponto de corte igual a 1:50. Anticorpos da classe IgG anti-N. caninum foram detectados em 13,3% (22/165) dos cães domiciliados e em 11,2% (28/250) dos errantes. As freqüências de soropositivos machos e fêmeas foram 8,0% (6/75) e 18,4% (14/76) nos cães domiciliados e 12,6% (17/135) e 9,6% (11/115) nos errantes, respectivamente. Não houve diferenças estatisticamente significativas entre sexo, idade, raça e a freqüência de soropositividade ao N. caninum dos cães domiciliados e errantes.
The frequency of anti-Neospora caninum IgG antibodies was studied in 415 serum samples from owned and stray dogs from Salvador and Lauro de Freitas counties. The Indirect immunofluorescence was performed using a cut-off of 1:50. Anti-N. caninum IgG antibodies were detected in 13.3% (22/165) owned dogs and in 11.2% (28/250) stray dogs. The frequencies of males and females seropositivity were 8.0% (6/75) and 18.4% (14/76) for owned dogs and 12.6 % (17/ 135) and 9.6% (11/115) in the stray dogs population, respectively. There were not a statistical significant difference between sex, age, breed and the dogs anti-N. caninum IgG antibodies incidence.